##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139555_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8477946
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6268346130065	33.0	33.0	33.0	33.0	33.0
2	32.622148218448196	33.0	33.0	33.0	33.0	33.0
3	32.61706821440004	33.0	33.0	33.0	33.0	33.0
4	36.52712461249458	37.0	37.0	37.0	37.0	37.0
5	36.54569892282871	37.0	37.0	37.0	37.0	37.0
6	36.53891602989687	37.0	37.0	37.0	37.0	37.0
7	36.507282660210386	37.0	37.0	37.0	37.0	37.0
8	36.508246101119305	37.0	37.0	37.0	37.0	37.0
9	36.54213261089419	37.0	37.0	37.0	37.0	37.0
10-11	36.477315260087764	37.0	37.0	37.0	37.0	37.0
12-13	36.472750888009905	37.0	37.0	37.0	37.0	37.0
14-15	38.512323798712565	40.0	37.0	40.0	37.0	40.0
16-17	38.449530346147526	40.0	37.0	40.0	37.0	40.0
18-19	38.46058709267551	40.0	37.0	40.0	37.0	40.0
20-21	38.45943628456837	40.0	37.0	40.0	37.0	40.0
22-23	38.46590644630702	40.0	37.0	40.0	37.0	40.0
24-25	38.44192410372693	40.0	37.0	40.0	37.0	40.0
26-27	38.324422007939134	40.0	37.0	40.0	37.0	40.0
28-29	38.27569151730999	40.0	37.0	40.0	37.0	40.0
30-31	38.210339147630876	40.0	37.0	40.0	37.0	40.0
32-33	38.137794033193515	40.0	37.0	40.0	37.0	40.0
34-35	38.09533179852437	40.0	37.0	40.0	33.0	40.0
36-37	38.04756515558225	40.0	37.0	40.0	33.0	40.0
38-39	37.990296837748005	40.0	37.0	40.0	33.0	40.0
40-41	37.935558007592334	40.0	37.0	40.0	33.0	40.0
42-43	37.872662381110445	40.0	37.0	40.0	33.0	40.0
44-45	37.82512074849954	40.0	37.0	40.0	33.0	40.0
46-47	37.759000228253264	40.0	37.0	40.0	33.0	40.0
48-49	37.741794091683154	40.0	37.0	40.0	33.0	40.0
50-51	37.6661399544321	40.0	37.0	40.0	33.0	40.0
52-53	37.538205644148235	40.0	37.0	40.0	33.0	40.0
54-55	37.37582137471147	40.0	37.0	40.0	33.0	40.0
56-57	37.283108225181415	40.0	37.0	40.0	33.0	40.0
58-59	37.1449893402386	37.0	37.0	40.0	33.0	40.0
60-61	36.993131533791924	37.0	37.0	40.0	33.0	40.0
62-63	36.79848459346768	37.0	37.0	40.0	33.0	40.0
64-65	36.61403736223306	37.0	37.0	40.0	33.0	40.0
66-67	36.41384635945143	37.0	37.0	40.0	33.0	40.0
68-69	36.132536647974625	37.0	37.0	40.0	33.0	40.0
70-71	35.904442976005384	37.0	37.0	37.0	33.0	40.0
72-73	35.58378023855401	37.0	33.0	37.0	33.0	40.0
74-75	35.49438185483382	37.0	33.0	37.0	33.0	40.0
76-77	33.816616802956474	35.0	33.0	37.0	27.0	37.0
78-79	35.06391306155561	37.0	33.0	37.0	33.0	37.0
80-81	35.12019174718881	37.0	33.0	37.0	33.0	37.0
82-83	34.971350620150204	37.0	33.0	37.0	33.0	37.0
84-85	34.84654662911038	37.0	33.0	37.0	33.0	37.0
86-87	34.74614757676807	37.0	33.0	37.0	33.0	37.0
88-89	34.63511883235956	37.0	33.0	37.0	33.0	37.0
90-91	34.54265379076477	37.0	33.0	37.0	33.0	37.0
92-93	34.511238718056504	37.0	33.0	37.0	33.0	37.0
94-95	34.52493878029202	37.0	33.0	37.0	33.0	37.0
96-97	34.51389808107277	37.0	33.0	37.0	33.0	37.0
98-99	34.51538830883239	37.0	33.0	37.0	33.0	37.0
100	34.509787364229915	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	3.0
19	18.0
20	85.0
21	340.0
22	1269.0
23	3716.0
24	8517.0
25	16849.0
26	29194.0
27	45739.0
28	66642.0
29	92548.0
30	124438.0
31	165772.0
32	217736.0
