##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139549_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10649526
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51636476590601	33.0	33.0	33.0	33.0	33.0
2	32.52439394955231	33.0	33.0	33.0	33.0	33.0
3	32.52073923290107	33.0	33.0	33.0	33.0	33.0
4	36.41383860652577	37.0	37.0	37.0	37.0	37.0
5	36.43752294703069	37.0	37.0	37.0	37.0	37.0
6	36.42159162764615	37.0	37.0	37.0	37.0	37.0
7	36.39712950604562	37.0	37.0	37.0	37.0	37.0
8	36.3805147759628	37.0	37.0	37.0	37.0	37.0
9	36.42843240159233	37.0	37.0	37.0	37.0	37.0
10-11	36.36814441318796	37.0	37.0	37.0	37.0	37.0
12-13	36.34909037266072	37.0	37.0	37.0	37.0	37.0
14-15	38.25367621995571	40.0	37.0	40.0	37.0	40.0
16-17	38.20035056020333	40.0	37.0	40.0	37.0	40.0
18-19	38.20782474262235	40.0	37.0	40.0	35.0	40.0
20-21	38.20472567728008	40.0	37.0	40.0	33.0	40.0
22-23	38.208054464365446	40.0	37.0	40.0	35.0	40.0
24-25	38.18579562979149	40.0	37.0	40.0	35.0	40.0
26-27	38.05006235992515	40.0	37.0	40.0	33.0	40.0
28-29	37.97387146747951	40.0	37.0	40.0	33.0	40.0
30-31	37.90935439850691	40.0	37.0	40.0	33.0	40.0
32-33	37.843946450086975	40.0	37.0	40.0	33.0	40.0
34-35	37.78678188093611	40.0	37.0	40.0	33.0	40.0
36-37	37.73017613315091	40.0	37.0	40.0	33.0	40.0
38-39	37.662628988913305	40.0	37.0	40.0	33.0	40.0
40-41	37.65383524129729	40.0	37.0	40.0	33.0	40.0
42-43	37.59302008003793	40.0	37.0	40.0	33.0	40.0
44-45	37.50435312712256	40.0	37.0	40.0	33.0	40.0
46-47	37.462923982907185	40.0	37.0	40.0	33.0	40.0
48-49	37.46653682574033	40.0	37.0	40.0	33.0	40.0
50-51	37.35876514508293	40.0	37.0	40.0	33.0	40.0
52-53	37.245911150528954	40.0	37.0	40.0	33.0	40.0
54-55	37.11587720508048	38.5	37.0	40.0	33.0	40.0
56-57	36.99805092966618	37.0	37.0	40.0	33.0	40.0
58-59	36.87387974944532	37.0	37.0	40.0	33.0	40.0
60-61	36.71370070564413	37.0	37.0	40.0	33.0	40.0
62-63	36.53797433487398	37.0	37.0	40.0	33.0	40.0
64-65	36.39233967911606	37.0	37.0	40.0	33.0	40.0
66-67	36.174307906813894	37.0	37.0	40.0	33.0	40.0
68-69	35.981784813944884	37.0	37.0	40.0	33.0	40.0
70-71	35.73284017092299	37.0	33.0	37.0	33.0	40.0
72-73	35.519536131911174	37.0	33.0	37.0	33.0	40.0
74-75	35.31790162904845	37.0	33.0	37.0	33.0	40.0
76-77	33.539021625332836	35.0	33.0	37.0	27.0	37.0
78-79	34.79976344642269	37.0	33.0	37.0	30.0	37.0
80-81	34.890059600894055	37.0	33.0	37.0	33.0	37.0
82-83	34.7429131691011	37.0	33.0	37.0	33.0	37.0
84-85	34.57522933937986	37.0	33.0	37.0	30.0	37.0
86-87	34.437308598921945	37.0	33.0	37.0	27.0	37.0
88-89	34.30468146073581	37.0	33.0	37.0	27.0	37.0
90-91	34.23448756184496	37.0	33.0	37.0	27.0	37.0
92-93	34.187687904953464	37.0	33.0	37.0	27.0	37.0
94-95	34.198453178793834	37.0	33.0	37.0	27.0	37.0
96-97	34.161836925905966	37.0	33.0	37.0	27.0	37.0
98-99	34.19120857265235	37.0	33.0	37.0	27.0	37.0
100	34.19732344339553	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	11.0
20	101.0
21	531.0
22	2027.0
23	5768.0
24	13158.0
25	25615.0
26	44710.0
27	70938.0
28	104201.0
29	144720.0
30	195605.0
31	257437.0
32	338493.0
33	446860.0
34	599415.0
35	848334.0
