##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139551_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5245755
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.630389333851845	33.0	33.0	33.0	33.0	33.0
2	32.63147020781565	33.0	33.0	33.0	33.0	33.0
3	32.623726041341996	33.0	33.0	33.0	33.0	33.0
4	36.53347173095198	37.0	37.0	37.0	37.0	37.0
5	36.55195448510272	37.0	37.0	37.0	37.0	37.0
6	36.53626008077007	37.0	37.0	37.0	37.0	37.0
7	36.5062291319362	37.0	37.0	37.0	37.0	37.0
8	36.504242382650354	37.0	37.0	37.0	37.0	37.0
9	36.542080596596676	37.0	37.0	37.0	37.0	37.0
10-11	36.475700066053406	37.0	37.0	37.0	37.0	37.0
12-13	36.473594840018265	37.0	37.0	37.0	37.0	37.0
14-15	38.50100776723274	40.0	37.0	40.0	37.0	40.0
16-17	38.43439161760318	40.0	37.0	40.0	37.0	40.0
18-19	38.44755149640042	40.0	37.0	40.0	37.0	40.0
20-21	38.4485656918578	40.0	37.0	40.0	37.0	40.0
22-23	38.45192320153311	40.0	37.0	40.0	37.0	40.0
24-25	38.42968077267493	40.0	37.0	40.0	37.0	40.0
26-27	38.31185182041389	40.0	37.0	40.0	37.0	40.0
28-29	38.25774252350374	40.0	37.0	40.0	37.0	40.0
30-31	38.19168500151507	40.0	37.0	40.0	37.0	40.0
32-33	38.11708238068488	40.0	37.0	40.0	35.0	40.0
34-35	38.0690604479014	40.0	37.0	40.0	33.0	40.0
36-37	38.02454491922738	40.0	37.0	40.0	33.0	40.0
38-39	37.958388972292965	40.0	37.0	40.0	33.0	40.0
40-41	37.902852777946066	40.0	37.0	40.0	33.0	40.0
42-43	37.847172962122116	40.0	37.0	40.0	33.0	40.0
44-45	37.80320419391363	40.0	37.0	40.0	33.0	40.0
46-47	37.739113291050046	40.0	37.0	40.0	33.0	40.0
48-49	37.72804773101706	40.0	37.0	40.0	33.0	40.0
50-51	37.64533877333075	40.0	37.0	40.0	33.0	40.0
52-53	37.52678430971936	40.0	37.0	40.0	33.0	40.0
54-55	37.36823322065484	40.0	37.0	40.0	33.0	40.0
56-57	37.27800134396699	40.0	37.0	40.0	33.0	40.0
58-59	37.14648962136276	37.0	37.0	40.0	33.0	40.0
60-61	37.00142895283031	37.0	37.0	40.0	33.0	40.0
62-63	36.812293943029275	37.0	37.0	40.0	33.0	40.0
64-65	36.63883217876068	37.0	37.0	40.0	33.0	40.0
66-67	36.43927405544377	37.0	37.0	40.0	33.0	40.0
68-69	36.162618572883865	37.0	37.0	40.0	33.0	40.0
70-71	35.928473320932	37.0	37.0	38.5	33.0	40.0
72-73	35.60348776659832	37.0	33.0	37.0	33.0	40.0
74-75	35.511312736854705	37.0	33.0	37.0	33.0	40.0
76-77	33.85264794054833	35.0	33.0	37.0	27.0	37.0
78-79	35.07844513853615	37.0	33.0	37.0	33.0	37.0
80-81	35.12300497221773	37.0	33.0	37.0	33.0	37.0
82-83	34.95966542788426	37.0	33.0	37.0	33.0	37.0
84-85	34.8240034755885	37.0	33.0	37.0	33.0	37.0
86-87	34.71538760652949	37.0	33.0	37.0	33.0	37.0
88-89	34.597692450451916	37.0	33.0	37.0	33.0	37.0
90-91	34.49713395449995	37.0	33.0	37.0	33.0	37.0
92-93	34.45716922527709	37.0	33.0	37.0	33.0	37.0
94-95	34.468416423188444	37.0	33.0	37.0	33.0	37.0
96-97	34.45570925428956	37.0	33.0	37.0	33.0	37.0
98-99	34.46200027102171	37.0	33.0	37.0	33.0	37.0
100	34.454180508219245	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	10.0
20	31.0
21	193.0
22	710.0
23	2146.0
24	5090.0
25	10337.0
26	18101.0
27	28352.0
28	41717.0
29	58640.0
30	79538.0
31	106278.0
32	140125.0