33	291265.0
34	400419.0
35	585926.0
36	1039919.0
37	2495411.0
38	2803729.0
39	88410.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18842630042701	19.7924119828081	14.221687658779613	23.797474057985273
2	14.848749921266307	20.72326245059829	41.722169497187174	22.705818130948227
3	18.47952322413943	25.890610768221457	30.04626356431145	25.583602443327663
4	12.651590373422996	17.71859599011364	38.44156355796557	31.188250078497788
5	13.60859104316069	37.53017535143536	35.17903982875097	13.682193776652976
6	30.40905190950733	37.410535523580826	18.524050518840294	13.65636204807155
7	26.93280896103844	32.477206153471606	22.76273050099635	17.827254384493603
8	24.61885225501554	36.69558640736801	20.76238749338578	17.923173844230668
9	25.840186215003598	16.831136613107283	20.99178078830924	36.336896383579884
10-11	24.573506838977032	26.586876340485855	28.465953974160524	20.373662846376593
12-13	25.362917282702423	24.20928668853796	28.67463350873188	21.753162520027736
14-15	22.4488136129014	25.494719173751808	26.51530932407311	25.541157889273684
16-17	21.868846534290263	28.558379588640925	27.151912739241318	22.42086113782749
18-19	21.900835414615756	27.652529280087418	29.035092934066814	21.411542371230013
20-21	22.781097108029627	26.755873278092313	28.999401075586857	21.463628538291204
22-23	22.491846365140695	26.68094640262426	28.849867834290183	21.97733939794486
24-25	22.218320638338454	26.858552618964733	28.942075107338294	21.98105163535852
26-27	22.11826489766297	26.950861146122566	28.918100808565494	22.012773147648968
28-29	22.074742293911	27.015874905278775	28.939750511054736	21.969632289755488
30-31	22.34084294481559	26.91432555063647	28.783654078612887	21.96117742593505
32-33	22.100393057708192	27.116414801905847	28.722356604723032	22.06083553566293
34-35	22.273113062386436	27.113356643859245	28.69914518633997	21.914385107414354
36-37	22.274145466491905	27.033165046283365	28.630420038584823	22.06226944863991
38-39	22.11890895432822	27.18327377980015	28.688121768387365	22.009695497484266
40-41	22.439092108756835	27.04340476652013	28.494668709976413	22.02283441474663
42-43	22.242263715861778	27.150539969305658	28.316565353393404	22.290630961439163
44-45	22.315865403695334	27.293077632756773	28.134463772049777	22.25659319149812
46-47	22.41242829837374	27.330302853422978	27.82202414754458	22.435244700658696
48-49	22.340313621951317	27.434520876288627	27.767015599986074	22.458149901773982
50-51	22.354328611921897	27.541588381353392	27.620492805719415	22.483590201005292
52-53	22.557011353006672	27.44518413905569	27.476554647571565	22.521249860366073
54-55	22.54750015156398	27.33662838745497	27.56667379283278	22.54919766814827
56-57	22.491465370965766	27.464260989711498	27.52595276428089	22.51832087504184
58-59	22.460495062677044	27.498309793189225	27.53060979445996	22.51058534967377
60-61	22.559831584419232	27.452912413791708	27.543699101226178	22.443556900562886
62-63	22.494609452014007	27.534209994581442	27.472419203070135	22.498761350334412
