##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139544_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8878390
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52184022103106	33.0	33.0	33.0	33.0	33.0
2	32.51943100044039	33.0	33.0	33.0	33.0	33.0
3	32.52033465526971	33.0	33.0	33.0	33.0	33.0
4	36.41041573979066	37.0	37.0	37.0	37.0	37.0
5	36.43297906489803	37.0	37.0	37.0	37.0	37.0
6	36.426466960789064	37.0	37.0	37.0	37.0	37.0
7	36.400848239376735	37.0	37.0	37.0	37.0	37.0
8	36.387417651173244	37.0	37.0	37.0	37.0	37.0
9	36.42835435253464	37.0	37.0	37.0	37.0	37.0
10-11	36.37026746966511	37.0	37.0	37.0	37.0	37.0
12-13	36.34954394884658	37.0	37.0	37.0	37.0	37.0
14-15	38.259042179944785	40.0	37.0	40.0	37.0	40.0
16-17	38.21339370088496	40.0	37.0	40.0	37.0	40.0
18-19	38.21550252917477	40.0	37.0	40.0	35.0	40.0
20-21	38.21072731332181	40.0	37.0	40.0	33.0	40.0
22-23	38.21969569294737	40.0	37.0	40.0	37.0	40.0
24-25	38.19949286402189	40.0	37.0	40.0	35.0	40.0
26-27	38.068717440957926	40.0	37.0	40.0	33.0	40.0
28-29	38.00056729825343	40.0	37.0	40.0	33.0	40.0
30-31	37.93784145024881	40.0	37.0	40.0	33.0	40.0
32-33	37.87408119507316	40.0	37.0	40.0	33.0	40.0
34-35	37.82794739699703	40.0	37.0	40.0	33.0	40.0
36-37	37.769853217115255	40.0	37.0	40.0	33.0	40.0
38-39	37.718768425640505	40.0	37.0	40.0	33.0	40.0
40-41	37.692179866624016	40.0	37.0	40.0	33.0	40.0
42-43	37.62605617873905	40.0	37.0	40.0	33.0	40.0
44-45	37.53798063880609	40.0	37.0	40.0	33.0	40.0
46-47	37.493302146795656	40.0	37.0	40.0	33.0	40.0
48-49	37.484719718415946	40.0	37.0	40.0	33.0	40.0
50-51	37.38869552106033	40.0	37.0	40.0	33.0	40.0
52-53	37.259772587896876	40.0	37.0	40.0	33.0	40.0
54-55	37.12478734426861	38.5	37.0	40.0	33.0	40.0
56-57	37.0109609194903	37.0	37.0	40.0	33.0	40.0
58-59	36.87110483790396	37.0	37.0	40.0	33.0	40.0
60-61	36.7074313462801	37.0	37.0	40.0	33.0	40.0
62-63	36.51419786160862	37.0	37.0	40.0	33.0	40.0
64-65	36.34757769610236	37.0	37.0	40.0	33.0	40.0
66-67	36.120112287448286	37.0	37.0	40.0	33.0	40.0
68-69	35.920991022307874	37.0	35.0	40.0	33.0	40.0
70-71	35.673215665388426	37.0	33.0	37.0	33.0	40.0
72-73	35.46051593892859	37.0	33.0	37.0	33.0	40.0
74-75	35.26051357493188	37.0	33.0	37.0	33.0	40.0
76-77	33.46189032954825	35.0	33.0	37.0	27.0	37.0
78-79	34.74368556319836	37.0	33.0	37.0	30.0	37.0
80-81	34.84735026459575	37.0	33.0	37.0	33.0	37.0
82-83	34.712554631509974	37.0	33.0	37.0	33.0	37.0
84-85	34.553731841338845	37.0	33.0	37.0	30.0	37.0
86-87	34.42169732880546	37.0	33.0	37.0	27.0	37.0
88-89	34.30018269948869	37.0	33.0	37.0	27.0	37.0
90-91	34.23699468747968	37.0	33.0	37.0	27.0	37.0
92-93	34.1945457685953	37.0	33.0	37.0	27.0	37.0
94-95	34.21155770150789	37.0	33.0	37.0	27.0	37.0
96-97	34.170676439316665	37.0	33.0	37.0	27.0	37.0
98-99	34.20095858674958	37.0	33.0	37.0	27.0	37.0
100	34.20795756605834	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	14.0
20	97.0
21	449.0
22	1576.0
23	4566.0
24	10469.0
25	20577.0
26	35867.0
27	56831.0
28	84144.0
29	117754.0
30	158404.0
31	210820.0
