##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139557_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4428418
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61324969774759	33.0	33.0	33.0	33.0	33.0
2	32.61794121512468	33.0	33.0	33.0	33.0	33.0
3	32.61663352465824	33.0	33.0	33.0	33.0	33.0
4	36.51934754126643	37.0	37.0	37.0	37.0	37.0
5	36.53644890793959	37.0	37.0	37.0	37.0	37.0
6	36.52828323794186	37.0	37.0	37.0	37.0	37.0
7	36.49809999869028	37.0	37.0	37.0	37.0	37.0
8	36.497712953022955	37.0	37.0	37.0	37.0	37.0
9	36.53691769837445	37.0	37.0	37.0	37.0	37.0
10-11	36.47008514101424	37.0	37.0	37.0	37.0	37.0
12-13	36.46587912432837	37.0	37.0	37.0	37.0	37.0
14-15	38.4955830727813	40.0	37.0	40.0	37.0	40.0
16-17	38.425282121064456	40.0	37.0	40.0	37.0	40.0
18-19	38.430596772933356	40.0	37.0	40.0	37.0	40.0
20-21	38.42854032878655	40.0	37.0	40.0	37.0	40.0
22-23	38.434587692401905	40.0	37.0	40.0	37.0	40.0
24-25	38.413955349429585	40.0	37.0	40.0	37.0	40.0
26-27	38.29025620474616	40.0	37.0	40.0	37.0	40.0
28-29	38.22957410688366	40.0	37.0	40.0	37.0	40.0
30-31	38.17010146385447	40.0	37.0	40.0	37.0	40.0
32-33	38.101659121868174	40.0	37.0	40.0	35.0	40.0
34-35	38.054487051570305	40.0	37.0	40.0	33.0	40.0
36-37	38.0076996152857	40.0	37.0	40.0	33.0	40.0
38-39	37.93061445078865	40.0	37.0	40.0	33.0	40.0
40-41	37.89279801493285	40.0	37.0	40.0	33.0	40.0
42-43	37.835136649970096	40.0	37.0	40.0	33.0	40.0
44-45	37.787399649817445	40.0	37.0	40.0	33.0	40.0
46-47	37.72813932308969	40.0	37.0	40.0	33.0	40.0
48-49	37.712868436590206	40.0	37.0	40.0	33.0	40.0
50-51	37.61765210655152	40.0	37.0	40.0	33.0	40.0
52-53	37.49746237477672	40.0	37.0	40.0	33.0	40.0
54-55	37.335617491438086	40.0	37.0	40.0	33.0	40.0
56-57	37.2427995022817	38.5	37.0	40.0	33.0	40.0
58-59	37.10920456746938	37.0	37.0	40.0	33.0	40.0
60-61	36.95540975678865	37.0	37.0	40.0	33.0	40.0
62-63	36.76357071626359	37.0	37.0	40.0	33.0	40.0
64-65	36.59638728682239	37.0	37.0	40.0	33.0	40.0
66-67	36.39768055496663	37.0	37.0	40.0	33.0	40.0
68-69	36.118671611519744	37.0	37.0	40.0	33.0	40.0
70-71	35.88959717894181	37.0	37.0	37.0	33.0	40.0
72-73	35.56964809508953	37.0	33.0	37.0	33.0	40.0
74-75	35.478751212322464	37.0	33.0	37.0	33.0	40.0
76-77	33.79542020041336	35.0	33.0	37.0	27.0	37.0
78-79	35.04414436547675	37.0	33.0	37.0	33.0	37.0
80-81	35.09245918143437	37.0	33.0	37.0	33.0	37.0
82-83	34.93876389720455	37.0	33.0	37.0	33.0	37.0
84-85	34.81991182788685	37.0	33.0	37.0	33.0	37.0
86-87	34.71949882081307	37.0	33.0	37.0	33.0	37.0
88-89	34.606150821195286	37.0	33.0	37.0	33.0	37.0
90-91	34.51403297977768	37.0	33.0	37.0	33.0	37.0
92-93	34.479709989281034	37.0	33.0	37.0	33.0	37.0
94-95	34.48836503031112	37.0	33.0	37.0	33.0	37.0
96-97	34.48148189839595	37.0	33.0	37.0	33.0	37.0
98-99	34.49271131185925	37.0	33.0	37.0	33.0	37.0
100	34.484898135075454	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	45.0
21	189.0
22	722.0
23	2067.0
24	4578.0
25	9217.0
26	15675.0
27	24354.0
28	35852.0
29	50095.0
30	67047.0
31	88140.0
