FastQCFastQC Report
Fri 27 May 2016
SRR2139536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14539736
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169350.047.5615231
GTATCAA304050.035.4940951
TCAACGC379450.027.6425154
ATCAACG381250.027.5008243
CAACGCA388100.027.0044465
AACGCAG399950.026.3002856
TATCAAC403100.026.2858832
ACGCAGA460050.022.864477
CGCAGAG470500.022.3475768
GTACATG422200.020.0470871
TACATGG426450.019.5973322
GCAGAGT544900.019.0928179
ACATGGG431250.018.805223
AGAGTAC497900.018.77991110-11
GAGTACT304600.017.70051812-13
CAGAGTA538000.017.03935610-11
CATGGGG305500.016.8598124
AGTACTT346550.016.28992512-13
GTACTTT345700.015.64644714-15
GTGGTAT90550.014.3150911
Produced by FastQC (version 0.11.5)