##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139550_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9058056
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.495669269432646	33.0	33.0	33.0	33.0	33.0
2	32.50754532760672	33.0	33.0	33.0	33.0	33.0
3	32.49914065446272	33.0	33.0	33.0	33.0	33.0
4	36.387171265004326	37.0	37.0	37.0	37.0	37.0
5	36.412311758726155	37.0	37.0	37.0	37.0	37.0
6	36.399706184196695	37.0	37.0	37.0	37.0	37.0
7	36.372822601229224	37.0	37.0	37.0	37.0	37.0
8	36.358633574356354	37.0	37.0	37.0	37.0	37.0
9	36.40776884134962	37.0	37.0	37.0	37.0	37.0
10-11	36.34683104189243	37.0	37.0	37.0	37.0	37.0
12-13	36.32508305314076	37.0	37.0	37.0	35.0	37.0
14-15	38.18376779741702	40.0	37.0	40.0	35.0	40.0
16-17	38.119746665288886	40.0	37.0	40.0	33.0	40.0
18-19	38.1503582556787	40.0	37.0	40.0	33.0	40.0
20-21	38.16383970531412	40.0	37.0	40.0	33.0	40.0
22-23	38.16711123625241	40.0	37.0	40.0	33.0	40.0
24-25	38.13960404733531	40.0	37.0	40.0	33.0	40.0
26-27	37.99960612065816	40.0	37.0	40.0	33.0	40.0
28-29	37.91917258917971	40.0	37.0	40.0	33.0	40.0
30-31	37.865846673925404	40.0	37.0	40.0	33.0	40.0
32-33	37.781861647604735	40.0	37.0	40.0	33.0	40.0
34-35	37.70996989425777	40.0	37.0	40.0	33.0	40.0
36-37	37.6557541814921	40.0	37.0	40.0	33.0	40.0
38-39	37.58565201576995	40.0	37.0	40.0	33.0	40.0
40-41	37.57022254155261	40.0	37.0	40.0	33.0	40.0
42-43	37.51288418268628	40.0	37.0	40.0	33.0	40.0
44-45	37.418076165499656	40.0	37.0	40.0	33.0	40.0
46-47	37.37021301360348	40.0	37.0	40.0	33.0	40.0
48-49	37.386306946871336	40.0	37.0	40.0	33.0	40.0
50-51	37.26403930395027	40.0	37.0	40.0	33.0	40.0
52-53	37.16744023077071	40.0	37.0	40.0	33.0	40.0
54-55	37.046864959715116	38.5	37.0	40.0	33.0	40.0
56-57	36.92530920841493	37.0	37.0	40.0	33.0	40.0
58-59	36.808999209878856	37.0	37.0	40.0	33.0	40.0
60-61	36.64664971747449	37.0	37.0	40.0	33.0	40.0
62-63	36.480524294111234	37.0	37.0	40.0	33.0	40.0
64-65	36.35096045769288	37.0	37.0	40.0	33.0	40.0
66-67	36.1406599020762	37.0	37.0	40.0	33.0	40.0
68-69	35.95414059015835	37.0	37.0	40.0	33.0	40.0
70-71	35.70872919478808	37.0	33.0	37.0	33.0	40.0
72-73	35.500463902118256	37.0	33.0	37.0	33.0	40.0
74-75	35.30285097314644	37.0	33.0	37.0	33.0	40.0
76-77	33.533615305148196	35.0	33.0	37.0	27.0	37.0
78-79	34.7903052546871	37.0	33.0	37.0	30.0	37.0
80-81	34.880276509957035	37.0	33.0	37.0	33.0	37.0
82-83	34.733873198416994	37.0	33.0	37.0	33.0	37.0
84-85	34.56160495575991	37.0	33.0	37.0	27.0	37.0
86-87	34.42506161724138	37.0	33.0	37.0	27.0	37.0
88-89	34.29463821581498	37.0	33.0	37.0	27.0	37.0
90-91	34.22684749416596	37.0	33.0	37.0	27.0	37.0
92-93	34.184192064401564	37.0	33.0	37.0	27.0	37.0
94-95	34.193090581453234	37.0	33.0	37.0	27.0	37.0
96-97	34.157802827119255	37.0	33.0	37.0	27.0	37.0
98-99	34.19298661925187	37.0	33.0	37.0	27.0	37.0
100	34.19853210312787	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	4.0
19	19.0
20	99.0
21	515.0
22	1862.0
23	5396.0
24	12288.0
25	23636.0
26	40278.0
27	63029.0
28	92191.0
29	127350.0
