##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139541_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7895533 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49774131778057 33.0 33.0 33.0 33.0 33.0 2 32.50058735743363 33.0 33.0 33.0 33.0 33.0 3 32.500101766403866 33.0 33.0 33.0 33.0 33.0 4 36.38982726055353 37.0 37.0 37.0 37.0 37.0 5 36.41126444535157 37.0 37.0 37.0 37.0 37.0 6 36.40374563693167 37.0 37.0 37.0 37.0 37.0 7 36.37659902124404 37.0 37.0 37.0 37.0 37.0 8 36.36246748636223 37.0 37.0 37.0 37.0 37.0 9 36.40865930140498 37.0 37.0 37.0 37.0 37.0 10-11 36.346854860843465 37.0 37.0 37.0 37.0 37.0 12-13 36.32457707415067 37.0 37.0 37.0 35.0 37.0 14-15 38.203716392547534 40.0 37.0 40.0 35.0 40.0 16-17 38.1510076647137 40.0 37.0 40.0 33.0 40.0 18-19 38.156016129626714 40.0 37.0 40.0 33.0 40.0 20-21 38.14755761330693 40.0 37.0 40.0 33.0 40.0 22-23 38.155686995669114 40.0 37.0 40.0 33.0 40.0 24-25 38.13246314052029 40.0 37.0 40.0 33.0 40.0 26-27 37.992560437245054 40.0 37.0 40.0 33.0 40.0 28-29 37.91809319238857 40.0 37.0 40.0 33.0 40.0 30-31 37.85369164510355 40.0 37.0 40.0 33.0 40.0 32-33 37.78655206448519 40.0 37.0 40.0 33.0 40.0 34-35 37.73015972214829 40.0 37.0 40.0 33.0 40.0 36-37 37.670934528947335 40.0 37.0 40.0 33.0 40.0 38-39 37.60459812885611 40.0 37.0 40.0 33.0 40.0 40-41 37.591157123611865 40.0 37.0 40.0 33.0 40.0 42-43 37.52229308724649 40.0 37.0 40.0 33.0 40.0 44-45 37.4284863518595 40.0 37.0 40.0 33.0 40.0 46-47 37.382482026030445 40.0 37.0 40.0 33.0 40.0 48-49 37.377961258638095 40.0 37.0 40.0 33.0 40.0 50-51 37.26944596213825 40.0 37.0 40.0 33.0 40.0 52-53 37.14512351545785 40.0 37.0 40.0 33.0 40.0 54-55 37.00325407341072 37.0 37.0 40.0 33.0 40.0 56-57 36.87918907803235 37.0 37.0 40.0 33.0 40.0 58-59 36.736668301886525 37.0 37.0 40.0 33.0 40.0 60-61 36.56782697193982 37.0 37.0 40.0 33.0 40.0 62-63 36.37815831543516 37.0 37.0 40.0 33.0 40.0 64-65 36.22081830465881 37.0 37.0 40.0 33.0 40.0 66-67 35.99056838042057 37.0 37.0 40.0 33.0 40.0 68-69 35.79267087313807 37.0 33.0 38.5 33.0 40.0 70-71 35.546331294362574 37.0 33.0 37.0 33.0 40.0 72-73 35.335128244093056 37.0 33.0 37.0 33.0 40.0 74-75 35.142403333931796 37.0 33.0 37.0 33.0 40.0 76-77 33.335782463071695 35.0 33.0 37.0 27.0 37.0 78-79 34.63169849358649 37.0 33.0 37.0 27.0 37.0 80-81 34.74678908086434 37.0 33.0 37.0 33.0 37.0 82-83 34.61180268762319 37.0 33.0 37.0 30.0 37.0 84-85 34.45323116551553 37.0 33.0 37.0 27.0 37.0 86-87 34.32447082827362 37.0 33.0 37.0 27.0 37.0 88-89 34.20074951416923 37.0 33.0 37.0 27.0 37.0 90-91 34.14104865623127 37.0 33.0 37.0 27.0 37.0 92-93 34.10267802149592 37.0 33.0 37.0 27.0 37.0 94-95 34.12068195265894 37.0 33.0 37.0 27.0 37.0 96-97 34.083675396078384 37.0 33.0 37.0 27.0 37.0 98-99 34.117834021250076 37.0 33.0 37.0 27.0 37.0 100 34.12881699694306 37.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 2.0 19 9.0 20 84.0 21 468.0 22 1647.0 23 4674.0 24 10346.0 25 20138.0 26 34951.0 27 54427.0 28 79970.0 29 111422.0 30 149998.0 31 197319.0 32 259031.0 33 339779.0 34 456480.0 35 647627.0 36 1074369.0 37 2248209.0 38 2150281.0 39 54301.