64-65	22.496283443196326	27.477047728098896	27.425391409752965	22.601277418951817
66-67	22.551690474167522	27.547869100534417	27.24871090250605	22.65172952279201
68-69	22.525495205625802	27.521518286650686	27.339584737622197	22.613401770101316
70-71	22.530351366919227	27.30409323616082	27.43221199843115	22.733343398488802
72-73	22.642761119676642	27.309907885806297	27.253373066972493	22.793957927544568
74-75	22.426198887739666	27.511574369357366	27.2797454217303	22.782481321172664
76-77	22.633686082003596	27.39117204576655	27.22862107745396	22.7465207947759
78-79	22.597810763408866	27.367007286151008	27.18376396178125	22.851417988658877
80-81	22.601512080835956	27.438568122691276	27.169354490966324	22.790565305506448
82-83	22.689982157861223	27.3503726388375	27.265252645440754	22.694392557860525
84-85	22.742496285846293	27.37312738094897	27.183587850815762	22.700788482388973
86-87	22.61823889379924	27.370542927894782	27.31195291885414	22.699265259451845
88-89	22.671762587089294	27.307485850079765	27.300480976280987	22.72027058654995
90-91	22.740200867649556	27.35264083313852	27.25339494350835	22.653763355703575
92-93	22.799609117221912	27.236103944309242	27.33404080089899	22.630246137569863
94-95	22.624457326957717	27.266338170413242	27.411724793370944	22.697479709258094
96-97	22.6871791544396	27.353613239205316	27.47531121280221	22.483896393552868
98-99	23.82547304187133	29.28130440594881	27.59905794753171	19.294164604648152
100	22.670484501144735	30.296427801906205	21.517558320322394	25.515529376626674
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1944.0
1	1707.5
2	2327.5
3	3437.0
4	4639.5
5	7504.0
6	10143.0
7	12384.0
8	14362.0
9	14735.0
10	13480.5
11	11903.5
12	12524.5
13	15912.5
14	24285.5
15	36372.0
16	46409.5
17	52283.0
18	53612.5
19	50723.0
20	45683.5
21	40520.5
22	37262.5
23	37802.5
24	42664.5
25	52060.5
26	64991.0
27	79530.0
28	94949.5
29	112158.0
30	129939.5
31	147260.0
32	166523.5
33	186304.5
34	205815.5
35	224356.5
36	243527.0
37	260619.0
38	273550.0
39	285831.0
40	297214.0
41	306709.5
42	312502.0
43	318362.5
44	325237.0
45	333406.0
46	338199.0
47	338541.0
48	339675.5
49	340225.0
50	339076.0
51	334340.0
52	325380.0
53	313447.5
54	300060.5
55	284063.5
56	265290.0
57	242447.5
58	217575.0
59	191624.0
60	162616.5
61	133294.5
62	106125.5
63	82964.5
64	62909.0
65	46829.0
66	35489.0
67	27098.5
68	20608.5
69	15162.0
70	10877.0
71	7725.5
72	5353.5
73	3731.5
74	2464.5
75	1592.5
76	1058.5
77	652.5
78	395.5
79	260.0
80	194.5
81	137.0
82	97.0
83	70.0
84	48.5
85	32.0
86	17.0
87	11.0
88	8.0
89	7.0
90	5.0
91	3.0
92	3.0
93	2.5
94	1.5
95	1.0
96	1.0
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	4.7181239418132646E-5
10-11	0.0016867293091982422
12-13	1.8282730274526401E-4
14-15	0.0012503028445805151
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	154.0
20-21	526.0
22-23	1116.0
24-25	3120.0
26-27	3276.0
28-29	7012.0
30-31	17021.0
32-33	21185.0
34-35	30261.0
36-37	43384.0
38-39	50192.0
40-41	53886.0
42-43	64196.0
44-45	77118.0
46-47	74623.0
48-49	67329.0
50-51	58782.0
52-53	55393.0
54-55	58725.0
