FastQCFastQC Report
Fri 27 May 2016
SRR2139535_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139535_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21692965
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA265050.049.590841
GTATCAA483050.036.2111471
TCAACGC597300.028.5836734
ATCAACG600200.028.480523
CAACGCA607100.028.0738895
TATCAAC625450.027.5637592
AACGCAG623300.027.4519376
ACGCAGA714000.023.923567
CGCAGAG732700.023.3015278
GTACATG633800.020.07571
GCAGAGT850000.019.8491559
TACATGG646050.019.2008722
ACATGGG645900.018.7609483
AGAGTAC772250.018.6204910-11
GAGTACT491800.018.47585512-13
CAGAGTA831100.017.83209210-11
CATGGGG463950.016.9028914
GTGGTAT128000.016.4746971
AGTACTT552500.016.24476212-13
GTACTTT562650.016.16887914-15
Produced by FastQC (version 0.11.5)