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.37302700147033 20.24766409056868 14.514966880639976 23.864342027321015 2 14.745894925649731 20.71280051644392 41.8127313254216 22.72857323248475 3 18.17485912604 26.156942159573017 30.373566927020633 25.29463178736635 4 12.63160001220961 18.009083999447277 38.04072005786645 31.31859593047667 5 13.539864224788445 37.71589245342694 35.15639912597415 13.587844195810467 6 30.26626574798687 37.39191514999684 18.4320298578956 13.909789244120693 7 26.849308336751932 32.60327073549056 22.404845879309224 18.142575048448283 8 24.655954195872525 36.71550736346742 20.737371371888383 17.891167068771672 9 25.679418329530098 17.000919641612082 20.768575183709842 36.551086845147985 10-11 24.568907485222844 26.625265674729903 28.291767056599465 20.514059783447784 12-13 25.412233435381747 24.06718413442805 28.416747879201846 22.103834550988353 14-15 22.55680541556656 25.444590829517004 26.048804997098333 25.949798757818098 16-17 22.10639864338481 28.644430971284653 26.69368236444582 22.55548802088472 18-19 22.125295404376118 27.58949902432173 28.536401532360134 21.74880403894202 20-21 23.04242791242066 26.711327782653736 28.41898724806165 21.827257056863953 22-23 22.70900445766736 26.812827371030128 28.279232985319798 22.19893518598272 24-25 22.453006420373402 27.053599396698374 28.47184597691757 22.021548206010657 26-27 22.42332986039075 27.149886008823497 28.46993792426687 21.956846206518886 28-29 22.254541272471148 26.91702444698508 28.656769086968975 22.171665193574796 30-31 22.671054123868533 26.95615523591605 28.31928118747196 22.053509452743455 32-33 22.4488984844842 26.973934003243123 28.377019568049416 22.20014794422326 34-35 22.306211962690128 27.188680866667365 28.595291565295465 21.909815605347045 36-37 22.546901749777533 26.966990644569456 28.273106737536313 22.2130008681167 38-39 22.26809481691911 27.030354794985488 28.455988524656156 22.245561863439242 40-41 22.50690681616117 26.895899530257594 28.370026312974204 22.227167340607036 42-43 22.527846986434856 26.93301770149603 28.20377132046342 22.33536399160569 44-45 22.422856176961968 27.21328284343791 27.968328087293305 22.39553289230682 46-47 22.744355998247116 26.971058462469976 27.503856783641755 22.780728755641157 48-49 22.583264682752834 27.071048975972005 27.589110947041384 22.75657539423378 50-51 22.44962851713044 27.289893172250153 27.57564508271617 22.68483322790324 52-53 22.635396529853036 27.265851208013842 27.57094120674905 22.527811055384074 54-55 22.634535023968894 27.177759327025907 27.409562272995956 22.77814337600924 56-57 22.75249750842063 27.49212401694991 27.142089518158606 22.613288956470853 58-59 22.527408763039592 27.632982716570936 27.435952988939366 22.403655531450102 60-61 22.743304294392942 27.78735299743243 27.07571246412629 22.39363024404834 62-63 22.591754359958948 27.912113554332013 27.042504856613753 22.453627229095282 64-65 22.64680006355189 