32	116835.0
33	155172.0
34	213482.0
35	312149.0
36	553388.0
37	1304484.0
38	1433870.0
39	41051.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.532732456601885	19.973882320955248	14.319560619616306	24.173824602826564
2	14.681811879547052	20.43942103026408	42.02410431896899	22.85466277121988
3	18.349916380973973	25.78354166205629	30.20414965344283	25.662392303526904
4	12.545766908182559	17.896820038216806	38.08768729600503	31.4697257575956
5	13.602758366531795	37.44030035105087	35.281402975057915	13.675538307359423
6	30.466139375280292	37.29957289488029	18.46316223987889	13.771125489960523
7	26.881315178467798	32.32603606976577	22.538229227683566	18.254419524082866
8	24.472125260081594	36.811452758072974	20.820731015003552	17.895690966841883
9	25.435218185484594	16.906244700412856	20.85888143625332	36.79965567784924
10-11	24.51027802765113	26.55790090879129	28.446514388132204	20.485306675425374
12-13	25.327512889209647	23.96950962235121	28.52684149752502	22.17613599091413
14-15	22.437811091324512	25.406414380757493	26.10125369154554	26.054520836372458
16-17	22.07808747954687	28.594184198510618	26.766928957474207	22.560799364468302
18-19	22.104948087556323	27.561896821844734	28.459090808500914	21.874064282098033
20-21	22.8618834809825	26.80534698495356	28.384353779901495	21.948415754162443
22-23	22.64576031682474	26.820548711510572	28.271506572925496	22.262184398739187
24-25	22.49342822663484	26.96442517665435	28.367325848711705	22.174820747999103
26-27	22.447963448156646	27.13790208778905	28.29451846629586	22.119615997758444
28-29	22.214352061239246	26.966445946708205	28.383888818363907	22.435313173688638
30-31	22.698524045745767	26.964509268889515	28.289596598455546	22.047370086909172
32-33	22.53800216693569	26.943106882317025	28.41938993559858	22.099501015148707
34-35	22.264729342449083	27.059991050387953	28.57057915086008	22.104700456302883
36-37	22.518493700319432	27.060873328080937	28.183835551907887	22.23679741969174
38-39	22.27723357228052	27.035573866159517	28.362236088631064	22.324956472928896
40-41	22.49718232186372	26.889356036928056	28.334850596417677	22.27861104479055
42-43	22.44669603513996	26.94116653675827	28.208508030220514	22.403629397881257
44-45	22.3899668645445	27.20367401229548	27.977283360878907	22.429075762281112
46-47	22.67629334185937	27.04306905252431	27.48439074751616	22.796246858100165
48-49	22.50023947832328	27.12915292347035	27.60403486423128	22.76657273397509
50-51	22.43396631577127	27.37442875212514	27.540861066848194	22.6507438652554
52-53	22.568849948838775	27.32759392257843	27.57129310907864	22.53226301950416
54-55	22.550230816827085	27.274102794227957	27.431525803205158	22.744140585739807
56-57	22.712856132005935	27.419904081452145	27.19789588513361	22.66934390140831
58-59	22.39135017651792	27.5470021645343	27.483845170970493	22.577802487977284
60-61	22.791210791175885	27.641568116119075	27.279169694756117	22.28805139794892
62-63	22.430777823180396	27.77786936605711	27.216266816709243	22.57508599405325
