##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139548_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3830786
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50354496440156	33.0	33.0	33.0	33.0	33.0
2	32.51145091372893	33.0	33.0	33.0	33.0	33.0
3	32.50398273356956	33.0	33.0	33.0	33.0	33.0
4	36.39721195597979	37.0	37.0	37.0	37.0	37.0
5	36.422319074988785	37.0	37.0	37.0	37.0	37.0
6	36.405952720929854	37.0	37.0	37.0	37.0	37.0
7	36.37777338645385	37.0	37.0	37.0	37.0	37.0
8	36.365219565906315	37.0	37.0	37.0	37.0	37.0
9	36.41302489880667	37.0	37.0	37.0	37.0	37.0
10-11	36.3527303535097	37.0	37.0	37.0	37.0	37.0
12-13	36.333499443717294	37.0	37.0	37.0	37.0	37.0
14-15	38.207495015383266	40.0	37.0	40.0	35.0	40.0
16-17	38.14647946922642	40.0	37.0	40.0	33.0	40.0
18-19	38.16635058705968	40.0	37.0	40.0	33.0	40.0
20-21	38.16346363402788	40.0	37.0	40.0	33.0	40.0
22-23	38.1692448349735	40.0	37.0	40.0	33.0	40.0
24-25	38.14786073029278	40.0	37.0	40.0	33.0	40.0
26-27	38.00427881766613	40.0	37.0	40.0	33.0	40.0
28-29	37.92581797866268	40.0	37.0	40.0	33.0	40.0
30-31	37.84896718204925	40.0	37.0	40.0	33.0	40.0
32-33	37.779301219679965	40.0	37.0	40.0	33.0	40.0
34-35	37.72895503312466	40.0	37.0	40.0	33.0	40.0
36-37	37.65812814034176	40.0	37.0	40.0	33.0	40.0
38-39	37.59114278633104	40.0	37.0	40.0	33.0	40.0
40-41	37.563508345600866	40.0	37.0	40.0	33.0	40.0
42-43	37.50124725396889	40.0	37.0	40.0	33.0	40.0
44-45	37.409167082946205	40.0	37.0	40.0	33.0	40.0
46-47	37.35469423460491	40.0	37.0	40.0	33.0	40.0
48-49	37.3570789220187	40.0	37.0	40.0	33.0	40.0
50-51	37.23941142690066	40.0	37.0	40.0	33.0	40.0
52-53	37.12748172281914	40.0	37.0	40.0	33.0	40.0
54-55	37.00036541703971	37.0	37.0	40.0	33.0	40.0
56-57	36.88524962345163	37.0	37.0	40.0	33.0	40.0
58-59	36.76377225327977	37.0	37.0	40.0	33.0	40.0
60-61	36.60047792840028	37.0	37.0	40.0	33.0	40.0
62-63	36.4140771509964	37.0	37.0	40.0	33.0	40.0
64-65	36.264361197263796	37.0	37.0	40.0	33.0	40.0
66-67	36.043575270435696	37.0	37.0	40.0	33.0	40.0
68-69	35.846764350140724	37.0	33.0	38.5	33.0	40.0
70-71	35.596372993856576	37.0	33.0	37.0	33.0	40.0
72-73	35.389329186466625	37.0	33.0	37.0	33.0	40.0
74-75	35.18924899580008	37.0	33.0	37.0	33.0	40.0
76-77	33.4142741950777	35.0	33.0	37.0	27.0	37.0
78-79	34.691125691824595	37.0	33.0	37.0	30.0	37.0
80-81	34.793331611336285	37.0	33.0	37.0	33.0	37.0
82-83	34.655928680539546	37.0	33.0	37.0	30.0	37.0
84-85	34.493441637028155	37.0	33.0	37.0	27.0	37.0
86-87	34.35911861914326	37.0	33.0	37.0	27.0	37.0
88-89	34.22912622501759	37.0	33.0	37.0	27.0	37.0
90-91	34.165923650250775	37.0	33.0	37.0	27.0	37.0
92-93	34.11787884469259	37.0	33.0	37.0	27.0	37.0
94-95	34.12976170414154	37.0	33.0	37.0	27.0	37.0
96-97	34.095394193321994	37.0	33.0	37.0	27.0	37.0
98-99	34.12672351082852	37.0	33.0	37.0	27.0	37.0
100	34.138494749686345	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	0.0
18	0.0
19	5.0
20	33.0
21	215.0
22	858.0
23	2278.0
24	5103.0
25	9893.0
26	16979.0
27	26828.0
28	39706.0
29	54814.0
30	74110.0
