##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139543_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12916992
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50742022600927	33.0	33.0	33.0	33.0	33.0
2	32.506536506332125	33.0	33.0	33.0	33.0	33.0
3	32.50951397972531	33.0	33.0	33.0	33.0	33.0
4	36.39491338231068	37.0	37.0	37.0	37.0	37.0
5	36.41375422389361	37.0	37.0	37.0	37.0	37.0
6	36.40901898832174	37.0	37.0	37.0	37.0	37.0
7	36.38117488963375	37.0	37.0	37.0	37.0	37.0
8	36.366009361931944	37.0	37.0	37.0	37.0	37.0
9	36.41408797032622	37.0	37.0	37.0	37.0	37.0
10-11	36.350557970462475	37.0	37.0	37.0	37.0	37.0
12-13	36.33131955179658	37.0	37.0	37.0	35.0	37.0
14-15	38.226921561924016	40.0	37.0	40.0	37.0	40.0
16-17	38.173438986414176	40.0	37.0	40.0	33.0	40.0
18-19	38.16944463540737	40.0	37.0	40.0	33.0	40.0
20-21	38.16475650256879	40.0	37.0	40.0	33.0	40.0
22-23	38.17108521365107	40.0	37.0	40.0	33.0	40.0
24-25	38.149993691354425	40.0	37.0	40.0	33.0	40.0
26-27	38.01160009825909	40.0	37.0	40.0	33.0	40.0
28-29	37.940704190577925	40.0	37.0	40.0	33.0	40.0
30-31	37.88264494699573	40.0	37.0	40.0	33.0	40.0
32-33	37.817880686637615	40.0	37.0	40.0	33.0	40.0
34-35	37.76341137991311	40.0	37.0	40.0	33.0	40.0
36-37	37.701834670606445	40.0	37.0	40.0	33.0	40.0
38-39	37.637757932326814	40.0	37.0	40.0	33.0	40.0
40-41	37.613995105552505	40.0	37.0	40.0	33.0	40.0
42-43	37.552149724211034	40.0	37.0	40.0	33.0	40.0
44-45	37.46495222870048	40.0	37.0	40.0	33.0	40.0
46-47	37.415394479111484	40.0	37.0	40.0	33.0	40.0
48-49	37.41419485088444	40.0	37.0	40.0	33.0	40.0
50-51	37.30440427699331	40.0	37.0	40.0	33.0	40.0
52-53	37.17433944821009	40.0	37.0	40.0	33.0	40.0
54-55	37.03420076811608	37.0	37.0	40.0	33.0	40.0
56-57	36.90944071593299	37.0	37.0	40.0	33.0	40.0
58-59	36.76794590645188	37.0	37.0	40.0	33.0	40.0
60-61	36.597574941845195	37.0	37.0	40.0	33.0	40.0
62-63	36.407383229517805	37.0	37.0	40.0	33.0	40.0
64-65	36.24355344675331	37.0	37.0	40.0	33.0	40.0
66-67	36.01403937137712	37.0	37.0	40.0	33.0	40.0
68-69	35.81683669665978	37.0	33.0	38.5	33.0	40.0
70-71	35.562976668517564	37.0	33.0	37.0	33.0	40.0
72-73	35.35383903176023	37.0	33.0	37.0	33.0	40.0
74-75	35.15468722123211	37.0	33.0	37.0	33.0	40.0
76-77	33.353157555097404	35.0	33.0	37.0	27.0	37.0
78-79	34.634433992020604	37.0	33.0	37.0	27.0	37.0
80-81	34.74064603087895	37.0	33.0	37.0	33.0	37.0
82-83	34.60401883226472	37.0	33.0	37.0	30.0	37.0
84-85	34.44816351993362	37.0	33.0	37.0	27.0	37.0
86-87	34.316578029580526	37.0	33.0	37.0	27.0	37.0
88-89	34.19570302699697	37.0	33.0	37.0	27.0	37.0
90-91	34.13547270648512	37.0	33.0	37.0	27.0	37.0
92-93	34.100090029610925	37.0	33.0	37.0	27.0	37.0
94-95	34.1205668889759	37.0	33.0	37.0	27.0	37.0
96-97	34.08696157072566	37.0	33.0	37.0	27.0	37.0
98-99	34.12256377020326	37.0	33.0	37.0	27.0	37.0
100	34.13352518410993	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	28.0
20	127.0
21	683.0
22	2652.0
23	7490.0
24	17182.0
25	33040.0
26	57029.0
27	90446.0
28	132221.0
29	182942.0
30	244154.0
31	321718.0
32	419289.0
