##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139540_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7923928
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50838006100005	33.0	33.0	33.0	33.0	33.0
2	32.51003997007545	33.0	33.0	33.0	33.0	33.0
3	32.511843747192046	33.0	33.0	33.0	33.0	33.0
4	36.40020807861959	37.0	37.0	37.0	37.0	37.0
5	36.42243543858551	37.0	37.0	37.0	37.0	37.0
6	36.417598317400156	37.0	37.0	37.0	37.0	37.0
7	36.39048663743537	37.0	37.0	37.0	37.0	37.0
8	36.37644764061461	37.0	37.0	37.0	37.0	37.0
9	36.420217472950284	37.0	37.0	37.0	37.0	37.0
10-11	36.35949140375834	37.0	37.0	37.0	37.0	37.0
12-13	36.33818019295481	37.0	37.0	37.0	37.0	37.0
14-15	38.23065000085816	40.0	37.0	40.0	37.0	40.0
16-17	38.17875414819519	40.0	37.0	40.0	33.0	40.0
18-19	38.18098151573311	40.0	37.0	40.0	33.0	40.0
20-21	38.17188257805782	40.0	37.0	40.0	33.0	40.0
22-23	38.178982291430216	40.0	37.0	40.0	33.0	40.0
24-25	38.15860240538901	40.0	37.0	40.0	33.0	40.0
26-27	38.018541559618804	40.0	37.0	40.0	33.0	40.0
28-29	37.94893451352921	40.0	37.0	40.0	33.0	40.0
30-31	37.882225198868014	40.0	37.0	40.0	33.0	40.0
32-33	37.819231542198835	40.0	37.0	40.0	33.0	40.0
34-35	37.77036947015617	40.0	37.0	40.0	33.0	40.0
36-37	37.70447826212451	40.0	37.0	40.0	33.0	40.0
38-39	37.639807560603344	40.0	37.0	40.0	33.0	40.0
40-41	37.616323668819106	40.0	37.0	40.0	33.0	40.0
42-43	37.54747582356203	40.0	37.0	40.0	33.0	40.0
44-45	37.45748203296608	40.0	37.0	40.0	33.0	40.0
46-47	37.40716041889078	40.0	37.0	40.0	33.0	40.0
48-49	37.402867036124746	40.0	37.0	40.0	33.0	40.0
50-51	37.28812321451992	40.0	37.0	40.0	33.0	40.0
52-53	37.16105536904637	40.0	37.0	40.0	33.0	40.0
54-55	37.02231605061111	37.0	37.0	40.0	33.0	40.0
56-57	36.89909109228405	37.0	37.0	40.0	33.0	40.0
58-59	36.76387827686715	37.0	37.0	40.0	33.0	40.0
60-61	36.5876487923732	37.0	37.0	40.0	33.0	40.0
62-63	36.39778714642539	37.0	37.0	40.0	33.0	40.0
64-65	36.23756348947052	37.0	37.0	40.0	33.0	40.0
66-67	36.010770120051035	37.0	37.0	40.0	33.0	40.0
68-69	35.811384209709736	37.0	33.0	38.5	33.0	40.0
70-71	35.564007535725544	37.0	33.0	37.0	33.0	40.0
72-73	35.35703721843805	37.0	33.0	37.0	33.0	40.0
74-75	35.16091900481348	37.0	33.0	37.0	33.0	40.0
76-77	33.342722155295235	35.0	33.0	37.0	27.0	37.0
78-79	34.64718806823686	37.0	33.0	37.0	30.0	37.0
80-81	34.76004863722335	37.0	33.0	37.0	33.0	37.0
82-83	34.630341543453376	37.0	33.0	37.0	33.0	37.0
84-85	34.47567513638273	37.0	33.0	37.0	27.0	37.0
86-87	34.34675950121099	37.0	33.0	37.0	27.0	37.0
88-89	34.227769980407686	37.0	33.0	37.0	27.0	37.0
90-91	34.16607316574462	37.0	33.0	37.0	27.0	37.0
92-93	34.129455642990564	37.0	33.0	37.0	27.0	37.0
94-95	34.15054402684471	37.0	33.0	37.0	27.0	37.0
96-97	34.11361797450515	37.0	33.0	37.0	27.0	37.0
98-99	34.145223977783886	37.0	33.0	37.0	27.0	37.0
100	34.15510116122629	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	12.0
20	91.0
