FastQCFastQC Report
Fri 27 May 2016
SRR2139540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7923928
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102300.053.3685841
GTATCAA191000.039.041461
CTTATAC65600.034.536721
TCAACGC238850.030.6855814
ATCAACG244050.030.0841143
TATCAAC245750.030.0315882
CAACGCA246750.029.7037095
AACGCAG253400.028.9552466
TATACAC89350.027.4540653
TTATACA92200.025.9122542
ACGCAGA291200.025.197117
CGCAGAG301400.024.3586718
GTACATG280800.022.855431
TACATGG285750.021.9229852
GCAGAGT343100.021.2864389
ACATGGG291750.021.0636793
GTGGTAT50400.020.5328391
TACACAT120150.020.2749235
GAGTACT186100.020.01043712-13
ACATCTC120450.019.7635338
Produced by FastQC (version 0.11.5)