FastQCFastQC Report
Fri 27 May 2016
SRR2139548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3830786
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66950.053.8608781
CTTATAC35950.037.420321
GTATCAA113050.034.06431
TTATACA50250.028.1398542
TATACAC53350.027.9548443
TCAACGC137850.027.437084
TGGTATC29150.027.4243532
GTGGTAT29450.027.2909281
ATCAACG139050.027.1384813
CAACGCA140400.026.9081465
TATCAAC141800.026.854652
AACGCAG144350.026.1701226
ACGCAGA165650.022.787
CGCAGAG169500.022.262878
GTACATG130550.022.1893751
ACATGGG129500.021.8714543
TACATGG131950.021.6933612
TACACAT71550.020.9641785
GAGTACT119100.020.80358712-13
ACATCTC65900.020.6742698
Produced by FastQC (version 0.11.5)