21	445.0
22	1620.0
23	4498.0
24	10179.0
25	19720.0
26	34452.0
27	54166.0
28	79435.0
29	109758.0
30	147867.0
31	195534.0
32	255823.0
33	337071.0
34	454583.0
35	643004.0
36	1076702.0
37	2260948.0
38	2181185.0
39	56833.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.57835608804118	20.07858981050812	14.385744040077093	23.9573100613736
2	14.832353347985999	20.67078348011239	41.582520689233924	22.91434248266769
3	18.207510214630926	26.0407590780734	30.295757356704904	25.455973350590767
4	12.681017671797362	17.91442048347437	37.93592023637108	31.46864160835719
5	13.643788127533002	37.63268280517676	35.05800786732174	13.665521199968497
6	30.485010778115445	37.140359824112466	18.47578858311037	13.89884081466172
7	27.013370136629206	32.45940649637402	22.46789723480577	18.059326132191003
8	24.851765437545623	36.496962112729946	20.758782765315384	17.892489684409046
9	25.828250171570115	16.73755791674628	20.7426947980243	36.6914971136593
10-11	24.611374622603368	26.470765473664127	28.324887427002892	20.592972476729614
12-13	25.48707160106186	23.999742548904663	28.439770641362422	22.073415208671054
14-15	22.623943631399104	25.334843795511368	26.000373051261484	26.04083952182804
16-17	22.204700749426294	28.491803560052542	26.661581225876862	22.6419144646443
18-19	22.291848184385323	27.47906720000485	28.41545253818561	21.813632077424227
20-21	23.089981265077387	26.63607491697381	28.282968636515367	21.990975181433434
22-23	22.797647862579904	26.68764458923641	28.234143200289935	22.280564347893748
24-25	22.617078549192396	26.873931207592694	28.243550826217202	22.265439416997708
26-27	22.526494521337018	26.979857237359226	28.310292711210334	22.183355530093422
28-29	22.362887216889973	26.797536407080074	28.389158533528473	22.450417842501473
30-31	22.62315677993298	26.836849117384766	28.27748682193572	22.262507280746537
32-33	22.26646540884328	26.86546134972994	28.42220024317175	22.445872998255023
34-35	22.411424380848935	26.89003106900387	28.2914829371957	22.40706161295149
36-37	22.5169980241858	26.84118576891226	28.28002203633446	22.36179417056747
38-39	22.396640064057326	26.938062035300693	28.30107968667032	22.36421821397166
40-41	22.588589623521145	26.834530789749422	28.21578478856871	22.361094798160725
42-43	22.595182181371626	26.845497741611073	28.03224526115404	22.527074815863262
44-45	22.555254093130934	27.074174314918313	27.79790610158163	22.572665490369122
46-47	22.811157403416356	26.92921149685642	27.421907007500906	22.83772409222632
48-49	22.677749898357664	27.03866101215179	27.451380031295614	22.832209058194934
50-51	22.56349114928657	27.23980587859333	27.425568822881786	22.77113414923831
52-53	22.766085061926386	27.186694117146605	27.36516178684476	22.68205903408225
54-55	22.77487001673069	27.130833489718736	27.25035201148986	22.84394448206071
56-57	22.862654282398662	27.340443118238237	27.068806210941922	22.728096388421186
