##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139553_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9671205
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59928075146789	33.0	33.0	33.0	33.0	33.0
2	32.5985806318861	33.0	33.0	33.0	33.0	33.0
3	32.595351354872534	33.0	33.0	33.0	33.0	33.0
4	36.501705423471016	37.0	37.0	37.0	37.0	37.0
5	36.51975064120759	37.0	37.0	37.0	37.0	37.0
6	36.51486645149183	37.0	37.0	37.0	37.0	37.0
7	36.48419281775125	37.0	37.0	37.0	37.0	37.0
8	36.485313464040935	37.0	37.0	37.0	37.0	37.0
9	36.52080335387369	37.0	37.0	37.0	37.0	37.0
10-11	36.45352590499323	37.0	37.0	37.0	37.0	37.0
12-13	36.449568693870106	37.0	37.0	37.0	37.0	37.0
14-15	38.46111399768695	40.0	37.0	40.0	37.0	40.0
16-17	38.39447493874859	40.0	37.0	40.0	37.0	40.0
18-19	38.410139636167365	40.0	37.0	40.0	37.0	40.0
20-21	38.4072867342489	40.0	37.0	40.0	37.0	40.0
22-23	38.41196443015099	40.0	37.0	40.0	37.0	40.0
24-25	38.38890722580275	40.0	37.0	40.0	37.0	40.0
26-27	38.270621199367156	40.0	37.0	40.0	37.0	40.0
28-29	38.219880081021856	40.0	37.0	40.0	37.0	40.0
30-31	38.15364151767372	40.0	37.0	40.0	37.0	40.0
32-33	38.080538269383325	40.0	37.0	40.0	33.0	40.0
34-35	38.03639425585153	40.0	37.0	40.0	33.0	40.0
36-37	37.99233115154574	40.0	37.0	40.0	33.0	40.0
38-39	37.932484413909535	40.0	37.0	40.0	33.0	40.0
40-41	37.87756691105365	40.0	37.0	40.0	33.0	40.0
42-43	37.813900241715146	40.0	37.0	40.0	33.0	40.0
44-45	37.764969561600694	40.0	37.0	40.0	33.0	40.0
46-47	37.69860930193153	40.0	37.0	40.0	33.0	40.0
48-49	37.681116068163846	40.0	37.0	40.0	33.0	40.0
50-51	37.604065510640254	40.0	37.0	40.0	33.0	40.0
52-53	37.474957773550464	40.0	37.0	40.0	33.0	40.0
54-55	37.30904772159052	40.0	37.0	40.0	33.0	40.0
56-57	37.21508620697089	38.5	37.0	40.0	33.0	40.0
58-59	37.074680489891044	37.0	37.0	40.0	33.0	40.0
60-61	36.92481318146466	37.0	37.0	40.0	33.0	40.0
62-63	36.724173203918944	37.0	37.0	40.0	33.0	40.0
64-65	36.54089079101338	37.0	37.0	40.0	33.0	40.0
66-67	36.33518941108896	37.0	37.0	40.0	33.0	40.0
68-69	36.0522539212827	37.0	37.0	40.0	33.0	40.0
70-71	35.82033802193922	37.0	35.0	37.0	33.0	40.0
72-73	35.49904155501364	37.0	33.0	37.0	33.0	40.0
74-75	35.4081148415795	37.0	33.0	37.0	33.0	40.0
76-77	33.726527633997776	35.0	33.0	37.0	27.0	37.0
78-79	34.981859045280714	37.0	33.0	37.0	33.0	37.0
80-81	35.03512011965222	37.0	33.0	37.0	33.0	37.0
82-83	34.887234287132785	37.0	33.0	37.0	33.0	37.0
84-85	34.762759947229036	37.0	33.0	37.0	33.0	37.0
86-87	34.66289284034008	37.0	33.0	37.0	33.0	37.0
88-89	34.54756928347197	37.0	33.0	37.0	33.0	37.0
90-91	34.452395405133736	37.0	33.0	37.0	33.0	37.0
92-93	34.41809186722906	37.0	33.0	37.0	33.0	37.0
94-95	34.424004978964916	37.0	33.0	37.0	33.0	37.0
96-97	34.41602881933292	37.0	33.0	37.0	33.0	37.0
98-99	34.42934268321925	37.0	33.0	37.0	33.0	37.0
100	34.42562811596298	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	13.0
20	87.0
21	379.0
22	1538.0
23	4421.0
24	10484.0
25	20152.0
26	35109.0
27	54943.0
28	80262.0
29	111625.0
30	149767.0
31	197049.0
32	258993.0
33	344780.0