33	553372.0
34	742454.0
35	1049476.0
36	1738324.0
37	3641186.0
38	3596890.0
39	86288.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.44415356144836	20.207576191113226	14.247047609846009	24.101222637592407
2	14.792174524842935	20.6870608884793	41.670506569950646	22.850258016727114
3	18.39679083179737	25.785074419803	30.137589308718315	25.680545439681314
4	12.725418902613924	17.79070411096879	38.132608075461505	31.35126891095578
5	13.71737304591326	37.45299631279262	35.27687502206564	13.55275561922848
6	30.654710730915806	37.190933148728874	18.4698413027744	13.684514817580911
7	26.967261418138218	32.28650292575856	22.664316893592567	18.08191876251065
8	24.51288194650891	36.8348373986761	20.854940531046235	17.797340123768755
9	25.48080436662183	16.955397024176705	20.999278774095572	36.5645198351059
10-11	24.532795349835723	26.55843591295326	28.50758352812371	20.401185209087306
12-13	25.266163233363955	24.00863802702186	28.64705245017966	22.078146289434525
14-15	22.376089632330835	25.443325725445437	26.114909377722462	26.065675264501266
16-17	22.106690938571457	28.611641936450837	26.838458210704164	22.44320891427354
18-19	22.1450899714113	27.6984223571556	28.418806793408248	21.737680878024854
20-21	22.858711269067882	26.890588353882837	28.361442093105826	21.889258283943455
22-23	22.652769835190078	26.97481635782324	28.1640830480583	22.208330758928383
24-25	22.474205687739264	27.114061060704092	28.23403776673741	22.177695484819235
26-27	22.39402121781483	27.28179781781066	28.178255704109635	22.145925260264875
28-29	22.269131101462726	27.1801594312869	28.239474691855555	22.311234775394823
30-31	22.68830923978163	27.126952995689408	28.10689063852466	22.0778471260043
32-33	22.466499025824586	27.251687053023797	28.134089675108072	22.14772424604355
34-35	22.523832441427903	27.248714738175035	28.16354817056283	22.063904649834235
36-37	22.526119389871358	27.148010591849463	28.059919471641496	22.26595054663768
38-39	22.324033410755025	27.191832337496102	28.185608317233168	22.298525934515702
40-41	22.56597669460685	27.020766922627963	28.139423430159756	22.27383295260543
42-43	22.497505194162386	27.084577700004065	27.970363472953487	22.447553632880062
44-45	22.46587594840179	27.252391861042597	27.816780875489872	22.46495131506574
46-47	22.64612137938754	27.17590451551454	27.512228380168057	22.665745724929867
48-49	22.500200849596826	27.29013723960967	27.509598023033476	22.70006388776003
50-51	22.463418601198036	27.42095643543465	27.47826216498189	22.63736279838543
52-53	22.6057399187514	27.364830993273582	27.444678657162402	22.584750430812615
54-55	22.596563528927952	27.30341050485186	27.356553933289224	22.743472032930967
56-57	22.611952510859403	27.45013945128352	27.268100925934952	22.669807111922125
58-59	22.51159908325036	27.50436599696649	27.403208540145457	22.580826379637696
60-61	22.65259798032268	27.600067097010587	27.216644171077824	22.530690751588907
62-63	22.574217922616096	27.69730365429232	27.14287983985002	22.585598583241566
