FastQCFastQC Report
Fri 27 May 2016
SRR2139549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10649526
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139300.052.1083561
CTTATAC107250.044.1845441
GTATCAA253550.037.0403481
TATACAC158600.032.563423
TTATACA160950.030.8052222
TCAACGC313100.029.3777054
ATCAACG315950.029.1399273
CAACGCA318500.028.9201245
TATCAAC331500.028.1230092
AACGCAG328700.028.0988166
ACACATC193800.024.6941916
ACGCAGA376050.024.5272837
TACACAT209300.024.3671975
ACATCTC198800.023.944468
CGCAGAG385950.023.8873278
CACATCT218150.021.504897
GTACATG306900.021.1435741
TGGTATC66900.021.018792
GCAGAGT439650.020.833039
GTGGTAT72150.020.5621641
Produced by FastQC (version 0.11.5)