FastQCFastQC Report
Fri 27 May 2016
SRR2139537_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139537_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18428363
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA206300.046.1395721
GTATCAA366550.035.574611
TCAACGC453950.028.1210174
ATCAACG456050.027.9821363
CAACGCA462700.027.6632965
AACGCAG474600.026.9967526
TATCAAC485300.026.578422
ACGCAGA552450.023.1459067
CGCAGAG562100.022.7790248
GTACATG532250.020.290181
TACATGG542100.019.5421982
GCAGAGT666550.018.990999
ACATGGG545250.018.9419363
AGAGTAC599600.018.77528610-11
GAGTACT353850.017.94726812-13
CATGGGG387650.017.1283194
CAGAGTA657600.016.73497810-11
AGTACTT403400.016.36931212-13
GTACTTT409600.015.52667314-15
CTTATAC169700.013.8333521
Produced by FastQC (version 0.11.5)