##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139546_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8314540
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54532950710442	33.0	33.0	33.0	33.0	33.0
2	32.56078736767157	33.0	33.0	33.0	33.0	33.0
3	32.564010155703144	33.0	33.0	33.0	33.0	33.0
4	36.454769957207496	37.0	37.0	37.0	37.0	37.0
5	36.4654688052496	37.0	37.0	37.0	37.0	37.0
6	36.455839529306495	37.0	37.0	37.0	37.0	37.0
7	36.432466979532244	37.0	37.0	37.0	37.0	37.0
8	36.39551388290874	37.0	37.0	37.0	37.0	37.0
9	36.44331520444907	37.0	37.0	37.0	37.0	37.0
10-11	36.40273923752848	37.0	37.0	37.0	37.0	37.0
12-13	36.384962788079676	37.0	37.0	37.0	37.0	37.0
14-15	38.31662960308087	40.0	37.0	40.0	37.0	40.0
16-17	38.25497712441097	40.0	37.0	40.0	37.0	40.0
18-19	38.262317638738885	40.0	37.0	40.0	37.0	40.0
20-21	38.25068546326935	40.0	37.0	40.0	37.0	40.0
22-23	38.26519054372535	40.0	37.0	40.0	37.0	40.0
24-25	38.237268017277884	40.0	37.0	40.0	37.0	40.0
26-27	38.08504227256293	40.0	37.0	40.0	33.0	40.0
28-29	38.0017597756475	40.0	37.0	40.0	33.0	40.0
30-31	37.924790608162205	40.0	37.0	40.0	33.0	40.0
32-33	37.85590011029478	40.0	37.0	40.0	33.0	40.0
34-35	37.800248228336244	40.0	37.0	40.0	33.0	40.0
36-37	37.74049468216833	40.0	37.0	40.0	33.0	40.0
38-39	37.66806075407143	40.0	37.0	40.0	33.0	40.0
40-41	37.66244283359988	40.0	37.0	40.0	33.0	40.0
42-43	37.62222019744365	40.0	37.0	40.0	33.0	40.0
44-45	37.53644705977577	40.0	37.0	40.0	33.0	40.0
46-47	37.49825708975073	40.0	37.0	40.0	33.0	40.0
48-49	37.520435430269	40.0	37.0	40.0	33.0	40.0
50-51	37.375324098197055	40.0	37.0	40.0	33.0	40.0
52-53	37.25485208775622	40.0	37.0	40.0	33.0	40.0
54-55	37.1440252036674	38.5	37.0	40.0	33.0	40.0
56-57	37.02307128238601	37.0	37.0	40.0	33.0	40.0
58-59	36.902709727295786	37.0	37.0	40.0	33.0	40.0
60-61	36.74007426756792	37.0	37.0	40.0	33.0	40.0
62-63	36.57343155895173	37.0	37.0	40.0	33.0	40.0
64-65	36.433737676588535	37.0	37.0	40.0	33.0	40.0
66-67	36.22651887561049	37.0	37.0	40.0	33.0	40.0
68-69	36.03847996390869	37.0	37.0	40.0	33.0	40.0
70-71	35.796067228265215	37.0	35.0	37.0	33.0	40.0
72-73	35.591174521899106	37.0	33.0	37.0	33.0	40.0
74-75	35.3931301582669	37.0	33.0	37.0	33.0	40.0
76-77	33.617468876964544	35.0	33.0	37.0	27.0	37.0
78-79	34.87757383717718	37.0	33.0	37.0	33.0	37.0
80-81	34.97692873653257	37.0	33.0	37.0	33.0	37.0
82-83	34.83873365957567	37.0	33.0	37.0	33.0	37.0
84-85	34.68629907285212	37.0	33.0	37.0	33.0	37.0
86-87	34.557724031945305	37.0	33.0	37.0	33.0	37.0
88-89	34.43645204643478	37.0	33.0	37.0	30.0	37.0
90-91	34.37393335025931	37.0	33.0	37.0	27.0	37.0
92-93	34.33208067348525	37.0	33.0	37.0	27.0	37.0
94-95	34.34413504026607	37.0	33.0	37.0	33.0	37.0
96-97	34.3020281426208	37.0	33.0	37.0	30.0	37.0
98-99	34.32504954722037	37.0	33.0	37.0	33.0	37.0
100	34.32903811536474	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	3.0
18	6.0
19	28.0
20	113.0
21	462.0
22	1761.0
23	4887.0
24	10451.0
25	20038.0
26	33593.0
27	52708.0
28	77072.0
29	106751.0
30	143327.0
31	190246.0
32	251293.0
33	334431.0
34	454710.0
35	648467.0