64-65	22.437491112999943	27.609416998818624	27.19027462625042	22.76281726193101
66-67	22.53831752607084	27.581876062859507	27.2553748695286	22.624431541541053
68-69	22.576737583867367	27.58879787624674	27.189140936096667	22.645323603789226
70-71	22.49932729545524	27.376559698565483	27.10002376409044	23.024089241888834
72-73	22.647547757051402	27.389895659618386	26.952793524847735	23.009763058482477
74-75	22.4736874766869	27.565675377563775	27.169778082650648	22.790859063098676
76-77	22.629836344206893	27.304311181502243	27.098387476605396	22.967464997685465
78-79	22.554842701295776	27.307343998078053	26.866839335171388	23.27097396545478
80-81	22.7912186761759	27.53957652166478	26.87138809170278	22.797816710456537
82-83	22.845994880504588	27.336591194051103	26.95292773314717	22.86448619229714
84-85	22.741072201885824	27.273688201601704	26.889384286929328	23.095855309583143
86-87	22.566208703271958	27.559514620852166	26.977889948036438	22.896386727839438
88-89	22.815789964706102	27.511485946311364	26.96123699443536	22.711487094547174
90-91	22.871982440967663	27.542120590604558	26.90426429697819	22.681632671449584
92-93	22.746135389648874	27.252664569417213	26.988199106668915	23.013000934264998
94-95	22.707430298019574	27.56124013132742	27.057576416500993	22.67375315415201
96-97	23.036876897625373	27.408058322834655	27.112221830759044	22.44284294878093
98-99	23.822093934938998	29.442230374486478	27.383350097981037	19.35232559259349
100	22.69094800099136	30.479730049595798	21.191099768033126	25.638222181379717
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1185.0
1	1044.0
2	1302.0
3	1794.0
4	2365.5
5	3811.0
6	5038.5
7	5757.0
8	6230.0
9	6137.0
10	5607.0
11	5006.0
12	5282.5
13	6902.5
14	10909.0
15	16556.0
16	21350.5
17	24384.5
18	25329.0
19	24191.5
20	21908.0
21	19530.0
22	17935.5
23	18190.0
24	20535.5
25	24752.0
26	31022.0
27	38099.5
28	45276.5
29	54048.5
30	63033.0
31	72168.0
32	82766.0
33	92696.5
34	102466.0
35	112792.5
36	124180.0
37	134492.5
38	141237.5
39	147218.0
40	153873.5
41	159538.5
42	163385.5
43	167775.5
44	172560.5
45	176431.5
46	179413.5
47	181244.5
48	182822.0
49	186285.0
50	189753.0
51	187232.0
52	179581.0
53	171813.5
54	162976.5
55	152556.5
56	141155.0
57	128318.5
58	115265.0
59	100874.5
60	84774.5
61	68490.0
62	53797.0
63	41441.0
64	30809.5
65	22507.5
66	16823.5
67	12834.5
68	9748.0
69	7205.5
70	5144.0
71	3691.0
72	2671.0
73	1864.0
74	1248.0
75	807.0
76	514.5
77	325.0
78	193.5
79	119.5
80	93.5
81	70.0
82	47.0
83	33.5
84	26.5
85	23.0
86	16.0
87	11.0
88	9.0
89	9.0
90	9.0
91	8.0
92	6.5
93	3.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5806999248941722E-4
10-11	0.001433920646153999
12-13	1.0161642374319678E-4
14-15	0.0012645599399153378
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	76.0
20-21	272.0
22-23	615.0
24-25	1473.0
26-27	2067.0
28-29	3476.0
30-31	8537.0
32-33	10686.0
34-35	14717.0
36-37	21878.0
38-39	25070.0
40-41	27114.0
42-43	31863.0
44-45	37760.0
46-47	37467.0
48-49	34220.0
50-51	30073.0
52-53	28864.0