32	278457.0
33	368091.0
34	497814.0
35	708102.0
36	1182130.0
37	2530410.0
38	2549338.0
39	62477.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.47000751262335	20.34123303887304	14.343771787452456	23.84498766105116
2	14.744283591957553	21.015229112485486	41.70274115014096	22.537746145416005
3	18.37909801213959	26.262024984259536	30.02063437177236	25.338242631828518
4	12.721045266466266	18.0143838801064	38.649953504465735	30.6146173489616
5	13.70283490421251	37.54532833580676	35.112486588624904	13.639350171355838
6	30.024737595976024	37.54958247492873	18.803546454204696	13.622133474890546
7	26.611083747532067	32.46478217270772	23.13467525125904	17.789458828501168
8	24.357389121225808	36.7026904652758	21.037733192617132	17.902187220881263
9	25.61973669138341	17.123575196092688	21.35846089021051	35.898227222313395
10-11	24.468431755836278	26.745335962213506	28.534376775747173	20.251855506203043
12-13	25.26330230371987	24.275916552877902	28.742478987796417	21.718302155605812
14-15	22.37812789223346	25.569593648696156	26.506557615796623	25.545720843273767
16-17	21.898587469124468	28.575507496291557	27.173890761725943	22.352014272858028
18-19	21.87733924731849	27.772067908708674	28.967752036123667	21.38284080784917
20-21	22.740743748541714	26.943680881210764	28.82432074660612	21.491254623641403
22-23	22.46734952236129	26.853867826245835	28.679961703067885	21.998820948324994
24-25	22.22972755637042	26.99372385443993	28.756191126103754	22.020357463085897
26-27	22.14503758808722	27.16393531675334	28.673995472118634	22.017031623040804
28-29	22.0877650817763	27.217135226999034	28.727212822249864	21.967886868974798
30-31	22.409088823459992	27.105611542104157	28.51958567251292	21.965713961922926
32-33	22.16857880779819	27.2407514215021	28.516949193278084	22.073720577421625
34-35	22.309829992053956	27.23425751819752	28.498276844975013	21.957635644773514
36-37	22.31310467290028	27.197239126905583	28.423839246253284	22.065816953940857
38-39	22.1871362794545	27.28720174915269	28.51887046241463	22.00679150897818
40-41	22.438597840437705	27.142598053953915	28.345484641241864	22.07331946436652
42-43	22.282078017224112	27.285698308732254	28.132617286677768	22.299606387365866
44-45	22.39145430302114	27.40256427847698	27.971708415998926	22.234273002502952
46-47	22.480871713936452	27.36190171672428	27.66675606314393	22.49047050619534
48-49	22.452524088675187	27.476726703203212	27.624741025378093	22.44600818274351
50-51	22.441588755009803	27.58245757898412	27.505926675393866	22.47002699061221
52-53	22.58141257655164	27.52076465746105	27.363682852992856	22.534139912994455
54-55	22.61639270735819	27.399098404551918	27.37262191420174	22.611886973888154
56-57	22.557207061980417	27.581293216792258	27.35086883947216	22.510630881755166
58-59	22.504494762018588	27.590216899713916	27.402598194482536	22.50269014378496
60-61	22.636127058543078	27.55803775402769	27.35012862790282	22.455706559526412
62-63	22.612836899841014	27.66363697625121	27.240921925389188	22.482604198518587
