##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139558_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4216596
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.634719807161986	33.0	33.0	33.0	33.0	33.0
2	32.63334381572245	33.0	33.0	33.0	33.0	33.0
3	32.63172663447008	33.0	33.0	33.0	33.0	33.0
4	36.53952216432402	37.0	37.0	37.0	37.0	37.0
5	36.55918541875959	37.0	37.0	37.0	37.0	37.0
6	36.54731518030184	37.0	37.0	37.0	37.0	37.0
7	36.51804749613195	37.0	37.0	37.0	37.0	37.0
8	36.51898545651516	37.0	37.0	37.0	37.0	37.0
9	36.55224451192384	37.0	37.0	37.0	37.0	37.0
10-11	36.48763030653162	37.0	37.0	37.0	37.0	37.0
12-13	36.48659617852884	37.0	37.0	37.0	37.0	37.0
14-15	38.53920117080223	40.0	37.0	40.0	37.0	40.0
16-17	38.473138522163374	40.0	37.0	40.0	37.0	40.0
18-19	38.484277957859845	40.0	37.0	40.0	37.0	40.0
20-21	38.48168819266611	40.0	37.0	40.0	37.0	40.0
22-23	38.4865859656888	40.0	37.0	40.0	37.0	40.0
24-25	38.46237830633653	40.0	37.0	40.0	37.0	40.0
26-27	38.346175496586156	40.0	37.0	40.0	37.0	40.0
28-29	38.292311589527586	40.0	37.0	40.0	37.0	40.0
30-31	38.23252928478752	40.0	37.0	40.0	37.0	40.0
32-33	38.15998260364224	40.0	37.0	40.0	37.0	40.0
34-35	38.11772751065443	40.0	37.0	40.0	35.0	40.0
36-37	38.07035855411321	40.0	37.0	40.0	33.0	40.0
38-39	38.00425429302379	40.0	37.0	40.0	33.0	40.0
40-41	37.95550866949797	40.0	37.0	40.0	33.0	40.0
42-43	37.90275414974903	40.0	37.0	40.0	33.0	40.0
44-45	37.85812536197983	40.0	37.0	40.0	33.0	40.0
46-47	37.79989922895665	40.0	37.0	40.0	33.0	40.0
48-49	37.79523464332188	40.0	37.0	40.0	33.0	40.0
50-51	37.70329909487799	40.0	37.0	40.0	33.0	40.0
52-53	37.57852674864466	40.0	37.0	40.0	33.0	40.0
54-55	37.42430828104099	40.0	37.0	40.0	33.0	40.0
56-57	37.33201974181263	40.0	37.0	40.0	33.0	40.0
58-59	37.19817530257477	37.0	37.0	40.0	33.0	40.0
60-61	37.04816192732575	37.0	37.0	40.0	33.0	40.0
62-63	36.85853852740165	37.0	37.0	40.0	33.0	40.0
64-65	36.68792977570082	37.0	37.0	40.0	33.0	40.0
66-67	36.48553756906941	37.0	37.0	40.0	33.0	40.0
68-69	36.208395868046225	37.0	37.0	40.0	33.0	40.0
70-71	35.98126279066092	37.0	37.0	37.0	33.0	40.0
72-73	35.66155262017489	37.0	33.0	37.0	33.0	40.0
74-75	35.573413037388036	37.0	33.0	37.0	33.0	40.0
76-77	33.898321510639526	35.0	33.0	37.0	27.0	37.0
78-79	35.1299875422109	37.0	33.0	37.0	33.0	37.0
80-81	35.181968353259336	37.0	33.0	37.0	33.0	37.0
82-83	35.031200330623406	37.0	33.0	37.0	33.0	37.0
84-85	34.90126739498825	37.0	33.0	37.0	33.0	37.0
86-87	34.799596529269984	37.0	33.0	37.0	33.0	37.0
88-89	34.689244938654454	37.0	33.0	37.0	33.0	37.0
90-91	34.59911379591966	37.0	33.0	37.0	33.0	37.0
92-93	34.566498093201126	37.0	33.0	37.0	33.0	37.0
94-95	34.57731002634101	37.0	33.0	37.0	33.0	37.0
96-97	34.57047709554996	37.0	33.0	37.0	33.0	37.0
98-99	34.572052302829206	37.0	33.0	37.0	33.0	37.0
100	34.56967952639499	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	2.0
19	5.0
20	30.0
21	169.0
22	609.0
23	1866.0
24	4130.0
25	7915.0
26	14094.0
27	21911.0
28	32236.0
29	45012.0
