FastQCFastQC Report
Fri 27 May 2016
SRR2139551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5245755
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78150.050.6711461
GTATCAA130050.035.07511
TCAACGC161800.027.3901394
ATCAACG163050.027.2064363
CAACGCA164600.027.0038575
TATCAAC168200.026.6049942
AACGCAG167300.026.568056
GTGGTAT37200.023.5004981
ACGCAGA194550.022.8469667
TGGTATC36700.022.5235022
CGCAGAG199900.022.2790188
GTACATG167000.022.079631
TACATGG168000.021.278422
ACATGGG166800.020.653123
GCAGAGT225550.019.7837839
GAGTACT140500.019.68346212-13
CAGAGTA221200.018.85202410-11
AGAGTAC207450.018.65157310-11
CATGGGG115750.018.1339954
GTACTTT155050.017.73890914-15
Produced by FastQC (version 0.11.5)