FastQCFastQC Report
Fri 27 May 2016
SRR2139543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12916992
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144850.049.9452861
GTATCAA263100.037.110091
TCAACGC321700.029.7491344
ATCAACG323050.029.5848733
CAACGCA329450.029.00975
TATCAAC334300.028.781712
AACGCAG339700.028.2084856
ACGCAGA393450.024.3336627
CGCAGAG405000.023.6293568
GTACATG377450.021.9132541
TACATGG380950.021.2490062
ACATGGG388150.020.4452383
GCAGAGT473600.020.0616959
GTGGTAT79900.019.487171
TGGTATC76550.018.8229032
GAGTACT273100.018.59930412-13
CAGAGTA464600.017.88095710-11
CATGGGG287600.017.7823184
AGAGTAC429200.017.76240310-11
GTACTTT298500.016.9515814-15
Produced by FastQC (version 0.11.5)