30	60803.0
31	80679.0
32	106480.0
33	141509.0
34	196381.0
35	285207.0
36	504714.0
37	1226765.0
38	1438712.0
39	47365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.67261933559677	20.03175072973555	14.205297353599919	24.09033258106776
2	14.630521871196578	20.59483526522342	42.044103822135206	22.730539041444807
3	18.28337360278291	25.96018209949447	30.299914907664853	25.456529390057764
4	12.468920427757366	17.96814302342458	38.346026036167565	31.216910512650486
5	13.502242092910965	37.4853080541745	35.40320201413652	13.60924783877801
6	30.1928380143604	37.49614618047354	18.605386904507807	13.705628900658256
7	26.63937925283807	32.4379902651333	22.740641977557253	18.18198850447138
8	24.40025081843269	36.833289221922136	20.96826444838443	17.798195511260744
9	25.437379078943813	16.91583301593656	20.969981933285492	36.676805971834135
10-11	24.337378799939906	26.60402907082019	28.645470488118136	20.41312164112177
12-13	25.16467702433271	24.1316021701912	28.72378774587125	21.979933059604843
14-15	22.337713311996698	25.48666144120706	26.304181039645115	25.871444207151136
16-17	21.9079679438106	28.710908040514198	26.986685468562793	22.39443854711241
18-19	21.942094997955696	27.73633044285011	28.745734711127174	21.57583984806702
20-21	22.6829633584131	26.883469965880302	28.697933904338917	21.735632771367676
22-23	22.406047868642652	27.021915424047883	28.54973823355501	22.022298473754454
24-25	22.266533456200733	27.168921364112812	28.61053362985037	21.954011549836082
26-27	22.135226393365638	27.482422528967916	28.543062638657883	21.839288439008566
28-29	22.0117120915582	27.330809253440226	28.64647877560284	22.01099987939874
30-31	22.48213941534733	27.079690731033295	28.554203467000843	21.883966386618532
32-33	22.13666758275649	27.314190548906097	28.550816550393115	21.998325317944296
34-35	22.27620814648194	27.174066698186643	28.624200658098964	21.925524497232452
36-37	22.164662345241247	27.31704107287818	28.443801433686	22.074495148194575
38-39	22.084911805967103	27.132477465568428	28.62257812550837	22.1600326029561
40-41	22.283086808923375	27.02811590688652	28.58461445198836	22.104182832201747
42-43	22.307514204221253	27.07389075501122	28.406025853507373	22.212569187260158
44-45	22.196010510493146	27.332289403215306	28.20718781318211	22.264512273109442
46-47	22.481509982020654	27.186240280020353	27.751623002686287	22.580626735272713
48-49	22.373622408775493	27.287324469477824	27.810499071061596	22.528554050685084
50-51	22.258605431733784	27.459456619495352	27.805610013896402	22.476327934874462
52-53	22.410568870443704	27.443348572656852	27.797708950330914	22.348373606568533
54-55	22.376939802390357	27.382889306931403	27.670333392606317	22.56983749807192
56-57	22.464039519223775	27.563627606559134	27.47527109326037	22.497061780956724
58-59	22.275161117337426	27.685590931285713	27.693841995125485	22.345405956251373
60-61	22.496899032987656	27.847742749906896	27.41328507019716	22.242073146908286