34	473546.0
35	691460.0
36	1223764.0
37	2883834.0
38	3043396.0
39	85600.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18125869527117	19.97997147201409	14.267529227226596	23.571240605488146
2	14.924562140912121	20.90489240999441	41.470261461730985	22.700283987362486
3	18.419142185487743	26.10649862142308	30.08798800149516	25.386371191594016
4	12.682432023724036	17.873046843697345	38.50932743127666	30.935193701301955
5	13.5933526380632	37.60437298144337	35.14061587982056	13.661658500672875
6	30.185080349346332	37.44535453441428	18.658967522661342	13.71059759357805
7	26.851162807530187	32.56100971905776	22.80238088221685	17.785446591195203
8	24.697511840561752	36.388464519157644	20.87522702703541	18.038796613245196
9	25.888510100618433	16.849963654938016	21.056995054383172	36.20453119006037
10-11	24.442534390027138	26.691308953685976	28.460409397329865	20.40574725895702
12-13	25.33970766554619	24.319046594403186	28.64521331051227	21.696032429538352
14-15	22.4965912469664	25.537059878192238	26.463876730763236	25.502472144078126
16-17	21.830723265611677	28.6434989228333	27.14747541800634	22.378302393548687
18-19	21.907911165154704	27.703445434152208	29.01076959903135	21.377873801661735
20-21	22.802788928867713	26.81595081684297	28.960455086484238	21.420805167805078
22-23	22.5172645824076	26.689072887710747	28.849222939969067	21.944439589912587
24-25	22.19804925555261	26.866476068607852	28.919175170041644	22.016299505797893
26-27	22.085487825607718	26.98780848523799	28.925705722849465	22.000997966304826
28-29	22.092546418631418	26.934941981238996	28.96636971576966	22.00614188435993
30-31	22.312502590244105	26.938155331758466	28.774995855609433	21.974346222387997
32-33	22.09633086739576	27.1304209347703	28.7097432070705	22.06350499076344
34-35	22.2387881207641	27.087063826350295	28.70771801448746	21.966430038398144
36-37	22.260059194700315	27.005428748943277	28.636988878990895	22.09752317736551
38-39	22.10165497627038	27.183185903469802	28.735854473503807	21.97930464675601
40-41	22.378664081216556	27.05170782308962	28.525658302758792	22.043969792935034
42-43	22.219693005115342	27.148648306144512	28.345446211017578	22.286212477722568
44-45	22.31564143034111	27.230873790879535	28.1882138838441	22.265270894935256
46-47	22.4422	27.262135135135136	27.843281081081084	22.452383783783784
48-49	22.357493127149827	27.38141654603438	27.802768858580645	22.458321468235145
50-51	22.382529057514635	27.52270428775075	27.62419543151351	22.470571223221103
52-53	22.558549655154096	27.410141966728347	27.481130864372417	22.550177513745144
54-55	22.55564056464306	27.311676182002337	27.57398993773168	22.55869331562292
56-57	22.494526765470603	27.462369145294417	27.559160453660507	22.483943635574466
58-59	22.405432594437563	27.38878457611063	27.52532768200196	22.680455147449845
60-61	22.575576741499372	27.371872514890132	27.620260639643597	22.4322901039669
62-63	22.414143615731554	27.454463448494188	27.584453347776783	22.546939587997475
64-65	22.418676194823846	27.34370291656651	27.560265063061927	22.677355825547718