36	1096711.0
37	2383092.0
38	2443338.0
39	61049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.35583447791459	20.535627948148665	14.39724867521234	24.711288898724405
2	14.204093070693027	20.44838319377861	42.135596196542444	23.211927538985922
3	17.703180212014132	26.52507534992916	30.89041606631275	24.881328371743958
4	12.467584825494777	18.488397168620725	37.2525089925386	31.79150901334589
5	13.289284258463738	37.67534621800648	35.31150028223787	13.723869241291911
6	29.639818832988674	37.553920614711245	18.459774914733686	14.346485637566392
7	25.901291701199547	32.882652904749136	22.148251394334668	19.06780399971664
8	24.262003670678116	36.47339479995285	21.494634700175837	17.769966829193194
9	24.683208759287727	16.893472172476258	20.42226163412058	38.00105743411543
10-11	23.990606971741837	26.34313370392044	28.556427175797072	21.109832148540654
12-13	25.140466465570743	23.70367697644099	28.37210323244861	22.783753325539664
14-15	22.317596824618683	25.526768646356846	25.54397961336302	26.611654915661454
16-17	22.317951444096725	28.870821476594017	26.00828187728966	22.802945202019593
18-19	22.272464862758493	27.59929593218627	27.70078681442389	22.427452390631352
20-21	23.14217385842277	26.43705775870317	27.74321831779606	22.677550065078
22-23	22.4147631608528	27.41483202404065	27.734549671739106	22.435855143367444
24-25	22.699282653849306	27.76906375773261	27.681009880164797	21.850643708253287
26-27	22.72004194080447	27.967083545280946	27.575898407644328	21.736976106270255
28-29	22.04506056048139	27.45156790308637	28.10970140091143	22.393670135520814
30-31	23.319309972507575	27.20425683442888	27.754449054719444	21.721984138344105
32-33	21.7706812446215	28.147281499888805	28.071378153373104	22.01065910211659
34-35	22.252115752761124	27.92499053489396	28.390802256689796	21.432091455655115
36-37	22.029480526243503	27.725496059812528	27.961051573410334	22.28397184053364
38-39	22.19379691203491	26.731704283385948	28.459566120677465	22.614932683901678
40-41	22.20863750478369	26.59332566048621	28.69593361540114	22.502103219328955
42-43	22.509569747057476	26.447752050700057	28.806696798962278	22.235981403280185
44-45	22.094827080457442	27.02948113857983	28.383759738335435	22.491932042627298
46-47	22.885563518124606	26.429287609857756	27.526692005167202	23.158456866850432
48-49	22.503094699889864	26.534309305406133	27.950506490975535	23.012089503728472
50-51	22.210870469388798	27.028427725127173	28.045988042686936	22.714713762797096
52-53	22.294163607549784	27.304978584291117	28.299624543628205	22.10123326453089
54-55	22.267830179629396	27.350944273697962	27.65578346674053	22.725442079932108
56-57	22.791841051875743	27.718349626344114	27.042776027023475	22.447033294756668
58-59	22.09476696014579	28.155659569931245	27.837620421582354	21.91195304834061
60-61	22.664597189662523	28.54613093139483	26.791636246475687	21.99763563246696
62-63	22.313969408631788	28.756386313468667	26.83332088575202	22.096323392147525
64-65	22.353954884050204	28.313036569818305	26.989377885152173	22.343630660979315
