FastQCFastQC Report
Fri 27 May 2016
SRR2139556_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139556_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4149787
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55750.053.066661
GTATCAA107100.039.5708271
TCAACGC137200.030.5765084
ATCAACG137900.030.4524363
CAACGCA139450.030.052735
TATCAAC140700.029.785382
AACGCAG143400.029.1949776
ACGCAGA163800.025.5851867
CGCAGAG167000.025.0949338
ATCTCGT62750.024.16049494
GTGGTAT26300.023.8366151
TGGTATC25250.023.2973542
GCAGAGT191950.021.7440939
GTACATG146200.021.4692781
TATCTCG71200.021.3853194
TACATGG143850.021.1036222
ACATGGG145350.020.4722523
GAGTACT110500.020.26515412-13
AGAGTAC176100.019.75098610-11
CAGAGTA188400.018.84897410-11
Produced by FastQC (version 0.11.5)