##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139536_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14539736
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.013561525463736	33.0	31.0	34.0	30.0	34.0
2	32.18099097535196	33.0	31.0	34.0	30.0	34.0
3	32.23947958890037	34.0	31.0	34.0	30.0	34.0
4	35.89482553190787	37.0	35.0	37.0	35.0	37.0
5	35.77231477930548	37.0	35.0	37.0	35.0	37.0
6	35.78903152024218	37.0	35.0	37.0	33.0	37.0
7	35.67813858518477	37.0	35.0	37.0	33.0	37.0
8	35.66511021933273	37.0	35.0	37.0	33.0	37.0
9	37.24537440019544	39.0	37.0	39.0	33.0	39.0
10-11	37.16594964310219	39.0	37.0	39.0	33.0	39.0
12-13	37.12750093949436	39.0	37.0	39.0	33.0	39.0
14-15	38.317622307585225	40.0	38.0	41.0	33.0	41.0
16-17	38.325259413238314	40.0	38.0	41.0	33.0	41.0
18-19	38.36684796065073	40.0	38.0	41.0	33.5	41.0
20-21	38.34338619281391	40.0	38.0	41.0	34.0	41.0
22-23	38.261234070159766	40.0	38.0	41.0	33.0	41.0
24-25	38.18667352097475	40.0	38.0	41.0	33.0	41.0
26-27	38.021137179920636	40.0	37.0	41.0	33.0	41.0
28-29	37.921289888465694	40.0	37.0	41.0	33.0	41.0
30-31	37.8018448449807	40.0	37.0	41.0	33.0	41.0
32-33	37.963682311890196	40.0	37.0	41.0	33.0	41.0
34-35	37.951397778630664	40.0	37.0	41.0	33.0	41.0
36-37	37.872240708855	40.0	37.0	41.0	33.0	41.0
38-39	37.68748602412137	40.0	37.0	41.0	32.0	41.0
40-41	37.71199502002303	40.0	37.0	41.0	33.0	41.0
42-43	37.683523826524244	40.0	37.0	41.0	32.5	41.0
44-45	37.66380710210722	40.0	37.0	41.0	32.5	41.0
46-47	37.69117548986597	40.0	37.0	41.0	32.5	41.0
48-49	37.61520948763233	40.0	37.0	41.0	32.0	41.0
50-51	37.53646660688556	40.0	36.0	41.0	32.0	41.0
52-53	37.399359550347505	39.0	36.0	41.0	31.5	41.0
54-55	37.299975517072966	39.0	36.0	41.0	31.0	41.0
56-57	37.16203965848201	39.0	35.5	41.0	31.0	41.0
58-59	37.020633267437304	39.0	35.0	40.0	31.0	41.0
60-61	36.82514096871873	39.0	35.0	40.0	31.0	41.0
62-63	36.58766543747639	38.0	35.0	40.0	31.0	41.0
64-65	36.38278839148424	38.0	35.0	40.0	30.5	41.0
66-67	36.09635498794129	37.5	34.5	40.0	30.0	41.0
68-69	35.816740322223374	37.0	34.0	39.5	30.0	41.0
70-71	35.528691193808015	36.5	34.0	39.0	29.5	41.0
72-73	35.230694899009386	36.0	34.0	39.0	29.0	40.0
74-75	34.89921306347914	35.5	34.0	38.5	29.0	40.0
76-77	33.85400239781365	35.0	32.5	37.0	28.0	39.0
78-79	34.18263569539399	35.0	33.0	37.0	29.0	39.0
80-81	34.07357364397927	35.0	33.0	36.5	29.0	39.0
82-83	33.859627574554736	35.0	33.0	36.0	29.0	38.5
84-85	33.639380835073695	35.0	33.0	36.0	29.0	37.0
86-87	33.43435731963225	35.0	33.0	35.5	29.0	37.0
88-89	33.21767736053657	35.0	33.0	35.0	29.0	36.5
90-91	33.11431722684423	35.0	33.0	35.0	29.0	36.0
92-93	33.04258511145417	34.5	33.0	35.0	29.0	36.0
94-95	32.994806424365095	34.0	33.0	35.0	29.0	36.0
96-97	32.91644872753085	34.0	32.0	35.0	29.0	35.0
98-99	33.041429739922215	34.0	32.5	35.0	29.0	35.0
100-101	32.57526676311046	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	19.0
20	113.0
21	617.0
22	3147.0
23	10369.0
24	26507.0
25	55356.0
26	98340.0
27	152206.0
28	213465.0
29	280045.0
30	350499.0
31	430446.0
32	531101.0
33	664927.0
34	874269.0
35	1194084.0
36	1794139.0
37	2928734.0
38	3391404.0
39	1484982.0
40	54963.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.658847450875314	22.436906694867087	15.85755064603649	24.04669520822111
