FastQCFastQC Report
Fri 27 May 2016
SRR2139554_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139554_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9837921
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134750.051.62491
GTATCAA244450.037.602181
TCAACGC298850.030.0903174
ATCAACG302650.029.712513
CAACGCA305700.029.3750385
TATCAAC311500.029.0453762
AACGCAG312650.028.762756
CTTATAC122150.028.648681
ACGCAGA358950.025.0409037
CGCAGAG367550.024.4897388
TATACAC151100.024.4229973
GTGGTAT61050.022.511451
TTATACA163800.022.1668512
GTACATG295500.021.7900621
TACATGG299250.021.205162
GCAGAGT422650.021.1970239
TGGTATC59500.021.1730612
ACATGGG302950.020.3161133
GAGTACT261800.020.06685612-13
CAGAGTA414550.019.02235610-11
Produced by FastQC (version 0.11.5)