31	97654.0
32	126949.0
33	166298.0
34	222871.0
35	313535.0
36	518077.0
37	1083888.0
38	1047045.0
39	23644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.52398489500588	20.249160355081177	14.816358836019553	23.41049591389339
2	14.831342706170483	20.602717040314964	41.904429012740465	22.661511240774086
3	18.22779972569598	25.722397440107592	30.959364475071173	25.090438359125255
4	12.421389188874523	18.159916402553137	37.99034598807175	31.428348420500583
5	13.163994654496308	37.73159128460474	35.54749512401615	13.5569189368828
6	30.067589262360254	37.646399459536504	18.246673137053335	14.039338141049903
7	26.42565427190511	32.90631032542938	22.27431192301317	18.393723479652344
8	24.514864573484395	37.146789196786244	20.525761553895205	17.81258467583415
9	25.003080335140464	16.85342721220272	20.87219428456867	37.271298168088144
10-11	24.250241760657147	26.75619914264864	28.363873250457516	20.629685846236697
12-13	25.10617058742221	24.361602721976702	28.558866674907012	21.973360015694073
14-15	22.34218578545983	25.916423861511067	26.104598672869635	25.636791680159465
16-17	21.683526566088528	29.136004986966118	26.98956558784542	22.190902859099936
18-19	21.755561391317606	27.671605774898417	29.046454174156427	21.526378659627554
20-21	22.380868657432952	26.89629436160136	29.22427623301669	21.498560747949004
22-23	22.117560350168436	26.934112494912238	29.202124340549275	21.74620281437005
24-25	22.017381249756824	27.043051508423098	29.14703087507628	21.792536366743796
26-27	21.940293127079812	27.194123352443427	29.28158344106543	21.584000079411332
28-29	21.70110120114877	26.86084489195516	29.4969503429688	21.94110356392727
30-31	21.82238839750022	26.93626396053308	29.46742945654492	21.77391818542178
32-33	21.490699088114027	27.03812557325456	29.63566502509541	21.835510313536002
34-35	21.714084739641272	27.110342155578437	29.34356121852271	21.832011886257586
36-37	21.845114615110983	27.01733063910275	29.245028282301433	21.892526463484835
38-39	21.735672038068373	27.097978054840116	29.26722039101572	21.899129516075792
40-41	21.83768684041638	26.974736432849294	29.33798642419465	21.84959030253967
42-43	21.889819327737843	27.04981784932033	29.185876720334292	21.87448610260753
44-45	21.844746101616543	27.326317146844346	28.847619425297495	21.981317326241612
46-47	22.284942301759052	27.13396700641164	28.22587227540619	22.355218416423117
48-49	22.036563994945453	27.28689540123544	28.31515318746611	22.361387416352997
50-51	22.000026247788796	27.57726692826963	28.271327537176884	22.15137928676469
52-53	22.151704124801324	27.644095037210704	28.2420323446343	21.962168493353673
54-55	22.1142614339014	27.577957512405472	28.055400449754746	22.25238060393838
56-57	22.27083653942859	27.73374074193711	27.809459837071355	22.18596288156295
58-59	22.054810944439396	27.815787007229364	28.06500524022473	22.064396808106512
60-61	22.196144934137795	28.06254514204218	27.750975412323616	21.990334511496414
62-63	22.101176475011275	28.1781533762726	27.795109620066576	21.925560528649548