33	186797.0
34	255652.0
35	373434.0
36	651780.0
37	1520101.0
38	1722470.0
39	44251.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.90041280997683	20.056540955496395	14.51512699315923	23.52791924136754
2	14.540328322615142	20.506390405194296	42.436884681042095	22.516396591148464
3	18.37992815142911	25.68577831027183	30.474450293618364	25.459843244680698
4	12.232061924355978	18.00133631860428	38.83267518212345	30.93392657491629
5	13.185042000627172	37.79829595549163	35.63429859000277	13.38236345387842
6	30.037296823812774	37.92628515818981	18.560073812063276	13.476344205934133
7	26.635250788494698	32.606841150606535	22.645510512786053	18.112397548112714
8	24.332112346077924	37.55573792523669	20.550807271784517	17.561342456900867
9	25.157584711433067	17.01215269503884	21.155754658533098	36.67450793499499
10-11	24.36175931768487	26.891588681712726	28.516290578705867	20.230361421896536
12-13	25.072392019130174	24.330962395419984	28.85396237406934	21.742683211380502
14-15	22.08330894818383	25.788796836405737	26.595889683680607	25.53200453172983
16-17	21.582489079265045	29.045647385362066	27.254684978616044	22.117178556756844
18-19	21.5920968478322	27.972951462658855	29.142535249930656	21.29241643957829
20-21	22.40500349434785	27.01172827432594	29.254163573606444	21.329104657719764
22-23	22.136369750209404	27.076635310538343	29.13157210147577	21.65542283777648
24-25	21.936094060755835	27.259218156197146	29.156460289039277	21.648227494007745
26-27	21.86628111855366	27.348105789849892	29.114296354825065	21.67131673677138
28-29	21.719982375771227	27.300170803888857	29.20683528501885	21.773011535321064
30-31	22.042842752140285	27.23516565123069	29.12250516567797	21.599486430951053
32-33	21.826055060476822	27.394719737048806	29.116806669145195	21.662418533329173
34-35	21.920122691962455	27.448558159444126	29.13983970610389	21.491479442489535
36-37	21.960857623615542	27.316286014495134	28.923327281909412	21.799529079979916
38-39	21.765000468318338	27.41498813931857	29.075222557536218	21.744788834826878
40-41	22.00755474723509	27.313923147277535	28.931521790252756	21.747000315234615
42-43	21.863306683947474	27.42434394275049	28.812291656587014	21.900057716715025
44-45	21.928172138350714	27.538600491994845	28.635460402604235	21.897766967050206
46-47	22.10992130047736	27.53848138701706	28.221807607951334	22.129789704554252
48-49	22.038709251544127	27.646754950924574	28.148856040250504	22.165679757280802
50-51	22.009865960923946	27.85369772085727	28.008783172971597	22.12765314524719
52-53	22.149347608966828	27.81218466010444	28.002826134468762	22.035641596459975
54-55	22.111536041505346	27.713816275570135	27.985077136654446	22.18957054627007
56-57	22.157547627446846	27.830096794083847	27.85441815278101	22.1579374256883
58-59	22.04912670498218	27.887715287212462	27.962131222819593	22.101026784985766
60-61	22.17586735341865	27.961773945399287	27.841789635415164	22.0205690657669
62-63	22.074374472022203	28.06764526931896	27.812644565348567	22.04533569331027