54-55	33020.0
56-57	35645.0
58-59	22948.0
60-61	38340.0
62-63	37938.0
64-65	39811.0
66-67	41870.0
68-69	36768.0
70-71	36105.0
72-73	34522.0
74-75	35291.0
76-77	35195.0
78-79	37532.0
80-81	38869.0
82-83	37126.0
84-85	37475.0
86-87	39671.0
88-89	42446.0
90-91	44561.0
92-93	46227.0
94-95	55729.0
96-97	162536.0
98-99	269741.0
100-101	2872824.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.5038263942741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.95588925506412	79.58988342911952
2	6.6885344705130105	11.705447182797773
3	1.439570477638508	3.779037754728068
4	0.4369772559460786	1.5294872777020776
5	0.20164268318842485	0.8822253171697773
6	0.09790533142257651	0.5140254674325003
7	0.05282323680710206	0.32355647402066023
8	0.030789534589268214	0.2155361671567859
9	0.022366186842904152	0.17614142375430442
>10	0.07023325915830642	0.9580904959803245
>50	0.002355039606144978	0.132867207502132
>100	8.755195270780948E-4	0.16826683240058218
>500	3.7749696485817005E-5	0.02543497023553232
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.2581427498488174E-5	0.0
2	0.0	0.0	0.0	2.2581427498488174E-5	0.0
3	0.0	0.0	0.0	2.2581427498488174E-5	0.0
4	0.0	0.0	0.0	2.2581427498488174E-5	2.2581427498488174E-5
5	0.0	0.0	0.0	2.2581427498488174E-5	2.2581427498488174E-5
6	4.516285499697635E-5	0.0	0.0	2.2581427498488174E-5	2.2581427498488174E-5
7	4.516285499697635E-5	0.0	0.0	2.2581427498488174E-5	2.2581427498488174E-5
8	4.516285499697635E-5	0.0	0.0	2.2581427498488174E-5	2.2581427498488174E-5
9	4.516285499697635E-5	9.03257099939527E-5	0.0	4.516285499697635E-5	2.2581427498488174E-5
10-11	6.774428249546451E-5	9.03257099939527E-5	0.0	4.516285499697635E-5	2.2581427498488174E-5
12-13	6.774428249546451E-5	9.03257099939527E-5	0.0	4.516285499697635E-5	2.2581427498488174E-5
14-15	9.03257099939527E-5	1.580699924894172E-4	0.0	7.90349962447086E-5	4.516285499697635E-5
16-17	9.03257099939527E-5	1.806514199879054E-4	0.0	9.03257099939527E-5	6.774428249546451E-5
18-19	9.03257099939527E-5	1.806514199879054E-4	0.0	9.03257099939527E-5	6.774428249546451E-5
20-21	1.3548856499092903E-4	2.0323284748639356E-4	0.0	1.0161642374319678E-4	6.774428249546451E-5
22-23	1.4677927874017313E-4	2.0323284748639356E-4	0.0	1.1290713749244086E-4	7.90349962447086E-5
24-25	1.5806999248941722E-4	2.0323284748639356E-4	0.0	1.1290713749244086E-4	9.03257099939527E-5
26-27	1.5806999248941722E-4	2.0323284748639356E-4	0.0	1.580699924894172E-4	9.03257099939527E-5
28-29	1.5806999248941722E-4	2.0323284748639356E-4	0.0	6.661521112054012E-4	9.03257099939527E-5
30-31	1.5806999248941722E-4	3.048492712295904E-4	0.0	0.0023710498873412583	9.03257099939527E-5
32-33	2.0323284748639356E-4	3.1613998497883445E-4	0.0	0.006108276138341051	1.1290713749244086E-4
34-35	2.2581427498488172E-4	3.9517498122354305E-4	0.0	0.0102180959430659	1.1290713749244086E-4
36-37	2.2581427498488172E-4	4.629192637190076E-4	0.0	0.016902198482618397	1.1290713749244086E-4
38-39	2.2581427498488172E-4	4.855006912174957E-4	0.0	0.035306061893886256	1.2419785124168493E-4