58-59	22.647775006143718	27.452532646389	27.29937346169413	22.600318885773152
60-61	22.865846659227618	27.524710441226002	27.036038687060483	22.573404212485894
62-63	22.791087409614487	27.65613528372021	26.96678112352156	22.585996183143738
64-65	22.772643534508383	27.523389233884377	26.97722328203293	22.72674394957431
66-67	22.85473422281389	27.49132656587765	26.835137427748922	22.81880178355954
68-69	22.77411418944808	27.377153718307024	26.994559233759848	22.85417285848505
70-71	22.77244140003071	27.164569802426826	27.09591208644213	22.96707671110033
72-73	22.867219102773696	27.208084472751633	26.947049856219095	22.977646568255576
74-75	22.65178952849577	27.423563764881564	26.973276963004917	22.95136974361775
76-77	22.846029544360114	27.301908428190124	26.91521757747252	22.936844449977237
78-79	22.765492949623933	27.2708391942292	26.887497457361004	23.07617039878586
80-81	22.805135025917007	27.36106183054557	26.88440706769446	22.949396075842966
82-83	22.86558113851198	27.287692052566616	26.97766023507464	22.869066573846762
84-85	22.894101594509948	27.299031854707934	26.88625342134362	22.9206131294385
86-87	22.737155787075604	27.367446564415655	26.982359536299032	22.91303811220971
88-89	22.81990186687252	27.28202543041709	26.933128709048106	22.96494399366229
90-91	22.83849913073168	27.36710693930961	26.924709253940872	22.86968467601784
92-93	22.861413526532345	27.311055814197033	26.953576939919472	22.87395371935115
94-95	22.72610173202985	27.34487059477945	27.02091476083724	22.908112912353456
96-97	22.812790217135387	27.407754224902902	27.050461916709324	22.728993641252387
98-99	23.930674945448057	29.30846770754032	27.23406593624103	19.526791410770592
100	22.858196347776747	30.2771739506506	21.017889428955826	25.846740272616824
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2266.0
1	1961.5
2	2268.5
3	3097.5
4	3976.0
5	6238.0
6	8333.5
7	9895.0
8	11089.0
9	11082.5
10	10229.5
11	9305.0
12	9911.0
13	12838.5
14	20074.0
15	30407.5
16	39187.0
17	44527.0
18	45786.5
19	43184.0
20	38498.5
21	33725.0
22	30760.5
23	31240.5
24	35346.5
25	42795.0
26	53556.5
27	66162.0
28	79413.5
29	94426.5
30	109306.5
31	124147.0
32	142865.5
33	162709.0
34	181130.5
35	198989.0
36	219618.0
37	238370.0
38	251885.5
39	263493.5
40	274026.5
41	282171.0
42	287288.0
43	295141.5
44	304314.5
45	312916.0
46	317845.0
47	320500.0
48	331378.0
49	336595.5
50	332299.5
51	330118.5
52	325586.0
53	314990.5
54	299748.5
55	280401.5
56	259215.5
57	236329.0
58	211887.0
59	186725.0
60	157575.5
61	129016.5
62	103442.5
63	79514.5
64	58650.0
65	42845.5
66	31848.5
67	24183.0
68	18108.0
69	12778.5
70	8906.5
71	6133.0
72	4071.0
73	2665.0
74	1706.0
75	1085.5
76	733.0
77	498.0
78	348.0
79	248.5
80	178.5
81	129.0
82	92.0
83	59.0
84	37.0
85	27.0
86	19.5
87	14.0
88	9.5
89	6.5
90	5.0
91	5.0
92	4.0
93	2.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.001463920419266808
5	0.006562401879471899
6	1.2620003614369035E-5