66-67	22.4797417783143	27.420791909417208	27.551210607767906	22.54825570450059
68-69	22.444605006236756	27.578462527980776	27.44974274850758	22.527189717274897
70-71	22.41969225759334	27.368734541140828	27.382014236688086	22.82955896457775
72-73	22.55241825201373	27.356804228800797	27.20044287209888	22.890334647086597
74-75	22.333183100260477	27.591249966371368	27.39716818214657	22.678398751221586
76-77	22.557805497742613	27.26046292494546	27.33529303587408	22.846438541437845
78-79	22.459449616420745	27.254993069619555	27.090869186010497	23.194688127949203
80-81	22.678337238699957	27.492753382185757	27.071231632616392	22.757677746497894
82-83	22.73432667621182	27.243608525729705	27.189760473414704	22.832304324643772
84-85	22.562604733970705	27.21920260761391	27.142866501557982	23.075326156857404
86-87	22.478168390719276	27.417396991009912	27.252506993438413	22.851927624832403
88-89	22.765584718758404	27.370347522185963	27.212306898056248	22.65176086099939
90-91	22.80277331685778	27.38221090026048	27.188261645208673	22.626754137673068
92-93	22.64761130994301	27.10113781241217	27.24669727692326	23.004553600721568
94-95	22.674744115363286	27.312063736543617	27.33058628508635	22.682605863006742
96-97	23.020010437776318	27.194367609469307	27.352869479622733	22.432752473131643
98-99	23.77730572337026	29.209947452494717	27.669794662857438	19.342952161277584
100	22.70647609026552	30.194255250724066	21.517340826109503	25.58192783290091
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2663.0
1	2257.5
2	2824.0
3	4031.0
4	5216.5
5	8238.0
6	11013.5
7	13591.5
8	16170.0
9	16765.0
10	15176.0
11	13191.0
12	13852.0
13	18047.0
14	28342.0
15	42443.5
16	53799.0
17	60570.5
18	61776.5
19	57731.5
20	51242.0
21	44277.0
22	39472.5
23	39471.0
24	44853.5
25	55674.0
26	71044.5
27	87852.5
28	104220.5
29	123327.5
30	142925.5
31	162122.5
32	185517.0
33	209490.5
34	231433.0
35	252546.5
36	276788.0
37	297600.0
38	311997.0
39	325466.5
40	338383.0
41	350407.5
42	357521.0
43	366097.5
44	376825.5
45	384303.5
46	387887.0
47	387133.0
48	387595.5
49	390012.5
50	388891.5
51	384061.0
52	376320.0
53	360988.0
54	340031.5
55	320327.0
56	297691.5
57	270461.0
58	243620.0
59	215273.5
60	182811.5
61	149048.0
62	118014.0
63	91036.5
64	67558.5
65	49024.5
66	36174.5
67	27374.5
68	20870.5
69	15058.5
70	10425.5
71	7242.0
72	4745.5
73	2999.0
74	1857.5
75	1118.5
76	739.5
77	502.0
78	344.5
79	242.0
80	170.5
81	122.5
82	98.0
83	78.5
84	60.0
85	41.0
86	26.5
87	18.5
88	13.0
89	7.5
90	3.0
91	2.0
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5509959720634605E-4
10-11	0.001654395703534358
12-13	2.274794092359742E-4
14-15	0.0015148060660486466
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	147.0
20-21	562.0
22-23	1254.0
24-25	2727.0
26-27	3448.0
28-29	7420.0
30-31	17821.0
32-33	22874.0
34-35	31775.0
36-37	45877.0
38-39	52675.0
40-41	58145.0
42-43	70067.0
44-45	85126.0
46-47	83301.0
48-49	73954.0
50-51	63495.0
52-53	59488.0
54-55	63513.0
56-57	68076.0
58-59	41050.0
60-61	70423.0
62-63	68451.0
64-65	73592.0
