##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139552_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10546074
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64675897400303	33.0	33.0	33.0	33.0	33.0
2	32.6432979704106	33.0	33.0	33.0	33.0	33.0
3	32.642508671947496	33.0	33.0	33.0	33.0	33.0
4	36.55653402394104	37.0	37.0	37.0	37.0	37.0
5	36.57403826295928	37.0	37.0	37.0	37.0	37.0
6	36.56436205549098	37.0	37.0	37.0	37.0	37.0
7	36.53446391519726	37.0	37.0	37.0	37.0	37.0
8	36.534205430381014	37.0	37.0	37.0	37.0	37.0
9	36.565981899994256	37.0	37.0	37.0	37.0	37.0
10-11	36.50222604165303	37.0	37.0	37.0	37.0	37.0
12-13	36.50078707014572	37.0	37.0	37.0	37.0	37.0
14-15	38.5763051254903	40.0	37.0	40.0	37.0	40.0
16-17	38.51651913309161	40.0	37.0	40.0	37.0	40.0
18-19	38.525629395356034	40.0	37.0	40.0	37.0	40.0
20-21	38.52357411730948	40.0	37.0	40.0	37.0	40.0
22-23	38.5291514039899	40.0	37.0	40.0	37.0	40.0
24-25	38.50780664302411	40.0	37.0	40.0	37.0	40.0
26-27	38.39489844469911	40.0	37.0	40.0	37.0	40.0
28-29	38.35022483079994	40.0	37.0	40.0	37.0	40.0
30-31	38.28859974025427	40.0	37.0	40.0	37.0	40.0
32-33	38.22109408198081	40.0	37.0	40.0	37.0	40.0
34-35	38.17966048091868	40.0	37.0	40.0	37.0	40.0
36-37	38.13818410022133	40.0	37.0	40.0	37.0	40.0
38-39	38.07905433077297	40.0	37.0	40.0	33.0	40.0
40-41	38.02897723251666	40.0	37.0	40.0	33.0	40.0
42-43	37.96749223153813	40.0	37.0	40.0	33.0	40.0
44-45	37.921430200167904	40.0	37.0	40.0	33.0	40.0
46-47	37.85783151684842	40.0	37.0	40.0	33.0	40.0
48-49	37.839151016311746	40.0	37.0	40.0	33.0	40.0
50-51	37.76206354059741	40.0	37.0	40.0	33.0	40.0
52-53	37.63855091909845	40.0	37.0	40.0	33.0	40.0
54-55	37.48121112582439	40.0	37.0	40.0	33.0	40.0
56-57	37.392151720831905	40.0	37.0	40.0	33.0	40.0
58-59	37.25692095435473	37.0	37.0	40.0	33.0	40.0
60-61	37.10436841510864	37.0	37.0	40.0	33.0	40.0
62-63	36.916120143672515	37.0	37.0	40.0	33.0	40.0
64-65	36.73826097715818	37.0	37.0	40.0	33.0	40.0
66-67	36.53026935114492	37.0	37.0	40.0	33.0	40.0
68-69	36.2431421704148	37.0	37.0	40.0	33.0	40.0
70-71	36.01975773197793	37.0	37.0	37.0	33.0	40.0
72-73	35.7042568862676	37.0	33.0	37.0	33.0	40.0
74-75	35.598577609099934	37.0	33.0	37.0	33.0	40.0
76-77	33.89752735824414	35.0	33.0	37.0	27.0	37.0
78-79	35.12372410434233	37.0	33.0	37.0	33.0	37.0
80-81	35.1655256644033	37.0	33.0	37.0	33.0	37.0
82-83	35.01424006678265	37.0	33.0	37.0	33.0	37.0
84-85	34.89693856106531	37.0	33.0	37.0	33.0	37.0
86-87	34.79651482474523	37.0	33.0	37.0	33.0	37.0
88-89	34.68701055618371	37.0	33.0	37.0	33.0	37.0
90-91	34.60774582409412	37.0	33.0	37.0	33.0	37.0
92-93	34.59025808546201	37.0	33.0	37.0	33.0	37.0
94-95	34.58149214873775	37.0	33.0	37.0	33.0	37.0
96-97	34.56573107328285	37.0	33.0	37.0	33.0	37.0
98-99	34.5715709145189	37.0	33.0	37.0	33.0	37.0
100	34.55810340476014	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	10.0
