FastQCFastQC Report
Fri 27 May 2016
SRR2139534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23072833
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA281450.050.2686271
GTATCAA511150.036.2752341
TCAACGC643600.028.1374074
ATCAACG646350.027.9982153
CAACGCA658550.027.4795345
TATCAAC676200.026.9737822
AACGCAG674000.026.8994876
ACGCAGA770850.023.4926057
CGCAGAG790450.022.8835418
GTACATG667900.020.4030761
TACATGG672950.019.8317742
GCAGAGT908250.019.638499
ACATGGG677900.019.2368553
AGAGTAC824950.018.7760110-11
GAGTACT546600.018.20459712-13
CAGAGTA895450.017.64450310-11
CATGGGG493100.017.0182024
GTACTTT625450.015.91043914-15
AGTACTT614650.015.80340612-13
GTGGTAT133150.014.76491451
Produced by FastQC (version 0.11.5)