62-63	22.32423362520659	27.997216502580645	27.369043816844275	22.30950605536849
64-65	22.343728839921535	27.806176698136102	27.40387206809305	22.446222393849315
66-67	22.479785688933184	27.73020083056787	27.22904935716573	22.560964123333214
68-69	22.44485162691433	27.636560517684817	27.36593088021266	22.5526569751882
70-71	22.439439548959104	27.436329451509383	27.43231820730027	22.691912792231246
72-73	22.552565457441993	27.40442634579205	27.2818470494729	22.761161147293056
74-75	22.43068774684388	27.592630866908234	27.28461087098547	22.692070515262415
76-77	22.5800655911719	27.491060796831945	27.2475568455874	22.681316766408756
78-79	22.503948063586616	27.438747134400398	27.23791429502439	22.819390506988597
80-81	22.501198787360632	27.516948360499306	27.189020604898147	22.79283224724192
82-83	22.599614635278137	27.48216479580269	27.279402354066217	22.638818214852954
84-85	22.6897674942866	27.436707784559722	27.200107756076243	22.673416965077436
86-87	22.503296885784884	27.438573120745076	27.362192576567857	22.695937416902186
88-89	22.615788909882824	27.39649596240237	27.352157981786174	22.63555714592863
90-91	22.66585647920074	27.419554973693028	27.32795473131417	22.58663381579207
92-93	22.72201422048351	27.33982448575556	27.304586176938443	22.633575116822485
94-95	22.546093080776146	27.39482814895547	27.385389481999304	22.673689288269085
96-97	22.61487186427889	27.453656043844944	27.48146774730693	22.45000434456924
98-99	23.725750459822756	29.325268578889258	27.708959614439188	19.2400213468488
100	22.676682032929385	30.288783901189813	21.479266561450487	25.55526750443032
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	938.0
1	814.0
2	1039.0
3	1480.0
4	2020.0
5	3402.5
6	4647.0
7	5565.5
8	6286.5
9	6364.5
10	5885.0
11	5290.5
12	5635.5
13	7299.5
14	11194.0
15	16673.0
16	21312.5
17	24240.0
18	25030.0
19	23734.0
20	21435.0
21	19097.0
22	17702.0
23	18219.5
24	20646.5
25	25253.0
26	31662.0
27	38813.0
28	46797.0
29	55940.0
30	64999.5
31	73653.0
32	83445.5
33	93140.5
34	102544.0
35	111717.0
36	121856.5
37	131102.5
38	137727.0
39	143646.0
40	149702.0
41	154437.5
42	157019.5
43	160690.0
44	164145.5
45	167769.0
46	173442.5
47	178300.0
48	179040.5
49	178434.5
50	177847.0
51	173608.5
52	165663.5
53	155751.5
54	146501.5
55	138142.0
56	127765.5
57	115739.0
58	104114.0
59	91365.0
60	77019.5
61	63115.0
62	50594.0
63	39394.5
64	29386.5
65	21671.5
66	16238.5
67	12248.5
68	9298.0
69	6744.0
70	4790.5
71	3562.5
72	2511.5
73	1698.0
74	1128.5
75	714.5
76	465.5
77	302.0
78	195.5
79	126.0
80	94.5
81	64.0
82	39.0
83	28.5
84	26.5
85	22.0
86	18.5
87	14.5
88	9.0
89	6.5
90	6.0
91	5.0
92	4.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	4.74316249410662E-5
10-11	0.0015533857168199183
12-13	2.1344231223479793E-4
14-15	0.0014466645607025192
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	67.0
20-21	259.0
22-23	507.0
24-25	1312.0
26-27	1523.0
28-29	3270.0
30-31	8334.0
32-33	10296.0
34-35	14435.0
36-37	21362.0
38-39	24749.0
40-41	26569.0
42-43	30774.0
44-45	37149.0
46-47	36487.0