30	169507.0
31	222932.0
32	292012.0
33	384190.0
34	516433.0
35	725518.0
36	1191491.0
37	2513797.0
38	2611032.0
39	64474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.65524655621471	20.718198253576706	15.032596398167552	23.59395879204103
2	14.232192867873636	20.742949701348724	42.78633296150962	22.23852446926802
3	17.75192160437074	26.53683086083813	31.359311534395463	24.351936000395668
4	11.821437427121902	18.85225703786316	38.38348789028492	30.942817644730024
5	12.744302677188582	38.05229352490535	35.815219623442104	13.388184174463962
6	28.930405037284494	38.38600008500721	18.71826788422018	13.965326993488118
7	25.68982792775845	33.31230233065462	22.509465607189888	18.488404134397047
8	24.148205751874354	37.37793186529207	20.936335566925177	17.537526815908404
9	24.744772520373708	16.91529408595586	20.954210853215027	37.385722540455404
10-11	23.688492013111105	27.05104454971699	28.637801847433806	20.622661589738097
12-13	24.771308604188434	24.634721392226872	28.738233125401784	21.855736878182906
14-15	22.008571022929882	26.21006248425418	26.34683718037236	25.434529312443573
16-17	21.351811028768203	29.639836627196832	27.048811577230257	21.959540766804707
18-19	21.191522772656736	27.869771394656865	29.239182226296677	21.699523606389715
20-21	21.838349962631483	26.8060359524931	29.612618839418165	21.74299524545725
22-23	21.54007058663756	27.284457082982083	29.485086323162697	21.690386007217658
24-25	21.35992697192801	27.67489830094439	29.699652617494493	21.265522109633107
26-27	21.14485196641626	27.663593680954484	29.71460450729121	21.476949845338048
28-29	20.668571140245295	26.94854798857272	30.03993072183071	22.342950149351278
30-31	20.937623447944663	26.9510888869463	30.19108773614866	21.920199928960376
32-33	20.934156841274035	27.36011774283073	30.10594437640274	21.599781039492502
34-35	21.06728405701993	27.85870208504874	30.196350432356496	20.877663425574834
36-37	21.303259809557588	27.467174158961367	29.312361592947127	21.91720443853392
38-39	21.380640834427318	27.207232804173753	29.693203575953625	21.718922785445308
40-41	21.319419465454377	27.120511284887154	29.92598220648439	21.634087043174073
42-43	21.56021664810991	27.11789064118848	29.911216614662887	21.41067609603872
44-45	21.324717924216777	27.5387708011404	29.43502618789447	21.70148508674835
46-47	21.991318697543335	27.221321878197923	28.540964021605326	22.246395402653413
48-49	21.66345352400313	27.37610383234349	28.803274698729957	22.15716794492342
50-51	21.54458032327435	27.814524387396762	28.698817477913263	21.94207781141563
52-53	21.537836141960888	28.014484044423675	28.92928637574726	21.51839343786818
54-55	21.51826104802225	28.087228873747883	28.438571737356376	21.955938340873494
56-57	21.929961673291647	28.227763019418077	28.001695985187446	21.84057932210283
58-59	21.446123785009217	28.48780166916192	28.56564391618376	21.500430629645106
60-61	21.844492541621253	28.890828092662485	27.828192973203496	21.43648639251276
