FastQCFastQC Report
Fri 27 May 2016
SRR2139545_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139545_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9319618
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113900.049.4164921
GTATCAA219400.038.8352661
TCAACGC269200.031.0587144
ATCAACG270050.030.8676973
TATCAAC274950.030.5769482
CAACGCA274600.030.3562365
AACGCAG283250.029.5009216
ACGCAGA329300.025.3506437
CGCAGAG332450.025.073248
GCAGAGT388550.021.4748129
GTACATG287800.021.2634451
TACATGG283200.021.1493782
ACATGGG292700.020.4056953
GAGTACT226900.019.43186412-13
GTGGTAT59150.018.8752961
CATGGGG217050.018.5257134
AGAGTAC352350.018.29677410-11
CAGAGTA377900.018.2758910-11
TGGTATC58700.017.6614252
GTACTTT255200.017.21135114-15
Produced by FastQC (version 0.11.5)