64-65	22.581569621383604	27.54894088987312	27.243153945263348	22.62633554347993
66-67	22.664077770692888	27.587333255642704	27.097856858241915	22.65073211542249
68-69	22.54788920121186	27.564928325536815	27.246026418697888	22.641156054553438
70-71	22.55623191499946	27.341638355954267	27.308791279402378	22.793338449643898
72-73	22.666909839205427	27.429175998086	27.185009851102578	22.718904311605996
74-75	22.495143165303894	27.6130628238577	27.18874863530366	22.703045375534746
76-77	22.61502464090758	27.51893415755584	27.14172930107828	22.724311900458297
78-79	22.556496311110365	27.50807186192293	27.128205300573082	22.807226526393627
80-81	22.570276537127775	27.60735025591406	27.10424185494636	22.71813135201181
82-83	22.654885245267124	27.53137034718734	27.195272351923528	22.618472055622004
84-85	22.71963298683137	27.520426085002164	27.109203351999238	22.650737576167224
86-87	22.537519723424797	27.596909419602035	27.153551225943872	22.7120196310293
88-89	22.620671587855288	27.504476252405674	27.154560153548914	22.720292006190125
90-91	22.645731618032787	27.547074882975714	27.156808413813298	22.650385085178197
92-93	22.686730777362506	27.527187050971797	27.190551676921455	22.59553049474425
94-95	22.512133695051624	27.574899814899155	27.220569405725342	22.692397084323872
96-97	22.564710050245687	27.70043885701565	27.263927057299203	22.470924035439456
98-99	23.708389957126286	29.569211506091214	27.457536761619938	19.26486177516256
100	22.7155353009458	30.57383354740334	21.184825985122707	25.525805166528148
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2214.0
1	1882.5
2	2449.5
3	3555.0
4	4756.5
5	7670.5
6	10155.5
7	11797.5
8	12786.0
9	12473.0
10	11426.0
11	10421.0
12	11249.0
13	14665.5
14	22883.5
15	34672.5
16	44859.0
17	51128.5
18	52447.5
19	49557.0
20	44629.5
21	39350.0
22	36132.0
23	37014.0
24	41876.0
25	50943.0
26	63787.0
27	78410.0
28	94583.5
29	113781.5
30	133887.0
31	153629.0
32	174885.5
33	194710.0
34	215530.5
35	236499.0
36	257894.0
37	277956.5
38	291578.5
39	303498.0
40	316452.5
41	326949.5
42	333750.0
43	342388.5
44	351382.5
45	359199.0
46	363783.5
47	366761.5
48	370002.0
49	368430.5
50	363369.5
51	355593.5
52	344152.5
53	328681.0
54	310240.5
55	289899.0
56	267569.5
57	242827.0
58	216877.0
59	189652.0
60	158750.5
61	128565.5
62	102074.0
63	78380.0
64	58192.5
65	42708.0
66	31684.0
67	23908.0
68	18063.5
69	13229.0
70	9498.5
71	6696.5
72	4517.0
73	2938.5
74	1871.5
75	1211.5
76	850.5
77	582.5
78	391.5
79	282.0
80	219.0
81	174.5
82	127.0
83	81.5
84	56.0
85	40.5
86	29.0
87	21.5
88	13.5
89	8.0
90	4.0
91	3.0
92	3.0
93	2.0
94	0.5
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0013290697975646487
5	0.007490096740512637
6	1.1263303369191937E-5
7	3.378991010757581E-5
8	0.0
9	7.09588112259092E-4
10-11	9.573807863813146E-4
12-13	0.0010925404268116179
14-15	9.799073931196986E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	145.0
20-21	527.0
22-23	1162.0
24-25	2685.0
26-27	3862.0
28-29	6816.0
30-31	16813.0
32-33	21142.0
34-35	28734.0