56-57	62683.0
58-59	63566.0
60-61	61987.0
62-63	62570.0
64-65	66158.0
66-67	70568.0
68-69	72536.0
70-71	71845.0
72-73	68551.0
74-75	70038.0
76-77	69059.0
78-79	74634.0
80-81	76500.0
82-83	73624.0
84-85	74722.0
86-87	78371.0
88-89	84709.0
90-91	89181.0
92-93	93243.0
94-95	105579.0
96-97	283394.0
98-99	512787.0
100-101	5504342.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.55457375439978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.52321815151167	75.69597551923215
2	7.3209164848906925	12.380339457429827
3	1.7636769548057214	4.4738085946216675
4	0.6328318160146892	2.1403529784537914
5	0.2925626741754935	1.2368756105678098
6	0.14748241533990233	0.7482187659200876
7	0.09123642830117444	0.5400120114115763
8	0.057688802961441805	0.3902281715845014
9	0.036835328772381846	0.28031359711067333
>10	0.12896157007300982	1.7158816310848277
>50	0.003363292998073873	0.19423084611615557
>100	0.0011543002479132542	0.1489366524144263
>500	4.7853301521485464E-5	0.029759905465888376
>1k	2.3926606359992444E-5	0.025066258586743467
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1795309854533161E-5	0.0	0.0	0.0
3	0.0	1.1795309854533161E-5	0.0	1.1795309854533161E-5	0.0
4	0.0	1.1795309854533161E-5	0.0	2.3590619709066323E-5	0.0
5	0.0	1.1795309854533161E-5	0.0	2.3590619709066323E-5	0.0
6	4.7181239418132646E-5	1.1795309854533161E-5	0.0	2.3590619709066323E-5	0.0
7	4.7181239418132646E-5	1.1795309854533161E-5	0.0	3.538592956359948E-5	0.0
8	4.7181239418132646E-5	1.1795309854533161E-5	0.0	3.538592956359948E-5	0.0
9	4.7181239418132646E-5	3.538592956359948E-5	0.0	5.897654927266581E-5	1.1795309854533161E-5
10-11	5.307889434539923E-5	3.538592956359948E-5	0.0	1.533390281089311E-4	1.1795309854533161E-5
12-13	5.897654927266581E-5	3.538592956359948E-5	0.0	1.592366830361977E-4	1.1795309854533161E-5
14-15	5.897654927266581E-5	8.256716898173213E-5	0.0	2.0641792245433034E-4	1.1795309854533161E-5
16-17	8.256716898173213E-5	8.256716898173213E-5	0.0	2.2411088723613007E-4	1.1795309854533161E-5
18-19	8.256716898173213E-5	8.256716898173213E-5	0.0	2.477015069451964E-4	2.3590619709066323E-5
20-21	8.256716898173213E-5	8.256716898173213E-5	0.0	3.243710209996619E-4	2.3590619709066323E-5
22-23	8.256716898173213E-5	1.0026013376353187E-4	0.0	5.248912885267257E-4	2.94882746363329E-5
24-25	8.256716898173213E-5	1.1205544361806504E-4	0.0	0.0011559403657442498	3.538592956359948E-5
26-27	8.256716898173213E-5	1.1795309854533162E-4	0.0	0.0031847336607239533	3.538592956359948E-5
28-29	8.256716898173213E-5	1.1795309854533162E-4	0.0	0.011494529453242565	3.538592956359948E-5
30-31	8.256716898173213E-5	1.2974840839986477E-4	0.0	0.014372585057748657	3.538592956359948E-5
32-33	8.256716898173213E-5	1.2974840839986477E-4	0.0	0.020718461759487498	3.538592956359948E-5
34-35	8.256716898173213E-5	1.6513433796346426E-4	0.0	0.02741230010193507	3.538592956359948E-5
36-37	8.256716898173213E-5	2.0052026752706375E-4	0.0	0.037845251668269644	3.538592956359948E-5
38-39	8.256716898173213E-5	2.0052026752706375E-4	0.0	0.061943069701080905	3.538592956359948E-5