2	13.574331555804198	22.844716977667208	41.04856359629591	22.532387870232693
3	16.364197709599676	28.22578742698712	31.79019361364988	23.61982124976332
4	11.8659444710688	19.907472872960007	39.842490950317114	28.38409170565408
5	12.34586377634367	38.75194845353451	36.467264605079485	12.434923165042337
6	28.268353703258438	39.99718426799496	19.2912374750133	12.443224553733302
7	25.05216738460726	33.313149564751384	24.674547048171988	16.96013600246937
8	23.884787179079456	37.45845866802534	21.67352969820085	16.983224454694362
9	25.33127836709002	16.75413501318043	21.722210086895664	36.19237653283388
10-11	23.544777566800388	27.29321220137697	29.797549281500025	19.36446095032262
12-13	24.529895178289344	24.909953660781735	29.69561826982278	20.864532891106137
14-15	21.6826632890721	26.0819144171531	27.218984581288137	25.016437712486667
16-17	21.249275090001635	29.136209900922548	27.80897466088793	21.80554034818789
18-19	21.338870939609908	28.483213175259852	29.363432045808807	20.81448383932143
20-21	22.16094040230097	27.65865492102655	29.155431675900722	21.02497300077176
22-23	21.899686444472373	27.58817446545156	29.023982667670147	21.488156422405925
24-25	21.690946466508272	27.769949468443862	29.017139679916237	21.52196438513163
26-27	21.639639789723596	27.879380884629125	28.913690545850162	21.56728877979712
28-29	21.6472341356897	27.894810751142806	28.931416719387116	21.526538393780378
30-31	21.849567367963616	27.812570173992547	28.826822875999365	21.511039582044475
32-33	21.654496762346863	27.964786647039524	28.823926085201084	21.55679050541253
34-35	21.767869713898897	27.973735364330523	28.8492566949365	21.409138226834088
36-37	21.792147096279923	27.908883949518255	28.75432599241023	21.544642961791595
38-39	21.732186866666368	28.00392386411013	28.75292249990671	21.51096676931679
40-41	21.82852700702473	27.911768301226964	28.665193313149313	21.594511378598998
42-43	21.724012456866237	28.05708668260054	28.461271859125308	21.757629001407913
44-45	21.833969193710516	28.128463598173724	28.301205240626427	21.736361967489334
46-47	21.883541256095885	28.102721514758226	28.109356637905208	21.904380591240677
48-49	21.86540225758845	28.21593608458547	28.03340338256869	21.885258275257392
50-51	21.89910780983006	28.265766584257	27.8972356971509	21.93788990876204
52-53	21.99280219269696	28.199032149167397	27.824666094275003	21.983499563860644
54-55	21.975424909539058	28.14816292909388	27.907312944040935	21.96909921732613
56-57	21.931990451587343	28.248241423923798	27.88240177196316	21.9373663525257
58-59	21.927767961693867	28.235462021244494	27.886766885614893	21.950003131446746
60-61	22.01471955149755	28.232344349818796	27.8661600111623	21.88677608752136
62-63	22.025714054030164	28.297810395558326	27.828384885840414	21.848090664571103
64-65	21.97740861671676	28.215359855553	27.8149480417539	21.992283485976337
66-67	22.031797373852385	28.278021040272076	27.70842081663001	21.98176076924553
68-69	21.969295350724792	28.241460091577085	27.758649748392845	22.030594809305278
70-71	21.958221668731998	28.06620312977699	27.857004579715085	22.11857062177593
72-73	21.998791515335036	28.120247720654156	27.754413180760583	22.12654758325022
74-75	21.866893557690197	28.21747979554828	27.789294066691266	22.126332580070255
