FastQCFastQC Report
Fri 27 May 2016
SRR2139547_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139547_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6344446
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98800.054.712091
CTTATAC68050.048.002541
GTATCAA172100.035.935731
TTATACA96300.035.127472
TATACAC103200.034.5720753
TCAACGC215700.027.9935594
ATCAACG217450.027.7880633
TATCAAC222700.027.345562
CAACGCA221000.027.3029615
GTGGTAT43650.027.114341
AACGCAG226000.026.7729786
TGGTATC42900.025.9832272
TACACAT142850.025.3679775
ACGCAGA255600.023.6223687
ACACATC146350.023.2890746
ACATCTC147450.023.1742538
CGCAGAG261700.023.0883798
ATACACA179400.021.8547844
GTACATG198500.021.3346331
CACATCT159650.021.0527447
Produced by FastQC (version 0.11.5)