##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139538_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21031939
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42382126536217	31.0	31.0	34.0	30.0	34.0
2	31.585759544091488	31.0	31.0	34.0	30.0	34.0
3	31.63479734322166	31.0	31.0	34.0	30.0	34.0
4	35.43047742768748	37.0	35.0	37.0	33.0	37.0
5	35.24090722210634	37.0	35.0	37.0	32.0	37.0
6	35.23270921430497	37.0	35.0	37.0	32.0	37.0
7	35.143005312063714	37.0	35.0	37.0	32.0	37.0
8	35.0751965379892	37.0	35.0	37.0	32.0	37.0
9	36.61845662447005	39.0	35.0	39.0	32.0	39.0
10-11	36.553235248542705	39.0	35.0	39.0	32.0	39.0
12-13	36.50422571594564	38.0	35.0	39.0	32.0	39.0
14-15	37.58359875901124	39.0	36.0	41.0	32.0	41.0
16-17	37.54827331897454	39.0	36.0	41.0	32.0	41.0
18-19	37.559875197431865	39.0	36.0	41.0	32.0	41.0
20-21	37.52897291495068	39.0	36.0	41.0	32.0	41.0
22-23	37.44595719443916	39.0	36.0	41.0	32.0	41.0
24-25	37.31987967734123	39.0	36.0	41.0	31.0	41.0
26-27	37.07660424448327	39.0	36.0	40.5	31.0	41.0
28-29	36.911525840222396	39.0	36.0	40.0	30.5	41.0
30-31	36.73706139543562	38.5	35.5	40.0	30.0	41.0
32-33	36.504813097666826	38.0	35.0	40.0	30.0	41.0
34-35	36.34059159164416	38.0	35.0	40.0	30.0	41.0
36-37	36.17517085716128	38.0	35.0	40.0	30.0	41.0
38-39	35.99515772250068	38.0	34.0	40.0	29.5	41.0
40-41	35.824043925959224	38.0	34.0	40.0	29.0	41.0
42-43	35.6051278600468	38.0	34.0	40.0	28.0	41.0
44-45	36.0495303456178	38.0	34.5	40.0	29.5	41.0
46-47	36.305644205177344	38.0	35.0	40.0	30.0	41.0
48-49	36.35572423097554	38.0	35.0	40.0	30.0	41.0
50-51	36.27216028460627	38.0	35.0	40.0	29.0	41.0
52-53	36.13157890758939	38.0	34.0	40.0	29.0	41.0
54-55	35.99312899044937	38.0	34.0	40.0	28.5	41.0
56-57	35.86065846699749	38.0	34.0	40.0	28.0	41.0
58-59	35.67267866991439	38.0	34.0	40.0	28.0	41.0
60-61	35.479700452125925	37.5	33.5	40.0	28.0	41.0
62-63	35.24928917868593	37.0	33.0	40.0	28.0	41.0
64-65	34.983415092661645	37.0	33.0	39.5	27.5	41.0
66-67	34.677208743634885	36.0	32.5	39.0	26.5	41.0
68-69	34.401685287318394	36.0	32.0	39.0	26.0	40.0
70-71	34.11211532578017	35.0	32.0	39.0	26.0	40.0
72-73	33.80479605882897	35.0	31.5	38.0	26.0	40.0
74-75	33.47039827336752	35.0	31.0	37.5	26.0	39.5
76-77	31.780687446373165	33.5	29.5	35.5	25.0	38.5
78-79	32.80185438547278	34.0	31.0	36.0	26.0	39.0
80-81	32.87806647639015	34.0	31.0	36.0	26.0	38.5
82-83	32.654031018526965	34.0	31.0	36.0	26.0	37.5
84-85	32.41846904972815	34.0	31.0	35.0	26.0	37.0
86-87	32.215625230220574	34.0	31.0	35.0	26.0	36.5
88-89	32.01017466883181	34.0	31.0	35.0	25.5	36.0
90-91	31.863526393041777	34.0	31.0	35.0	25.0	36.0
92-93	31.83275214398043	34.0	31.0	35.0	25.0	35.5
94-95	31.828547261404292	34.0	31.0	35.0	25.5	35.0
96-97	31.946448237206706	34.0	31.0	35.0	26.0	35.0
98-99	32.172984153494184	34.0	31.0	35.0	26.5	35.0
100	30.230694785192103	32.0	28.0	34.0	23.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	6.0
19	35.0
20	318.0
21	2015.0
22	9668.0
23	32055.0
24	79428.0
25	159781.0
26	273733.0
27	410944.0
28	558292.0
29	704909.0
30	851760.0
31	1006825.0
32	1186843.0
33	1411925.0
34	1719035.0
35	2137241.0
36	2758722.0
37	3464150.0
38	3076419.0
39	1158261.0
40	29573.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.516099775679265	21.814488906610087	15.201584599498885	23.46782671821176