64-65	22.0498917678467	27.979064039019068	27.790991088321594	22.180053104812636
66-67	22.159588323790995	27.953580105151797	27.64587552961315	22.240956041444058
68-69	22.1699318413802	27.948131457524433	27.697739949508005	22.184196751587358
70-71	22.116517371153016	27.743664937936284	27.793056205685463	22.346761485225237
72-73	22.201265620051153	27.726107286891118	27.67514147884026	22.39748561421747
74-75	22.116642533731177	27.875257092269567	27.69499772015879	22.31310265384047
76-77	22.254383362958766	27.827585734348954	27.614401982553634	22.30362892013865
78-79	22.1865383308849	27.75593554846952	27.586809867539564	22.470716253106023
80-81	22.177851535868193	27.829770541216902	27.63578671350078	22.356591209414127
82-83	22.250648168621193	27.761608584691217	27.72614729840871	22.26159594827888
84-85	22.326638141174897	27.770439516150237	27.65641070307176	22.24651163960311
86-87	22.21525550715751	27.776742635120133	27.72580285497982	22.28219900274254
88-89	22.273742547183804	27.721984896805544	27.717432936587123	22.286839619423528
90-91	22.325406563434207	27.744487065030143	27.676229269931103	22.25387710160455
92-93	22.3081418081149	27.655913727570724	27.794167162356587	22.24177730195779
94-95	22.176617621824995	27.69085531295403	27.86976474079476	22.26276232442621
96-97	22.29416463785291	27.813892169505046	27.901127990585206	21.99081520205684
98-99	23.34414238680209	29.683063253082853	28.184776247015357	18.7880181130997
100	22.28347852068311	30.77260553543405	22.03955375789633	24.90436218598651
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	957.0
1	838.0
2	1222.0
3	1857.0
4	2560.5
5	4288.0
6	5873.0
7	7383.0
8	8602.0
9	8751.0
10	7825.5
11	6614.0
12	6924.5
13	9144.5
14	14729.5
15	22460.5
16	28706.0
17	32405.0
18	33547.5
19	32318.5
20	29435.0
21	26199.0
22	24238.0
23	24936.5
24	28639.5
25	34955.5
26	43234.0
27	52473.5
28	62371.0
29	73564.5
30	84848.5
31	95513.5
32	106831.0
33	118132.5
34	129513.5
35	140463.5
36	151603.5
37	161889.5
38	170654.0
39	178925.0
40	185772.0
41	191142.5
42	195680.0
43	199868.0
44	202989.0
45	206661.0
46	209509.5
47	210493.0
48	212827.5
49	212225.5
50	208020.5
51	203124.5
52	195790.5
53	185758.0
54	173597.5
55	160262.5
56	147251.5
57	133671.0
58	118290.0
59	102320.0
60	85946.0
61	70179.5
62	55644.5
63	42995.5
64	31998.0
65	23294.5
66	17342.0
67	13102.5
68	9906.5
69	7238.5
70	5228.5
71	3773.0
72	2636.5
73	1726.5
74	1138.0
75	766.0
76	507.0
77	341.5
78	232.0
79	152.5
80	103.5
81	67.0
82	50.0
83	36.0
84	23.5
85	18.5
86	18.5
87	15.5
88	9.5
89	6.5
90	3.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	9.531516435670367E-5
10-11	0.0016203577940639622
12-13	2.0969336158474804E-4
14-15	0.0014487904982218957
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	87.0
20-21	353.0
22-23	669.0
24-25	1428.0
26-27	1911.0
28-29	3737.0
30-31	8827.0
32-33	10951.0
34-35	15700.0
36-37	23015.0
38-39	26667.0
40-41	28944.0
42-43	33885.0
44-45	41209.0
46-47	39918.0
48-49	36279.0
50-51	30622.0
52-53	28037.0
54-55	30532.0
56-57	33796.0
58-59	33453.0
