##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139537_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18428363
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.006083882762674	33.0	31.0	34.0	30.0	34.0
2	32.173246750131845	33.0	31.0	34.0	30.0	34.0
3	32.23263884046565	34.0	31.0	34.0	30.0	34.0
4	35.889323050560705	37.0	35.0	37.0	35.0	37.0
5	35.76646921921388	37.0	35.0	37.0	35.0	37.0
6	35.78289037393066	37.0	35.0	37.0	33.0	37.0
7	35.67090717715947	37.0	35.0	37.0	33.0	37.0
8	35.65746040492039	37.0	35.0	37.0	33.0	37.0
9	37.238645939414155	39.0	37.0	39.0	33.0	39.0
10-11	37.15404797485267	39.0	37.0	39.0	33.0	39.0
12-13	37.11731058260574	39.0	37.0	39.0	33.0	39.0
14-15	38.308560722403826	40.0	38.0	41.0	33.0	41.0
16-17	38.31101720755121	40.0	38.0	41.0	33.0	41.0
18-19	38.35291460777064	40.0	38.0	41.0	33.5	41.0
20-21	38.32990797694502	40.0	38.0	41.0	33.5	41.0
22-23	38.245349277778644	40.0	38.0	41.0	33.0	41.0
24-25	38.17282280972135	40.0	37.5	41.0	33.0	41.0
26-27	38.00513081828578	40.0	37.0	41.0	33.0	41.0
28-29	37.90633821705498	40.0	37.0	41.0	33.0	41.0
30-31	37.79243919706979	40.0	37.0	41.0	33.0	41.0
32-33	37.95297877961803	40.0	37.0	41.0	33.0	41.0
34-35	37.934859188388856	40.0	37.0	41.0	33.0	41.0
36-37	37.8582203202821	40.0	37.0	41.0	33.0	41.0
38-39	37.67413146371616	40.0	37.0	41.0	32.0	41.0
40-41	37.69462639369225	40.0	37.0	41.0	32.5	41.0
42-43	37.66647796398931	40.0	37.0	41.0	32.0	41.0
44-45	37.646920340418276	40.0	37.0	41.0	32.5	41.0
46-47	37.67457756164919	40.0	37.0	41.0	32.0	41.0
48-49	37.596586058287	40.0	37.0	41.0	32.0	41.0
50-51	37.51280206958482	40.0	36.0	41.0	32.0	41.0
52-53	37.37569732614217	39.0	36.0	41.0	31.0	41.0
54-55	37.27395401986445	39.0	36.0	41.0	31.0	41.0
56-57	37.130499283470996	39.0	35.5	41.0	31.0	41.0
58-59	36.98558749820995	39.0	35.0	40.0	31.0	41.0
60-61	36.78045563995582	38.5	35.0	40.0	31.0	41.0
62-63	36.54430666203147	38.0	35.0	40.0	31.0	41.0
64-65	36.345690652812394	38.0	35.0	40.0	30.0	41.0
66-67	36.055828341402275	37.5	34.0	40.0	30.0	41.0
68-69	35.77676359848479	37.0	34.0	39.5	30.0	41.0
70-71	35.49180448626393	36.5	34.0	39.0	29.0	41.0
72-73	35.19483500074004	36.0	34.0	39.0	29.0	40.0
74-75	34.86149946167153	35.5	34.0	38.5	29.0	40.0
76-77	33.81194210632226	35.0	32.5	37.0	28.0	39.0
78-79	34.141000080076566	35.0	33.0	37.0	29.0	39.0
80-81	34.033958380168436	35.0	33.0	36.5	29.0	39.0
82-83	33.82219177245979	35.0	33.0	36.0	29.0	38.5
84-85	33.60633087568536	35.0	33.0	36.0	29.0	37.0
86-87	33.40338680960385	35.0	33.0	35.0	29.0	37.0
88-89	33.19004714808946	35.0	33.0	35.0	29.0	36.5
90-91	33.08717432721075	35.0	33.0	35.0	29.0	36.0
92-93	33.015537702597435	34.5	33.0	35.0	29.0	36.0
94-95	32.9702820284158	34.0	33.0	35.0	29.0	35.5
96-97	32.89947915781088	34.0	32.0	35.0	29.0	35.0
98-99	33.0280887917819	34.0	32.5	35.0	29.0	35.0
100-101	32.55984200927726	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	24.0
20	123.0
21	828.0
22	3953.0
23	13575.0
24	35161.0
25	72945.0
26	127844.0
27	197601.0
28	277075.0
29	360448.0
30	450344.0
31	550778.0
32	674461.0
33	846563.0
34	1111832.0
35	1517345.0
36	2284675.0
37	3727727.0
38	4294511.0
39	1816841.0
40	63704.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.52008792099439	22.424281527339136	15.794311193023495	24.26131935864298
