FastQCFastQC Report
Fri 27 May 2016
SRR2139555_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139555_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8477946
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114850.052.5490041
GTATCAA216350.037.9626961
TCAACGC265400.030.2881664
ATCAACG269800.029.8574073
CAACGCA271150.029.6790665
TATCAAC275500.029.4407882
AACGCAG279750.028.7514426
ACGCAGA320850.025.1083187
GTGGTAT53300.024.789441
CGCAGAG328950.024.4513268
TGGTATC52450.023.240892
GTACATG282250.022.5454061
TACATGG282750.021.9025762
GCAGAGT374700.021.2956189
ACATGGG284850.021.2772563
GAGTACT224750.019.82744812-13
CATGGGG209600.019.1350944
CAGAGTA367250.018.94782810-11
AGAGTAC343100.018.92735110-11
GTACTTT250350.017.85166414-15
Produced by FastQC (version 0.11.5)