FastQCFastQC Report
Fri 27 May 2016
SRR2139542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8546136
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107350.052.433021
GTATCAA195700.038.5867081
TCAACGC242650.030.4559844
CAACGCA248000.029.8865225
ATCAACG247850.029.8170053
TATCAAC252650.029.4401782
AACGCAG253700.029.230186
ACGCAGA295200.025.1068927
CGCAGAG305800.024.2082338
GTACATG280100.023.1305711
TACATGG283550.022.433982
ACATGGG284350.021.7423363
GCAGAGT351500.021.0113289
GAGTACT198050.020.09815812-13
GTGGTAT51950.019.470771
AGAGTAC318550.019.0170810-11
CAGAGTA339200.018.97756610-11
CATGGGG216150.018.9607644
GTACTTT220800.018.0570814-15
CTTATAC67450.017.969811
Produced by FastQC (version 0.11.5)