36	1399563.0
37	2978060.0
38	3101447.0
39	72530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.859011001992016	20.566220505964303	14.624491268437675	23.950277223606008
2	14.366226252698947	20.84308728857979	42.35136850222254	22.439317956498723
3	17.894871565175766	26.233383532750658	30.949330514804135	24.92241438726944
4	12.143732178509849	18.510066612441197	38.73814224739473	30.608058961654226
5	13.0902809015037	37.8068823995524	35.79536672067595	13.307469978267944
6	29.445175202196832	38.26921278359484	18.709869098374725	13.575742915833608
7	26.003227937699375	32.86408857334845	22.863960868110162	18.26872262084202
8	24.02451526950589	37.414876493094624	21.062139291457665	17.49846894594182
9	24.978843965938506	16.994380176091823	21.107984604642272	36.918791253327406
10-11	23.9182557380405	26.956516227507898	28.88953002894755	20.235698005504048
12-13	24.82908426765827	24.402501549383064	28.90691963866509	21.861494544293574
14-15	21.95114931479071	25.925993743972963	26.46252109447748	25.66033584675884
16-17	21.535413876636387	29.190440025218024	27.158274462168553	22.11587163597704
18-19	21.49847326538289	27.9909218494795	29.10861948221921	21.401985402918402
20-21	22.301249401423267	27.13102589349388	29.05780090268587	21.509923802396973
22-23	21.936394731657145	27.394281252635423	28.88409089756528	21.785233118142155
24-25	21.79159206654539	27.608832892315544	29.14110161487561	21.45847342626346
26-27	21.905833818327576	27.696533542402342	29.080674540705598	21.316958098564488
28-29	21.5976605463598	27.395360116203683	29.330237714688508	21.67674162274801
30-31	21.98946006290422	27.478614206108148	29.03190895633976	21.500016774647875
32-33	21.826147358466862	27.35549226616836	29.140287555275744	21.678072820089035
34-35	21.615783174673595	27.746389645435343	29.366806768873133	21.27102041101793
36-37	21.964096247527788	27.486779058069715	28.84191197217596	21.707212722226537
38-39	21.628317912800654	27.438721833779955	29.115194347501966	21.817765905917426
40-41	21.82997876345187	27.332263722146337	29.101658328441527	21.736099185960263
42-43	21.911787748333715	27.315340090540225	28.97668807949994	21.796184081626123
44-45	21.74660202670196	27.697617903768325	28.692279248879508	21.86350082065021
46-47	22.166505039698187	27.440344577148522	28.108339100132678	22.284811283020613
48-49	22.007782312254182	27.49876089610021	28.249236194163263	22.24422059748234
50-51	21.840969984370762	27.827724519686253	28.229268707974626	22.102036787968355
52-53	21.98011910064631	27.874417914373563	28.26819094592954	21.87727203905059
54-55	22.003137151492613	27.847629246464667	28.01661975700915	22.13261384503357
56-57	22.205348935587732	28.067011007536458	27.712688899831832	22.01495115704397
58-59	21.86332185347847	28.243906566846704	28.10912281125404	21.78364876842079
60-61	22.15127977364509	28.4330567535862	27.634648482457724	21.78101499031098
62-63	21.938385045396846	28.612242779787856	27.62185650826937	21.827515666545924
64-65	21.9782398240575	28.331484407185265	27.701748475850312	21.988527292906923