20	84.0
21	396.0
22	1604.0
23	4397.0
24	10238.0
25	20121.0
26	35053.0
27	54675.0
28	80094.0
29	111503.0
30	149288.0
31	198005.0
32	259361.0
33	346507.0
34	476327.0
35	700161.0
36	1249434.0
37	3102193.0
38	3635134.0
39	111488.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.00301458154001	19.97889451562733	14.204385442393065	23.81370546043959
2	14.747962132638174	20.88438787742244	41.538642721452554	22.82900726848683
3	18.2869663156166	26.171350589802422	30.14875488262267	25.39292821195831
4	12.628301299611591	17.856720899170632	38.732944600995594	30.782033200222187
5	13.580655701827999	37.659341286624766	35.2045889304399	13.555414081107338
6	30.105705687253852	37.47283586290026	18.794520121895598	13.626938327950288
7	26.684508377240668	32.614430735077335	23.008002788525854	17.693058099156143
8	24.641330982505906	36.42577323087245	21.02050488172186	17.912390904899773
9	25.857936355769574	16.88058537869396	21.204682847493217	36.056795418043244
10-11	24.486265291473185	26.643864777909158	28.574641733145832	20.295228197471815
12-13	25.29470967206037	24.22602115653272	28.737399200478436	21.74186997092847
14-15	22.412437702889203	25.53510549491844	26.531551092034565	25.520905710157788
16-17	21.891288644475658	28.59845284605437	27.136847323468434	22.37341118600154
18-19	21.92040848566016	27.774748214359203	28.88014060967143	21.424702690309207
20-21	22.83300008780765	26.905990245575058	28.7644828100876	21.496526856529695
22-23	22.562602904629873	26.791951186529143	28.621716653589324	22.023729255251656
24-25	22.22548978878336	26.985091739421	28.713435266234118	22.07598320556152
26-27	22.14849334041963	27.135902481220686	28.64958083523264	22.066023343127043
28-29	22.135059157583257	27.184000692587695	28.65796609699683	22.022974052832215
30-31	22.40554356519042	27.064639830379623	28.515349420103874	22.01446718432608
32-33	22.16592725664161	27.235268936918338	28.473188961226715	22.125614845213338
34-35	22.320164261275647	27.228648581115365	28.455643149071864	21.995544008537127
36-37	22.31324672770409	27.143198906651616	28.405353412175337	22.138200953468957
38-39	22.134664839599797	27.286069288960057	28.456701258683502	22.122564612756644
40-41	22.510779186033535	27.09409242511406	28.367926970140918	22.02720141871149
42-43	22.203145742830966	27.198214207806938	28.192535930868136	22.406104118493957
44-45	22.323807807415168	27.287184873887714	28.041817845040807	22.34718947365631
46-47	22.383194552155864	27.25778032240068	27.894033461923307	22.46499166352015
48-49	22.315809862035326	27.495215608562045	27.729786586433843	22.459187942968782
50-51	22.282453524123046	27.639951512718948	27.500603975336446	22.57699098782156
52-53	22.3749093927053	27.45342426361807	27.361018463377075	22.810647880299552
54-55	22.510249272504996	27.163961247208285	27.685747451288893	22.640042028997822
56-57	22.28873949329596	27.02441795671577	27.810609324391073	22.876233225597197