27.661246084058977 27.112172665152173 22.579781187236957 66-67 22.761937261590802 27.581264802806913 26.933511642943554 22.723286292658734 68-69 22.655097535790052 27.53894085100006 27.093577459643996 22.712384153565893 70-71 22.69718874991529 27.25804588599468 27.191875176946045 22.852890187143977 72-73 22.78680743984454 27.33224977388532 27.079150584913155 22.80179220135699 74-75 22.53960942056519 27.521837008252454 27.084648549105573 22.85390502207679 76-77 22.700091592059 27.445643799646763 27.032874601971745 22.821390006322492 78-79 22.702691443884834 27.4359661952207 26.94713470469931 22.914207656195153 80-81 22.675788373460172 27.47888012732762 26.95356976857844 22.891761730633764 82-83 22.77748843522386 27.389736196847775 27.08616352384085 22.746611844087514 84-85 22.84457659460072 27.38142130098376 26.967011733939415 22.8069903704761 86-87 22.6163055315754 27.51602340712972 27.05703991480428 22.810631146490596 88-89 22.740539224083864 27.4283801070522 27.03354357550961 22.797537093354325 90-91 22.721578344425414 27.492774923874542 27.055453366107233 22.73019336559281 92-93 22.790707787904065 27.467983014595653 27.031413798654803 22.70989539884548 94-95 22.60465211113787 27.483511082735774 27.090784514371812 22.821052291754544 96-97 22.669683513283427 27.585765890213583 27.17217080549718 22.572379791005808 98-99 23.816344844571393 29.429958282495726 27.330494920013926 19.42320195291895 100 22.734684157798668 30.539301105610516 21.09714221465349 25.628872521937325 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2386.0 1 2024.5 2 2351.5 3 3236.0 4 4239.5 5 6638.0 6 8678.0 7 10172.0 8 11348.0 9 11449.5 10 10721.5 11 9868.0 12 10659.0 13 13714.5 14 21107.5 15 31718.0 16 40780.5 17 46666.0 18 48319.0 19 45658.0 20 40728.0 21 35465.0 22 31985.0 23 32171.0 24 36357.0 25 44449.0 26 56184.5 27 69586.0 28 82105.0 29 97033.0 30 112019.5 31 126491.5 32 145828.5 33 165921.5 34 185494.5 35 203382.0 36 223794.0 37 242499.5 38 253101.0 39 263431.0 40 275505.5 41 284108.0 42 288134.5 43 295955.0 44 304594.5 45 310993.5 46 321123.0 47 331300.0 48 334141.5 49 335095.5 50 336558.0 51 331499.5 52 321104.0 53 304966.0 54 287492.0 55 271105.5 56 251275.0 57 228357.5 58 204809.0 59 180645.5 60 152567.0 61 124043.5 62 98327.0 63 75098.5 64 55601.0 65 41158.5 66 30583.5 67 22950.0 68 17376.5 69 12465.5 70 8673.0 71 5909.5 72 3848.0 73 2476.5 74 1651.5 75 1119.5 76 762.5 77 494.5 78 333.5 79 232.5 80 159.0 81 117.5 82 90.0 83 62.0 84 41.0 85 28.5 86 22.5 87 16.5 88 8.5 89 6.0 90 6.5 91 6.0 92 5.0 93 3.5 94 1.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0014185236132886786 5 0.007244602739295751 6 0.0 7 0.0 8 0.0 9 8.359157006879714E-4 10-11 8.865772583054241E-4 12-13 0.0012348754669254123 14-15 8.992426477097874E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 137.0 20-21 464.0 22-23 1045.0 24-25 2559.0 26-27 3502.0 28-29 6364.0 30-31 14222.0 32-33 18273.0 34-35 25169.0 36-37 37310.0 38-39 44733.0 40-41 47482.0 42-43 57904.0 44-45 68370.0 46-47 66495.0 48-49 60847.0 50-51 54722.0 52-53 54306.0 54-55 62200.0 56-57 66334.0 58-59 67457.0 60-61 69178.0 62-63 68625.0 64-65 73949.0 66-67 74109.0 68-69 67066.0 70-71 66559.0 72-73 63744.0 74-75 65773.0 76-77 64071.0 78-79 69633.0 80-81 71559.0 82-83 68988.0 84-85 70695.0 86-87 75252.0 88-89 80381.0 90-91 85347.0 92-93 90159.0 94-95 109249.0 96-97 270216.0 98-99 479306.0 100-101 5051779.