##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139542_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8546136
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51385093801456	33.0	33.0	33.0	33.0	33.0
2	32.51224986356407	33.0	33.0	33.0	33.0	33.0
3	32.51338148608915	33.0	33.0	33.0	33.0	33.0
4	36.40152637402447	37.0	37.0	37.0	37.0	37.0
5	36.4229631964668	37.0	37.0	37.0	37.0	37.0
6	36.418362403781074	37.0	37.0	37.0	37.0	37.0
7	36.389504215706374	37.0	37.0	37.0	37.0	37.0
8	36.3745418982333	37.0	37.0	37.0	37.0	37.0
9	36.420706972133374	37.0	37.0	37.0	37.0	37.0
10-11	36.35896011952068	37.0	37.0	37.0	37.0	37.0
12-13	36.33955883688254	37.0	37.0	37.0	37.0	37.0
14-15	38.240143849805335	40.0	37.0	40.0	37.0	40.0
16-17	38.19048567680177	40.0	37.0	40.0	35.0	40.0
18-19	38.19272312071794	40.0	37.0	40.0	33.0	40.0
20-21	38.18526910184663	40.0	37.0	40.0	33.0	40.0
22-23	38.19332327985006	40.0	37.0	40.0	35.0	40.0
24-25	38.171401372927676	40.0	37.0	40.0	35.0	40.0
26-27	38.038792739643	40.0	37.0	40.0	33.0	40.0
28-29	37.97024968303552	40.0	37.0	40.0	33.0	40.0
30-31	37.91061171254661	40.0	37.0	40.0	33.0	40.0
32-33	37.84437999487258	40.0	37.0	40.0	33.0	40.0
34-35	37.79243620728191	40.0	37.0	40.0	33.0	40.0
36-37	37.732270953515155	40.0	37.0	40.0	33.0	40.0
38-39	37.673427215802064	40.0	37.0	40.0	33.0	40.0
40-41	37.64315927076268	40.0	37.0	40.0	33.0	40.0
42-43	37.57154617290581	40.0	37.0	40.0	33.0	40.0
44-45	37.47675389185683	40.0	37.0	40.0	33.0	40.0
46-47	37.4246928028529	40.0	37.0	40.0	33.0	40.0
48-49	37.41363076850219	40.0	37.0	40.0	33.0	40.0
50-51	37.307389761013305	40.0	37.0	40.0	33.0	40.0
52-53	37.17418560021261	40.0	37.0	40.0	33.0	40.0
54-55	37.030978435881934	37.0	37.0	40.0	33.0	40.0
56-57	36.90130706778221	37.0	37.0	40.0	33.0	40.0
58-59	36.75612347257999	37.0	37.0	40.0	33.0	40.0
60-61	36.58684117431591	37.0	37.0	40.0	33.0	40.0
62-63	36.39049649272158	37.0	37.0	40.0	33.0	40.0
64-65	36.22216462310936	37.0	37.0	40.0	33.0	40.0
66-67	35.98925858353644	37.0	37.0	40.0	33.0	40.0
68-69	35.78601442036825	37.0	33.0	38.5	33.0	40.0
70-71	35.534858862619586	37.0	33.0	37.0	33.0	40.0
72-73	35.32502977473767	37.0	33.0	37.0	33.0	40.0
74-75	35.12675145008177	37.0	33.0	37.0	30.0	40.0
76-77	33.32956560992764	35.0	33.0	37.0	27.0	37.0
78-79	34.61815560055265	37.0	33.0	37.0	27.0	37.0
80-81	34.730526244336616	37.0	33.0	37.0	33.0	37.0
82-83	34.59404258181234	37.0	33.0	37.0	30.0	37.0
84-85	34.436416729518776	37.0	33.0	37.0	27.0	37.0
86-87	34.305199150522924	37.0	33.0	37.0	27.0	37.0
88-89	34.18218820928716	37.0	33.0	37.0	27.0	37.0
90-91	34.119551950382984	37.0	33.0	37.0	27.0	37.0
92-93	34.085357647795576	37.0	33.0	37.0	27.0	37.0
94-95	34.10242368231516	37.0	33.0	37.0	27.0	37.0
96-97	34.06910728175673	37.0	33.0	37.0	27.0	37.0
98-99	34.104009932111026	37.0	33.0	37.0	27.0	37.0
100	34.11011530647256	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	15.0
20	79.0
21	412.0
22	1760.0
23	4972.0
24	11253.0