76-77	21.954210746166027	28.16044508702682	27.770919198218852	22.114424968588295
78-79	21.905757134905937	28.16715970580706	27.71678308144579	22.210300077841215
80-81	21.943469456387984	28.205065662007094	27.72817163952362	22.123293242081306
82-83	21.982607237882007	28.144678809809836	27.819951151004773	22.052762801303384
84-85	21.96496964995785	28.165850548649452	27.775546114326914	22.093633687065783
86-87	21.878356265595862	28.203571292632994	27.83930669495749	22.078765746813655
88-89	21.939114192559458	28.16246172206088	27.792834590696085	22.10558949468358
90-91	21.933629226088033	28.24832816903894	27.80119098973491	22.01685161513812
92-93	21.987836551271336	28.16722929741572	27.793779160444803	22.051154990868145
94-95	21.73494753212214	28.218989296064578	27.90106889097162	22.144994280841665
96-97	21.78023001671513	28.268455695184286	27.892263010708646	22.059051277391937
98-99	21.933386691231966	28.894149776393892	28.00249404789581	21.169969484478337
100-101	22.505837522998306	31.567740631420953	25.20932015001919	20.71710169556155
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3717.0
1	3326.0
2	4515.0
3	6418.5
4	8150.0
5	11915.5
6	15096.5
7	17744.0
8	19480.0
9	19132.0
10	17576.5
11	15926.5
12	17333.0
13	23108.5
14	37167.5
15	57050.5
16	72543.5
17	79673.0
18	80605.0
19	77995.5
20	73596.5
21	70729.0
22	71815.5
23	79086.0
24	94164.0
25	117504.5
26	146219.0
27	176202.0
28	209656.0
29	246493.5
30	281931.0
31	315107.0
32	349199.5
33	384386.5
34	417214.5
35	447889.5
36	479644.0
37	506437.5
38	528039.0
39	547871.5
40	562438.5
41	573566.5
42	579162.5
43	583385.5
44	587055.5
45	589475.5
46	590238.5
47	584402.5
48	576753.0
49	567471.5
50	553682.5
51	534222.0
52	508369.5
53	478200.0
54	446917.0
55	413772.5
56	378476.0
57	340897.0
58	302472.0
59	261451.5
60	217355.0
61	175944.0
62	139570.5
63	107535.0
64	79475.5
65	59408.5
66	45582.5
67	34904.5
68	26734.5
69	19736.0
70	14345.5
71	10509.5
72	7478.5
73	5175.5
74	3561.0
75	2388.5
76	1619.0
77	1082.5
78	726.5
79	490.0
80	324.0
81	226.5
82	173.0
83	127.0
84	99.5
85	84.5
86	65.5
87	45.5
88	29.5
89	16.5
90	10.0
91	7.5
92	7.0
93	6.0
94	3.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018225915518686171
3	4.814392778520876E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0022766082457581407
22-23	4.264826756202348E-4
24-25	0.0011971957959024736
26-27	0.012214194642501123
28-29	3.1680379679708604E-4
30-31	0.002571615705470716
32-33	0.00277702298093591
34-35	0.0016414002418398946
36-37	0.06244912778724461
38-39	0.026495742901925787
40-41	0.0252831750797766
42-43	0.0035929115044990943
44-45	0.0019361035116666847
46-47	0.004013367217258821
48-49	0.0033334871492614725
50-51	8.295959911572408E-4
52-53	0.001095285591092516
54-55	0.001603775959957817
56-57	0.01278917115300133
58-59	0.009814698790461722
60-61	0.005309624671596266
62-63	0.0029310441697915995
64-65	0.0014047423164107147
66-67	6.106311593971093E-4
68-69	0.0013769603727121315
70-71	4.0221965722277706E-5
72-73	5.244708119999409E-4
74-75	1.8899332459143341E-4
76-77	0.004608775191004667
78-79	0.0012552168639015373
80-81	5.694224560499351E-4
82-83	3.9156840487037086E-4
84-85	0.0029967568818256803
86-87	0.0034003929637004744
88-89	0.0412518941945457
90-91	4.3550365521393374E-4
92-93	5.256910762556408E-4