64-65	22.141445432803476	27.954837441733115	27.755113168826277	22.14860395663713
66-67	22.277377697050486	27.829404498903486	27.616119660798244	22.277098143247784
68-69	22.202376022108993	27.744572131066263	27.74296899747447	22.31008284935027
70-71	22.223228460667734	27.582146728725466	27.84540365413246	22.349221156474336
72-73	22.29455235524105	27.633035343179674	27.721158608899017	22.35125369268026
74-75	22.08242109905651	27.78746618628794	27.75548759216856	22.37462512248699
76-77	22.33329565214857	27.61920918287916	27.68162088198207	22.365874282990198
78-79	22.25635307770151	27.596786962721332	27.615086449995374	22.531773509581786
80-81	22.270106327697167	27.668294934444575	27.627994213934524	22.43360452392373
82-83	22.274730520344498	27.611401950014947	27.72779181175331	22.386075717887245
84-85	22.29755930448237	27.62400346368123	27.702623681249676	22.375813550586724
86-87	22.280422778006198	27.610007325011516	27.75025743583465	22.359312461147635
88-89	22.3400478571004	27.52718717894833	27.72592049562988	22.406844468321385
90-91	22.331073755252397	27.562353488147284	27.711366286577714	22.3952064700226
92-93	22.341254161916456	27.476477717713955	27.773567091392586	22.408701028977
94-95	22.205401850965465	27.492465046245414	27.85445032387756	22.447682778911556
96-97	22.381818416779023	27.607266085712933	27.83832657755646	22.172588919951583
98-99	23.38704121342455	29.492831242418475	28.074053729787195	19.04607381436978
100	22.42895811759404	30.425568622602974	21.971587550413066	25.173885709389925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1418.0
1	1132.0
2	1265.0
3	1728.5
4	2136.5
5	3420.5
6	4801.0
7	6380.5
8	7808.5
9	8222.0
10	7321.5
11	5967.5
12	6144.5
13	7950.5
14	12476.5
15	18954.5
16	23889.0
17	26419.0
18	27299.0
19	26363.0
20	23870.0
21	20991.5
22	18781.0
23	18475.0
24	20553.5
25	24711.0
26	30485.5
27	36914.0
28	43792.5
29	51917.5
30	59955.0
31	67714.0
32	76835.5
33	85837.0
34	94003.5
35	101523.5
36	110607.5
37	118484.0
38	124087.5
39	129025.5
40	132512.0
41	135930.0
42	137842.0
43	140751.5
44	144227.5
45	146300.5
46	147514.0
47	148039.0
48	152483.0
49	155094.5
50	152671.5
51	150653.5
52	148566.0
53	142690.0
54	132466.0
55	122312.0
56	112348.5
57	101617.0
58	90867.5
59	79489.0
60	67082.5
61	55018.0
62	43661.0
63	33252.5
64	24629.5
65	18007.5
66	13203.0
67	9843.5
68	7459.0
69	5530.0
70	3946.0
71	2736.0
72	1823.0
73	1201.5
74	769.5
75	512.0
76	356.0
77	232.5
78	151.0
79	102.5
80	80.0
81	66.5
82	50.5
83	37.5
84	27.5
85	19.0
86	14.5
87	10.0
88	7.5
89	6.0
90	4.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.001226902259745128
5	0.007230892041476605
6	0.0
7	2.610430339883251E-5
8	0.0
9	8.875463155603053E-4
10-11	9.005984672597217E-4
12-13	0.0010572242876527166
14-15	0.0010311199842538842
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	88.0
20-21	298.0
22-23	587.0
24-25	1236.0
26-27	1886.0
28-29	3206.0
30-31	6781.0
32-33	8342.0
34-35	12086.0
36-37	16771.0
38-39	19401.0
40-41	20504.0
42-43	25328.0
44-45	31064.0