64-65	22.57845491188761	27.620988247882934	27.084776967005592	22.715779873223866
66-67	22.64725646783419	27.599937307399518	26.971852250632356	22.78095397413394
68-69	22.581820974688714	27.515677238495357	27.07911299178154	22.82338879503439
70-71	22.622415356489817	27.330844771613755	27.124605748034536	22.922134123861895
72-73	22.700213163834494	27.34901784907858	27.013161342909914	22.937607644177014
74-75	22.621692760616924	27.512743820828355	26.989081819279708	22.876481599275017
76-77	22.742849212772246	27.438496733196992	26.933445563903057	22.88520849012771
78-79	22.67517573695652	27.412771181498062	26.903521399103763	23.008531682441657
80-81	22.70740257227836	27.50305210446805	26.906925144701194	22.882620178552397
82-83	22.76914424506117	27.46031335795526	26.98792322983863	22.782619167144937
84-85	22.81754009755398	27.45262071875697	26.943749633380737	22.786089550308304
86-87	22.66472151331117	27.493350137087713	27.05152354114217	22.790404808458945
88-89	22.745020224094766	27.44047240256969	27.027085231444044	22.787422141891504
90-91	22.754947374859157	27.509103871825662	26.99490541103354	22.741043342281635
92-93	22.78630716021717	27.442316001785834	27.07799732564295	22.69337951235405
94-95	22.636562890965408	27.50160218380427	27.13615174782329	22.72568317740704
96-97	22.71689507179582	27.596446164016502	27.175048544292913	22.511610219894767
98-99	23.862524363950268	29.463304912153383	27.351172631529824	19.322998092366525
100	22.7719224594899	30.436370839233817	21.17386895452758	25.617837746748695
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2766.0
1	2384.0
2	2967.0
3	4111.5
4	5453.5
5	8892.5
6	11887.5
7	13936.5
8	15306.0
9	15176.5
10	14045.0
11	12631.0
12	13298.0
13	17377.0
14	27204.5
15	41124.0
16	53098.0
17	60833.5
18	63463.0
19	61091.5
20	55959.5
21	50647.0
22	47445.0
23	48866.5
24	55592.5
25	68084.0
26	86074.0
27	107043.0
28	131041.0
29	158444.0
30	185855.0
31	213675.0
32	243944.0
33	273425.5
34	302724.0
35	330173.5
36	360239.0
37	390345.0
38	414317.0
39	435696.5
40	452789.5
41	467494.5
42	479164.0
43	489419.5
44	500560.0
45	512881.0
46	520828.5
47	525078.0
48	537691.5
49	542217.0
50	533564.5
51	525189.5
52	513221.5
53	493241.0
54	467342.0
55	437468.5
56	404715.5
57	368694.5
58	329105.0
59	286379.0
60	240005.0
61	195551.5
62	155491.0
63	119363.0
64	88748.5
65	65376.5
66	48252.0
67	36171.5
68	27372.5
69	19573.5
70	13620.5
71	9682.5
72	6697.5
73	4508.0
74	2947.0
75	1957.0
76	1299.5
77	833.0
78	547.5
79	393.5
80	286.0
81	199.0
82	135.0
83	82.5
84	55.0
85	44.0
86	40.5
87	34.5
88	24.5
89	16.0
90	10.5
91	8.0
92	6.5
93	3.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0016025402818241274
5	0.0076178726440335335
6	3.870870245951999E-5
7	0.0
8	0.0
9	7.432070872227838E-4
10-11	8.903001565689597E-4
12-13	0.0011883571655072636
14-15	0.0010451349664070398
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	236.0
20-21	786.0
22-23	1726.0
24-25	4001.0
26-27	5662.0
28-29	8740.0
30-31	19779.0
32-33	25181.0
34-35	35272.0
36-37	50531.0
38-39	57632.0
40-41	64246.0
42-43	75905.0
44-45	90612.0