58-59	22.169093523218944	26.850248489195224	27.831565585866347	23.149092401719486
60-61	22.36141482132618	26.99696987917185	28.11908335042368	22.52253194907829
62-63	21.969593451816138	27.24163212391217	28.038031611318452	22.750742812953238
64-65	21.79704465177189	27.52338561455925	27.743904924681996	22.935664808986864
66-67	21.903930891786963	28.090164837897774	27.214037162887262	22.791867107428
68-69	21.967920053644757	28.099209988848706	27.04502910079933	22.887840856707207
70-71	21.9250585905759	27.60021340655017	27.059843644419356	23.414884358454575
72-73	22.227182032730106	27.12062538099524	26.994733296717836	23.657459289556815
74-75	22.20373927059479	27.247102822772657	27.182192009858895	23.366965896773653
76-77	22.538637879671764	27.137307457560205	26.613678465997925	23.71037619677011
78-79	22.71318499050765	27.197687061761172	26.157029488894644	23.93209845883654
80-81	22.57650532506997	27.53886397532731	26.34177043381533	23.54286026578739
82-83	22.557551677766885	27.308520880168523	26.45797631050178	23.675951131562815
84-85	22.65345375572155	27.68029870642799	26.353448853977383	23.312798683873073
86-87	22.98067007344159	27.619687431073704	26.44070345323517	22.95893904224954
88-89	22.794002775828478	27.718120233179	26.718433917672375	22.76944307332015
90-91	22.777277975266774	27.38397608333311	26.62720857369268	23.21153736770744
92-93	22.760188739212282	26.938547475916373	26.892180627277483	23.409083157593862
94-95	22.04488444786012	27.39965486127937	27.449865097503896	23.105595593356618
96-97	22.190584968080984	27.27369960827032	27.5990435353357	22.936671888312997
98-99	23.426085780078285	29.135079749819287	27.890729562493032	19.548104907609396
100	22.38542341989788	30.27309243390774	21.589904122857504	25.751580023336874
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3269.0
1	2770.5
2	3254.5
3	4486.0
4	5810.0
5	9020.5
6	11790.5
7	13845.5
8	15517.5
9	15685.0
10	14579.5
11	13256.0
12	14206.5
13	18125.0
14	27364.0
15	40356.5
16	51407.0
17	58462.0
18	60195.0
19	56801.0
20	50889.5
21	44809.0
22	41203.0
23	42302.0
24	48352.5
25	58731.5
26	73569.5
27	91407.0
28	109991.5
29	131684.5
30	153151.5
31	173665.0
32	196870.0
33	220106.5
34	243071.0
35	265108.5
36	290974.5
37	315908.5
38	331965.5
39	345720.0
40	362488.0
41	377564.5
42	388509.0
43	400680.0
44	412283.0
45	423256.0
46	430939.0
47	433621.0
48	435802.5
49	435624.0
50	431549.0
51	424511.0
52	413828.0
53	398347.5
54	378329.0
55	353883.0
56	326410.5
57	294279.5
58	260237.5
59	227543.5
60	191423.0
61	154686.5
62	121560.5
63	92681.0
64	68185.0
65	49601.5
66	37220.5
67	28568.0
68	21666.5
69	15385.0
70	10644.5
71	7307.5
72	4882.5
73	3245.0
74	2046.5
75	1301.0
76	876.5
77	577.5
78	406.5
79	279.0
80	186.5
81	141.5
82	103.5
83	69.0
84	49.0
85	37.5
86	28.0
87	20.0
88	14.0
89	9.0
90	6.5
91	5.0
92	3.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.13786419477049E-4