2	14.365882416280568	22.595268049707915	41.00460607011503	22.03424346389649
3	17.30016117868744	28.053293427039076	31.474400099239553	23.17214529503393
4	12.436766926527321	19.62166256583507	39.60508965589578	28.336480851741825
5	12.453844602725407	38.69351275695503	36.43312202455513	12.419520615764434
6	28.28030739343624	39.74769991487708	19.393704023200144	12.578288668486534
7	24.960751360109974	33.373546775691956	24.732032552966228	16.933669311231835
8	23.776287016782543	37.33132121647708	21.895231308725936	16.997160458014445
9	25.29891799324827	16.828971403920484	21.853757753861878	36.01835284896937
10-11	23.481228737097716	27.236111311864015	29.843893115688445	19.43876683534982
12-13	24.48496264321267	24.890284629730207	29.778159588941023	20.846593138116106
14-15	21.690017812488694	26.07047170739382	27.263663107893432	24.975847372224052
16-17	21.21823086077708	29.15994153617782	27.9033925277948	21.7184350752503
18-19	21.302250249656083	28.428773047156437	29.483115032745683	20.7858616704418
20-21	22.08141310818753	27.62042973735314	29.320601677942342	20.977555476516983
22-23	21.848576201054154	27.558971424054807	29.17781399243481	21.414638382456232
24-25	21.671209931671186	27.755832460854542	29.14712965975278	21.425827947721494
26-27	21.635804138842808	27.833541393223864	29.072444802922092	21.45820966501124
28-29	21.621767555917156	27.772619698165762	29.124403090992406	21.481209654924672
30-31	21.829854121317474	27.74586679610087	28.979689190011666	21.444589892569994
32-33	21.67959826198747	27.88782545898804	28.93284718605719	21.4997290929673
34-35	21.770323567475312	27.901804597356456	28.94361501935847	21.384256815809763
36-37	21.786617205731996	27.845598547358165	28.850049391391362	21.51773485551848
38-39	21.67174086254122	27.90537561941156	28.85965290362085	21.56323061442637
40-41	21.87561021336807	27.828131500627958	28.749292079486455	21.54696620651752
42-43	21.752502869679212	27.951411804743074	28.5405955931695	21.75548973240822
44-45	21.802304268320665	28.030266394653353	28.41780110500323	21.749628232022754
46-47	21.90863073748275	28.014784032044286	28.195146389819918	21.88143884065304
48-49	21.81996059279777	28.13598128282398	28.14438468496184	21.89967343941641
50-51	21.861596209963363	28.24369991359193	27.99289463223953	21.901809244205175
52-53	21.98135053758607	28.14661245619195	27.945168532093934	21.926868474128046
54-55	21.943209576891533	28.08167183320972	28.038239329378477	21.93687926052027
56-57	21.872555277732893	28.130331369177718	28.03854973718551	21.958563615903877
58-59	21.845870174879863	28.16312475853658	28.025785632704974	21.965219433878584
60-61	21.9389785061921	28.180636590138104	28.022005206923872	21.85837969674592
62-63	21.843284974701024	28.287483271752055	28.019295467511213	21.849936286035714
64-65	21.801459336520953	28.25485922056948	27.95411395246582	21.98956749044375
66-67	21.892861234405032	28.283188061030422	27.82513062533896	21.998820079225585
68-69	21.82654667274118	28.26364200628597	27.88832035067237	22.021490970300484
70-71	21.843271420784827	28.07236721229999	27.968667489344263	22.11569387757092
72-73	21.89239581691933	28.07938490750618	27.83637846077453	22.19184081479996
74-75	21.831301290175467	28.183483677629685	27.858591291237083	22.126623740957772