2	13.515812744888692	22.799455068817775	41.069936181406405	22.614796004887125
3	16.332308644018593	28.26110856370755	31.906383371273066	23.500199421000794
4	11.84490450942387	19.977368581246203	39.73904790132472	28.438679008005213
5	12.361401823916752	38.728654303152155	36.4557882867838	12.454155586147287
6	28.234911587100818	39.90920951578825	19.343421876376105	12.512457020734832
7	24.948401548200454	33.341577871024136	24.609559731377118	17.10046084939829
8	23.927768299332936	37.33453698518963	21.762741487130462	16.97495322834698
9	25.305877684306523	16.733000104241487	21.61441577854745	36.34670643290454
10-11	23.51689078405933	27.170126288482592	29.82908736929048	19.483895558167593
12-13	24.544372172395345	24.7315537467978	29.693966848818853	21.030107231987998
14-15	21.72567905244758	25.97492517376611	27.08580246655658	25.213593307229733
16-17	21.385030238442774	29.08806387197821	27.636138923462706	21.890766966116306
18-19	21.480836360777133	28.41531013905033	29.14230091951195	20.961552580660584
20-21	22.27467985141704	27.556995122467953	28.914264373101272	21.25406065301373
22-23	21.97268333628806	27.560787632270422	28.80204193441145	21.664487097030065
24-25	21.742675344293907	27.80349073699673	28.80538910932683	21.648444809382532
26-27	21.778291253975247	27.85120096347122	28.695327263408128	21.6751805191454
28-29	21.67798074935116	27.776454699793064	28.767537914877998	21.778026635977778
30-31	21.864441756173804	27.686194545738076	28.732170663835987	21.717193034252137
32-33	21.715261119041674	27.847579255684323	28.70822943485896	21.728930190415042
34-35	21.831171349296515	27.92797179498383	28.745215415537885	21.49564144018177
36-37	21.90829947370847	27.83404162077489	28.55481913916306	21.702839766353577
38-39	21.840566870019046	27.82830288486663	28.624337921422203	21.70679232369212
40-41	21.917127049919895	27.788705718241264	28.5911092804797	21.703057951359142
42-43	21.872860046246924	27.871929349873632	28.4236881838524	21.83152242002705
44-45	21.85815140769855	27.983143818875593	28.266625705760536	21.892079067665318
46-47	22.021651621886928	27.96663574107082	27.956981534067953	22.054731102974294
48-49	21.963032953116905	28.00486618770219	27.99221229238568	22.039888566795224
50-51	21.921896476785193	28.109903364232903	27.899710163044677	22.06848999593723
52-53	22.036274207815776	28.069563182496488	27.86856364296974	22.025598966717997
54-55	22.0278259923057	27.99681706088139	27.87446497479515	22.10089197201776
56-57	22.039878958239484	28.099571325180033	27.779501716846667	22.08104799973382
58-59	21.97280773006087	28.141420720951476	27.911901892777763	21.97386965620989
60-61	22.109686240797295	28.170575920558534	27.776312093776106	21.943425744868065
62-63	22.030021844563485	28.312257119556676	27.724667298011262	21.933053737868573
64-65	22.007639434541428	28.190639350389983	27.750611688048636	22.051109527019953
66-67	22.063079470755923	28.183089792872472	27.655617532788728	22.098213203582876
68-69	22.019851379032442	28.121225147367717	27.71596528215695	22.142958191442887
70-71	22.007376262506245	27.960161677447644	27.77020189636421	22.2622601636819
72-73	22.02698262649195	28.004375375135997	27.706909865355016	22.261732133017034
74-75	21.9389699729137	28.07927019262252	27.735346796234523	22.246413038229257
