FastQCFastQC Report
Fri 27 May 2016
SRR2139553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9671205
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134900.054.4683571
GTATCAA253450.039.0491681
TCAACGC311800.031.0683944
ATCAACG316850.030.586743
TATCAAC321300.030.3496462
CAACGCA319650.030.2659845
AACGCAG329850.029.395016
AGACCGA66100.026.11317688-89
CGAGACC67450.025.45670586-87
ACCGAGG70250.025.25938290-91
GTGGTAT61950.025.2431561
ACGCAGA383950.025.2309557
GAGACCG66850.024.98052888-89
GACCGAG68550.024.80026690-91
CGCAGAG394750.024.5630028
TGGTATC60550.023.8455752
CACGAGA73950.023.37975584-85
TCTCCGA71750.023.0879374-75
ACGAGAC74400.022.8557186-87
TCCGAGC74150.022.576476-77
Produced by FastQC (version 0.11.5)