FastQCFastQC Report
Fri 27 May 2016
SRR2139550_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139550_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9058056
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155150.055.045291
CTTATAC142100.043.244851
GTATCAA277100.035.755111
TTATACA184650.034.291642
TATACAC202600.033.4755553
ATCAACG344150.028.20923
TCAACGC345500.028.123564
CAACGCA351850.027.6881145
TATCAAC353200.027.6064852
AACGCAG361950.026.9958386
TGGTATC62300.026.2441562
GTGGTAT67000.025.8609981
TACACAT273300.024.8310265
ACACATC257450.024.4116746
ACGCAGA409100.023.843367
CGCAGAG416600.023.3938568
CACATCT279050.022.4615787
GTACATG334600.021.2212161
CATCTCC295700.021.1683469
ATACACA340200.021.096714
Produced by FastQC (version 0.11.5)