40-41	2.483957024833699E-4	5.871171149606925E-4	0.0	0.05605839376499689	1.3548856499092903E-4
42-43	2.483957024833699E-4	6.54861397456157E-4	0.0	0.06832010889667597	1.3548856499092903E-4
44-45	2.483957024833699E-4	7.79059248697842E-4	0.0	0.083539991030657	1.3548856499092903E-4
46-47	2.483957024833699E-4	8.806756724410388E-4	0.0	0.09883890816088274	1.5806999248941722E-4
48-49	2.483957024833699E-4	9.032570999395269E-4	0.0	0.11382168530612964	1.5806999248941722E-4
50-51	2.7097712998185806E-4	0.0010048735236827238	0.0	0.12835283390140678	1.5806999248941722E-4
52-53	2.7097712998185806E-4	0.0010161642374319677	0.0	0.1648557114527129	1.6936070623866132E-4
54-55	2.8226784373110213E-4	0.0010161642374319677	0.0	0.2102895435796711	1.9194213373714946E-4
56-57	2.9355855748034625E-4	0.001038745664930456	0.0	0.2563669463903362	2.1452356123563765E-4
58-59	2.9355855748034625E-4	0.0010500363786797	0.0	0.44468476101397836	2.2581427498488172E-4
60-61	3.3872141247732264E-4	0.0010951992336766764	0.0	0.6117421616477938	2.2581427498488172E-4
62-63	3.613028399758108E-4	0.0011290713749244087	0.0	0.6188666020235669	2.2581427498488172E-4
64-65	3.613028399758108E-4	0.001174234229921385	0.0	0.62479422674192	2.2581427498488172E-4
66-67	3.613028399758108E-4	0.001174234229921385	0.0	0.6301460250590617	2.2581427498488172E-4
68-69	3.83884267474299E-4	0.0011968156574198733	0.0	0.6355091140899527	2.2581427498488172E-4
70-71	3.83884267474299E-4	0.0011968156574198733	0.0	0.640149597440892	2.59686416232614E-4
72-73	4.7420997746825164E-4	0.0011968156574198733	0.0	0.644225545104369	3.387214124773226E-4
74-75	4.967914049667398E-4	0.0012419785124168495	0.0	0.6469804792591847	3.387214124773226E-4
76-77	4.967914049667398E-4	0.0012645599399153378	0.0	0.6501870419639699	3.387214124773226E-4
78-79	4.967914049667398E-4	0.0012645599399153378	0.0	0.6522193704388339	3.387214124773226E-4
80-81	5.19372832465228E-4	0.0012645599399153378	0.0	0.652829068981293	3.5001212622656666E-4
82-83	5.19372832465228E-4	0.0012645599399153378	0.0	0.6533258603862598	3.613028399758108E-4
84-85	5.19372832465228E-4	0.0012645599399153378	0.0	0.6537097446537341	3.613028399758108E-4
86-87	5.306635462144721E-4	0.0013548856499092903	0.0	0.6538226517912266	3.613028399758108E-4
88	5.419542599637161E-4	0.0014000485049062668	0.0	0.653845233218725	3.613028399758108E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5630	0.0	50.44816	1
GTATCAA	10180	0.0	36.94879	1
TCAACGC	12350	0.0	29.904076	4
ATCAACG	12445	0.0	29.641535	3
CAACGCA	12660	0.0	29.23986	5
TATCAAC	12780	0.0	29.231607	2
AACGCAG	13045	0.0	28.44228	6
ACGCAGA	14980	0.0	24.683199	7
CGCAGAG	15420	0.0	24.006536	8
CTTATAC	6545	0.0	23.45699	1
GTGGTAT	2785	0.0	21.284773	1
TATACAC	8310	0.0	20.835527	3
GCAGAGT	18095	0.0	20.504766	9
GTACATG	13780	0.0	20.363674	1
TACATGG	13685	0.0	20.037598	2
TGGTATC	2840	0.0	19.671272	2
ACATGGG	13815	0.0	19.540346	3
GAGTACT	10270	0.0	19.537706	12-13
TTATACA	8345	0.0	18.908403	2
CAGAGTA	17385	0.0	18.521086	10-11
>>END_MODULE