76-77	21.930635861880468	28.111482706899427	27.87755458291573	22.080326848304374
78-79	21.871035187911207	28.081624820412067	27.836280880361148	22.21105911131558
80-81	21.898543232433063	28.127405738368516	27.812312788128658	22.161738241069763
82-83	21.9203536326988	28.106748869258684	27.928178279481024	22.04471921856149
84-85	21.90998650114587	28.110937961071603	27.953424321034298	22.02565121674823
86-87	21.83768008057182	28.10361469882503	28.01678604441184	22.041919176191307
88-89	21.89071412161298	28.078262659203535	28.021845006357076	22.00917821282641
90-91	21.87973711845021	28.084670510841086	28.088626296862234	21.946966073846472
92-93	21.924508605533024	27.998451712946732	28.15132085503801	21.925718826482235
94-95	21.692482173412518	28.003759145668845	28.33028033458734	21.9734783463313
96-97	21.69315101930189	28.145342275804875	28.46484474775462	21.696661957138613
98-99	22.731748985071153	30.02394017211151	28.813933136282238	18.430377706535104
100	22.378097443013697	30.42655548121413	22.580194521157697	24.615152554614475
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4082.0
1	3728.0
2	5804.0
3	8602.5
4	11018.0
5	17546.0
6	23605.5
7	28482.0
8	32179.0
9	32021.0
10	28613.5
11	24507.0
12	25194.0
13	32503.0
14	50385.5
15	78748.0
16	103702.0
17	117970.0
18	123576.0
19	121417.0
20	116162.0
21	112107.5
22	110431.0
23	117591.0
24	138205.5
25	169780.5
26	210332.5
27	253732.0
28	301383.5
29	356221.5
30	409757.0
31	460848.0
32	513600.5
33	561659.0
34	605716.5
35	647544.0
36	690598.5
37	731363.5
38	762966.0
39	789395.5
40	810887.5
41	826255.5
42	834385.0
43	838378.5
44	843899.5
45	851217.0
46	851144.0
47	846060.5
48	840646.0
49	827099.5
50	806788.5
51	779265.5
52	742545.5
53	698580.5
54	650923.5
55	602683.0
56	552618.0
57	499298.0
58	443106.0
59	382609.5
60	320543.5
61	262651.0
62	210345.0
63	164614.5
64	124763.5
65	94830.0
66	72933.0
67	56023.5
68	43166.0
69	32414.5
70	23898.5
71	17490.5
72	12552.5
73	8869.5
74	6307.5
75	4389.5
76	3025.5
77	2117.5
78	1447.0
79	1006.0
80	716.5
81	519.5
82	392.5
83	280.0
84	207.5
85	166.5
86	132.5
87	104.0
88	67.5
89	34.5
90	23.0
91	20.0
92	14.5
93	8.5
94	5.0
95	4.0
96	4.0
97	3.5
98	2.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.11725024497265801
3	9.50934671311095E-6
4	1.2362150727044234E-4
5	0.0
6	0.0
7	0.0
8	1.2362150727044234E-4
9	0.0
10-11	1.4264020069666424E-5
12-13	8.558412041799856E-5
14-15	3.1380844153266135E-4
16-17	6.133528629956563E-4
18-19	2.852804013933285E-5
20-21	6.181488600407797E-5
22-23	4.7557693664915725E-6
24-25	0.006058535442896186
26-27	3.570071774150976E-5
28-29	0.0021602572683010945
30-31	0.001993750688630994
32-33	0.02291856843102358
34-35	0.015607939676463614
36-37	0.008096688269224486
38-39	0.004010047854527654
40-41	9.39342562414568E-4
42-43	3.4058449984749657E-4
44-45	0.0010254881496566271
46-47	0.0016759284993037914
48-49	0.0012324502542205322
50-51	5.32521925449162E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	6.0149249640725914E-5
60-61	0.0
62-63	0.0
64-65	2.692413317753235E-6
66-67	0.0
68-69	1.1561049733406321E-4
70-71	5.042461706403587E-4
72-73	5.640752382731367E-6
74-75	5.9962174147342874E-5
76-77	2.9777943275041804E-4
78-79	1.7849761019498756E-4
80-81	2.3732904669609193E-5
82-83	9.033162818333551E-6