76-77	22.03995277844649	28.03886032981885	27.678672137924693	22.242514753809967
78-79	21.985197057494478	27.99829039622292	27.648363059968666	22.36814948631394
80-81	22.016901378108994	28.061546319580565	27.618902101914255	22.302650200396183
82-83	22.023004240545593	28.03305347381329	27.724075641655304	22.219866643985814
84-85	22.062658766412305	28.069098030380445	27.650967456060975	22.217275747146275
86-87	21.96455870310566	28.071294292271386	27.72746194675819	22.236685057864765
88-89	22.031030255758697	28.01937266191996	27.71337104152301	22.23622604079833
90-91	21.990468299736065	28.102944346923703	27.72813874623934	22.178448607100893
92-93	22.03586955422052	28.015175107040776	27.744115444355703	22.204839894383007
94-95	21.8101065690254	28.03320165699677	27.88814131921928	22.26855045475855
96-97	21.807966845685826	28.111708826206733	27.889986196215595	22.19033813189185
98-99	21.99816925927654	28.699072541613653	27.998491658793274	21.304266540316537
100-101	22.57466792132255	31.427759177944125	25.16791141575328	20.829661484980043
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4154.0
1	3749.5
2	5185.5
3	7368.5
4	9400.5
5	14038.5
6	17852.0
7	20681.5
8	22334.5
9	21637.5
10	19817.5
11	18114.5
12	20025.5
13	26770.0
14	42788.5
15	65481.5
16	83466.5
17	92228.0
18	93337.5
19	89826.0
20	84315.0
21	80588.5
22	82586.0
23	93078.0
24	112335.0
25	140472.5
26	175641.5
27	213519.0
28	256338.0
29	302549.0
30	347312.5
31	392013.5
32	435738.5
33	477480.5
34	517055.0
35	553636.5
36	594525.5
37	633595.5
38	662196.0
39	685648.5
40	705347.0
41	721099.5
42	728550.0
43	732503.5
44	738617.0
45	746625.5
46	755235.0
47	758476.5
48	750908.5
49	736567.0
50	720066.5
51	693519.0
52	657559.5
53	618062.5
54	575725.5
55	531661.0
56	485534.5
57	436381.0
58	387585.0
59	333572.0
60	275081.0
61	221625.5
62	175277.0
63	135780.5
64	100647.0
65	73714.0
66	55071.0
67	41725.0
68	31725.5
69	23338.0
70	17056.0
71	12428.0
72	8731.5
73	5917.5
74	4079.0
75	2822.5
76	1938.5
77	1266.0
78	824.5
79	571.0
80	417.0
81	306.5
82	230.0
83	157.5
84	104.5
85	72.5
86	58.5
87	48.5
88	32.5
89	24.0
90	18.5
91	10.5
92	5.5
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018883934508995726
3	7.054343351061622E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0023470255548212397
22-23	4.69461655023294E-4
24-25	0.0012268602152656534
26-27	0.01225425274568711
28-29	3.586153340330679E-4
30-31	0.002654235706029689
32-33	0.002795310982978463
34-35	0.0016822228586996157
36-37	0.06387108016524819
38-39	0.028100329031210985
40-41	0.026009220246404105
42-43	0.0034451484387535737
44-45	0.0017791257286229542
46-47	0.003654572112121819
48-49	0.0030768751244797587
50-51	7.899444795883248E-4
52-53	0.001037012666862818
54-55	0.0015858849451591036
56-57	0.012145412499558335
58-59	0.009151954386921075
60-61	0.004856582973654538
62-63	0.0026208547319304942
64-65	0.0012903870506785142
66-67	5.589005185485187E-4
68-69	0.0014193392617002686
70-71	4.41231689436428E-5
72-73	5.605014322244694E-4
74-75	2.2520658119261876E-4
76-77	0.004898355865391101
78-79	0.0012714433270235358
80-81	6.31646752285165E-4
82-83	4.6774834762007284E-4
84-85	0.002843395030011098
86-87	0.0033123671864842734
88-89	0.04213924478358366
90-91	3.684693806727677E-4
92-93	4.96852636568318E-4