62-63	21.503307437573653	29.060638718949782	27.904633995069634	21.531419848406934
64-65	21.590978553846814	28.692741814778994	27.996424173742646	21.719855457631553
66-67	21.76355962932413	28.405418216906313	27.95952223065147	21.871499923118087
68-69	21.757839696258117	28.23812296255014	28.079350901054127	21.92468644013762
70-71	21.77852332651669	28.07141857673851	28.13885560288643	22.011202493858367
72-73	21.79774687621891	28.04534181339215	28.095031705229996	22.061879605158943
74-75	21.737130075528082	28.185707136361938	28.02659109761588	22.0505716904941
76-77	21.868015435836803	28.076641350913036	28.019698237496648	22.035644975753513
78-79	21.83613878715817	28.008009393756673	27.997515680701607	22.158336138383554
80-81	21.79773035179262	28.11709771666939	27.98426836312143	22.10090356841656
82-83	21.911989307913892	28.079916137809874	28.0224753414622	21.985619212814033
84-85	21.94200657617939	28.059206408551834	27.9578701984346	22.040916816834176
86-87	21.85259478781104	28.044867833124627	28.085248294667842	22.017289084396495
88-89	21.916058359263175	27.941947567267366	28.073405854016	22.06858821945346
90-91	21.946398287972553	27.927892449969864	28.087286773061535	22.038422488996044
92-93	21.882388437322785	27.87952394108153	28.160295464789964	22.077792156805724
94-95	21.765839900666784	27.912986487031873	28.232127424997085	22.089046187304263
96-97	21.86135564672174	28.05689582895879	28.22609766322046	21.855650861099008
98-99	22.947210518404347	29.892007149009775	28.55013641532768	18.610645917258196
100	21.94068337162553	30.93205177147912	22.360800510007913	24.766464346887442
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3055.0
1	2515.5
2	2996.0
3	4225.0
4	5569.5
5	9156.5
6	12469.5
7	15571.0
8	18216.0
9	18636.5
10	16824.0
11	14411.5
12	14972.0
13	19623.0
14	31424.0
15	48190.0
16	61770.5
17	69051.5
18	70628.5
19	67346.5
20	60947.0
21	53607.5
22	48160.5
23	47980.5
24	53611.5
25	64634.0
26	79539.5
27	95924.0
28	113946.5
29	134180.5
30	153925.5
31	173464.0
32	194529.0
33	216305.5
34	239238.5
35	259561.0
36	279204.5
37	297056.0
38	312417.5
39	325535.0
40	333890.5
41	339641.5
42	342395.5
43	346003.5
44	350471.5
45	356940.5
46	371598.5
47	391245.0
48	394871.0
49	391429.5
50	392628.0
51	378090.5
52	349091.0
53	315836.0
54	286121.5
55	261648.0
56	236822.0
57	209381.0
58	181597.5
59	155619.0
60	129681.0
61	104073.5
62	81284.5
63	62560.0
64	46575.0
65	33729.0
66	24682.0
67	18559.5
68	13988.0
69	10058.5
70	7163.5
71	5117.5
72	3576.0
73	2470.5
74	1750.0
75	1233.0
76	844.0
77	577.0
78	401.5
79	271.5
80	185.5
81	132.5
82	95.0
83	68.0
84	50.5
85	39.5
86	34.0
87	26.0
88	16.5
89	9.5
90	5.0
91	3.0
92	2.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.001346867363151652
5	0.007109693294013638
6	1.1039896419275835E-5
7	0.0
8	0.0
9	6.403139923179985E-4
10-11	9.494310920577218E-4
12-13	0.0011812689168625146
14-15	0.0010377502634119286
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	192.0
20-21	618.0
22-23	1378.0
24-25	2925.0
26-27	3964.0
28-29	7843.0
30-31	17153.0
32-33	22003.0