60-61	34606.0
62-63	33554.0
64-65	35117.0
66-67	38014.0
68-69	36680.0
70-71	36891.0
72-73	34000.0
74-75	34431.0
76-77	33898.0
78-79	37750.0
80-81	39317.0
82-83	37871.0
84-85	37343.0
86-87	40427.0
88-89	44969.0
90-91	48667.0
92-93	52141.0
94-95	59720.0
96-97	174586.0
98-99	331202.0
100-101	3594551.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.78390461302324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.11178432851193	79.98148184616436
2	6.618747798070768	11.620390507270036
3	1.34939214349096	3.5536473362932015
4	0.44054977975811255	1.5469271937429818
5	0.2147586281868968	0.9426175465791137
6	0.09123395186154158	0.4805323516609632
7	0.0543766138396426	0.334137403773487
8	0.03158672572260063	0.22182448942964006
9	0.020891192802994486	0.1650519428643311
>10	0.06464634877051588	0.9001400661401837
>50	0.0011999282757754054	0.07152325608524872
>100	8.009276590532083E-4	0.16606449868557777
>500	3.1633049191547164E-5	0.015661561310873144
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.906303287134073E-5	0.0
3	0.0	0.0	0.0	1.906303287134073E-5	0.0
4	1.906303287134073E-5	1.906303287134073E-5	0.0	5.718909861402219E-5	0.0
5	1.906303287134073E-5	1.906303287134073E-5	0.0	5.718909861402219E-5	0.0
6	7.625213148536292E-5	1.906303287134073E-5	0.0	5.718909861402219E-5	3.812606574268146E-5
7	7.625213148536292E-5	1.906303287134073E-5	0.0	5.718909861402219E-5	3.812606574268146E-5
8	7.625213148536292E-5	1.906303287134073E-5	0.0	5.718909861402219E-5	3.812606574268146E-5
9	7.625213148536292E-5	1.1437819722804438E-4	0.0	9.531516435670365E-5	7.625213148536292E-5
10-11	7.625213148536292E-5	1.1437819722804438E-4	0.0	1.1437819722804438E-4	7.625213148536292E-5
12-13	7.625213148536292E-5	1.2390971366371475E-4	0.0	1.5250426297072585E-4	7.625213148536292E-5
14-15	7.625213148536292E-5	1.7156729584206658E-4	0.0	1.906303287134073E-4	7.625213148536292E-5
16-17	1.7156729584206658E-4	1.906303287134073E-4	0.0	2.0016184514907767E-4	7.625213148536292E-5
18-19	1.7156729584206658E-4	1.906303287134073E-4	0.0	2.6688246019877023E-4	7.625213148536292E-5
20-21	1.7156729584206658E-4	1.906303287134073E-4	0.0	4.3844975604083686E-4	7.625213148536292E-5
22-23	1.7156729584206658E-4	2.0969336158474804E-4	0.0	8.578364792103329E-4	7.625213148536292E-5
24-25	1.8109881227773694E-4	2.192248780204184E-4	0.0	0.0016584838598066435	7.625213148536292E-5
26-27	1.906303287134073E-4	2.2875639445608877E-4	0.0	0.0036410392784260795	7.625213148536292E-5
28-29	1.906303287134073E-4	2.2875639445608877E-4	0.0	0.010589514760029776	7.625213148536292E-5
30-31	2.0969336158474804E-4	2.6688246019877023E-4	0.0	0.01406851825904946	8.578364792103329E-5
32-33	2.0969336158474804E-4	2.6688246019877023E-4	0.0	0.021541227144615024	1.1437819722804438E-4
34-35	2.2875639445608877E-4	3.145400423771221E-4	0.0	0.030596167758501873	1.5250426297072585E-4
36-37	2.478194273274295E-4	3.90792173862485E-4	0.0	0.04416904716289648	1.5250426297072585E-4
38-39	2.478194273274295E-4	4.4798127247650714E-4	0.0	0.07396456754080204	1.5250426297072585E-4