84-85	3.057567264951045E-6
86-87	1.3053184641399182E-4
88-89	4.7486716461659647E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	847.0
20-21	2829.0
22-23	5637.0
24-25	11317.0
26-27	13618.0
28-29	22073.0
30-31	43421.0
32-33	54043.0
34-35	75229.0
36-37	101581.0
38-39	121512.0
40-41	136443.0
42-43	159987.0
44-45	190737.0
46-47	172303.0
48-49	164037.0
50-51	146787.0
52-53	136190.0
54-55	146088.0
56-57	164359.0
58-59	177688.0
60-61	184092.0
62-63	183503.0
64-65	191761.0
66-67	208319.0
68-69	214634.0
70-71	220601.0
72-73	216584.0
74-75	222689.0
76-77	200093.0
78-79	227757.0
80-81	246718.0
82-83	251698.0
84-85	259733.0
86-87	284361.0
88-89	324254.0
90-91	366932.0
92-93	426331.0
94-95	534267.0
96-97	1031648.0
98-99	1700205.0
100-101	1.1489033E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.54310844649885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.99657480060694	74.35050499934053
2	7.478542061745419	12.495613009722014
3	1.8817091811862692	4.716115025658511
4	0.759306783628054	2.53739395875203
5	0.3353740126036193	1.400909375254082
6	0.17678763813281428	0.8861633294718056
7	0.1132313991845395	0.6621792143135949
8	0.06403804369494552	0.4279949783286772
9	0.043461827374058265	0.3267842541834565
>10	0.14689965422151371	1.8772210411111094
>50	0.003203586746353566	0.1787839297228883
>100	8.18303973352299E-4	0.10809155345598129
>500	4.2200723408525393E-5	0.022967842752446167
>1k	1.0506178628549212E-5	0.009277487932929963
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.754673356555475E-6
2	0.0	1.90186934262219E-5	0.0	0.0	9.50934671311095E-6
3	0.0	1.90186934262219E-5	0.0	4.754673356555475E-6	1.4264020069666424E-5
4	0.0	5.2301406922110224E-5	0.0	2.852804013933285E-5	1.4264020069666424E-5
5	0.0	5.2301406922110224E-5	0.0	3.3282713495888325E-5	1.4264020069666424E-5
6	0.0	5.2301406922110224E-5	0.0	3.3282713495888325E-5	1.90186934262219E-5
7	4.754673356555475E-6	5.2301406922110224E-5	0.0	3.80373868524438E-5	1.90186934262219E-5
8	4.754673356555475E-6	6.181075363522117E-5	0.0	4.754673356555475E-5	1.90186934262219E-5
9	4.754673356555475E-6	1.1886683391388687E-4	0.0	4.754673356555475E-5	1.90186934262219E-5
10-11	4.754673356555475E-6	1.1886683391388687E-4	0.0	8.558412041799856E-5	1.90186934262219E-5
12-13	4.754673356555475E-6	1.1886683391388687E-4	0.0	1.0222547716594271E-4	1.90186934262219E-5
14-15	7.132010034833212E-6	1.402628640183865E-4	0.0	1.1886683391388686E-4	2.6150703461055112E-5
16-17	9.50934671311095E-6	1.4264020069666426E-4	0.0	1.331308539835533E-4	3.3282713495888325E-5
18-19	9.50934671311095E-6	1.6879090415771936E-4	0.0	1.830549242273858E-4	3.80373868524438E-5
20-21	9.50934671311095E-6	1.9969628097532994E-4	0.0	2.3060165779294054E-4	3.80373868524438E-5
22-23	9.50934671311095E-6	2.35356331149496E-4	0.0	3.4471381835027195E-4	4.516939688727701E-5
24-25	9.50934671311095E-6	2.710163813236621E-4	0.0	8.368225107537636E-4	5.70560802786657E-5
26-27	9.50934671311095E-6	2.852804013933285E-4	0.0	0.0019422840661529116	5.70560802786657E-5
28-29	9.50934671311095E-6	2.852804013933285E-4	0.0	0.007267518225495043	5.70560802786657E-5
30-31	9.50934671311095E-6	3.1380844153266135E-4	0.0	0.009461799979545396	5.70560802786657E-5
32-33	9.50934671311095E-6	3.185631148892168E-4	0.0	0.014670544641651918	6.181075363522117E-5