94-95	2.235752392988094E-4
96-97	0.018308326406864618
98-99	2.3095589552698322E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	476.0
20-21	1750.0
22-23	3576.0
24-25	6529.0
26-27	8834.0
28-29	14174.0
30-31	31580.0
32-33	39804.0
34-35	56054.0
36-37	93417.0
38-39	105419.0
40-41	112888.0
42-43	128089.0
44-45	151366.0
46-47	152801.0
48-49	144206.0
50-51	133996.0
52-53	123008.0
54-55	131942.0
56-57	141046.0
58-59	149429.0
60-61	159346.0
62-63	149238.0
64-65	154371.0
66-67	166090.0
68-69	162352.0
70-71	166356.0
72-73	159433.0
74-75	159095.0
76-77	159517.0
78-79	176713.0
80-81	182294.0
82-83	176792.0
84-85	174951.0
86-87	183433.0
88-89	201432.0
90-91	221336.0
92-93	237186.0
94-95	302237.0
96-97	404614.0
98-99	1565183.0
100-101	1.143601E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.63537619919697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.46703556374253	70.52937317710918
2	8.09493366076918	13.05476042087331
3	2.2886167848375214	5.536304262635105
4	0.9423423704629054	3.0394452620287757
5	0.4355506622092797	1.7560395750527313
6	0.2472515674221061	1.196233389295358
7	0.14582588499133256	0.8231107567099852
8	0.0983862731273203	0.6346731317166732
9	0.06377174596371346	0.46280328539972904
>10	0.21074126573491186	2.563756036258198
>50	0.004270563520596401	0.22665208943304624
>100	0.0012325878360314726	0.1527266458414004
>500	4.106938257502592E-5	0.02412196764642133
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.426417962355094E-6
2	0.0	1.627925388706528E-5	0.0	5.426417962355094E-6	5.426417962355094E-6
3	0.0	1.627925388706528E-5	0.0	1.0852835924710188E-5	5.426417962355094E-6
4	0.0	4.8837761661195845E-5	0.0	1.0852835924710188E-5	5.426417962355094E-6
5	0.0	4.8837761661195845E-5	0.0	1.0852835924710188E-5	5.426417962355094E-6
6	0.0	4.8837761661195845E-5	0.0	1.0852835924710188E-5	5.426417962355094E-6
7	0.0	4.8837761661195845E-5	0.0	1.0852835924710188E-5	5.426417962355094E-6
8	0.0	5.426417962355094E-5	0.0	1.627925388706528E-5	5.426417962355094E-6
9	0.0	9.224910536003659E-5	0.0	1.627925388706528E-5	1.0852835924710188E-5
10-11	0.0	9.224910536003659E-5	0.0	5.969059758590603E-5	1.0852835924710188E-5
12-13	0.0	9.224910536003659E-5	0.0	7.325664249179377E-5	1.3566044905887733E-5
14-15	2.713208981177547E-6	1.275208221153447E-4	0.0	8.410947841650394E-5	2.9845298792953016E-5
16-17	1.0852835924710186E-5	1.3566044905887734E-4	0.0	1.0310194128474679E-4	4.341134369884075E-5
18-19	1.899246286824283E-5	1.5193970294594263E-4	0.0	1.2480761313416716E-4	5.426417962355094E-5
20-21	2.1705671849420376E-5	1.627925388706528E-4	0.0	1.4651328498358753E-4	5.697738860472848E-5
22-23	2.1705671849420376E-5	1.980642556259609E-4	0.0	1.8178500173889566E-4	7.596985147297132E-5
24-25	4.06981347176632E-5	2.2248313645655883E-4	0.0	2.1705671849420373E-4	9.224910536003659E-5
26-27	5.1550970642373395E-5	2.3333597238126903E-4	0.0	2.7403410709893225E-4	9.767552332239169E-5
28-29	5.426417962355094E-5	2.3333597238126903E-4	0.0	5.290757513296216E-4	1.0310194128474679E-4
30-31	5.697738860472848E-5	2.63181271174222E-4	0.0	0.0014298611330805671	1.1395477720945696E-4
32-33	6.511701554826112E-5	2.6589448015539957E-4	0.0	0.003923300186782733	1.275208221153447E-4
34-35	8.13962694353264E-5	3.011661969107077E-4	0.0	0.006975660290607473	1.4108686702123244E-4
