##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139547_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6344446
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46909785346112	33.0	33.0	33.0	33.0	33.0
2	32.486476518201904	33.0	33.0	33.0	33.0	33.0
3	32.47945778086849	33.0	33.0	33.0	33.0	33.0
4	36.364901048885905	37.0	37.0	37.0	37.0	37.0
5	36.386576700313945	37.0	37.0	37.0	37.0	37.0
6	36.37487654556442	37.0	37.0	37.0	37.0	37.0
7	36.34718334745067	37.0	37.0	37.0	37.0	37.0
8	36.33069995394397	37.0	37.0	37.0	33.0	37.0
9	36.38484778655221	37.0	37.0	37.0	37.0	37.0
10-11	36.319827925085974	37.0	37.0	37.0	35.0	37.0
12-13	36.29937728211415	37.0	37.0	37.0	33.0	37.0
14-15	38.144189185312634	40.0	37.0	40.0	33.0	40.0
16-17	38.0771626080512	40.0	37.0	40.0	33.0	40.0
18-19	38.09660094829398	40.0	37.0	40.0	33.0	40.0
20-21	38.102122089582174	40.0	37.0	40.0	33.0	40.0
22-23	38.10344459308845	40.0	37.0	40.0	33.0	40.0
24-25	38.07778922587528	40.0	37.0	40.0	33.0	40.0
26-27	37.936207892669145	40.0	37.0	40.0	33.0	40.0
28-29	37.85287378625401	40.0	37.0	40.0	33.0	40.0
30-31	37.78639483566961	40.0	37.0	40.0	33.0	40.0
32-33	37.7005917732085	40.0	37.0	40.0	33.0	40.0
34-35	37.62721211617141	40.0	37.0	40.0	33.0	40.0
36-37	37.571805467453856	40.0	37.0	40.0	33.0	40.0
38-39	37.5014566688369	40.0	37.0	40.0	33.0	40.0
40-41	37.48169254125278	40.0	37.0	40.0	33.0	40.0
42-43	37.41351280489771	40.0	37.0	40.0	33.0	40.0
44-45	37.31914979134381	40.0	37.0	40.0	33.0	40.0
46-47	37.26880935616791	40.0	37.0	40.0	33.0	40.0
48-49	37.2771416620427	40.0	37.0	40.0	33.0	40.0
50-51	37.15547735986917	40.0	37.0	40.0	33.0	40.0
52-53	37.045207971777316	38.5	37.0	40.0	33.0	40.0
54-55	36.90799462509159	37.0	37.0	40.0	33.0	40.0
56-57	36.79334194035308	37.0	37.0	40.0	33.0	40.0
58-59	36.669198273426346	37.0	37.0	40.0	33.0	40.0
60-61	36.50574142143735	37.0	37.0	40.0	33.0	40.0
62-63	36.32897139430819	37.0	37.0	40.0	33.0	40.0
64-65	36.18025218773876	37.0	37.0	40.0	33.0	40.0
66-67	35.96440288258859	37.0	35.0	40.0	33.0	40.0
68-69	35.77097569838587	37.0	33.0	38.5	33.0	40.0
70-71	35.51826674161127	37.0	33.0	37.0	33.0	40.0
72-73	35.30582605872738	37.0	33.0	37.0	33.0	40.0
74-75	35.108774201675274	37.0	33.0	37.0	30.0	40.0
76-77	33.33981888421161	35.0	33.0	37.0	27.0	37.0
78-79	34.62253338724639	37.0	33.0	37.0	27.0	37.0
80-81	34.7271943116004	37.0	33.0	37.0	30.0	37.0
82-83	34.58137843399322	37.0	33.0	37.0	27.0	37.0
84-85	34.413168231051635	37.0	33.0	37.0	27.0	37.0
86-87	34.27163526090543	37.0	33.0	37.0	27.0	37.0
88-89	34.13858074756956	37.0	33.0	37.0	27.0	37.0
90-91	34.06633707089021	37.0	33.0	37.0	27.0	37.0
92-93	34.023290062471304	37.0	33.0	37.0	27.0	37.0
94-95	34.03306725058993	37.0	33.0	37.0	27.0	37.0
96-97	33.99723124494955	37.0	33.0	37.0	27.0	37.0
98-99	34.03388715333243	37.0	33.0	37.0	27.0	37.0
100	34.04209879678908	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	4.0
19	11.0
20	67.0
21	383.0
22	1451.0
23	4152.0
24	9237.0
25	17673.0
26	30113.0
27	47555.0
28	69293.0
29	95574.0
30	127781.0
31	167644.0
32	218122.0
33	287540.0