10-11	0.0015550810661863364
12-13	1.6119742759248607E-4
14-15	0.0013227671264206945
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	175.0
20-21	662.0
22-23	1369.0
24-25	3293.0
26-27	4482.0
28-29	8168.0
30-31	20338.0
32-33	26107.0
34-35	36398.0
36-37	53028.0
38-39	38866.0
40-41	66466.0
42-43	76942.0
44-45	92170.0
46-47	90760.0
48-49	82179.0
50-51	44409.0
52-53	12744.0
54-55	11684.0
56-57	11219.0
58-59	47011.0
60-61	85489.0
62-63	82788.0
64-65	86721.0
66-67	91983.0
68-69	93969.0
70-71	93151.0
72-73	89247.0
74-75	91583.0
76-77	89224.0
78-79	96228.0
80-81	98172.0
82-83	94498.0
84-85	94734.0
86-87	99726.0
88-89	107164.0
90-91	111616.0
92-93	115720.0
94-95	137431.0
96-97	376312.0
98-99	700622.0
100-101	6981226.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.7945953603448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.30644737397397	70.53889087929464
2	8.436640978496282	13.63269988116221
3	2.2629133428913684	5.484935036232998
4	0.8580423042826288	2.7730072310629135
5	0.4084619836604871	1.650076034496641
6	0.2348523197905943	1.1384878888151617
7	0.14405395054449252	0.8147146451010978
8	0.0856170960840212	0.5533918907229003
9	0.06026925599678737	0.43824871358365225
>10	0.1963214344647883	2.4816254056529883
>50	0.004797500001782837	0.254544143873738
>100	0.0014692469269511185	0.1686905096638227
>500	9.057053481904718E-5	0.047893670596713345
>1k	2.264235108528607E-5	0.022794069740524198
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.482201623087416E-6
2	0.0	1.896440324617483E-5	0.0	0.0	9.482201623087416E-6
3	0.0	1.896440324617483E-5	0.0	0.0	9.482201623087416E-6
4	0.0	7.585761298469932E-5	0.0	0.0	9.482201623087416E-6
5	0.0	7.585761298469932E-5	0.0	0.0	1.896440324617483E-5
6	0.0	7.585761298469932E-5	0.0	9.482201623087416E-6	2.844660486926225E-5
7	0.0	7.585761298469932E-5	0.0	9.482201623087416E-6	3.792880649234966E-5
8	0.0	9.482201623087416E-5	0.0	9.482201623087416E-6	4.741100811543708E-5
9	0.0	1.4223302434631125E-4	0.0	9.482201623087416E-6	4.741100811543708E-5
10-11	0.0	1.4223302434631125E-4	0.0	9.482201623087416E-6	4.741100811543708E-5
12-13	0.0	1.4223302434631125E-4	0.0	9.482201623087416E-6	5.6893209738524494E-5
14-15	0.0	2.1809063733101058E-4	0.0	1.4223302434631123E-5	9.482201623087417E-5
16-17	0.0	2.27572838954098E-4	0.0	2.3705504057718542E-5	1.232686211001364E-4
18-19	0.0	2.370550405771854E-4	0.0	2.844660486926225E-5	1.564563267809424E-4
20-21	0.0	2.465372422002728E-4	0.0	3.318770568080596E-5	1.8016183083866092E-4
22-23	0.0	2.7024274625799135E-4	0.0	7.111651217315563E-5	2.228317381425543E-4
24-25	0.0	3.0817155275034104E-4	0.0	2.1809063733101055E-4	2.939482503157099E-4
26-27	0.0	3.129126535618847E-4	0.0	4.361812746620211E-4	3.034304519387973E-4
28-29	0.0	3.129126535618847E-4	0.0	0.0017352428970249972	3.0817155275034104E-4
30-31	0.0	3.461003592426907E-4	0.0	0.0063578161882801125	3.176537543734284E-4