94-95	2.676446244727577E-4
96-97	0.01699465272127057
98-99	2.4075700019787215E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	328.0
20-21	1320.0
22-23	2760.0
24-25	5509.0
26-27	7225.0
28-29	12018.0
30-31	26886.0
32-33	34108.0
34-35	47844.0
36-37	78876.0
38-39	90851.0
40-41	95418.0
42-43	108738.0
44-45	128739.0
46-47	129142.0
48-49	120474.0
50-51	110950.0
52-53	105401.0
54-55	111436.0
56-57	117687.0
58-59	121104.0
60-61	123229.0
62-63	116634.0
64-65	119802.0
66-67	126928.0
68-69	131510.0
70-71	134502.0
72-73	128656.0
74-75	128001.0
76-77	128575.0
78-79	142090.0
80-81	147189.0
82-83	141979.0
84-85	140594.0
86-87	147811.0
88-89	160774.0
90-91	175469.0
92-93	188458.0
94-95	238016.0
96-97	317114.0
98-99	1212823.0
100-101	8932768.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.30119298709904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.22376268952536	72.60920919158656
2	7.870023225843953	12.9542460064627
3	2.083076536830682	5.1431905219379965
4	0.8188474082732508	2.6956847430113084
5	0.3909522807149472	1.6087919551933696
6	0.20206300792820103	0.9978015966631562
7	0.12200613553838408	0.7028875354588299
8	0.0711574388141837	0.4685073683451059
9	0.05109692282700766	0.37848039359692176
>10	0.16189322356008767	2.064372221259906
>50	0.003960792326287315	0.2186271849275495
>100	0.0011446157580269622	0.15060582869738173
>500	1.5722059694003443E-5	0.007595452859211199
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.877703969315536E-6	0.0
2	0.0	2.0633111907946608E-5	0.0	6.877703969315536E-6	0.0
3	0.0	2.0633111907946608E-5	0.0	6.877703969315536E-6	0.0
4	0.0	4.814392778520876E-5	0.0	2.0633111907946608E-5	6.877703969315536E-6
5	0.0	5.502163175452429E-5	0.0	2.0633111907946608E-5	6.877703969315536E-6
6	0.0	5.502163175452429E-5	0.0	2.0633111907946608E-5	1.3755407938631073E-5
7	0.0	5.502163175452429E-5	0.0	2.7510815877262146E-5	1.3755407938631073E-5
8	0.0	6.877703969315536E-5	0.0	2.7510815877262146E-5	2.0633111907946608E-5
9	0.0	1.2379867144767965E-4	0.0	3.438851984657768E-5	2.0633111907946608E-5
10-11	6.877703969315536E-6	1.2379867144767965E-4	0.0	5.1582779769866524E-5	2.0633111907946608E-5
12-13	6.877703969315536E-6	1.2379867144767965E-4	0.0	6.189933572383983E-5	2.0633111907946608E-5
14-15	6.877703969315536E-6	1.513094873249418E-4	0.0	6.189933572383983E-5	3.094966786191991E-5
16-17	6.877703969315536E-6	1.513094873249418E-4	0.0	6.189933572383983E-5	4.814392778520876E-5
18-19	6.877703969315536E-6	1.6850374724823065E-4	0.0	6.877703969315536E-5	5.502163175452429E-5
20-21	6.877703969315536E-6	1.856980071715195E-4	0.0	7.56547436624709E-5	5.846048373918206E-5
22-23	6.877703969315536E-6	2.1320882304878163E-4	0.0	8.941015160110198E-5	7.565474366247091E-5
24-25	6.877703969315536E-6	2.37280786941386E-4	0.0	1.1692096747836412E-4	8.941015160110198E-5
26-27	6.877703969315536E-6	2.475973428953593E-4	0.0	1.2035981946302188E-4	8.941015160110198E-5
28-29	6.877703969315536E-6	2.5103619488001707E-4	0.0	2.2008652701809717E-4	8.941015160110198E-5
30-31	6.877703969315536E-6	2.785470107572792E-4	0.0	7.393531767014201E-4	9.284900358575975E-5
32-33	6.877703969315536E-6	2.81985862741937E-4	0.0	0.0018707354796538259	9.628785557041751E-5
34-35	6.877703969315536E-6	3.163743825885147E-4	0.0	0.003218765457639671	1.1692096747836412E-4