66-67	22.101559166743787	28.203716554575344	27.581858309107943	22.112865969572926
68-69	22.076438785554085	28.071794805395893	27.739293145321998	22.112473263728024
70-71	22.07963221378512	27.853508156486484	27.808233896084733	22.25862573364366
72-73	22.130471681993587	27.939655940548175	27.714499491446297	22.215372886011938
74-75	22.04028140180225	28.080250967666583	27.68005786167326	22.19940976885791
76-77	22.121943391842215	28.03662429948818	27.63656612045225	22.204866188217352
78-79	22.096987691595192	27.988445284598463	27.61154491855256	22.303022105253785
80-81	22.087265280015814	28.024251368302	27.648014294975955	22.24046905670623
82-83	22.169393923046897	28.00633607154498	27.69884824662128	22.12542175878684
84-85	22.264568445661613	27.966552670699237	27.64717034139675	22.12170854224241
86-87	22.06235060423058	28.024240083906292	27.732950070768243	22.18045924109488
88-89	22.161529719227982	27.995356997288628	27.668817172208023	22.17429611127537
90-91	22.158482089834898	28.05234171357092	27.678879878558888	22.11029631803529
92-93	22.163682132162585	28.01730473270421	27.72204459502989	22.096968540103315
94-95	22.011334445463923	28.07020110732608	27.74424027437929	22.174224172830705
96-97	22.08535107074297	28.178397629778758	27.836493745458412	21.899757554019857
98-99	23.15466252442752	30.084735514689914	28.05141721367607	18.709184747206496
100	22.152503793095484	31.091746350261474	21.910473844619965	24.845276012023078
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3562.0
1	2879.5
2	3169.0
3	4423.5
4	5918.5
5	9526.0
6	12731.0
7	15146.0
8	16817.5
9	16722.0
10	15137.0
11	13490.5
12	14513.0
13	19187.0
14	30303.5
15	45733.0
16	58540.0
17	66101.0
18	68350.5
19	65666.0
20	60194.0
21	54204.5
22	50543.0
23	52440.0
24	59881.5
25	72526.5
26	89931.5
27	110322.0
28	130594.0
29	151854.0
30	173488.0
31	195797.0
32	221388.5
33	247533.5
34	273566.0
35	296917.5
36	321335.5
37	343848.0
38	359337.5
39	371909.0
40	384815.5
41	394565.0
42	399157.0
43	407187.5
44	415164.0
45	421071.0
46	431101.0
47	445204.0
48	449307.0
49	445786.0
50	442982.0
51	427744.5
52	401570.0
53	372005.0
54	343210.0
55	317068.5
56	289030.0
57	257643.0
58	227023.0
59	198938.5
60	166777.5
61	133327.0
62	104743.5
63	80044.5
64	59200.5
65	42790.5
66	31258.5
67	23322.0
68	17580.0
69	12996.5
70	9451.5
71	6758.5
72	4628.0
73	3094.0
74	2038.5
75	1330.5
76	890.5
77	578.5
78	402.0
79	299.5
80	215.0
81	148.5
82	107.0
83	78.0
84	64.0
85	53.0
86	42.5
87	32.5
88	22.5
89	14.5
90	9.5
91	7.5
92	6.0
93	3.5
94	2.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0015399746430028905
5	0.006948666072086213
6	1.878017857320598E-5
7	1.878017857320598E-5
8	0.0
9	7.699873215014452E-4
10-11	8.779733482973795E-4
12-13	0.0011831512501119768
14-15	0.0010845553126026454
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	176.0
20-21	802.0
22-23	1612.0
24-25	3150.0
26-27	4783.0
28-29	8520.0
30-31	18700.0
32-33	23694.0
34-35	33591.0
36-37	46795.0
38-39	55291.0
40-41	58634.0
42-43	70853.0
44-45	84360.0
46-47	81225.0
48-49	74276.0
50-51	64768.0
52-53	64572.0
54-55	76485.0
56-57	83884.0