34-35	1.1886683391388686E-5	4.0890190866377083E-4	0.0	0.020887280055348203	6.656542699177665E-5
36-37	1.4264020069666424E-5	4.968633657600472E-4	0.0	0.031594804454311134	6.656542699177665E-5
38-39	1.4264020069666424E-5	5.016180391166027E-4	0.0	0.0643378625242304	6.894276367005439E-5
40-41	1.4264020069666424E-5	5.895794962128789E-4	0.0	0.09869750953537855	7.369743702660987E-5
42-43	1.4264020069666424E-5	6.06220852960823E-4	0.0	0.1210634930046155	7.60747737048876E-5
44-45	1.6641356747944163E-5	6.846729633439884E-4	0.0	0.15060665590557293	7.60747737048876E-5
46-47	3.0905376817610585E-5	7.51238390335765E-4	0.0	0.18143120327612208	7.60747737048876E-5
48-49	3.3282713495888325E-5	7.559930636923205E-4	0.0	0.2097833205012624	8.558412041799856E-5
50-51	3.3282713495888325E-5	8.225584906840972E-4	0.0	0.23894373219701712	8.558412041799856E-5
52-53	3.3282713495888325E-5	8.320678373972081E-4	0.0	0.3098145159131548	9.50934671311095E-5
54-55	3.566005017416606E-5	8.368225107537636E-4	0.0	0.3968868490917552	1.0460281384422045E-4
56-57	4.279206020899928E-5	8.510865308234301E-4	0.0	0.48194082343049777	1.1648949723560913E-4
58-59	4.754673356555475E-5	8.534638675017078E-4	0.0	0.533788159047057	1.2837618062699782E-4
60-61	4.754673356555475E-5	8.653505508930965E-4	0.0	0.5638162986303831	1.331308539835533E-4
62-63	4.754673356555475E-5	8.796145709627629E-4	0.0	0.5865888066716055	1.331308539835533E-4
64-65	4.754673356555475E-5	9.889720581635387E-4	0.0	0.5943840936396783	1.3550819066183105E-4
66-67	4.9924070243832484E-5	0.001015122761624594	0.0	0.6012593513132574	1.3788552734010878E-4
68-69	5.2301406922110224E-5	0.001048405475120482	0.0	0.607718575068138	1.402628640183865E-4
70-71	5.2301406922110224E-5	0.0010698015052249819	0.0	0.6127965662129393	1.473948740532197E-4
72-73	5.2301406922110224E-5	0.001076933515259815	0.0	0.6168142651992287	1.521495474097752E-4
74-75	5.2301406922110224E-5	0.0010983295453643148	0.0	0.619933330921129	1.521495474097752E-4
76-77	5.2301406922110224E-5	0.001148253615608147	0.0	0.6229287751357591	1.521495474097752E-4
78-79	5.4678743600387964E-5	0.0011696496457126468	0.0	0.6249423793022603	1.521495474097752E-4
80-81	5.70560802786657E-5	0.0011696496457126468	0.0	0.6255866375420736	1.6403623080116388E-4
82-83	5.70560802786657E-5	0.0011696496457126468	0.0	0.626095387591225	1.711682408359971E-4
84-85	5.70560802786657E-5	0.001186291002460591	0.0	0.6263901773393314	1.711682408359971E-4
86-87	5.70560802786657E-5	0.0012171963792782015	0.0	0.6265304402033498	1.711682408359971E-4
88	5.70560802786657E-5	0.0012647431128437563	0.0	0.6265375722133846	1.711682408359971E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	24620	0.0	50.06161	1
GTATCAA	45515	0.0	36.01037	1
TCAACGC	56210	0.0	28.317547	4
ATCAACG	56395	0.0	28.217209	3
CAACGCA	57600	0.0	27.626904	5
TATCAAC	59210	0.0	27.134666	2
AACGCAG	59035	0.0	26.962471	6
ACGCAGA	67790	0.0	23.44933	7
CGCAGAG	68540	0.0	23.192736	8
GTACATG	58855	0.0	21.17014	1
TACATGG	60050	0.0	20.244831	2
ACATGGG	60670	0.0	19.502146	3
GCAGAGT	80680	0.0	19.432425	9
AGAGTAC	73075	0.0	18.676777	10-11
GAGTACT	48335	0.0	18.072847	12-13
CAGAGTA	79500	0.0	17.3707	10-11
CATGGGG	43405	0.0	17.112108	4
GTGGTAT	12775	0.0	16.321108	1
AGTACTT	53670	0.0	16.209843	12-13
TGGTATC	11675	0.0	15.806529	2
>>END_MODULE