40-41	8.256716898173213E-5	2.123155773815969E-4	0.0	0.08674860632516414	3.538592956359948E-5
42-43	8.256716898173213E-5	2.2411088723613007E-4	0.0	0.10317357529760157	4.1283584490866064E-5
44-45	8.256716898173213E-5	2.6539447172699613E-4	0.0	0.12310175129683534	4.7181239418132646E-5
46-47	8.256716898173213E-5	2.9488274636332905E-4	0.0	0.14322454990866892	5.897654927266581E-5
48-49	8.256716898173213E-5	2.9488274636332905E-4	0.0	0.1623211565631581	7.077185912719897E-5
50-51	8.256716898173213E-5	3.302686759269285E-4	0.0	0.1794715370916493	7.077185912719897E-5
52-53	8.256716898173213E-5	3.302686759269285E-4	0.0	0.2172578122106463	7.077185912719897E-5
54-55	8.256716898173213E-5	3.302686759269285E-4	0.0	0.2616199725735455	7.077185912719897E-5
56-57	8.256716898173213E-5	3.302686759269285E-4	0.0	0.3071026873726254	7.666951405446555E-5
58-59	8.256716898173213E-5	3.302686759269285E-4	0.0	0.339858262838664	8.256716898173213E-5
60-61	9.436247883626529E-5	3.4206398578146165E-4	0.0	0.36545408522300094	8.256716898173213E-5
62-63	1.1795309854533162E-4	3.6565460549052803E-4	0.0	0.38389015452563624	1.0615778869079845E-4
64-65	1.238507534725982E-4	4.010405350541275E-4	0.0	0.3890977838264127	1.0615778869079845E-4
66-67	1.3564606332713136E-4	4.128358449086607E-4	0.0	0.39483030441571576	1.0615778869079845E-4
68-69	1.4154371825439793E-4	4.128358449086607E-4	0.0	0.40081642416689134	1.0615778869079845E-4
70-71	1.474413731816645E-4	4.187334998359272E-4	0.0	0.40582943085506795	1.0615778869079845E-4
72-73	1.6513433796346426E-4	4.246311547631938E-4	0.0	0.40992830102951827	1.0615778869079845E-4
74-75	1.6513433796346426E-4	4.246311547631938E-4	0.0	0.4130776487606786	1.0615778869079845E-4
76-77	1.7103199289073082E-4	4.305288096904604E-4	0.0	0.41616801994256625	1.0615778869079845E-4
78-79	1.7692964781799742E-4	4.36426464617727E-4	0.0	0.41823219916710963	1.0615778869079845E-4
80-81	1.7692964781799742E-4	4.36426464617727E-4	0.0	0.41899299665272693	1.0615778869079845E-4
82-83	1.7692964781799742E-4	4.36426464617727E-4	0.0	0.41938224187792655	1.0615778869079845E-4
84-85	1.8282730274526401E-4	4.36426464617727E-4	0.0	0.4195650691806718	1.0615778869079845E-4
86-87	1.9462261259979715E-4	4.4822177447226014E-4	0.0	0.4196181480750172	1.0615778869079845E-4
88	2.0052026752706375E-4	4.600170843267933E-4	0.0	0.41962994338487175	1.0615778869079845E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11485	0.0	52.549004	1
GTATCAA	21635	0.0	37.962696	1
TCAACGC	26540	0.0	30.288166	4
ATCAACG	26980	0.0	29.857407	3
CAACGCA	27115	0.0	29.679066	5
TATCAAC	27550	0.0	29.440788	2
AACGCAG	27975	0.0	28.751442	6
ACGCAGA	32085	0.0	25.108318	7
GTGGTAT	5330	0.0	24.78944	1
CGCAGAG	32895	0.0	24.451326	8
TGGTATC	5245	0.0	23.24089	2
GTACATG	28225	0.0	22.545406	1
TACATGG	28275	0.0	21.902576	2
GCAGAGT	37470	0.0	21.295618	9
ACATGGG	28485	0.0	21.277256	3
GAGTACT	22475	0.0	19.827448	12-13
CATGGGG	20960	0.0	19.135094	4
CAGAGTA	36725	0.0	18.947828	10-11
AGAGTAC	34310	0.0	18.927351	10-11
GTACTTT	25035	0.0	17.851664	14-15
>>END_MODULE