7	0.0
8	0.0
9	6.814801951759279E-4
10-11	8.77090251198648E-4
12-13	0.0011736603361363201
14-15	0.001060080303606999
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	129.0
20-21	483.0
22-23	1061.0
24-25	2548.0
26-27	3449.0
28-29	6153.0
30-31	13777.0
32-33	17479.0
34-35	24505.0
36-37	35746.0
38-39	42873.0
40-41	45550.0
42-43	55279.0
44-45	66304.0
46-47	64462.0
48-49	59974.0
50-51	54365.0
52-53	53421.0
54-55	61643.0
56-57	65193.0
58-59	67405.0
60-61	66890.0
62-63	66667.0
64-65	70623.0
66-67	73164.0
68-69	69430.0
70-71	69225.0
72-73	66163.0
74-75	68563.0
76-77	67341.0
78-79	73141.0
80-81	74742.0
82-83	72587.0
84-85	73307.0
86-87	78226.0
88-89	84035.0
90-91	88398.0
92-93	93652.0
94-95	112628.0
96-97	275891.0
98-99	479441.0
100-101	5058015.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.78385192579474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.04439914950942	74.60482753163859
2	7.602973778869609	12.740128585690227
3	1.8652043490229309	4.688220149696569
4	0.6625483271375573	2.2204340373830447
5	0.30261534830147563	1.2677139766281815
6	0.1727983669660416	0.8686627674541182
7	0.09546391304189823	0.5598834048191542
8	0.062273878899822846	0.41740363588701046
9	0.04047328839242164	0.30519072014585774
>10	0.14567695123620333	1.908953595232212
>50	0.004227381756400279	0.23588341084297107
>100	0.0013184368744410398	0.1689633930110724
>500	2.6829991806789166E-5	0.01373479157093999
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.7860010843107106E-5	0.0	0.0	1.2620003614369035E-5
3	0.0	3.7860010843107106E-5	0.0	6.310001807184517E-5	1.2620003614369035E-5
4	0.0	6.310001807184517E-5	0.0	6.310001807184517E-5	1.2620003614369035E-5
5	0.0	6.310001807184517E-5	0.0	7.572002168621421E-5	1.2620003614369035E-5
6	0.0	6.310001807184517E-5	0.0	7.572002168621421E-5	2.524000722873807E-5
7	0.0	6.310001807184517E-5	0.0	7.572002168621421E-5	2.524000722873807E-5
8	0.0	6.310001807184517E-5	0.0	8.834002530058324E-5	3.7860010843107106E-5
9	0.0	1.8930005421553554E-4	0.0	1.1358003252932131E-4	8.834002530058324E-5
10-11	0.0	1.8930005421553554E-4	0.0	1.2620003614369034E-4	8.834002530058324E-5
12-13	0.0	1.8930005421553554E-4	0.0	1.5144004337242842E-4	9.465002710776777E-5
14-15	0.0	3.470500993951485E-4	0.0	1.5775004517961294E-4	1.3251003795087488E-4
16-17	0.0	3.912201120454401E-4	0.0	1.6406004698679745E-4	1.5144004337242842E-4
18-19	0.0	4.2277012108136265E-4	0.0	1.7668005060116648E-4	2.145400614442736E-4
20-21	0.0	4.5432013011728525E-4	0.0	1.8930005421553554E-4	2.2716006505864262E-4
22-23	0.0	4.7956013734602336E-4	0.0	2.0823005963708908E-4	2.839500813233033E-4
24-25	0.0	5.174201481891304E-4	0.0	2.460900704801962E-4	3.4074009758796393E-4
26-27	0.0	5.300401518034995E-4	0.0	3.3443009578077945E-4	3.470500993951485E-4
28-29	0.0	5.426601554178686E-4	0.0	7.824402240908801E-4	3.5336010120233296E-4
30-31	0.0	6.310001807184518E-4	0.0	0.0019434805566128314	3.65980104816702E-4