25	22002.0
26	38015.0
27	59543.0
28	87838.0
29	120555.0
30	161650.0
31	212640.0
32	277063.0
33	364717.0
34	491829.0
35	697593.0
36	1167827.0
37	2466540.0
38	2310857.0
39	48972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.71171626569013	20.00017317767936	14.353621332494592	23.934489224135913
2	14.837301910477438	20.659114247655314	41.65170083883524	22.851883003032015
3	18.47158762743771	25.791457098272247	29.979501847384594	25.757453426905446
4	12.77041690883601	17.72622263681432	38.06363830074291	31.439722153606763
5	13.792230637033654	37.507679467847105	35.04023280518878	13.65985708993046
6	30.755677185572523	37.07881550211698	18.437934991907454	13.727572320403045
7	27.212125715308733	32.25731865925357	22.49931694268813	18.03123868274957
8	24.750752854857446	36.71444030378173	20.66141938298197	17.87338745837885
9	25.804993857402575	16.903206495532032	20.79227395926005	36.49952568780534
10-11	24.71879583801166	26.54375387861505	28.238985464587508	20.49846481878578
12-13	25.47990774912391	23.993418708903132	28.449404686507858	22.077268855465103
14-15	22.55867823020605	25.326217693141995	26.112831917690226	26.00227215896173
16-17	22.17656611128117	28.438606640474713	26.784426318514004	22.600400929730114
18-19	22.210692645190765	27.537538602240826	28.49923052944629	21.752538223122123
20-21	23.011372944393425	26.69716551664097	28.427978763139638	21.86348277582596
22-23	22.72537694231653	26.652407711257442	28.33361789611637	22.288597450309663
24-25	22.489740628072596	26.852976615075296	28.41214130969179	22.24514144716032
26-27	22.360482427237283	26.949351907362303	28.39628471807764	22.293880947322776
28-29	22.247632181748553	26.889412757413467	28.420829222329324	22.442125838508655
30-31	22.583393113342897	26.775454562504102	28.32860367963541	22.312548644517587
32-33	22.379469643368623	26.9917450825436	28.273528005284703	22.355257268803072
34-35	22.495792841452868	27.03981209298717	28.326705919764507	22.13768914579546
36-37	22.534993444418248	26.910789767612965	28.148219894726562	22.405996893242232
38-39	22.442377948978198	27.015193093361844	28.233444237274824	22.308984720385133
40-41	22.664860073562792	26.888655294075285	28.1415847942945	22.304899838067424
42-43	22.57596960322269	26.931637633526528	27.983747160578552	22.508645602672228
44-45	22.59099528414086	27.113329829987915	27.79136673539381	22.50430815047741
46-47	22.796398888209335	27.02905865838179	27.431255352248403	22.743287101160472
48-49	22.669790556675963	27.152442116521403	27.45586476303381	22.721902563768822
50-51	22.61897000936047	27.279817520560677	27.40637198560309	22.694840484475765
52-53	22.801037973439342	27.22013402765241	27.315441736184976	22.66338626272327
54-55	22.78127487620633	27.129692707914433	27.283973684784236	22.805058731095006
56-57	22.80152091405483	27.33176904434711	27.19517145837016	22.6715385832279
58-59	22.70077225779683	27.372077548088665	27.331595739788224	22.59555445432629