46-47	30256.0
48-49	27582.0
50-51	23066.0
52-53	22148.0
54-55	27642.0
56-57	29804.0
58-59	29230.0
60-61	28851.0
62-63	27951.0
64-65	30128.0
66-67	31157.0
68-69	27953.0
70-71	27747.0
72-73	26560.0
74-75	26939.0
76-77	26619.0
78-79	29684.0
80-81	30857.0
82-83	29276.0
84-85	30045.0
86-87	32153.0
88-89	36266.0
90-91	39589.0
92-93	41394.0
94-95	48539.0
96-97	129330.0
98-99	236309.0
100-101	2556133.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.74572791867837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.4945311493407	83.00989026494453
2	5.755765780625945	10.33110779423391
3	1.0539739496452722	2.837689779547183
4	0.34628368000150556	1.2430992371237521
5	0.1440908890126593	0.6465770860445302
6	0.06443328299759749	0.34695671308857534
7	0.03987351497047299	0.2504934337990993
8	0.02721527048559464	0.1953963408186732
9	0.016127386356169324	0.13026276251641603
>10	0.05576074935439354	0.7686009315900504
>50	0.0010657524468603045	0.06122895711155875
>100	8.388353606723199E-4	0.16064142255321057
>500	3.975940215298732E-5	0.018055276628426455
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.6104303398832512E-5	0.0	7.831291019649754E-5	0.0
3	0.0	2.6104303398832512E-5	0.0	7.831291019649754E-5	0.0
4	0.0	5.2208606797665024E-5	0.0	7.831291019649754E-5	0.0
5	0.0	7.831291019649754E-5	0.0	7.831291019649754E-5	0.0
6	0.0	7.831291019649754E-5	0.0	7.831291019649754E-5	0.0
7	0.0	7.831291019649754E-5	0.0	7.831291019649754E-5	0.0
8	0.0	7.831291019649754E-5	0.0	7.831291019649754E-5	0.0
9	0.0	1.0441721359533005E-4	0.0	1.0441721359533005E-4	0.0
10-11	2.6104303398832512E-5	1.0441721359533005E-4	0.0	1.174693652947463E-4	2.6104303398832512E-5
12-13	2.6104303398832512E-5	1.0441721359533005E-4	0.0	1.5662582039299507E-4	2.6104303398832512E-5
14-15	2.6104303398832512E-5	1.3052151699416255E-4	0.0	1.8273012379182757E-4	3.915645509824877E-5
16-17	5.2208606797665024E-5	1.3052151699416255E-4	0.0	2.088344271906601E-4	5.2208606797665024E-5
18-19	5.2208606797665024E-5	1.435736686935788E-4	0.0	2.2188657889007636E-4	1.0441721359533005E-4
20-21	5.2208606797665024E-5	1.5662582039299507E-4	0.0	2.349387305894926E-4	1.174693652947463E-4
22-23	5.2208606797665024E-5	1.8273012379182757E-4	0.0	2.871473373871576E-4	1.3052151699416255E-4
24-25	5.2208606797665024E-5	2.088344271906601E-4	0.0	3.524080958842389E-4	1.3052151699416255E-4
26-27	5.2208606797665024E-5	2.349387305894926E-4	0.0	4.959817645778177E-4	1.3052151699416255E-4
28-29	5.2208606797665024E-5	2.349387305894926E-4	0.0	9.528070740573867E-4	1.5662582039299507E-4
30-31	5.2208606797665024E-5	2.610430339883251E-4	0.0	0.0020622399685077683	1.5662582039299507E-4
32-33	5.2208606797665024E-5	2.610430339883251E-4	0.0	0.004137532088714953	2.088344271906601E-4
34-35	5.2208606797665024E-5	3.393559441848226E-4	0.0	0.006995953310887112	2.088344271906601E-4
36-37	7.831291019649754E-5	4.176688543813202E-4	0.0	0.01195577095666529	2.088344271906601E-4
38-39	1.0441721359533005E-4	4.4377315778015267E-4	0.0	0.02017862652729753	2.088344271906601E-4
