FastQCFastQC Report
Fri 27 May 2016
SRR2139546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8314540
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC133400.058.4820251
GGTATCA103050.049.6272131
TATACAC173250.046.7055743
TTATACA171650.046.1605952
GTATCAA185700.036.7570721
TACACAT255100.031.8186045
TCAACGC221900.030.0974774
ATCAACG224150.029.7953623
CAACGCA226850.029.4590975
ATACACA286100.029.2678134
TATCAAC233300.028.8609682
AACGCAG233950.028.6165816
ACACATC281150.028.194936
ACATCTC299000.026.5125168
CACATCT305600.025.7746377
ACGCAGA268450.024.8611777
CGCAGAG277900.024.031058
CATCTCC339750.023.1594229
GTACATG242850.021.33571
TACATGG240050.020.9188062
Produced by FastQC (version 0.11.5)