FastQCFastQC Report
Fri 27 May 2016
SRR2139559_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139559_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2706589
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33100.048.2315561
GTATCAA60500.038.9836431
TCAACGC73000.031.7251784
CAACGCA73550.031.4902675
ATCAACG73650.031.4451853
AACGCAG76250.030.431046
TATCAAC76700.030.1947572
ACGCAGA88750.026.1449787
CGCAGAG91150.025.5504658
GCAGAGT106050.021.880749
GTACATG81800.020.5055261
GAGTACT62700.019.89500812-13
TACATGG82700.019.8705482
GTGGTAT17000.019.5332151
ACATGGG84600.019.172673
TGGTATC15850.018.8016872
CAGAGTA104400.018.69885410-11
AGAGTAC96350.018.4272510-11
GTACTTT68600.017.81237814-15
AGTACTT67800.017.04842412-13
Produced by FastQC (version 0.11.5)