FastQCFastQC Report
Fri 27 May 2016
SRR2139539_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139539_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9805851
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129300.049.759161
GTATCAA248350.039.4854281
CTTATAC79800.034.683071
TCAACGC304700.031.6101994
TATCAAC312350.031.0132272
ATCAACG310250.031.003563
CAACGCA311750.030.8546985
AACGCAG320350.030.066266
TATACAC110500.027.7445263
ACGCAGA371400.025.8877077
CGCAGAG382500.025.14818
TTATACA119600.024.494262
GTGGTAT64200.023.6114181
GTACATG367000.022.8911761
TACATGG367400.022.5765152
TGGTATC64650.022.3932692
GCAGAGT439700.021.7900149
ACATGGG368850.021.7835793
TACACAT150200.020.468165
ACACATC143400.019.8649966
Produced by FastQC (version 0.11.5)