32-33	0.0	3.508414600542344E-4	0.0	0.01683564898179171	3.2239485518497214E-4
34-35	0.0	3.8877026654658405E-4	0.0	0.02855090908711621	3.508414600542344E-4
36-37	0.0	4.2669907303893374E-4	0.0	0.048866526064581	3.508414600542344E-4
38-39	0.0	4.3618127466202116E-4	0.0	0.19209992268212794	3.6980586330040926E-4
40-41	0.0	4.835922827774582E-4	0.0	0.3434880126955301	3.935113673581278E-4
42-43	0.0	4.930744844005456E-4	0.0	0.3848351528730028	4.0299356898121515E-4
44-45	0.0	5.167799884582642E-4	0.0	0.4397939934804175	4.077346697927589E-4
46-47	0.0	5.310032908928953E-4	0.0	0.49499463022922086	4.172168714158463E-4
48-49	0.0	5.310032908928953E-4	0.0	0.5487776778353727	4.3618127466202116E-4
50-51	0.0	5.973787022545071E-4	0.0	0.5984359677354815	4.3618127466202116E-4
52-53	0.0	6.116020046891383E-4	0.0	1.059403717440253	4.6936898034282705E-4
54-55	0.0	6.163431055006821E-4	0.0	1.6778234250963913	5.072977868351767E-4
56-57	0.0	6.258253071237694E-4	0.0	2.3606462461765396	5.262621900813516E-4
58-59	0.0	6.447897103699443E-4	0.0	3.067928406343441	5.452265933275264E-4
60-61	0.0	6.542719119930318E-4	0.0	3.263323394089592	5.736731981967886E-4
62-63	0.0	6.637541136161191E-4	0.0	3.270823815573454	6.163431055006821E-4
64-65	0.0	7.396117266008185E-4	0.0	3.2775656609274693	6.258253071237694E-4
66-67	0.0	7.490939282239059E-4	0.0	3.2824679591666053	6.305664079353132E-4
68-69	0.0	7.680583314700806E-4	0.0	3.287740063269042	6.542719119930318E-4
70-71	0.0	7.775405330931681E-4	0.0	3.292670808113048	6.82718516862294E-4
72-73	0.0	7.870227347162556E-4	0.0	3.2965063586695864	7.253884241661874E-4
74-75	0.0	8.10728238773974E-4	0.0	3.299469546676801	7.30129524977731E-4
76-77	0.0	8.581392468894112E-4	0.0	3.3023142071637275	7.348706257892748E-4
78-79	0.0	8.723625493240423E-4	0.0	3.304352880512691	7.396117266008185E-4
80-81	0.0	8.771036501355859E-4	0.0	3.3050166346263072	7.585761298469934E-4
82-83	0.0	8.865858517586734E-4	0.0	3.305599790026127	7.680583314700807E-4
84-85	0.0	9.150324566279356E-4	0.0	3.305832103965893	7.680583314700807E-4
86-87	0.0	9.482201623087416E-4	0.0	3.305936408183747	7.680583314700807E-4
88	0.0	0.001005113372047266	0.0	3.3059506314861813	7.680583314700807E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13695	0.0	53.425705	1
ATCTCGT	8775	0.0	43.119488	94
GTATCAA	25295	0.0	38.646633	1
TATCTCG	11060	0.0	33.26886	94
TCAACGC	31220	0.0	30.690369	4
ATCAACG	31640	0.0	30.337515	3
CAACGCA	31940	0.0	30.026548	5
TATCAAC	32405	0.0	29.807701	2
TATGCCG	4425	0.0	29.124475	86-87
AACGCAG	33115	0.0	29.091415	6
GCCGTCT	4990	0.0	27.187576	90-91
CCGTCTT	4960	0.0	26.606575	90-91
ATGCCGT	5005	0.0	26.520643	88-89
TCGTATG	5020	0.0	26.467896	84-85
CTCGTAT	5000	0.0	25.80721	82-83
TGCCGTC	5200	0.0	25.311617	88-89
TCTCGTA	5265	0.0	25.220133	82-83
ACGCAGA	38400	0.0	24.975325	7
CGCTATC	17640	0.0	24.88318	94
CGTATGC	5315	0.0	24.437641	84-85
>>END_MODULE