66-67	22.541602445149607	27.898653132775557	26.984518133680407	22.57522628839443
68-69	22.556996763783644	27.648985849306012	27.212505348202647	22.581512038707697
70-71	22.5684508529336	27.414318711762192	27.25007230146026	22.767158133843942
72-73	22.65964455185417	27.430611073395383	27.188953748287865	22.720790626462577
74-75	22.50480835077683	27.630815944209026	27.165558042025456	22.69881766298869
76-77	22.627939329172232	27.58289962979687	27.086345918894715	22.702815122136176
78-79	22.577246123484464	27.53341839229575	27.077775333210425	22.811560151009363
80-81	22.564467995576322	27.58007782444999	27.10663087494479	22.748823305028903
82-83	22.667128560595902	27.521342206059842	27.213019598176746	22.59850963516751
84-85	22.77595512417489	27.488072800469602	27.087089007834514	22.64888306752099
86-87	22.55800071133224	27.553395791583558	27.213213445350593	22.67539005173361
88-89	22.6482160612779	27.509896552628426	27.129058186895318	22.712829199198357
90-91	22.663785336648143	27.550111029045105	27.159715871215106	22.62638776309165
92-93	22.682405401953773	27.508844104546164	27.186399754173763	22.6223507393263
94-95	22.49737066824118	27.583587182129964	27.2277919195392	22.69125023008965
96-97	22.64418501742096	27.670337013300944	27.248216665287845	22.43726130399025
98-99	23.74894375815607	29.566619542439266	27.425872248317507	19.258564451087157
100	22.669442892265362	30.60576743489664	21.2386414547799	25.486148218058098
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2005.0
1	1701.0
2	2100.5
3	3012.0
4	4141.0
5	6765.5
6	9020.0
7	10409.0
8	11090.5
9	10782.0
10	9889.0
11	8961.0
12	9730.0
13	12938.5
14	20370.5
15	30689.5
16	39239.0
17	44735.0
18	46279.0
19	43868.5
20	39879.0
21	35723.0
22	33231.5
23	34035.5
24	38461.5
25	47186.5
26	59696.5
27	74002.5
28	89009.0
29	106581.5
30	122794.5
31	138342.5
32	157284.0
33	175352.5
34	192851.5
35	210151.0
36	230390.0
37	250891.0
38	264326.0
39	276256.5
40	292525.5
41	305132.5
42	308723.5
43	311770.0
44	317693.5
45	328999.0
46	354075.5
47	384406.5
48	393102.0
49	397160.0
50	408537.5
51	399327.0
52	370470.5
53	336196.0
54	307406.5
55	283456.5
56	259768.0
57	233577.5
58	205917.5
59	179390.0
60	149411.5
61	120432.0
62	95249.5
63	72715.5
64	54097.0
65	39438.0
66	28815.0
67	21762.5
68	16589.5
69	12045.5
70	8723.0
71	6316.0
72	4351.0
73	2901.0
74	1903.0
75	1251.5
76	862.5
77	588.5
78	401.5
79	269.5
80	184.5
81	131.0
82	100.0
83	74.0
84	55.0
85	40.0
86	28.5
87	24.5
88	19.5
89	12.0
90	8.0
91	6.0
92	4.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.001767987164653727
5	0.007589114972085046
6	2.4054247138145947E-5
7	1.2027123569072973E-5
8	0.0
9	8.900071441114001E-4
10-11	9.080478294650094E-4
12-13	0.0012027123569072974
14-15	8.900071441114001E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	176.0
20-21	514.0
22-23	1297.0
24-25	2709.0
26-27	4640.0
28-29	8133.0
30-31	18302.0
32-33	22615.0
34-35	33667.0
36-37	55586.0
38-39	56891.0
40-41	54425.0
42-43	61170.0
44-45	70857.0
46-47	68940.0
48-49	65987.0
50-51	60491.0
52-53	62847.0
54-55	83482.0
56-57	94208.0
58-59	88971.0
60-61	98746.0
