FastQCFastQC Report
Fri 27 May 2016
SRR2139558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4216596
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52050.053.1032641
GTATCAA96300.037.00381
TCAACGC116850.029.8774174
ATCAACG119050.029.396733
CAACGCA119500.029.1796265
TATCAAC123150.028.659742
AACGCAG124200.028.1096446
CTTATAC52600.025.1421991
ACGCAGA143350.024.4138287
CGCAGAG148550.023.4449968
TATACAC64650.023.152763
GTACATG131950.022.3655051
TACATGG134450.021.728242
ACATGGG134750.020.922493
TTATACA68600.020.7038422
GCAGAGT171950.020.2546989
GAGTACT101650.019.9130812-13
TGGTATC22850.019.1681252
GTGGTAT26250.018.9533141
CAGAGTA165950.018.6807510-11
Produced by FastQC (version 0.11.5)