60-61	22.78945474972548	27.442334877217455	27.187307007950057	22.580903365107005
62-63	22.756272985855254	27.53847389645476	27.088339822429436	22.616913295260545
64-65	22.770149626536167	27.411115188605894	27.124028043696356	22.694707141161576
66-67	22.818227456919253	27.422763019964542	26.950567538148277	22.808441984967924
68-69	22.708984491680727	27.37875013118698	27.093629560261245	22.81863581687105
70-71	22.736051863261466	27.16412922147235	27.147060372492803	22.95275854277338
72-73	22.807165711407347	27.221143615433085	27.030085189156043	22.941605484003524
74-75	22.641927674373406	27.37213573745793	27.034971061520345	22.95096552664831
76-77	22.825572341026977	27.298572230702927	26.91815436268918	22.95770106558091
78-79	22.777415327593	27.27006572136011	26.91420676308275	23.038312187964138
80-81	22.75541212299526	27.36458038880385	26.90471603877161	22.97529144942928
82-83	22.8178859324766	27.302313973316185	27.028859359048106	22.850940735159107
84-85	22.91497103428337	27.32732381369889	26.900038299714236	22.857666852303506
86-87	22.712784931420618	27.370293691815938	26.994369566281744	22.9225518104817
88-89	22.76333025736628	27.353311063869423	26.9831058044255	22.90025287433879
90-91	22.781550838225623	27.379793587678698	26.98839848052923	22.85025709356645
92-93	22.784825372152355	27.34932012343504	27.022340024476893	22.843514479935713
94-95	22.62420092696007	27.370423812082056	27.07974000819985	22.925635252758024
96-97	22.738152575108668	27.458060324553813	27.117820606960226	22.685966493377293
98-99	23.88528264482695	29.37571022448651	27.27083041833448	19.468176712352054
100	22.817948420299437	30.421176322521358	21.05370640685059	25.70716885032861
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1919.0
1	1625.5
2	2072.0
3	2991.0
4	4005.0
5	6465.5
6	8660.0
7	10325.5
8	11465.5
9	11334.5
10	10433.0
11	9436.0
12	9941.5
13	12818.0
14	20214.0
15	30820.0
16	39693.0
17	45142.5
18	46451.5
19	43625.0
20	38797.0
21	33689.0
22	30271.0
23	30377.0
24	34163.5
25	42026.5
26	53294.0
27	66217.0
28	79880.5
29	96268.5
30	113029.0
31	129724.0
32	150040.0
33	170548.0
34	189430.5
35	207170.5
36	228200.5
37	248437.5
38	261759.0
39	273561.0
40	286900.0
41	298136.0
42	305281.5
43	313659.0
44	322748.5
45	330761.0
46	338825.5
47	347140.5
48	351714.0
49	352659.0
50	353117.5
51	349164.5
52	338293.5
53	323576.0
54	308127.0
55	288954.0
56	266386.5
57	241448.0
58	216104.0
59	190027.0
60	159870.0
61	130461.5
62	103824.0
63	79518.5
64	58792.5
65	42850.5
66	31837.5
67	24137.5
68	18109.0
69	12917.0
70	8962.0
71	6203.0
72	4103.0
73	2467.5
74	1494.0
75	973.0
76	675.0
77	447.5
78	299.0
79	228.5
80	183.0
81	126.0
82	93.5
83	68.5
84	46.0
85	30.5
86	18.5
87	17.5
88	14.5
89	8.5
90	5.0
91	3.0
92	2.5
93	2.0
94	2.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.001392442151634376
5	0.007278143011063713
6	0.0
7	1.1701194551549379E-5
8	0.0
9	9.009919804693022E-4
10-11	8.951413831935276E-4
12-13	0.0010823604960183174