46-47	89202.0
48-49	83773.0
50-51	73131.0
52-53	71987.0
54-55	82468.0
56-57	90913.0
58-59	94026.0
60-61	97150.0
62-63	98024.0
64-65	106081.0
66-67	111934.0
68-69	107321.0
70-71	106854.0
72-73	104380.0
74-75	108706.0
76-77	105983.0
78-79	116671.0
80-81	119515.0
82-83	117824.0
84-85	120324.0
86-87	127962.0
88-89	137288.0
90-91	143476.0
92-93	151716.0
94-95	186163.0
96-97	462631.0
98-99	804955.0
100-101	8456225.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.27163627599475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.50504142374682	73.69959618030094
2	7.912305436940825	13.177412408990241
3	1.960477677693713	4.897565523123531
4	0.7646246094530275	2.5468616946418816
5	0.3302769318508314	1.375135026971697
6	0.18022644369449312	0.9004650519986328
7	0.10103175024784783	0.5889155411276148
8	0.06257266241212361	0.4168422388162373
9	0.04452082543093583	0.3336589783792751
>10	0.13504966549080655	1.7526509076903483
>50	0.0029597722426504976	0.16649402076224248
>100	8.798200588343397E-4	0.11987751473710062
>500	1.6490673746358342E-5	0.010051863426638843
>1k	1.6490063347837155E-5	0.01447304903361745
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.096696196761599E-5	0.0	1.5483480983807996E-5	0.0
3	0.0	3.096696196761599E-5	0.0	2.3225221475711992E-5	0.0
4	0.0	1.0838436688665597E-4	0.0	6.193392393523198E-5	7.741740491903998E-6
5	0.0	1.1612610737855996E-4	0.0	6.193392393523198E-5	7.741740491903998E-6
6	0.0	1.1612610737855996E-4	0.0	6.193392393523198E-5	1.5483480983807996E-5
7	0.0	1.1612610737855996E-4	0.0	6.193392393523198E-5	1.5483480983807996E-5
8	0.0	1.2386784787046397E-4	0.0	6.193392393523198E-5	1.5483480983807996E-5
9	0.0	2.1676873377331194E-4	0.0	7.741740491903997E-5	1.5483480983807996E-5
10-11	0.0	2.1676873377331194E-4	0.0	1.3160958836236795E-4	1.5483480983807996E-5
12-13	0.0	2.1676873377331194E-4	0.0	1.3935132885427195E-4	1.5483480983807996E-5
14-15	0.0	2.9418613869235187E-4	0.0	1.7418916106783994E-4	2.3225221475711992E-5
16-17	0.0	3.096696196761599E-4	0.0	2.0902699328140793E-4	3.8708702459519985E-5
18-19	0.0	3.4450745188972787E-4	0.0	2.283813445111679E-4	4.6450442951423985E-5
20-21	0.0	3.9482876508710387E-4	0.0	2.554774362328319E-4	5.4192183443327984E-5
22-23	0.0	4.2966659730067186E-4	0.0	3.174113601680639E-4	7.741740491903998E-5
24-25	3.870870245951999E-6	4.7611704025209584E-4	0.0	4.025705055790079E-4	1.0064262639475196E-4
26-27	7.741740491903998E-6	5.148257427116158E-4	0.0	6.967566442713598E-4	1.0064262639475196E-4
28-29	7.741740491903998E-6	5.186966129575679E-4	0.0	0.002078657322076223	1.0064262639475196E-4
30-31	7.741740491903998E-6	6.309518500901758E-4	0.0	0.007203689527716669	1.0064262639475196E-4
32-33	1.5483480983807996E-5	6.464353310739838E-4	0.0	0.019276933824840956	1.0064262639475196E-4
34-35	1.9354351229759996E-5	7.432070872227838E-4	0.0	0.0347642856788949	1.1225523713260797E-4
36-37	2.3225221475711992E-5	8.438497136175358E-4	0.0	0.05817143805616664	1.1612610737855996E-4
38-39	2.3225221475711992E-5	8.709458053391998E-4	0.0	0.10775728590681173	1.1612610737855996E-4