40-41	1.0441721359533005E-4	4.698774611789852E-4	0.0	0.02622177276412726	2.088344271906601E-4
42-43	1.0441721359533005E-4	4.959817645778177E-4	0.0	0.030750869403824696	2.088344271906601E-4
44-45	1.0441721359533005E-4	6.003989781731478E-4	0.0	0.03624582526927894	2.088344271906601E-4
46-47	1.0441721359533005E-4	6.787118883696452E-4	0.0	0.04180604189323027	2.088344271906601E-4
48-49	1.0441721359533005E-4	6.787118883696452E-4	0.0	0.04782308382666116	2.088344271906601E-4
50-51	1.0441721359533005E-4	7.309204951673103E-4	0.0	0.052273867556162104	2.088344271906601E-4
52-53	1.0441721359533005E-4	7.309204951673103E-4	0.0	0.055510801177617336	2.2188657889007636E-4
54-55	1.0441721359533005E-4	7.309204951673103E-4	0.0	0.05899572568136148	2.349387305894926E-4
56-57	1.0441721359533005E-4	7.309204951673103E-4	0.0	0.06284611043268927	2.7409518568774136E-4
58-59	1.0441721359533005E-4	7.309204951673103E-4	0.0	0.06685312100441006	3.1325164078599015E-4
60-61	1.0441721359533005E-4	7.309204951673103E-4	0.0	0.07087318372783027	3.1325164078599015E-4
62-63	1.0441721359533005E-4	7.831291019649753E-4	0.0	0.07476272493425631	3.393559441848226E-4
64-65	1.0441721359533005E-4	8.614420121614729E-4	0.0	0.07833901449989636	3.393559441848226E-4
66-67	1.0441721359533005E-4	8.614420121614729E-4	0.0	0.08186309545873875	3.393559441848226E-4
68-69	1.0441721359533005E-4	8.614420121614729E-4	0.0	0.08630082703654028	3.393559441848226E-4
70-71	1.0441721359533005E-4	8.614420121614729E-4	0.0	0.089929325208978	3.393559441848226E-4
72-73	1.0441721359533005E-4	8.614420121614729E-4	0.0	0.09265722491415601	3.6546024758365514E-4
74-75	1.0441721359533005E-4	8.614420121614729E-4	0.0	0.09477167348946144	3.6546024758365514E-4
76-77	1.0441721359533005E-4	8.614420121614729E-4	0.0	0.09712106079535635	3.6546024758365514E-4
78-79	1.0441721359533005E-4	8.875463155603053E-4	0.0	0.09871342330268515	3.6546024758365514E-4
80-81	1.0441721359533005E-4	9.397549223579704E-4	0.0	0.09930077012915887	3.6546024758365514E-4
82-83	1.0441721359533005E-4	9.397549223579704E-4	0.0	0.09975759543863844	3.6546024758365514E-4
84-85	1.0441721359533005E-4	9.397549223579704E-4	0.0	0.0999664298658291	3.6546024758365514E-4
86-87	1.174693652947463E-4	9.397549223579704E-4	0.0	0.10005779492772501	3.6546024758365514E-4
88	1.3052151699416255E-4	0.0010441721359533004	0.0	0.10005779492772501	3.6546024758365514E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6695	0.0	53.860878	1
CTTATAC	3595	0.0	37.42032	1
GTATCAA	11305	0.0	34.0643	1
TTATACA	5025	0.0	28.139854	2
TATACAC	5335	0.0	27.954844	3
TCAACGC	13785	0.0	27.43708	4
TGGTATC	2915	0.0	27.424353	2
GTGGTAT	2945	0.0	27.290928	1
ATCAACG	13905	0.0	27.138481	3
CAACGCA	14040	0.0	26.908146	5
TATCAAC	14180	0.0	26.85465	2
AACGCAG	14435	0.0	26.170122	6
ACGCAGA	16565	0.0	22.78	7
CGCAGAG	16950	0.0	22.26287	8
GTACATG	13055	0.0	22.189375	1
ACATGGG	12950	0.0	21.871454	3
TACATGG	13195	0.0	21.693361	2
TACACAT	7155	0.0	20.964178	5
GAGTACT	11910	0.0	20.803587	12-13
ACATCTC	6590	0.0	20.674269	8
>>END_MODULE