48-49	33025.0
50-51	28678.0
52-53	27391.0
54-55	31529.0
56-57	34406.0
58-59	34552.0
60-61	36251.0
62-63	35676.0
64-65	37726.0
66-67	40763.0
68-69	35604.0
70-71	35356.0
72-73	33520.0
74-75	34559.0
76-77	34341.0
78-79	36753.0
80-81	37496.0
82-83	36089.0
84-85	36337.0
86-87	38671.0
88-89	41776.0
90-91	43970.0
92-93	45713.0
94-95	52095.0
96-97	139122.0
98-99	254822.0
100-101	2723282.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.78197206608179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.59019466940512	83.12930271401606
2	5.666189790798432	10.174433870371653
3	1.062065538299778	2.8606301547593636
4	0.3324801170691867	1.194028823329333
5	0.14037048473169123	0.6301369469541531
6	0.06793659555839171	0.3659688914812933
7	0.03933628129332077	0.2472182235782323
8	0.022594987360584188	0.16228980192331527
9	0.017472001042235872	0.14118036385613159
>10	0.05927290320773173	0.8551331232997514
>50	0.0012832485294948653	0.0765973732584487
>100	7.670176744099322E-4	0.14613475725366626
>500	3.6365029570605325E-5	0.01694495591857879
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.3715812470533104E-5	0.0	2.3715812470533104E-5	0.0
3	0.0	2.3715812470533104E-5	0.0	4.743162494106621E-5	0.0
4	0.0	1.8972649976426484E-4	0.0	7.11474374115993E-5	0.0
5	0.0	2.6087393717586416E-4	0.0	9.486324988213242E-5	0.0
6	1.422948748231986E-4	2.6087393717586416E-4	0.0	1.1857906235266552E-4	0.0
7	1.422948748231986E-4	2.6087393717586416E-4	0.0	1.1857906235266552E-4	0.0
8	1.422948748231986E-4	2.6087393717586416E-4	0.0	1.422948748231986E-4	0.0
9	1.422948748231986E-4	3.0830556211693035E-4	0.0	1.422948748231986E-4	0.0
10-11	1.660106872937317E-4	3.0830556211693035E-4	0.0	1.8972649976426484E-4	0.0
12-13	1.8972649976426484E-4	3.0830556211693035E-4	0.0	2.1344231223479793E-4	0.0
14-15	1.8972649976426484E-4	3.320213745874634E-4	0.0	2.490160309405976E-4	0.0
16-17	2.964476558816638E-4	3.320213745874634E-4	0.0	2.845897496463973E-4	0.0
18-19	3.0830556211693035E-4	3.6759509329326305E-4	0.0	3.201634683521969E-4	3.557371870579966E-5
20-21	3.0830556211693035E-4	4.0316881199906274E-4	0.0	4.150267182343293E-4	4.743162494106621E-5
22-23	3.0830556211693035E-4	4.624583431753955E-4	0.0	6.8775856164546E-4	4.743162494106621E-5
24-25	3.0830556211693035E-4	5.217478743517283E-4	0.0	0.0014110908419967196	4.743162494106621E-5
26-27	3.320213745874634E-4	5.81037405528061E-4	0.0	0.0035336560581094322	4.743162494106621E-5
28-29	3.320213745874634E-4	5.928953117633276E-4	0.0	0.010589110268093031	4.743162494106621E-5
30-31	3.320213745874634E-4	5.928953117633276E-4	0.0	0.012699817577970476	4.743162494106621E-5
32-33	3.320213745874634E-4	6.047532179985942E-4	0.0	0.017608990759370828	4.743162494106621E-5
34-35	3.5573718705799655E-4	7.114743741159931E-4	0.0	0.023644665033121504	4.743162494106621E-5
36-37	3.5573718705799655E-4	8.063376239981255E-4	0.0	0.032727821209335685	4.743162494106621E-5
38-39	3.5573718705799655E-4	8.419113427039252E-4	0.0	0.05615904393022239	4.743162494106621E-5