36-37	9.767552332239169E-5	3.364379136660158E-4	0.0	0.011411756974832762	1.4108686702123244E-4
38-39	9.767552332239169E-5	3.5814358551543616E-4	0.0	0.020400618329473975	1.5465291192712016E-4
40-41	9.767552332239169E-5	3.6899642144014636E-4	0.0	0.042640792348186324	1.8992462868242828E-4
42-43	9.767552332239169E-5	3.7713604838367904E-4	0.0	0.051895548182982934	1.8992462868242828E-4
44-45	1.0310194128474679E-4	4.232606010636973E-4	0.0	0.06513872122011054	1.8992462868242828E-4
46-47	1.0852835924710188E-4	4.6124552680018295E-4	0.0	0.08053618218829312	1.8992462868242828E-4
48-49	1.0852835924710188E-4	4.6395873578136053E-4	0.0	0.09421346866240914	2.0620388256949355E-4
50-51	1.0852835924710188E-4	4.91090825593136E-4	0.0	0.10786633625569456	2.0620388256949355E-4
52-53	1.1124156822827941E-4	4.938040345743135E-4	0.0	0.12632701016362657	2.3604918136244655E-4
54-55	1.1395477720945696E-4	4.96517243555491E-4	0.0	0.16281424454250223	2.740341070989322E-4
56-57	1.1395477720945696E-4	4.992304525366686E-4	0.0	0.20015071333248646	2.9302656996717507E-4
58-59	1.1395477720945696E-4	5.019436615178462E-4	0.0	0.23903099803276068	2.9845298792953017E-4
60-61	1.2480761313416716E-4	5.100832884613788E-4	0.0	0.2653599779861076	3.17445450797773E-4
62-63	1.2480761313416716E-4	5.209361243860889E-4	0.0	0.27928687968649196	3.255850777413056E-4
64-65	1.2480761313416716E-4	5.969059758590603E-4	0.0	0.2930455624300433	3.255850777413056E-4
66-67	1.2480761313416716E-4	6.050456028025929E-4	0.0	0.3002464190660885	3.3372470468483824E-4
68-69	1.2480761313416716E-4	6.240380656708358E-4	0.0	0.30669571681434754	3.5000395857190354E-4
70-71	1.2480761313416716E-4	6.267512746520133E-4	0.0	0.31318299948834305	3.6357000347779126E-4
72-73	1.2480761313416716E-4	6.294644836331908E-4	0.0	0.3183923607322039	3.9612851125192186E-4
74-75	1.2480761313416716E-4	6.430305285390786E-4	0.0	0.32319745383786935	3.9612851125192186E-4
76-77	1.2480761313416716E-4	6.728758273320315E-4	0.0	0.32747618440118637	3.9612851125192186E-4
78-79	1.3566044905887734E-4	6.918682902002744E-4	0.0	0.33139134496102557	3.9612851125192186E-4
80-81	1.3566044905887734E-4	6.94581499181452E-4	0.0	0.3337545499836312	4.0155492921427696E-4
82-83	1.3837365804005489E-4	6.94581499181452E-4	0.0	0.3354204602980742	4.124077651389871E-4
84-85	1.4108686702123244E-4	6.94581499181452E-4	0.0	0.33688016672994775	4.124077651389871E-4
86-87	1.4108686702123244E-4	7.081475440873398E-4	0.0	0.33708094419455487	4.178341831013422E-4
88-89	1.4108686702123244E-4	7.325664249179377E-4	0.0	0.3370999366574231	4.232606010636973E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20630	0.0	46.139572	1
GTATCAA	36655	0.0	35.57461	1
TCAACGC	45395	0.0	28.121017	4
ATCAACG	45605	0.0	27.982136	3
CAACGCA	46270	0.0	27.663296	5
AACGCAG	47460	0.0	26.996752	6
TATCAAC	48530	0.0	26.57842	2
ACGCAGA	55245	0.0	23.145906	7
CGCAGAG	56210	0.0	22.779024	8
GTACATG	53225	0.0	20.29018	1
TACATGG	54210	0.0	19.542198	2
GCAGAGT	66655	0.0	18.99099	9
ACATGGG	54525	0.0	18.941936	3
AGAGTAC	59960	0.0	18.775286	10-11
GAGTACT	35385	0.0	17.947268	12-13
CATGGGG	38765	0.0	17.128319	4
CAGAGTA	65760	0.0	16.734978	10-11
AGTACTT	40340	0.0	16.369312	12-13
GTACTTT	40960	0.0	15.526673	14-15
CTTATAC	16970	0.0	13.833352	1
>>END_MODULE