14-15	9.243943695724008E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	155.0
20-21	496.0
22-23	995.0
24-25	2600.0
26-27	3240.0
28-29	5020.0
30-31	10325.0
32-33	12819.0
34-35	18257.0
36-37	24794.0
38-39	30079.0
40-41	34376.0
42-43	42973.0
44-45	52956.0
46-47	51396.0
48-49	46249.0
50-51	40401.0
52-53	39215.0
54-55	45344.0
56-57	48627.0
58-59	51618.0
60-61	53197.0
62-63	53966.0
64-65	58757.0
66-67	61507.0
68-69	60651.0
70-71	61472.0
72-73	60643.0
74-75	63044.0
76-77	61794.0
78-79	67819.0
80-81	71243.0
82-83	70276.0
84-85	72229.0
86-87	77589.0
88-89	84353.0
90-91	90578.0
92-93	97165.0
94-95	120530.0
96-97	310611.0
98-99	554754.0
100-101	5832023.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.34848865710019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.34223854198262	72.74849828517294
2	7.9458725530246594	13.086611916070298
3	1.994052999648245	4.926217524695704
4	0.749524813641862	2.46888942457608
5	0.35693776438846186	1.4696642721016977
6	0.1962188565860432	0.9694995771530968
7	0.11532512690495826	0.6647794933368117
8	0.0686663328187812	0.4523654983401657
9	0.04526409298254498	0.33546866827923305
>10	0.17918010946122673	2.3226608507264532
>50	0.005000592642308956	0.27636909120566705
>100	0.0015838205304042653	0.19209757263205327
>500	1.0751643931809402E-4	0.0554691008501478
>1k	2.6878948595062474E-5	0.03140872485969804
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1701194551549379E-5	2.3402389103098758E-5
2	0.0	5.8505972757746895E-5	0.0	1.1701194551549379E-5	2.3402389103098758E-5
3	0.0	5.8505972757746895E-5	0.0	2.3402389103098758E-5	2.3402389103098758E-5
4	0.0	1.5211552917014193E-4	0.0	4.6804778206197516E-5	3.510358365464814E-5
5	0.0	1.5211552917014193E-4	0.0	4.6804778206197516E-5	3.510358365464814E-5
6	0.0	1.5211552917014193E-4	0.0	5.8505972757746895E-5	3.510358365464814E-5
7	0.0	1.5211552917014193E-4	0.0	5.8505972757746895E-5	4.6804778206197516E-5
8	0.0	1.638167237216913E-4	0.0	8.190836186084565E-5	5.8505972757746895E-5
9	0.0	3.5103583654648137E-4	0.0	1.0531075096394441E-4	8.190836186084565E-5
10-11	0.0	3.5103583654648137E-4	0.0	1.5796612644591662E-4	8.190836186084565E-5
12-13	0.0	3.5103583654648137E-4	0.0	1.8721911282479006E-4	8.775895913662034E-5
14-15	0.0	4.7974897661352454E-4	0.0	2.0477090465211413E-4	1.5796612644591662E-4
16-17	0.0	4.914501711650739E-4	0.0	2.223226964794382E-4	1.8721911282479006E-4
18-19	0.0	5.26553754819722E-4	0.0	2.3987448830676227E-4	2.0477090465211413E-4
20-21	0.0	5.733585330259196E-4	0.0	2.808286692371851E-4	2.223226964794382E-4
22-23	0.0	6.143127139563424E-4	0.0	3.451852392707067E-4	2.691274746856357E-4
24-25	0.0	6.845198812656387E-4	0.0	4.0369121202845357E-4	3.276334474433826E-4
26-27	0.0	7.137728676445121E-4	0.0	4.446453929588764E-4	3.276334474433826E-4
28-29	0.0	7.254740621960615E-4	0.0	6.96221075817188E-4	3.334840447191573E-4
30-31	0.0	8.307848131600059E-4	0.0	0.0013982927489101507	3.451852392707067E-4
