FastQCFastQC Report
Fri 27 May 2016
SRR2139552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10546074
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136950.053.4257051
ATCTCGT87750.043.11948894
GTATCAA252950.038.6466331
TATCTCG110600.033.2688694
TCAACGC312200.030.6903694
ATCAACG316400.030.3375153
CAACGCA319400.030.0265485
TATCAAC324050.029.8077012
TATGCCG44250.029.12447586-87
AACGCAG331150.029.0914156
GCCGTCT49900.027.18757690-91
CCGTCTT49600.026.60657590-91
ATGCCGT50050.026.52064388-89
TCGTATG50200.026.46789684-85
CTCGTAT50000.025.8072182-83
TGCCGTC52000.025.31161788-89
TCTCGTA52650.025.22013382-83
ACGCAGA384000.024.9753257
CGCTATC176400.024.8831894
CGTATGC53150.024.43764184-85
Produced by FastQC (version 0.11.5)