34	381879.0
35	536300.0
36	877499.0
37	1788288.0
38	1649779.0
39	34099.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.799622220758124	20.539902144332224	14.915975327081357	23.744500307828297
2	14.501739001324939	20.60501421243084	42.233837280670365	22.65940950557385
3	18.049865977265785	25.924280859195587	31.05457907593508	24.97127408760355
4	12.173944293452777	18.324595116318402	38.00928163990875	31.49217895032007
5	13.118293773450269	37.697680536808654	35.67640409198533	13.507621597755742
6	29.860484250395423	37.76749581720702	18.320105225910225	14.051914706487329
7	26.172092279151286	32.92726156503839	22.26065479328767	18.639991362522647
8	24.297409103962742	37.24405566695658	20.768984399898745	17.689550829181933
9	24.76384633595749	16.96129459966654	20.800357985784597	37.47450107859137
10-11	24.05479995734811	26.751696716994378	28.448819582104456	20.744683743553058
12-13	24.940896407507193	24.271405083783744	28.58369366598948	22.204004842719584
14-15	22.17625941945641	25.891081739015853	26.04644755363466	25.886211287893072
16-17	21.657746003354745	29.303070748809272	26.796799909716306	22.242383338119673
18-19	21.545963193634243	27.72400458605842	29.02190356730911	21.70812865299823
20-21	22.11800707002738	26.73699494991289	29.080291167730387	22.064706812329344
22-23	22.07933098716438	27.077285642785924	29.023160864956036	21.820222505093657
24-25	22.04185605705259	27.308273915376258	29.028219003266692	21.62165102430446
26-27	21.671493825923072	27.346835710164147	29.22160883946217	21.76006162445061
28-29	21.34324955954316	26.897814881172323	29.67617456932517	22.082760989959343
30-31	21.774083164344262	26.871264880746697	29.12018924143487	22.23446271347417
32-33	21.97001512108578	27.05392638708587	29.430022412626517	21.54603607920183
34-35	21.911743809039876	27.486541873708664	29.27878335747105	21.322930959780408
36-37	21.739815977177734	27.15101931714582	28.9374853820709	22.17167932360555
38-39	21.730674599436586	27.09361951642925	29.204718403366265	21.9709874807679
40-41	21.792609034219765	26.978165978832923	29.329890190406694	21.899334796540618
42-43	21.87976987154673	27.011660212989508	29.265532116546428	21.843037798917333
44-45	21.733698086605898	27.301322405365603	28.93818482170892	22.02679468631958
46-47	22.266374279544806	27.04895982489539	28.246029509582588	22.438636385977215
48-49	21.995595411285713	27.17507617089778	28.452806309915392	22.376522107901117
50-51	21.925766927283572	27.55806305202676	28.3451395640954	22.17103045659427
52-53	22.015354175202013	27.71398034626794	28.421212484394005	21.84945299413604
54-55	22.008946843984045	27.715810941689732	28.07620311431998	22.19903910000624
56-57	22.244253659288123	27.907584814269242	27.75828723317342	22.08987429326921
58-59	21.909690738023283	28.133943580543697	28.12741426399798	21.82895141743504
60-61	22.16650226930515	28.414839375461533	27.604737061196737	21.813921294036582
62-63	21.957623952235192	28.55628922843026	27.64761876802137	21.838468051313175
64-65	22.02939949828125	28.2637592573036	27.675999671840167	22.03084157257498