36-37	41141.0
38-39	50119.0
40-41	52985.0
42-43	63371.0
44-45	74596.0
46-47	72009.0
48-49	65461.0
50-51	58752.0
52-53	57729.0
54-55	62100.0
56-57	64265.0
58-59	63639.0
60-61	63611.0
62-63	62633.0
64-65	66256.0
66-67	70185.0
68-69	70633.0
70-71	70965.0
72-73	68964.0
74-75	71682.0
76-77	69070.0
78-79	74599.0
80-81	76955.0
82-83	75807.0
84-85	76671.0
86-87	80657.0
88-89	86534.0
90-91	91351.0
92-93	96401.0
94-95	119181.0
96-97	306313.0
98-99	548229.0
100-101	5823640.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.16380054353087
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.6704229908562	73.74169371722078
2	7.700163530431035	12.807497279946741
3	2.022899209582642	5.0469595915619125
4	0.7248663591608356	2.411305652558686
5	0.3352389388455654	1.393987212228877
6	0.18060696539577142	0.901197698816779
7	0.10020490418353734	0.5833394465502324
8	0.0676520683314098	0.45009624936565484
9	0.04383089123007587	0.3280629146312904
>10	0.14953894320135347	1.9421695484165176
>50	0.003555159038917861	0.1983666017463535
>100	9.240546595691842E-4	0.12821174028475993
>500	7.198916802478506E-5	0.03871507974870389
>1k	2.3995915029938403E-5	0.028397266922754513
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1263303369191937E-5
2	0.0	5.631651684595968E-5	0.0	0.0	1.1263303369191937E-5
3	0.0	5.631651684595968E-5	0.0	0.0	1.1263303369191937E-5
4	0.0	1.238963370611113E-4	0.0	1.1263303369191937E-5	1.1263303369191937E-5
5	0.0	1.3515964043030323E-4	0.0	3.378991010757581E-5	1.1263303369191937E-5
6	0.0	1.3515964043030323E-4	0.0	3.378991010757581E-5	1.1263303369191937E-5
7	0.0	1.4642294379949518E-4	0.0	4.505321347676775E-5	1.1263303369191937E-5
8	0.0	1.576862471686871E-4	0.0	6.757982021515161E-5	1.1263303369191937E-5
9	0.0	2.8158258422979844E-4	0.0	7.884312358434355E-5	2.2526606738383874E-5
10-11	0.0	2.8158258422979844E-4	0.0	9.01064269535355E-5	2.2526606738383874E-5
12-13	0.0	2.8158258422979844E-4	0.0	1.070013820073234E-4	2.815825842297984E-5
14-15	0.0	3.7732066286792987E-4	0.0	1.1263303369191936E-4	3.942156179217178E-5
16-17	0.0	3.8295231455252583E-4	0.0	1.407912921148992E-4	5.631651684595968E-5
18-19	0.0	4.2237387634469763E-4	0.0	1.633178988532831E-4	6.194816853055565E-5
20-21	0.0	4.6179543813686943E-4	0.0	2.0273946064545484E-4	6.757982021515161E-5
22-23	0.0	5.631651684595968E-4	0.0	2.477926741222226E-4	1.0700138200732339E-4
24-25	0.0	6.532715954131324E-4	0.0	3.322674493911621E-4	1.3515964043030323E-4
26-27	0.0	6.870615055207082E-4	0.0	5.406385617212129E-4	1.3515964043030323E-4
28-29	0.0	6.870615055207082E-4	0.0	0.0016669688986404065	1.407912921148992E-4
30-31	0.0	7.20851415628284E-4	0.0	0.005575335167750009	1.4642294379949518E-4
32-33	0.0	7.20851415628284E-4	0.0	0.014039707649697749	1.576862471686871E-4
34-35	0.0	8.109578425818194E-4	0.0	0.02422173389544726	1.576862471686871E-4
36-37	0.0	8.89800966166163E-4	0.0	0.0392469805899493	1.576862471686871E-4
38-39	0.0	9.066959212199509E-4	0.0	0.06264649333944555	1.80212853907071E-4