34-35	31382.0
36-37	43295.0
38-39	50917.0
40-41	55411.0
42-43	68796.0
44-45	82687.0
46-47	79770.0
48-49	73179.0
50-51	61839.0
52-53	60450.0
54-55	79133.0
56-57	86040.0
58-59	82760.0
60-61	86281.0
62-63	87049.0
64-65	94965.0
66-67	88968.0
68-69	70865.0
70-71	69423.0
72-73	65325.0
74-75	67092.0
76-77	65401.0
78-79	72584.0
80-81	74469.0
82-83	72431.0
84-85	72782.0
86-87	78406.0
88-89	86486.0
90-91	92394.0
92-93	98734.0
94-95	112893.0
96-97	295482.0
98-99	544970.0
100-101	5849321.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.53536283141209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.64184373797711	76.67547629004619
2	7.3947313863710855	12.650220643481635
3	1.7289323468645308	4.436545668000673
4	0.5997407146247127	2.051961585207807
5	0.26434740892022845	1.13055257677677
6	0.1255533927767079	0.6443553003522302
7	0.07166441604951052	0.42908892802273074
8	0.04134101342826476	0.28288948667239244
9	0.034296749613340706	0.26402264299036704
>10	0.09530668074133358	1.2437497672637507
>50	0.0017861313511980418	0.1003410555817268
>100	3.955351882278196E-4	0.05775372945788895
>500	6.0486093819350655E-5	0.033042326145785214
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.1039896419275835E-5	0.0	0.0	0.0
2	0.0	1.1039896419275835E-5	0.0	0.0	0.0
3	0.0	2.207979283855167E-5	0.0	0.0	0.0
4	0.0	6.623937851565502E-5	0.0	0.0	0.0
5	0.0	8.831917135420668E-5	0.0	0.0	0.0
6	0.0	8.831917135420668E-5	0.0	0.0	0.0
7	0.0	8.831917135420668E-5	0.0	1.1039896419275835E-5	0.0
8	0.0	8.831917135420668E-5	0.0	1.1039896419275835E-5	0.0
9	0.0	9.935906777348253E-5	0.0	3.311968925782751E-5	0.0
10-11	1.1039896419275835E-5	9.935906777348253E-5	0.0	8.831917135420668E-5	0.0
12-13	1.1039896419275835E-5	9.935906777348253E-5	0.0	1.0487901598312045E-4	0.0
14-15	1.1039896419275835E-5	1.545585498698617E-4	0.0	1.1591891240239627E-4	2.207979283855167E-5
16-17	1.1039896419275835E-5	1.545585498698617E-4	0.0	1.6007849807949962E-4	2.207979283855167E-5
18-19	1.1039896419275835E-5	1.6007849807949962E-4	0.0	2.152779801758788E-4	2.207979283855167E-5
20-21	1.1039896419275835E-5	1.7663834270841336E-4	0.0	2.6495751406262007E-4	2.207979283855167E-5
22-23	1.1039896419275835E-5	1.9871813554696505E-4	0.0	5.354349763348781E-4	3.8639637467465425E-5
24-25	1.1039896419275835E-5	2.2631787659515464E-4	0.0	0.0012364683989588936	5.519948209637918E-5
26-27	1.1039896419275835E-5	2.3735777301443046E-4	0.0	0.0029973318778333895	5.519948209637918E-5
28-29	1.1039896419275835E-5	2.483976694337063E-4	0.0	0.009742708590010925	5.519948209637918E-5
30-31	1.1039896419275835E-5	2.870373069011717E-4	0.0	0.011774049531157679	6.0719430306017095E-5
32-33	1.1039896419275835E-5	3.2015699615899924E-4	0.0	0.015229537110391014	6.623937851565502E-5
34-35	1.1039896419275835E-5	3.6431658183610257E-4	0.0	0.019954612777841073	6.623937851565502E-5
36-37	1.1039896419275835E-5	4.0847616751320595E-4	0.0	0.027897818251510036	6.623937851565502E-5
38-39	1.1039896419275835E-5	4.4159585677103344E-4	0.0	0.046505563666199456	8.279922314456876E-5