58-59	82082.0
60-61	85041.0
62-63	83609.0
64-65	90234.0
66-67	88498.0
68-69	74862.0
70-71	74086.0
72-73	70617.0
74-75	72319.0
76-77	70398.0
78-79	77855.0
80-81	80372.0
82-83	78219.0
84-85	79310.0
86-87	85790.0
88-89	92697.0
90-91	99072.0
92-93	107525.0
94-95	134628.0
96-97	359978.0
98-99	664898.0
100-101	7141265.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.83828416415547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.48234333468527	73.29725500669026
2	7.843594167291645	12.994997649968354
3	1.9983099404331115	4.966097000809641
4	0.7404531556706155	2.453514756787524
5	0.3463759651741185	1.4346595315363624
6	0.18873179792932937	0.9380530984609052
7	0.11547932925864465	0.6696276644549576
8	0.06793420466846177	0.4502042360633547
9	0.050794547471651685	0.3786959841701731
>10	0.1619763828431719	2.090747023590918
>50	0.003025716449056658	0.17150878968785335
>100	8.995640704561904E-4	0.10296295423990849
>500	6.142131928941776E-5	0.030697695814646694
>1k	2.0472735138469155E-5	0.020978607725092645
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.390089286602991E-6
2	0.0	2.8170267859808972E-5	0.0	0.0	9.390089286602991E-6
3	0.0	3.7560357146411965E-5	0.0	0.0	9.390089286602991E-6
4	0.0	1.1268107143923589E-4	0.0	0.0	1.8780178573205983E-5
5	0.0	1.1268107143923589E-4	0.0	0.0	1.8780178573205983E-5
6	0.0	1.1268107143923589E-4	0.0	0.0	2.8170267859808972E-5
7	9.390089286602991E-6	1.2207116072583888E-4	0.0	0.0	2.8170267859808972E-5
8	9.390089286602991E-6	1.3146125001244188E-4	0.0	9.390089286602991E-6	3.7560357146411965E-5
9	1.8780178573205983E-5	2.065819643052658E-4	0.0	1.8780178573205983E-5	4.6950446433014955E-5
10-11	3.7560357146411965E-5	2.065819643052658E-4	0.0	4.6950446433014955E-5	4.6950446433014955E-5
12-13	3.7560357146411965E-5	2.065819643052658E-4	0.0	7.981575893612543E-5	4.6950446433014955E-5
14-15	3.7560357146411965E-5	2.6292250002488376E-4	0.0	1.1268107143923589E-4	6.103558036291944E-5
16-17	3.7560357146411965E-5	2.6292250002488376E-4	0.0	1.3146125001244188E-4	7.512071429282393E-5
18-19	3.7560357146411965E-5	3.004828571712957E-4	0.0	1.4554638394234637E-4	7.512071429282393E-5
20-21	3.7560357146411965E-5	3.4743330360431066E-4	0.0	1.7371665180215533E-4	7.512071429282393E-5
22-23	4.225540178971346E-5	4.084688839672301E-4	0.0	1.924968303753613E-4	7.981575893612543E-5
24-25	4.6950446433014955E-5	4.5072428575694356E-4	0.0	2.065819643052658E-4	8.451080357942692E-5
26-27	5.6340535719617944E-5	4.6011437504354653E-4	0.0	2.3005718752177327E-4	8.920584822272841E-5
28-29	5.6340535719617944E-5	4.6011437504354653E-4	0.0	3.990787946806271E-4	9.390089286602991E-5
30-31	5.6340535719617944E-5	5.258450000497675E-4	0.0	9.296188393736961E-4	9.390089286602991E-5
32-33	5.6340535719617944E-5	5.258450000497675E-4	0.0	0.0020892948662691656	9.390089286602991E-5
34-35	5.6340535719617944E-5	6.009657143425914E-4	0.0	0.00373725553606799	1.1268107143923589E-4
36-37	5.6340535719617944E-5	6.854765179220183E-4	0.0	0.006141118393438356	1.1268107143923589E-4
38-39	5.6340535719617944E-5	6.948666072086213E-4	0.0	0.009526245581258735	1.1268107143923589E-4