66-67	22.18894096001404	28.023393275730168	27.607607966846615	22.180057797409173
68-69	22.138920518917306	27.892800137100636	27.78875566830824	22.179523675673813
70-71	22.163653475416307	27.686861589817095	27.851038407074657	22.29844652769194
72-73	22.23724004324841	27.730001253154747	27.729973931467004	22.30278477212984
74-75	22.06124691586114	27.892381439337306	27.7514997475602	22.294871897241354
76-77	22.228739713786926	27.764918067883666	27.713310637042753	22.293031581286655
78-79	22.16670325054183	27.697728071387345	27.659079453926662	22.476489224144164
80-81	22.17838235991893	27.81530997199019	27.64292234450939	22.36338532358149
82-83	22.24027129485688	27.752254716571436	27.7422577223217	22.265216266249986
84-85	22.26344631543816	27.731346060523528	27.720508831861835	22.28469879217648
86-87	22.201529466952643	27.680072808886745	27.81794524458727	22.300452479573345
88-89	22.249924262853657	27.6824237452961	27.717338692113696	22.350313299736545
90-91	22.26411520378396	27.67066766765993	27.74554402458338	22.319673103972725
92-93	22.261812525090324	27.64312525090325	27.7908972300281	22.304164993978322
94-95	22.12675089222042	27.634061133237026	27.878258493250158	22.360929481292388
96-97	22.286874709476706	27.711489271088418	27.880690036641642	22.12094598279323
98-99	23.298820326443614	29.641654285331537	28.10622977106539	18.953295617159466
100	22.338966431773745	30.620507156063013	22.008848355257328	25.03167805690591
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2042.0
1	1664.0
2	1856.0
3	2549.0
4	3305.5
5	5349.5
6	7356.0
7	9472.0
8	11368.0
9	11744.5
10	10544.5
11	8962.5
12	9342.0
13	12151.0
14	19095.5
15	29085.5
16	37163.5
17	41651.5
18	43183.0
19	41738.0
20	38104.0
21	33802.0
22	30711.0
23	30758.5
24	34481.0
25	41636.0
26	51042.0
27	61761.5
28	72943.0
29	85636.0
30	99056.0
31	111861.5
32	126616.5
33	141878.0
34	156229.0
35	169330.0
36	183281.5
37	195371.5
38	204291.0
39	212271.5
40	219484.0
41	225980.5
42	229887.5
43	234849.5
44	239700.5
45	244211.5
46	246752.5
47	247680.5
48	261409.5
49	270818.5
50	265102.5
51	261820.0
52	257151.0
53	240948.5
54	218838.0
55	199365.0
56	181164.0
57	163465.5
58	145461.5
59	125625.5
60	104737.0
61	84844.0
62	66918.5
63	50999.0
64	37047.0
65	26370.5
66	19140.0
67	14389.0
68	10798.0
69	7656.5
70	5230.0
71	3578.5
72	2365.5
73	1483.0
74	957.5
75	626.5
76	436.5
77	315.0
78	217.5
79	159.0
80	120.0
81	87.5
82	68.0
83	51.5
84	38.5
85	27.5
86	19.5
87	14.5
88	10.5
89	7.0
90	5.0
91	4.0
92	3.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0014658490276377165
5	0.006856390613144158
6	1.5761817501480824E-5
7	1.5761817501480824E-5
8	0.0
9	9.457090500888494E-4
10-11	9.693517763410707E-4
12-13	0.0012136599476140235
14-15	0.001016637228845513
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	119.0
20-21	451.0
22-23	946.0
24-25	1944.0
26-27	3017.0
28-29	5089.0
30-31	10312.0
32-33	12897.0
34-35	18637.0
36-37	25422.0
38-39	30242.0
40-41	32571.0
42-43	39769.0
44-45	47556.0
46-47	45789.0
48-49	42119.0
50-51	36417.0
52-53	36287.0