40-41	1.1039896419275835E-5	4.747155460288609E-4	0.0	0.06536722669853222	8.831917135420668E-5
42-43	1.1039896419275835E-5	4.912753906577747E-4	0.0	0.07667208063187067	8.831917135420668E-5
44-45	1.1039896419275835E-5	5.464748727541538E-4	0.0	0.09007451488487155	8.831917135420668E-5
46-47	1.6559844628913755E-5	5.851145102216193E-4	0.0	0.10420006235333498	9.38391195638446E-5
48-49	2.207979283855167E-5	6.01674354850533E-4	0.0	0.11771841551873824	9.935906777348253E-5
50-51	2.207979283855167E-5	6.73433681575826E-4	0.0	0.13064613422571025	9.935906777348253E-5
52-53	3.311968925782751E-5	6.73433681575826E-4	0.0	0.157892598588483	1.0487901598312045E-4
54-55	3.311968925782751E-5	6.73433681575826E-4	0.0	0.1905872518341684	1.1591891240239627E-4
56-57	3.311968925782751E-5	6.844735779951019E-4	0.0	0.22627371700947752	1.2143886061203419E-4
58-59	3.311968925782751E-5	6.955134744143776E-4	0.0	0.2585598940876497	1.2143886061203419E-4
60-61	3.311968925782751E-5	7.010334226240155E-4	0.0	0.271559372121347	1.2143886061203419E-4
62-63	3.311968925782751E-5	7.120733190432914E-4	0.0	0.2869158680405597	1.3247875703131004E-4
64-65	3.311968925782751E-5	7.617528529300327E-4	0.0	0.2924744558876651	1.3247875703131004E-4
66-67	3.311968925782751E-5	7.948725421878602E-4	0.0	0.29681865512865013	1.3247875703131004E-4
68-69	3.311968925782751E-5	8.003924903974981E-4	0.0	0.3014995712104231	1.3799870524094796E-4
70-71	3.311968925782751E-5	8.059124386071361E-4	0.0	0.30566713210869967	1.4351865345058585E-4
72-73	3.311968925782751E-5	8.059124386071361E-4	0.0	0.30897910103448245	1.545585498698617E-4
74-75	3.8639637467465425E-5	8.224722832360497E-4	0.0	0.3115072373144966	1.545585498698617E-4
76-77	4.415958567710334E-5	8.335121796553256E-4	0.0	0.31405745338734936	1.545585498698617E-4
78-79	4.415958567710334E-5	8.500720242842393E-4	0.0	0.3157410375912889	1.545585498698617E-4
80-81	4.415958567710334E-5	8.611119207035152E-4	0.0	0.31640343137644544	1.6007849807949962E-4
82-83	4.415958567710334E-5	8.611119207035152E-4	0.0	0.31685054718142613	1.876782391276892E-4
84-85	4.415958567710334E-5	8.77671765332429E-4	0.0	0.3170713451098116	1.876782391276892E-4
86-87	4.415958567710334E-5	8.942316099613427E-4	0.0	0.3171596642811658	1.9319818733732713E-4
88	4.415958567710334E-5	9.163114027998944E-4	0.0	0.31717622412579477	2.0975803196624087E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15515	0.0	55.04529	1
CTTATAC	14210	0.0	43.24485	1
GTATCAA	27710	0.0	35.75511	1
TTATACA	18465	0.0	34.29164	2
TATACAC	20260	0.0	33.475555	3
ATCAACG	34415	0.0	28.2092	3
TCAACGC	34550	0.0	28.12356	4
CAACGCA	35185	0.0	27.688114	5
TATCAAC	35320	0.0	27.606485	2
AACGCAG	36195	0.0	26.995838	6
TGGTATC	6230	0.0	26.244156	2
GTGGTAT	6700	0.0	25.860998	1
TACACAT	27330	0.0	24.831026	5
ACACATC	25745	0.0	24.411674	6
ACGCAGA	40910	0.0	23.84336	7
CGCAGAG	41660	0.0	23.393856	8
CACATCT	27905	0.0	22.461578	7
GTACATG	33460	0.0	21.221216	1
CATCTCC	29570	0.0	21.168346	9
ATACACA	34020	0.0	21.09671	4
>>END_MODULE