40-41	3.5573718705799655E-4	0.0010197799362329234	0.0	0.08072862564969469	4.743162494106621E-5
42-43	3.5573718705799655E-4	0.00103163784246819	0.0	0.09645220931765813	4.743162494106621E-5
44-45	3.7945299952852967E-4	0.0011383589985855891	0.0	0.11610075994949481	4.743162494106621E-5
46-47	4.0316881199906274E-4	0.0012332222484677214	0.0	0.13554772617533195	4.743162494106621E-5
48-49	4.150267182343293E-4	0.0012332222484677214	0.0	0.15384447549634822	4.743162494106621E-5
50-51	4.5060043694012894E-4	0.001268795967173521	0.0	0.17138231881830746	4.743162494106621E-5
52-53	4.5060043694012894E-4	0.0012806538734087877	0.0	0.21343045432856267	7.114743741159932E-5
54-55	4.5060043694012894E-4	0.0012806538734087877	0.0	0.2631506551730353	9.486324988213242E-5
56-57	4.5060043694012894E-4	0.0012806538734087877	0.0	0.3142700889532694	1.0672115611739897E-4
58-59	4.5060043694012894E-4	0.0012806538734087877	0.0	0.3475789475681331	1.1857906235266552E-4
60-61	4.7431624941066206E-4	0.0013280854983498537	0.0	0.37040541707102126	1.1857906235266552E-4
62-63	5.928953117633276E-4	0.0013636592170556535	0.0	0.38295108186793325	1.422948748231986E-4
64-65	5.928953117633276E-4	0.0016601068729373172	0.0	0.3881329868927448	1.422948748231986E-4
66-67	6.166111242338607E-4	0.0016601068729373172	0.0	0.3937180607295553	1.422948748231986E-4
68-69	6.166111242338607E-4	0.0017075384978783833	0.0	0.3991015501603663	1.5415278105846515E-4
70-71	6.166111242338607E-4	0.001743112216584183	0.0	0.40433088681011886	1.660106872937317E-4
72-73	6.640427491749268E-4	0.0017786859352899827	0.0	0.40795940611811043	1.660106872937317E-4
74-75	6.640427491749268E-4	0.0017786859352899827	0.0	0.4111847566141029	1.660106872937317E-4
76-77	6.759006554101935E-4	0.0018024017477605157	0.0	0.4141018015479785	1.660106872937317E-4
78-79	6.8775856164546E-4	0.001826117560231049	0.0	0.4158804874832685	1.660106872937317E-4
80-81	6.8775856164546E-4	0.001826117560231049	0.0	0.4167223988259724	1.660106872937317E-4
82-83	6.8775856164546E-4	0.001826117560231049	0.0	0.41727972041902994	1.660106872937317E-4
84-85	6.8775856164546E-4	0.001826117560231049	0.0	0.4174694469187942	1.7786859352899827E-4
86-87	6.8775856164546E-4	0.001849833372701582	0.0	0.41761174179361743	1.8972649976426484E-4
88	6.8775856164546E-4	0.0018972649976426482	0.0	0.41761174179361743	1.8972649976426484E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5205	0.0	53.103264	1
GTATCAA	9630	0.0	37.0038	1
TCAACGC	11685	0.0	29.877417	4
ATCAACG	11905	0.0	29.39673	3
CAACGCA	11950	0.0	29.179626	5
TATCAAC	12315	0.0	28.65974	2
AACGCAG	12420	0.0	28.109644	6
CTTATAC	5260	0.0	25.142199	1
ACGCAGA	14335	0.0	24.413828	7
CGCAGAG	14855	0.0	23.444996	8
TATACAC	6465	0.0	23.15276	3
GTACATG	13195	0.0	22.365505	1
TACATGG	13445	0.0	21.72824	2
ACATGGG	13475	0.0	20.92249	3
TTATACA	6860	0.0	20.703842	2
GCAGAGT	17195	0.0	20.254698	9
GAGTACT	10165	0.0	19.91308	12-13
TGGTATC	2285	0.0	19.168125	2
GTGGTAT	2625	0.0	18.953314	1
CAGAGTA	16595	0.0	18.68075	10-11
>>END_MODULE