40-41	5.6340535719617944E-5	7.418170536416362E-4	0.0	0.012925457903009016	1.1268107143923589E-4
42-43	6.573062500622094E-5	7.559021875715407E-4	0.0	0.016381010760478917	1.2676620536914037E-4
44-45	6.573062500622094E-5	8.451080357942693E-4	0.0	0.019761442903655994	1.4554638394234637E-4
46-47	1.032909821526329E-4	9.108386608004901E-4	0.0	0.02367711013616944	1.6432656251555233E-4
48-49	1.1268107143923589E-4	9.108386608004901E-4	0.0	0.027653812949045807	1.7841169644545682E-4
50-51	1.1268107143923589E-4	0.001023519732239726	0.0	0.031499054511909735	1.7841169644545682E-4
52-53	1.1268107143923589E-4	0.001042299910812932	0.0	0.035471062280142795	2.0188691966196428E-4
54-55	1.1268107143923589E-4	0.001051690000099535	0.0	0.03921770790549739	2.3475223216507478E-4
56-57	1.3615629465574337E-4	0.001070470178672741	0.0	0.04315215531658404	2.4414232145167776E-4
58-59	1.6902160715885384E-4	0.0011221156697490573	0.0	0.04753732701342764	2.4414232145167776E-4
60-61	1.7841169644545682E-4	0.0011268107143923589	0.0	0.051772257281685594	2.5353241073828073E-4
62-63	1.8780178573205982E-4	0.0011408958483222634	0.0	0.05602596772851674	2.6292250002488376E-4
64-65	1.8780178573205982E-4	0.0012488818751181978	0.0	0.06023742277355819	2.6292250002488376E-4
66-67	1.9719187501866282E-4	0.0012488818751181978	0.0	0.06458972915789868	2.6292250002488376E-4
68-69	2.065819643052658E-4	0.001300527366194514	0.0	0.06864155268506786	2.6292250002488376E-4
70-71	2.065819643052658E-4	0.0013286976340543231	0.0	0.07227551723898322	2.6292250002488376E-4
72-73	2.065819643052658E-4	0.0013521728572708307	0.0	0.07546814759642824	2.910927678846927E-4
74-75	2.112770089485673E-4	0.00141320843763375	0.0	0.07799877665916774	2.910927678846927E-4
76-77	2.1597205359186877E-4	0.001488329151926574	0.0	0.08059983139155677	2.910927678846927E-4
78-79	2.1597205359186877E-4	0.0015118043751430816	0.0	0.08208816054348335	2.910927678846927E-4
80-81	2.5353241073828073E-4	0.0015118043751430816	0.0	0.0826233956328197	3.051779018145972E-4
82-83	2.6292250002488376E-4	0.0015211944644296844	0.0	0.08306003478464674	3.2865312503110465E-4
84-85	2.7231258931148674E-4	0.0015305845537162874	0.0	0.08323844648109222	3.2865312503110465E-4
86-87	2.910927678846927E-4	0.0015587548215760965	0.0	0.08332765232931494	3.2865312503110465E-4
88	2.910927678846927E-4	0.0016526557144421264	0.0	0.08333704241860154	3.2865312503110465E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13930	0.0	52.108356	1
CTTATAC	10725	0.0	44.184544	1
GTATCAA	25355	0.0	37.040348	1
TATACAC	15860	0.0	32.56342	3
TTATACA	16095	0.0	30.805222	2
TCAACGC	31310	0.0	29.377705	4
ATCAACG	31595	0.0	29.139927	3
CAACGCA	31850	0.0	28.920124	5
TATCAAC	33150	0.0	28.123009	2
AACGCAG	32870	0.0	28.098816	6
ACACATC	19380	0.0	24.694191	6
ACGCAGA	37605	0.0	24.527283	7
TACACAT	20930	0.0	24.367197	5
ACATCTC	19880	0.0	23.94446	8
CGCAGAG	38595	0.0	23.887327	8
CACATCT	21815	0.0	21.50489	7
GTACATG	30690	0.0	21.143574	1
TGGTATC	6690	0.0	21.01879	2
GCAGAGT	43965	0.0	20.83303	9
GTGGTAT	7215	0.0	20.562164	1
>>END_MODULE