32-33	0.0	6.562401879471898E-4	0.0	0.004410691263221978	3.786001084310711E-4
34-35	0.0	7.761302222836957E-4	0.0	0.007445802132477731	3.912201120454401E-4
36-37	0.0	8.960202566202015E-4	0.0	0.012108893467987089	4.0384011565980913E-4
38-39	0.0	9.086402602345705E-4	0.0	0.020053185743232397	4.0384011565980913E-4
40-41	0.0	9.717402783064157E-4	0.0	0.027890207987755565	4.0384011565980913E-4
42-43	0.0	9.969802855351539E-4	0.0	0.03404876975156766	4.0384011565980913E-4
44-45	0.0	0.0011168703198716596	0.0	0.04054176161116053	4.0384011565980913E-4
46-47	0.0	0.0012241403505937965	0.0	0.047173573510511456	4.101501174669937E-4
48-49	0.0	0.0012241403505937965	0.0	0.053187005232758294	4.669401337316543E-4
50-51	0.0	0.0013503403867374869	0.0	0.05789426658091795	4.732501355388388E-4
52-53	0.0	0.0013629603903518557	0.0	0.06238698786763333	4.984901427675769E-4
54-55	0.0	0.0013818903957734095	0.0	0.06691125916338463	5.174201481891304E-4
56-57	0.0	0.0014134404048093319	0.0	0.07137874044287126	5.23730149996315E-4
58-59	0.0	0.0014323704102308854	0.0	0.07614279180729558	5.36350153610684E-4
60-61	0.0	0.0014765404228811773	0.0	0.0807427831247331	5.426601554178685E-4
62-63	0.0	0.0015396404409530222	0.0	0.08568351453975856	6.057601734897137E-4
64-65	0.0	0.001741560498782927	0.0	0.09031505586623201	6.120701752968982E-4
66-67	0.0	0.0017731105078188495	0.0	0.09457430708608155	6.310001807184518E-4
68-69	0.0	0.0017983505150475877	0.0	0.09967278854628664	6.436201843328208E-4
70-71	0.0	0.001817280520469141	0.0	0.1044179099052894	6.436201843328208E-4
72-73	0.0	0.0018362105258906947	0.0	0.10882860116851137	7.067202024046659E-4
74-75	0.0	0.0018930005421553553	0.0	0.11204670209017548	7.067202024046659E-4
76-77	0.0	0.0020065805746846766	0.0	0.11523325300280365	7.130302042118505E-4
78-79	0.0	0.002057060589142153	0.0	0.11728400359013863	7.19340206019035E-4
80-81	0.0	0.002057060589142153	0.0	0.11807906381784387	7.445802132477731E-4
82-83	0.0	0.002057060589142153	0.0	0.11879209402205573	7.887502258980647E-4
84-85	0.0	0.0020823005963708906	0.0	0.11910128411060777	7.950602277052492E-4
86-87	0.0	0.00209492059998526	0.0	0.11918962413590836	8.076802313196183E-4
88	0.0	0.002246360643357688	0.0	0.11919593413771554	8.076802313196183E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10230	0.0	53.368584	1
GTATCAA	19100	0.0	39.04146	1
CTTATAC	6560	0.0	34.53672	1
TCAACGC	23885	0.0	30.685581	4
ATCAACG	24405	0.0	30.084114	3
TATCAAC	24575	0.0	30.031588	2
CAACGCA	24675	0.0	29.703709	5
AACGCAG	25340	0.0	28.955246	6
TATACAC	8935	0.0	27.454065	3
TTATACA	9220	0.0	25.912254	2
ACGCAGA	29120	0.0	25.19711	7
CGCAGAG	30140	0.0	24.358671	8
GTACATG	28080	0.0	22.85543	1
TACATGG	28575	0.0	21.922985	2
GCAGAGT	34310	0.0	21.286438	9
ACATGGG	29175	0.0	21.063679	3
GTGGTAT	5040	0.0	20.532839	1
TACACAT	12015	0.0	20.274923	5
GAGTACT	18610	0.0	20.010437	12-13
ACATCTC	12045	0.0	19.763533	8
>>END_MODULE