54-55	46722.0
56-57	52881.0
58-59	50247.0
60-61	52946.0
62-63	52483.0
64-65	56356.0
66-67	53695.0
68-69	44680.0
70-71	44072.0
72-73	42298.0
74-75	43858.0
76-77	42918.0
78-79	48235.0
80-81	49734.0
82-83	48061.0
84-85	49494.0
86-87	53466.0
88-89	59650.0
90-91	64235.0
92-93	69356.0
94-95	82206.0
96-97	217385.0
98-99	397459.0
100-101	4232425.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.42275086497511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.82990600046082	79.40600243367396
2	6.7864048893337285	11.865723678181432
3	1.4095218134569736	3.6967282300979085
4	0.48343506565980826	1.6905289321828123
5	0.2032406982137391	0.8883930462781653
6	0.09589718566895758	0.5030157462833725
7	0.06140337035606401	0.3757636084235588
8	0.036179260038833	0.2530312349483238
9	0.019793451612400478	0.15573581901619446
>10	0.07195292549098715	0.9649242939953971
>50	0.0018289734641744096	0.10617293244534506
>100	3.834638925654923E-4	0.06364090422578894
>500	5.290235104711251E-5	0.03033914024765387
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5761817501480824E-5	0.0
2	1.5761817501480824E-5	0.0	0.0	1.5761817501480824E-5	0.0
3	1.5761817501480824E-5	0.0	0.0	1.5761817501480824E-5	0.0
4	1.5761817501480824E-5	3.152363500296165E-5	0.0	1.5761817501480824E-5	0.0
5	1.5761817501480824E-5	3.152363500296165E-5	0.0	3.152363500296165E-5	0.0
6	3.152363500296165E-5	3.152363500296165E-5	0.0	3.152363500296165E-5	0.0
7	3.152363500296165E-5	3.152363500296165E-5	0.0	4.728545250444247E-5	0.0
8	3.152363500296165E-5	3.152363500296165E-5	0.0	4.728545250444247E-5	0.0
9	3.152363500296165E-5	1.1033272251036576E-4	0.0	7.880908750740411E-5	3.152363500296165E-5
10-11	3.940454375370206E-5	1.1033272251036576E-4	0.0	1.260945400118466E-4	3.152363500296165E-5
12-13	6.30472700059233E-5	1.1033272251036576E-4	0.0	1.4185635751332742E-4	3.152363500296165E-5
14-15	6.30472700059233E-5	1.5761817501480822E-4	0.0	1.7337999251628905E-4	3.152363500296165E-5
16-17	9.457090500888494E-5	1.5761817501480822E-4	0.0	1.970227187685103E-4	4.728545250444247E-5
18-19	9.457090500888494E-5	1.970227187685103E-4	0.0	2.2066544502073152E-4	4.728545250444247E-5
20-21	9.457090500888494E-5	2.3642726252221235E-4	0.0	2.2066544502073152E-4	4.728545250444247E-5
22-23	9.457090500888494E-5	2.8371271502665484E-4	0.0	2.443081712729528E-4	5.516636125518288E-5
24-25	1.0245181375962534E-4	3.07355441278876E-4	0.0	2.6795089752517395E-4	6.30472700059233E-5
26-27	1.1033272251036576E-4	3.1523635002961645E-4	0.0	3.8616452878628016E-4	6.30472700059233E-5
28-29	1.1033272251036576E-4	3.1523635002961645E-4	0.0	5.910681563055308E-4	6.30472700059233E-5
30-31	1.1033272251036576E-4	3.467599850325781E-4	0.0	0.001166374495109581	6.30472700059233E-5
32-33	1.1033272251036576E-4	3.467599850325781E-4	0.0	0.0024430817127295275	7.092817875666371E-5
34-35	1.3397544876258698E-4	4.01926346287761E-4	0.0	0.004334499812907226	7.880908750740411E-5
36-37	1.5761817501480822E-4	4.5709270754294386E-4	0.0	0.007408054225695987	7.880908750740411E-5
38-39	1.5761817501480822E-4	4.728545250444247E-4	0.0	0.012042028571131349	7.880908750740411E-5
