FastQCFastQC Report
Fri 27 May 2016
SRR2139557_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139557_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4428418
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56300.050.448161
GTATCAA101800.036.948791
TCAACGC123500.029.9040764
ATCAACG124450.029.6415353
CAACGCA126600.029.239865
TATCAAC127800.029.2316072
AACGCAG130450.028.442286
ACGCAGA149800.024.6831997
CGCAGAG154200.024.0065368
CTTATAC65450.023.456991
GTGGTAT27850.021.2847731
TATACAC83100.020.8355273
GCAGAGT180950.020.5047669
GTACATG137800.020.3636741
TACATGG136850.020.0375982
TGGTATC28400.019.6712722
ACATGGG138150.019.5403463
GAGTACT102700.019.53770612-13
TTATACA83450.018.9084032
CAGAGTA173850.018.52108610-11
Produced by FastQC (version 0.11.5)