FastQCFastQC Report
Fri 27 May 2016
SRR2139538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21031939
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA246200.050.061611
GTATCAA455150.036.010371
TCAACGC562100.028.3175474
ATCAACG563950.028.2172093
CAACGCA576000.027.6269045
TATCAAC592100.027.1346662
AACGCAG590350.026.9624716
ACGCAGA677900.023.449337
CGCAGAG685400.023.1927368
GTACATG588550.021.170141
TACATGG600500.020.2448312
ACATGGG606700.019.5021463
GCAGAGT806800.019.4324259
AGAGTAC730750.018.67677710-11
GAGTACT483350.018.07284712-13
CAGAGTA795000.017.370710-11
CATGGGG434050.017.1121084
GTGGTAT127750.016.3211081
AGTACTT536700.016.20984312-13
TGGTATC116750.015.8065292
Produced by FastQC (version 0.11.5)