66-67	78328.0
68-69	80927.0
70-71	80137.0
72-73	76749.0
74-75	78852.0
76-77	77766.0
78-79	83879.0
80-81	84897.0
82-83	82449.0
84-85	83842.0
86-87	88478.0
88-89	96377.0
90-91	101603.0
92-93	106264.0
94-95	120967.0
96-97	355107.0
98-99	592196.0
100-101	6337126.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.61327920403903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.4997599474097	75.72868177124647
2	7.346156045973351	12.431647051887653
3	1.7574521935601348	4.461113794243636
4	0.6348220890715375	2.148575146700053
5	0.29163391794788857	1.2338051062346254
6	0.1531083620099561	0.7773000349932899
7	0.08967406233077044	0.5311331531347715
8	0.06139147818189432	0.41556274273226357
9	0.037583802880387734	0.28620799260010327
>10	0.124231287237173	1.6001200988967155
>50	0.0030978809143679077	0.17215442190066982
>100	9.641740517740138E-4	0.11987239350570583
>500	8.317330775167622E-5	0.04428364841161891
>1k	4.158512343341829E-5	0.049542643512504994
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.0679946294179473E-5	0.0	1.0339973147089737E-5	0.0
3	0.0	2.0679946294179473E-5	0.0	1.0339973147089737E-5	0.0
4	0.0	4.1359892588358946E-5	0.0	3.101991944126921E-5	0.0
5	0.0	5.169986573544868E-5	0.0	3.101991944126921E-5	0.0
6	8.271978517671789E-5	5.169986573544868E-5	0.0	3.101991944126921E-5	1.0339973147089737E-5
7	8.271978517671789E-5	5.169986573544868E-5	0.0	4.1359892588358946E-5	1.0339973147089737E-5
8	8.271978517671789E-5	5.169986573544868E-5	0.0	4.1359892588358946E-5	3.101991944126921E-5
9	8.271978517671789E-5	1.2407967776507684E-4	0.0	5.169986573544868E-5	3.101991944126921E-5
10-11	8.788977175026276E-5	1.2407967776507684E-4	0.0	8.788977175026276E-5	3.101991944126921E-5
12-13	9.305975832380763E-5	1.2407967776507684E-4	0.0	1.0339973147089737E-4	3.101991944126921E-5
14-15	1.0339973147089737E-4	1.7577954350052552E-4	0.0	1.1890969119153197E-4	5.169986573544868E-5
16-17	1.7577954350052552E-4	1.8611951664761526E-4	0.0	1.447596240592563E-4	6.203983888253842E-5
18-19	1.8611951664761526E-4	2.2230942266242932E-4	0.0	2.0162947636824985E-4	7.754979860317302E-5
20-21	1.9128950322116014E-4	2.6883930182433315E-4	0.0	2.481593555301537E-4	9.305975832380763E-5
22-23	2.0679946294179473E-4	3.050292078391472E-4	0.0	4.0842893931004464E-4	1.0339973147089737E-4
24-25	2.0679946294179473E-4	3.15369180986237E-4	0.0	0.0010805271938708776	1.2407967776507684E-4
26-27	2.0679946294179473E-4	3.2053916755978186E-4	0.0	0.0029158724274793058	1.2407967776507684E-4
28-29	2.0679946294179473E-4	3.2053916755978186E-4	0.0	0.009988414060088685	1.3441965091216658E-4
30-31	2.0679946294179473E-4	3.618990601481408E-4	0.0	0.01158593991131405	1.602695837798909E-4
32-33	2.0679946294179473E-4	3.670690467216857E-4	0.0	0.01509119080817747	1.8094953007407038E-4
34-35	2.0679946294179473E-4	5.014886976338522E-4	0.0	0.019537379261426055	1.8611951664761526E-4
36-37	2.119694495153396E-4	6.30738361972474E-4	0.0	0.02666162075977089	2.0679946294179473E-4
38-39	2.1713943608888447E-4	6.410783351195637E-4	0.0	0.045842270947622346	2.119694495153396E-4