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.57403784901781 #Duplication Level Percentage of deduplicated Percentage of total 1 89.11911097714432 74.48043953874678 2 7.448548212859846 12.450105005235654 3 1.8713804792752193 4.6919646901458085 4 0.7086878417086061 2.3691161802437515 5 0.30919991953625026 1.292054288911792 6 0.17760961805769362 0.8906131765141971 7 0.09775457446843519 0.5718821154577743 8 0.06634934888042883 0.4436066395672517 9 0.04411599540284879 0.33182566825903026 >10 0.1524800076588023 1.9991849689360082 >50 0.003341456811843166 0.18887136206263092 >100 0.0012606663115090253 0.16816838683204044 >500 1.0726794429777813E-4 0.05730757986550946 >1k 5.363393988609711E-5 0.06486039922178456 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.5330778808726402E-5 0.0 1.2665389404363201E-5 0.0 3 0.0 2.5330778808726402E-5 0.0 1.2665389404363201E-5 0.0 4 0.0 1.2665389404363202E-4 0.0 1.2665389404363201E-5 0.0 5 0.0 1.2665389404363202E-4 0.0 1.2665389404363201E-5 0.0 6 1.1398850463926882E-4 1.2665389404363202E-4 0.0 1.2665389404363201E-5 0.0 7 1.1398850463926882E-4 1.3931928344799522E-4 0.0 1.2665389404363201E-5 0.0 8 1.1398850463926882E-4 1.519846728523584E-4 0.0 1.2665389404363201E-5 0.0 9 1.1398850463926882E-4 2.4064239868290082E-4 0.0 1.2665389404363201E-5 0.0 10-11 1.1398850463926882E-4 2.4064239868290082E-4 0.0 5.69942523196344E-5 0.0 12-13 1.1398850463926882E-4 2.4064239868290082E-4 0.0 7.59923364261792E-5 0.0 14-15 1.1398850463926882E-4 3.7996168213089604E-4 0.0 7.59923364261792E-5 0.0 16-17 1.519846728523584E-4 3.7996168213089604E-4 0.0 8.865772583054241E-5 0.0 18-19 1.646500622567216E-4 4.0529246093962243E-4 0.0 8.865772583054241E-5 0.0 20-21 1.7098275695890322E-4 4.3695593445053046E-4 0.0 8.865772583054241E-5 0.0 22-23 1.836481463632664E-4 4.496213238548936E-4 0.0 8.865772583054241E-5 0.0 24-25 1.8998084106544802E-4 5.129482708767097E-4 0.0 8.865772583054241E-5 1.2665389404363201E-5 26-27 1.8998084106544802E-4 5.446117443876177E-4 0.0 1.26653894043632E-4 1.2665389404363201E-5 28-29 1.8998084106544802E-4 5.446117443876177E-4 0.0 2.913039563003536E-4 1.2665389404363201E-5 30-31 1.8998084106544802E-4 5.952733020050705E-4 0.0 9.309061212206953E-4 1.2665389404363201E-5 32-33 1.8998084106544802E-4 5.952733020050705E-4 0.0 0.002191112366954834 1.2665389404363201E-5 34-35 1.8998084106544802E-4 7.345925854530656E-4 0.0 0.0039516014941613185 1.2665389404363201E-5 36-37 1.8998084106544802E-4 8.739118689010609E-4 0.0 0.00672532177371686 1.2665389404363201E-5 38-39 1.8998084106544802E-4 9.055753424119688E-4 0.0 0.01099989069768944 1.2665389404363201E-5 40-41 1.8998084106544802E-4 