40-41	3.096696196761599E-5	9.832010424718077E-4	0.0	0.15819859608181225	1.1612610737855996E-4
42-43	3.096696196761599E-5	0.0010064262639475196	0.0	0.19839758358602375	1.1612610737855996E-4
44-45	3.096696196761599E-5	0.0011690028142775036	0.0	0.24720151564698656	1.2386784787046394E-4
46-47	3.096696196761599E-5	0.0013044832728858237	0.0	0.2952428862694968	1.4322219910022397E-4
48-49	3.096696196761599E-5	0.0013083541431317756	0.0	0.3393862905543334	1.5483480983807994E-4
50-51	3.096696196761599E-5	0.0014283511207562876	0.0	0.3765621283964564	1.5483480983807994E-4
52-53	3.096696196761599E-5	0.0014399637314941435	0.0	0.4404701961571239	1.7418916106783994E-4
54-55	3.096696196761599E-5	0.0014515763422319997	0.0	0.5048621226985354	1.9741438254355193E-4
56-57	3.096696196761599E-5	0.0014631889529698556	0.0	0.5653405994212894	2.0128525278950392E-4
58-59	3.096696196761599E-5	0.0014709306934617594	0.0	0.6038712418494956	2.0128525278950392E-4
60-61	3.096696196761599E-5	0.0014902850446915196	0.0	0.629887360772539	2.0902699328140793E-4
62-63	3.096696196761599E-5	0.0015212520066591355	0.0	0.6504184565570683	2.2451047426521592E-4
64-65	3.096696196761599E-5	0.0016876994272350715	0.0	0.6567163624472323	2.2451047426521592E-4
66-67	3.096696196761599E-5	0.0016993120379729275	0.0	0.6623213825633708	2.2451047426521592E-4
68-69	3.096696196761599E-5	0.0017341498701864953	0.0	0.6679186609390174	2.2451047426521592E-4
70-71	3.096696196761599E-5	0.0017651168321541114	0.0	0.6727456361357195	2.3225221475711992E-4
72-73	3.096696196761599E-5	0.0017922129238757754	0.0	0.6767713411915095	2.7096091721663993E-4
74-75	3.096696196761599E-5	0.0018618885883029115	0.0	0.6799183587014686	2.7096091721663993E-4
76-77	3.096696196761599E-5	0.0019199516419921912	0.0	0.6833208536476604	2.7096091721663993E-4
78-79	3.096696196761599E-5	0.0019393059932219514	0.0	0.6854769283746557	2.7096091721663993E-4
80-81	3.096696196761599E-5	0.0019547894742057595	0.0	0.686181426759419	2.787026577085439E-4
82-83	3.096696196761599E-5	0.0019664020849436154	0.0	0.6868394747012307	2.941861386923519E-4
84-85	3.096696196761599E-5	0.0019818855659274235	0.0	0.6870872103969716	2.941861386923519E-4
86-87	3.096696196761599E-5	0.002012852527895039	0.0	0.6872110782448422	2.941861386923519E-4
88	3.096696196761599E-5	0.0020902699328140795	0.0	0.6872188199853341	2.941861386923519E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14485	0.0	49.945286	1
GTATCAA	26310	0.0	37.11009	1
TCAACGC	32170	0.0	29.749134	4
ATCAACG	32305	0.0	29.584873	3
CAACGCA	32945	0.0	29.0097	5
TATCAAC	33430	0.0	28.78171	2
AACGCAG	33970	0.0	28.208485	6
ACGCAGA	39345	0.0	24.333662	7
CGCAGAG	40500	0.0	23.629356	8
GTACATG	37745	0.0	21.913254	1
TACATGG	38095	0.0	21.249006	2
ACATGGG	38815	0.0	20.445238	3
GCAGAGT	47360	0.0	20.061695	9
GTGGTAT	7990	0.0	19.48717	1
TGGTATC	7655	0.0	18.822903	2
GAGTACT	27310	0.0	18.599304	12-13
CAGAGTA	46460	0.0	17.880957	10-11
CATGGGG	28760	0.0	17.782318	4
AGAGTAC	42920	0.0	17.762403	10-11
GTACTTT	29850	0.0	16.95158	14-15
>>END_MODULE