FastQCFastQC Report
Fri 27 May 2016
SRR2139544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8878390
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112400.049.3942031
GTATCAA208650.037.5845531
TCAACGC252900.030.2299484
ATCAACG254350.030.0576133
TATCAAC261400.029.5742992
CAACGCA259950.029.475965
AACGCAG267250.028.6695256
ACGCAGA307400.024.883747
CGCAGAG316950.024.1612538
GTGGTAT56100.023.043391
GTACATG282200.022.1916521
TGGTATC53500.022.0829752
TACATGG283600.021.5538222
GCAGAGT367550.020.7189039
ACATGGG287700.020.6218743
GAGTACT212350.018.98852312-13
CATGGGG215350.018.74444
CAGAGTA357800.018.31637810-11
AGAGTAC332200.018.24602910-11
GTACTTT237650.016.79595814-15
Produced by FastQC (version 0.11.5)