40-41	2.8594549307011096E-4	4.5751278891217754E-4	0.0	0.10458932984861091	1.620357794063962E-4
42-43	2.8594549307011096E-4	4.6704430534784793E-4	0.0	0.1247008295278754	1.906303287134073E-4
44-45	2.954770095057813E-4	4.95638854654859E-4	0.0	0.15029295115765035	2.0969336158474804E-4
46-47	3.050085259414517E-4	5.147018875261998E-4	0.0	0.17417893134544027	2.192248780204184E-4
48-49	3.050085259414517E-4	5.528279532688812E-4	0.0	0.19740723689916895	2.2875639445608877E-4
50-51	3.050085259414517E-4	5.909540190115627E-4	0.0	0.21814781666318767	2.2875639445608877E-4
52-53	3.240715588127924E-4	5.909540190115627E-4	0.0	0.25945740889538305	2.3828791089175913E-4
54-55	3.240715588127924E-4	5.909540190115627E-4	0.0	0.30608558729868246	2.478194273274295E-4
56-57	3.240715588127924E-4	5.909540190115627E-4	0.0	0.3496255543768247	2.5735094376309984E-4
58-59	3.240715588127924E-4	6.195485683185738E-4	0.0	0.3781438515523504	2.6688246019877023E-4
60-61	3.240715588127924E-4	6.290800847542442E-4	0.0	0.40074307702132483	2.6688246019877023E-4
62-63	3.4313459168413315E-4	6.290800847542442E-4	0.0	0.4176901132439468	2.6688246019877023E-4
64-65	3.5266610811980354E-4	6.862691833682663E-4	0.0	0.42393325650931085	2.6688246019877023E-4
66-67	3.621976245554739E-4	6.862691833682663E-4	0.0	0.4295187251406137	2.764139766344406E-4
68-69	3.621976245554739E-4	6.862691833682663E-4	0.0	0.4347324646309254	2.8594549307011096E-4
70-71	3.621976245554739E-4	6.862691833682663E-4	0.0	0.43885961124757067	3.050085259414517E-4
72-73	3.812606574268146E-4	6.862691833682663E-4	0.0	0.44235767777946167	3.050085259414517E-4
74-75	3.812606574268146E-4	7.243952491109478E-4	0.0	0.4448835296349143	3.050085259414517E-4
76-77	4.0032369029815534E-4	7.911158641606404E-4	0.0	0.44686608505353376	3.050085259414517E-4
78-79	4.0032369029815534E-4	8.197104134676515E-4	0.0	0.448391127683241	3.050085259414517E-4
80-81	4.0032369029815534E-4	8.387734463389921E-4	0.0	0.4488772350214602	3.145400423771221E-4
82-83	4.0032369029815534E-4	8.387734463389921E-4	0.0	0.44922990112958	3.240715588127924E-4
84-85	4.0032369029815534E-4	8.387734463389921E-4	0.0	0.4493824053925507	3.240715588127924E-4
86-87	4.0032369029815534E-4	8.673679956460033E-4	0.0	0.4494872520733431	3.240715588127924E-4
88	4.0032369029815534E-4	8.768995120816736E-4	0.0	0.44950631510621447	3.240715588127924E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7815	0.0	50.671146	1
GTATCAA	13005	0.0	35.0751	1
TCAACGC	16180	0.0	27.390139	4
ATCAACG	16305	0.0	27.206436	3
CAACGCA	16460	0.0	27.003857	5
TATCAAC	16820	0.0	26.604994	2
AACGCAG	16730	0.0	26.56805	6
GTGGTAT	3720	0.0	23.500498	1
ACGCAGA	19455	0.0	22.846966	7
TGGTATC	3670	0.0	22.523502	2
CGCAGAG	19990	0.0	22.279018	8
GTACATG	16700	0.0	22.07963	1
TACATGG	16800	0.0	21.27842	2
ACATGGG	16680	0.0	20.65312	3
GCAGAGT	22555	0.0	19.783783	9
GAGTACT	14050	0.0	19.683462	12-13
CAGAGTA	22120	0.0	18.852024	10-11
AGAGTAC	20745	0.0	18.651573	10-11
CATGGGG	11575	0.0	18.133995	4
GTACTTT	15505	0.0	17.738909	14-15
>>END_MODULE