FastQCFastQC Report
Fri 27 May 2016
SRR2139541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7895533
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103950.053.0503731
GTATCAA194050.038.8479461
CTTATAC71300.038.5822641
TATACAC95500.031.7444173
TCAACGC242750.030.4944464
ATCAACG245600.030.0886443
TATCAAC248700.029.935282
CAACGCA247950.029.8377695
AACGCAG257250.028.8061166
TTATACA103500.027.4826562
ACGCAGA295550.025.073327
CGCAGAG303250.024.4087838
GTGGTAT48250.022.823141
GTACATG272700.022.3114341
ACACATC126900.021.8438226
TACACAT135750.021.7057385
TACATGG274200.021.6776732
ACATCTC131700.021.33858
TGGTATC47100.021.3041422
GCAGAGT345550.021.2489729
Produced by FastQC (version 0.11.5)