9.942330682425112E-4 0.0 0.015407446210407835 1.2665389404363201E-5 42-43 1.8998084106544802E-4 0.0010258965417534191 0.0 0.019359047704569154 1.2665389404363201E-5 44-45 1.8998084106544802E-4 0.0011905466040101407 0.0 0.02401357831067263 2.5330778808726402E-5 46-47 1.8998084106544802E-4 0.0013172004980537728 0.0 0.028623780053860834 2.5330778808726402E-5 48-49 1.8998084106544802E-4 0.0013235331927559544 0.0 0.03346829150102976 2.5330778808726402E-5 50-51 2.1531161987417444E-4 0.0015768409808432186 0.0 0.03829380486409214 2.5330778808726402E-5 52-53 2.2797700927853763E-4 0.0015958390649497634 0.0 0.04282168157615199 2.5330778808726402E-5 54-55 2.2797700927853763E-4 0.0016148371490563081 0.0 0.04762186416040563 2.5330778808726402E-5 56-57 2.2797700927853763E-4 0.0016211698437584897 0.0 0.05292866232083382 2.5330778808726402E-5 58-59 2.2797700927853763E-4 0.0016401679278650345 0.0 0.0585774259951798 2.5330778808726402E-5 60-61 2.53307788087264E-4 0.0016591660119715794 0.0 0.06390955493441672 2.5330778808726402E-5 62-63 2.9130395630035366E-4 0.0016844967907803058 0.0 0.06909603189550345 2.5330778808726402E-5 64-65 2.9130395630035366E-4 0.0018491468530370274 0.0 0.07432683771950543 2.5330778808726402E-5 66-67 3.1663473510908004E-4 0.0018744776318457537 0.0 0.07990594175212744 2.5330778808726402E-5 68-69 3.1663473510908004E-4 0.001887143021250117 0.0 0.08574468626753887 2.5330778808726402E-5 70-71 3.293001245134432E-4 0.001887143021250117 0.0 0.09084250550279507 2.5330778808726402E-5 72-73 3.7996168213089604E-4 0.0018998084106544803 0.0 0.09492076089100002 2.5330778808726402E-5 74-75 3.7996168213089604E-4 0.0019694680523784777 0.0 0.09794145626394063 2.5330778808726402E-5 76-77 3.7996168213089604E-4 0.0020707911676133835 0.0 0.10096848433158344 2.5330778808726402E-5 78-79 3.7996168213089604E-4 0.0021024546411242914 0.0 0.10312160053032518 2.5330778808726402E-5 80-81 3.7996168213089604E-4 0.0021024546411242914 0.0 0.10396384892571534 2.5330778808726402E-5 82-83 3.7996168213089604E-4 0.0021024546411242914 0.0 0.10464144725884877 2.5330778808726402E-5 84-85 3.7996168213089604E-4 0.0021277854199330177 0.0 0.10494541660455348 2.5330778808726402E-5 86-87 3.7996168213089604E-4 0.002178446977550471 0.0 0.1050784031932993 2.5330778808726402E-5 88 3.7996168213089604E-4 0.002191112366954834 0.0 0.10508473588800148 2.5330778808726402E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10395 0.0 53.050373 1 GTATCAA 19405 0.0 38.847946 1 CTTATAC 7130 0.0 38.582264 1 TATACAC 9550 0.0 31.744417 3 TCAACGC 24275 0.0 30.494446 4 ATCAACG 24560 0.0 30.088644 3 TATCAAC 24870 0.0 29.93528 2 CAACGCA 24795 0.0 29.837769 5 AACGCAG 25725 0.0 28.806116 6 TTATACA 10350 0.0 27.482656 2 ACGCAGA 29555 0.0 25.07332 7 CGCAGAG 30325 0.0 24.408783 8 GTGGTAT 4825 0.0 22.82314 1 GTACATG 27270 0.0 22.311434 1 ACACATC 12690 0.0 21.843822 6 TACACAT 13575 0.0 21.705738 5 TACATGG 27420 0.0 21.677673 2 ACATCTC 13170 0.0 21.3385 8 TGGTATC 4710 0.0 21.304142 2 GCAGAGT 34555 0.0 21.248972 9 >>END_MODULE