62-63	95135.0
64-65	95970.0
66-67	93984.0
68-69	67245.0
70-71	64902.0
72-73	62313.0
74-75	63779.0
76-77	62988.0
78-79	67286.0
80-81	69727.0
82-83	66946.0
84-85	68465.0
86-87	71936.0
88-89	77418.0
90-91	80762.0
92-93	85334.0
94-95	104295.0
96-97	272547.0
98-99	492488.0
100-101	5236366.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.74950457651839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.10113907769667	74.62176254960559
2	7.486365541228163	12.539588103131551
3	1.8690920645604552	4.696066032445202
4	0.6806070424918308	2.2800201047992092
5	0.3248277509401082	1.3602081606969383
6	0.16990387293741413	0.8537619110484097
7	0.10506626251364144	0.6159473203256728
8	0.06712767053691791	0.44975273206741007
9	0.04429067754271882	0.33383900714049275
>10	0.14783770284964257	1.948204759057588
>50	0.0030839479778293796	0.17443641890225187
>100	5.831763480547151E-4	0.07887646129077219
>500	5.0141609991771605E-5	0.02622484704366796
>1k	2.507076657864876E-5	0.021311592445200995
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2027123569072973E-5
2	0.0	1.2027123569072973E-5	0.0	0.0	1.2027123569072973E-5
3	0.0	1.2027123569072973E-5	0.0	0.0	1.2027123569072973E-5
4	0.0	6.013561784536487E-5	0.0	0.0	1.2027123569072973E-5
5	0.0	8.418986498351082E-5	0.0	1.2027123569072973E-5	1.2027123569072973E-5
6	2.4054247138145947E-5	8.418986498351082E-5	0.0	1.2027123569072973E-5	1.2027123569072973E-5
7	2.4054247138145947E-5	8.418986498351082E-5	0.0	2.4054247138145947E-5	2.4054247138145947E-5
8	2.4054247138145947E-5	1.2027123569072974E-4	0.0	2.4054247138145947E-5	2.4054247138145947E-5
9	2.4054247138145947E-5	3.127052127958973E-4	0.0	3.608137070721892E-5	2.4054247138145947E-5
10-11	2.4054247138145947E-5	3.127052127958973E-4	0.0	8.418986498351082E-5	2.4054247138145947E-5
12-13	2.4054247138145947E-5	3.127052127958973E-4	0.0	1.1425767390619325E-4	3.0067808922682434E-5
14-15	2.4054247138145947E-5	4.269628867020906E-4	0.0	1.2027123569072974E-4	6.614917962990136E-5
16-17	4.8108494276291894E-5	4.4500357205570003E-4	0.0	1.2027123569072974E-4	8.418986498351082E-5
18-19	4.8108494276291894E-5	4.6905781919384594E-4	0.0	1.6236616818248513E-4	9.621698855258379E-5
20-21	4.8108494276291894E-5	5.231798752546743E-4	0.0	1.804068535360946E-4	1.2027123569072974E-4
22-23	5.412205606082838E-5	5.953426166691122E-4	0.0	1.804068535360946E-4	1.2628479747526622E-4
24-25	6.614917962990136E-5	6.434511109454041E-4	0.0	1.9243397710516757E-4	1.4432548282887567E-4
26-27	7.216274141443784E-5	6.614917962990135E-4	0.0	2.46556033165996E-4	1.4432548282887567E-4
28-29	7.216274141443784E-5	6.675053580835501E-4	0.0	4.991256281165284E-4	1.6236616818248513E-4
30-31	7.216274141443784E-5	7.577087848515973E-4	0.0	0.0016657566143166067	1.6837972996702164E-4
32-33	7.216274141443784E-5	7.697359084206703E-4	0.0	0.003993005024932227	1.6837972996702164E-4
34-35	7.216274141443784E-5	8.719664587577905E-4	0.0	0.006825392625448913	1.9243397710516757E-4
36-37	7.216274141443784E-5	9.802105708794475E-4	0.0	0.011095021492469818	1.9243397710516757E-4