40-41	5.6316516845959684E-6	9.911706964888904E-4	0.0	0.0808874131458519	2.0273946064545487E-4
42-43	1.1263303369191937E-5	0.0010136973032272743	0.0	0.09787247462659333	2.0837111233005083E-4
44-45	1.1263303369191937E-5	0.0011657518987113653	0.0	0.11505464391629563	2.1963441569924276E-4
46-47	1.1263303369191937E-5	0.0012840165840878807	0.0	0.12977014976814488	2.4216102243762664E-4
48-49	1.1263303369191937E-5	0.0012896482357724766	0.0	0.14171488299117296	2.5905597749141453E-4
50-51	1.1263303369191937E-5	0.00142480787620278	0.0	0.14967803847319164	2.759509325452025E-4
52-53	1.1263303369191937E-5	0.001441702831256568	0.0	0.15693723749463584	3.041091909681823E-4
54-55	1.1263303369191937E-5	0.0014529661346257597	0.0	0.16353753326898232	3.2663579770656615E-4
56-57	1.1263303369191937E-5	0.0014867560447333356	0.0	0.16856096657164193	3.322674493911621E-4
58-59	1.1263303369191937E-5	0.0014980193481025277	0.0	0.17353934666082477	3.378991010757581E-4
60-61	1.1263303369191937E-5	0.0015374409098946992	0.0	0.1784050937163157	3.60425707814142E-4
62-63	1.1263303369191937E-5	0.0015599675166330833	0.0	0.1835411600526672	3.7732066286792987E-4
64-65	1.1263303369191937E-5	0.0017232854154863664	0.0	0.18853080344521922	3.8295231455252583E-4
66-67	1.1263303369191937E-5	0.0017232854154863664	0.0	0.1935823950063018	3.8295231455252583E-4
68-69	1.1263303369191937E-5	0.001785233584016922	0.0	0.19870719803928416	3.998472696063138E-4
70-71	1.1263303369191937E-5	0.0018246551458090939	0.0	0.20348283866782152	4.3363717971388956E-4
72-73	1.1263303369191937E-5	0.0018302867974936898	0.0	0.2070364108808016	4.6179543813686943E-4
74-75	1.1263303369191937E-5	0.0019147615727626295	0.0	0.21030276885786725	4.674270898214654E-4
76-77	1.1263303369191937E-5	0.0019992363480315686	0.0	0.21332696581249527	4.786903931906573E-4
78-79	1.1263303369191937E-5	0.0020161313030853567	0.0	0.21529804390210389	4.843220448752533E-4
80-81	1.1263303369191937E-5	0.0020161313030853567	0.0	0.21601889531773216	4.955853482444452E-4
82-83	1.1263303369191937E-5	0.002033026258139145	0.0	0.21654827057608417	5.35006910036617E-4
84-85	1.1263303369191937E-5	0.0020668161682467203	0.0	0.21674537838504504	5.631651684595969E-4
86-87	1.1263303369191937E-5	0.002106237730038892	0.0	0.21685237976705235	5.631651684595969E-4
88	1.1263303369191937E-5	0.0022301340671000034	0.0	0.21686364307042155	5.631651684595969E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11240	0.0	49.394203	1
GTATCAA	20865	0.0	37.584553	1
TCAACGC	25290	0.0	30.229948	4
ATCAACG	25435	0.0	30.057613	3
TATCAAC	26140	0.0	29.574299	2
CAACGCA	25995	0.0	29.47596	5
AACGCAG	26725	0.0	28.669525	6
ACGCAGA	30740	0.0	24.88374	7
CGCAGAG	31695	0.0	24.161253	8
GTGGTAT	5610	0.0	23.04339	1
GTACATG	28220	0.0	22.191652	1
TGGTATC	5350	0.0	22.082975	2
TACATGG	28360	0.0	21.553822	2
GCAGAGT	36755	0.0	20.718903	9
ACATGGG	28770	0.0	20.621874	3
GAGTACT	21235	0.0	18.988523	12-13
CATGGGG	21535	0.0	18.7444	4
CAGAGTA	35780	0.0	18.316378	10-11
AGAGTAC	33220	0.0	18.246029	10-11
GTACTTT	23765	0.0	16.795958	14-15
>>END_MODULE