36-37	6.877703969315536E-6	3.507629024350924E-4	0.0	0.0054230695798053	1.1692096747836412E-4
38-39	6.877703969315536E-6	3.576406064044079E-4	0.0	0.009281461506591315	1.2035981946302188E-4
40-41	6.877703969315536E-6	3.782737183123545E-4	0.0	0.013968616761679854	1.2379867144767965E-4
42-43	6.877703969315536E-6	3.885902742663278E-4	0.0	0.01801270669563739	1.2723752343233742E-4
44-45	1.3755407938631073E-5	4.2985649808222103E-4	0.0	0.022878682253928133	1.306763754169952E-4
46-47	1.3755407938631073E-5	4.6080616594414093E-4	0.0	0.028281118721825486	1.306763754169952E-4
48-49	1.3755407938631073E-5	4.6768386991345647E-4	0.0	0.033686994041707496	1.3755407938631072E-4
50-51	1.3755407938631073E-5	5.158277976986652E-4	0.0	0.03929576162868431	1.3755407938631072E-4
52-53	1.3755407938631073E-5	5.158277976986652E-4	0.0	0.04477041398825948	1.650648952635729E-4
54-55	1.3755407938631073E-5	5.158277976986652E-4	0.0	0.050609584658208376	2.0289226709480833E-4
56-57	1.3755407938631073E-5	5.227055016679807E-4	0.0	0.056741057746853174	2.1320882304878163E-4
58-59	1.3755407938631073E-5	5.227055016679807E-4	0.0	0.06253208448901686	2.166476750334394E-4
60-61	1.3755407938631073E-5	5.364609096066118E-4	0.0	0.06850880923835206	2.3384193495672823E-4
62-63	1.3755407938631073E-5	5.433386135759274E-4	0.0	0.07474344788653658	2.475973428953593E-4
64-65	1.3755407938631073E-5	5.983602453304516E-4	0.0	0.08124631698952443	2.475973428953593E-4
66-67	1.3755407938631073E-5	6.017990973151094E-4	0.0	0.08774230838854295	2.5103619488001707E-4
68-69	1.3755407938631073E-5	6.086768012844249E-4	0.0	0.09464408432175109	2.5447504686467484E-4
70-71	1.3755407938631073E-5	6.121156532690827E-4	0.0	0.10096125541756742	2.613527508339904E-4
72-73	1.3755407938631073E-5	6.155545052537405E-4	0.0	0.10647717400095848	2.682304548033059E-4
74-75	1.3755407938631073E-5	6.568207290696337E-4	0.0	0.11120559547986292	2.682304548033059E-4
76-77	1.3755407938631073E-5	6.808926929622382E-4	0.0	0.11539755604916072	2.7510815877262144E-4
78-79	1.3755407938631073E-5	6.877703969315537E-4	0.0	0.11951386187479608	2.7510815877262144E-4
80-81	1.3755407938631073E-5	6.877703969315537E-4	0.0	0.12205517349145817	2.785470107572792E-4
82-83	1.3755407938631073E-5	6.877703969315537E-4	0.0	0.12391559241515801	2.923024186959103E-4
84-85	1.3755407938631073E-5	6.98086952885527E-4	0.0	0.12557311907176305	2.9574127068056805E-4
86-87	1.3755407938631073E-5	7.42792028686078E-4	0.0	0.12575881707893458	2.9574127068056805E-4
88-89	1.3755407938631073E-5	8.0125251242526E-4	0.0	0.12578632789481184	2.9574127068056805E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16935	0.0	47.561523	1
GTATCAA	30405	0.0	35.494095	1
TCAACGC	37945	0.0	27.642515	4
ATCAACG	38125	0.0	27.500824	3
CAACGCA	38810	0.0	27.004446	5
AACGCAG	39995	0.0	26.300285	6
TATCAAC	40310	0.0	26.285883	2
ACGCAGA	46005	0.0	22.86447	7
CGCAGAG	47050	0.0	22.347576	8
GTACATG	42220	0.0	20.047087	1
TACATGG	42645	0.0	19.597332	2
GCAGAGT	54490	0.0	19.092817	9
ACATGGG	43125	0.0	18.80522	3
AGAGTAC	49790	0.0	18.779911	10-11
GAGTACT	30460	0.0	17.700518	12-13
CAGAGTA	53800	0.0	17.039356	10-11
CATGGGG	30550	0.0	16.859812	4
AGTACTT	34655	0.0	16.289925	12-13
GTACTTT	34570	0.0	15.646447	14-15
GTGGTAT	9055	0.0	14.315091	1
>>END_MODULE