32-33	0.0	8.424860077115553E-4	0.0	0.0032880356689853757	3.6273703109803075E-4
34-35	0.0	0.0010355557178121201	0.0	0.005499561439228208	3.744382256495801E-4
36-37	0.0	0.0012227748306369102	0.0	0.009437013405824575	3.744382256495801E-4
38-39	0.0	0.0012754302061188823	0.0	0.01941228176102042	3.919900174769042E-4
40-41	0.0	0.001392442151634376	0.0	0.030885303018814585	3.978406147526789E-4
42-43	0.0	0.0014860517080467711	0.0	0.038584689033734076	3.978406147526789E-4
44-45	0.0	0.0017200755990777587	0.0	0.04788128810494006	4.0369121202845357E-4
46-47	0.0	0.0018370875445932525	0.0	0.05815493692120041	4.2709360113155233E-4
48-49	0.0	0.0018487887391448019	0.0	0.06763290450795541	4.563465875104258E-4
50-51	0.0	0.0020945138247273386	0.0	0.0775028621121873	4.7974897661352454E-4
52-53	0.0	0.0021179162138304374	0.0	0.09943090070179084	5.382549493712714E-4
54-55	0.0	0.002141318602933536	0.0	0.12591655456922288	6.02611519404793E-4
56-57	0.0	0.0021881233811397336	0.0	0.15591256680211968	6.260139085078918E-4
58-59	0.0	0.0021881233811397336	0.0	0.18386672058577114	6.435657003352158E-4
60-61	0.0	0.0022115257702428324	0.0	0.19500040720157039	6.611174921625399E-4
62-63	0.0	0.002270031743000579	0.0	0.2094455318754581	6.903704785414134E-4
64-65	0.0	0.002656171163201709	0.0	0.2148514837582739	6.96221075817188E-4
66-67	0.0	0.0026912747468563567	0.0	0.21919262693689873	7.137728676445121E-4
68-69	0.0	0.0027614819141656534	0.0	0.2237092880337968	7.254740621960615E-4
70-71	0.0	0.0027848843032687517	0.0	0.22750632566577456	7.605776458507096E-4
72-73	0.0	0.002802436095096076	0.0	0.23088797089117233	8.65888396814654E-4
74-75	0.0	0.0029077468460600208	0.0	0.23371965997264726	8.717389940904287E-4
76-77	0.0	0.003030609388851289	0.0	0.23664495861053464	8.775895913662034E-4
78-79	0.0	0.003089115361609036	0.0	0.23858735690609184	8.834401886419781E-4
80-81	0.0	0.003100816556160585	0.0	0.23923677320370285	9.009919804693022E-4
82-83	0.0	0.00310666715343636	0.0	0.23961706202662816	9.594979532270491E-4
84-85	0.0	0.003147621334366783	0.0	0.23988033890403804	9.594979532270491E-4
86-87	5.8505972757746895E-6	0.003211977904400304	0.0	0.23996224726589888	9.594979532270491E-4
88	1.1701194551549379E-5	0.0033582428362946715	0.0	0.23996809786317466	9.594979532270491E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10735	0.0	52.43302	1
GTATCAA	19570	0.0	38.586708	1
TCAACGC	24265	0.0	30.455984	4
CAACGCA	24800	0.0	29.886522	5
ATCAACG	24785	0.0	29.817005	3
TATCAAC	25265	0.0	29.440178	2
AACGCAG	25370	0.0	29.23018	6
ACGCAGA	29520	0.0	25.106892	7
CGCAGAG	30580	0.0	24.208233	8
GTACATG	28010	0.0	23.130571	1
TACATGG	28355	0.0	22.43398	2
ACATGGG	28435	0.0	21.742336	3
GCAGAGT	35150	0.0	21.011328	9
GAGTACT	19805	0.0	20.098158	12-13
GTGGTAT	5195	0.0	19.47077	1
AGAGTAC	31855	0.0	19.01708	10-11
CAGAGTA	33920	0.0	18.977566	10-11
CATGGGG	21615	0.0	18.960764	4
GTACTTT	22080	0.0	18.05708	14-15
CTTATAC	6745	0.0	17.96981	1
>>END_MODULE