40-41	1.7337999251628905E-4	4.728545250444247E-4	0.0	0.016203148391522287	7.880908750740411E-5
42-43	1.7337999251628905E-4	4.807354337951651E-4	0.0	0.019954460956874722	7.880908750740411E-5
44-45	1.7337999251628905E-4	5.516636125518288E-4	0.0	0.023516631712209385	7.880908750740411E-5
46-47	1.7337999251628905E-4	6.225917913084925E-4	0.0	0.027827488798864392	8.668999625814453E-5
48-49	1.7337999251628905E-4	6.304727000592329E-4	0.0	0.03191768044049867	9.457090500888494E-5
50-51	1.8914181001776988E-4	7.014008788158966E-4	0.0	0.03524342393331112	9.457090500888494E-5
52-53	1.8914181001776988E-4	7.09281787566637E-4	0.0	0.03893957013740837	1.0245181375962534E-4
54-55	1.8914181001776988E-4	7.09281787566637E-4	0.0	0.04202100545894787	1.1821363126110617E-4
56-57	2.2854635377147193E-4	7.09281787566637E-4	0.0	0.04537827258676329	1.3397544876258698E-4
58-59	2.3642726252221235E-4	7.09281787566637E-4	0.0	0.048940443342097954	1.418563575133274E-4
60-61	2.521890800236932E-4	7.09281787566637E-4	0.0	0.052486852279931134	1.5761817501480822E-4
62-63	2.521890800236932E-4	7.09281787566637E-4	0.0	0.05586776213399878	1.5761817501480822E-4
64-65	2.521890800236932E-4	8.196145100770027E-4	0.0	0.05912257744805457	1.6549908376554864E-4
66-67	2.67950897525174E-4	8.274954188277432E-4	0.0	0.06284236637840404	1.7337999251628905E-4
68-69	2.67950897525174E-4	8.590190538307048E-4	0.0	0.06703500983379794	1.8914181001776986E-4
70-71	2.67950897525174E-4	8.668999625814453E-4	0.0	0.0711803678366874	2.049036275192507E-4
72-73	2.758318062759144E-4	8.668999625814453E-4	0.0	0.0736392113669184	2.049036275192507E-4
74-75	2.837127150266548E-4	8.826617800829261E-4	0.0	0.07586950854337793	2.049036275192507E-4
76-77	2.837127150266548E-4	8.905426888336665E-4	0.0	0.07796583027107488	2.127845362699911E-4
78-79	2.837127150266548E-4	8.984235975844068E-4	0.0	0.0796050592912289	2.2066544502073152E-4
80-81	2.837127150266548E-4	8.984235975844068E-4	0.0	0.08012519926877776	2.2066544502073152E-4
82-83	2.837127150266548E-4	8.984235975844068E-4	0.0	0.08037738834880145	2.2066544502073152E-4
84-85	2.837127150266548E-4	8.984235975844068E-4	0.0	0.08053500652381626	2.2066544502073152E-4
86-87	2.994745325281356E-4	9.299472325873685E-4	0.0	0.08057441106756996	2.2854635377147193E-4
88	2.994745325281356E-4	0.0010087563200947727	0.0	0.08059017288507145	2.3642726252221235E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9880	0.0	54.71209	1
CTTATAC	6805	0.0	48.00254	1
GTATCAA	17210	0.0	35.93573	1
TTATACA	9630	0.0	35.12747	2
TATACAC	10320	0.0	34.572075	3
TCAACGC	21570	0.0	27.993559	4
ATCAACG	21745	0.0	27.788063	3
TATCAAC	22270	0.0	27.34556	2
CAACGCA	22100	0.0	27.302961	5
GTGGTAT	4365	0.0	27.11434	1
AACGCAG	22600	0.0	26.772978	6
TGGTATC	4290	0.0	25.983227	2
TACACAT	14285	0.0	25.367977	5
ACGCAGA	25560	0.0	23.622368	7
ACACATC	14635	0.0	23.289074	6
ACATCTC	14745	0.0	23.174253	8
CGCAGAG	26170	0.0	23.088379	8
ATACACA	17940	0.0	21.854784	4
GTACATG	19850	0.0	21.334633	1
CACATCT	15965	0.0	21.052744	7
>>END_MODULE