40-41	2.1713943608888447E-4	7.341380934433714E-4	0.0	0.06629473783256584	2.1713943608888447E-4
42-43	2.274794092359742E-4	7.599880263110957E-4	0.0	0.07826325675032222	2.1713943608888447E-4
44-45	2.326493958095191E-4	8.685577443555379E-4	0.0	0.09388695617557481	2.1713943608888447E-4
46-47	2.3781938238306394E-4	9.616175026793455E-4	0.0	0.10963473527859248	2.5332934210369856E-4
48-49	2.429893689566088E-4	9.667874892528904E-4	0.0	0.12533598450244826	2.895192481185126E-4
50-51	2.998592212656024E-4	0.0010960371535915121	0.0	0.14064948473328814	2.895192481185126E-4
52-53	3.3087914070687157E-4	0.0011063771267386018	0.0	0.1770203402781763	2.998592212656024E-4
54-55	3.3087914070687157E-4	0.0011270570730327813	0.0	0.22278506142719548	3.15369180986237E-4
56-57	3.3087914070687157E-4	0.0011580769924740505	0.0	0.26844121285817024	3.2053916755978186E-4
58-59	3.3087914070687157E-4	0.001183926925341775	0.0	0.454157470553049	3.2053916755978186E-4
60-61	3.515590870010511E-4	0.0012304568045036785	0.0	0.6186819532829673	3.2053916755978186E-4
62-63	4.0325895273649975E-4	0.0012666467105184927	0.0	0.6257234749961353	3.2053916755978186E-4
64-65	4.0325895273649975E-4	0.0014424262540190182	0.0	0.6313432504015788	3.2053916755978186E-4
66-67	4.0842893931004464E-4	0.001463106200313198	0.0	0.6367562263440802	3.3087914070687157E-4
68-69	4.1359892588358946E-4	0.0015303160257692811	0.0	0.6422467520851849	3.4121911385396133E-4
70-71	4.1359892588358946E-4	0.0015613359452105503	0.0	0.6471323893971848	3.4638910042750616E-4
72-73	4.3427887217776894E-4	0.001576845904931185	0.0	0.6507307000523719	3.722390332952305E-4
74-75	4.3427887217776894E-4	0.0016233757840930887	0.0	0.6540136415265729	3.722390332952305E-4
76-77	4.394488587513138E-4	0.0017216055289904413	0.0	0.6570742735781114	3.722390332952305E-4
78-79	4.446188453248587E-4	0.0017577954350052553	0.0	0.6591784581135443	3.722390332952305E-4
80-81	4.446188453248587E-4	0.0017733053947258898	0.0	0.6599177661935612	3.9291897958941E-4
82-83	4.446188453248587E-4	0.0017836453678729796	0.0	0.6605019746763718	4.239388990306792E-4
84-85	4.446188453248587E-4	0.001809495300740704	0.0	0.6607139441258871	4.34278872177769E-4
86-87	4.549588184719484E-4	0.0018405152201819732	0.0	0.6607759839647696	4.549588184719484E-4
88	4.652987916190382E-4	0.001923235005358691	0.0	0.6607863239379167	4.549588184719484E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13490	0.0	54.468357	1
GTATCAA	25345	0.0	39.049168	1
TCAACGC	31180	0.0	31.068394	4
ATCAACG	31685	0.0	30.58674	3
TATCAAC	32130	0.0	30.349646	2
CAACGCA	31965	0.0	30.265984	5
AACGCAG	32985	0.0	29.39501	6
AGACCGA	6610	0.0	26.113176	88-89
CGAGACC	6745	0.0	25.456705	86-87
ACCGAGG	7025	0.0	25.259382	90-91
GTGGTAT	6195	0.0	25.243156	1
ACGCAGA	38395	0.0	25.230955	7
GAGACCG	6685	0.0	24.980528	88-89
GACCGAG	6855	0.0	24.800266	90-91
CGCAGAG	39475	0.0	24.563002	8
TGGTATC	6055	0.0	23.845575	2
CACGAGA	7395	0.0	23.379755	84-85
TCTCCGA	7175	0.0	23.08793	74-75
ACGAGAC	7440	0.0	22.85571	86-87
TCCGAGC	7415	0.0	22.5764	76-77
>>END_MODULE