38-39	7.216274141443784E-5	0.0010102783798021297	0.0	0.018918665374151787	1.9243397710516757E-4
40-41	7.216274141443784E-5	0.0011546038626310054	0.0	0.026501766784452298	1.9844753888970405E-4
42-43	7.216274141443784E-5	0.001196698795122761	0.0	0.03345945776916101	2.2250178602785002E-4
44-45	7.216274141443784E-5	0.0012869022218908082	0.0	0.04095836931447801	2.4054247138145948E-4
46-47	7.216274141443784E-5	0.001359064963305246	0.0	0.047898019613833116	2.5858315673506893E-4
48-49	7.216274141443784E-5	0.001359064963305246	0.0	0.05444077483540881	2.8865096565775135E-4
50-51	7.216274141443784E-5	0.001521431131487731	0.0	0.05973872276758546	2.8865096565775135E-4
52-53	7.216274141443784E-5	0.0015274446932722677	0.0	0.06520505042972913	2.9466452744228785E-4
54-55	7.216274141443784E-5	0.0015454853786258773	0.0	0.07022637451981709	3.127052127958973E-4
56-57	7.216274141443784E-5	0.0015875803111176324	0.0	0.0746764102403741	3.4878658350311623E-4
58-59	7.216274141443784E-5	0.0015875803111176324	0.0	0.078946039107395	3.9088151599487165E-4
60-61	7.216274141443784E-5	0.0015935938729021689	0.0	0.08349229181650458	3.968950777794081E-4
62-63	9.621698855258379E-5	0.0016537294907475338	0.0	0.08801449027847602	4.089222013484811E-4
64-65	9.621698855258379E-5	0.0018642041532063109	0.0	0.09201350886519277	4.089222013484811E-4
66-67	1.0824411212165676E-4	0.0019002855239135298	0.0	0.09672814130426939	4.089222013484811E-4
68-69	1.0824411212165676E-4	0.001972448265327968	0.0	0.10140067881085424	4.1493576313301756E-4
70-71	1.0824411212165676E-4	0.002014543197819723	0.0	0.10586875521676485	4.32976448486627E-4
72-73	1.3229835925980272E-4	0.0020325838831733323	0.0	0.10967533982637645	5.051391899010649E-4
74-75	1.3229835925980272E-4	0.0021047466245877706	0.0	0.11279637839255088	5.051391899010649E-4
76-77	1.3229835925980272E-4	0.0022190042984939636	0.0	0.11566484736377478	5.051391899010649E-4
78-79	1.3229835925980272E-4	0.002285153478123865	0.0	0.11749898370805842	5.051391899010649E-4
80-81	1.3229835925980272E-4	0.0022911670399084014	0.0	0.11808229920115845	5.171663134701379E-4
82-83	1.3229835925980272E-4	0.002297180601692938	0.0	0.11864156044712035	5.412205606082838E-4
84-85	1.3229835925980272E-4	0.002297180601692938	0.0	0.11885203510957912	5.472341223928202E-4
86-87	1.3229835925980272E-4	0.002333261972400157	0.0	0.11892419785099356	5.532476841773568E-4
88	1.3229835925980272E-4	0.0024415060845218136	0.0	0.11892419785099356	5.532476841773568E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	13340	0.0	58.482025	1
GGTATCA	10305	0.0	49.627213	1
TATACAC	17325	0.0	46.705574	3
TTATACA	17165	0.0	46.160595	2
GTATCAA	18570	0.0	36.757072	1
TACACAT	25510	0.0	31.818604	5
TCAACGC	22190	0.0	30.097477	4
ATCAACG	22415	0.0	29.795362	3
CAACGCA	22685	0.0	29.459097	5
ATACACA	28610	0.0	29.267813	4
TATCAAC	23330	0.0	28.860968	2
AACGCAG	23395	0.0	28.616581	6
ACACATC	28115	0.0	28.19493	6
ACATCTC	29900	0.0	26.512516	8
CACATCT	30560	0.0	25.774637	7
ACGCAGA	26845	0.0	24.861177	7
CGCAGAG	27790	0.0	24.03105	8
CATCTCC	33975	0.0	23.159422	9
GTACATG	24285	0.0	21.3357	1
TACATGG	24005	0.0	20.918806	2
>>END_MODULE