FastQCFastQC Report
Fri 27 May 2016
SRR2139533_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139533_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22855102
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA281550.049.935911
GTATCAA515250.036.330481
TCAACGC647950.028.392564
ATCAACG652300.028.2161063
CAACGCA660100.027.8317435
TATCAAC677400.027.323372
AACGCAG673850.027.3138056
ACGCAGA773500.023.746057
CGCAGAG790850.023.2569948
GCAGAGT912650.019.8953029
GTACATG646800.019.836591
TACATGG653500.019.1823622
AGAGTAC833700.018.81458310-11
ACATGGG654750.018.4856573
GAGTACT553200.018.3380812-13
CAGAGTA896600.017.6939510-11
CATGGGG467000.016.69024
AGTACTT616650.016.56709112-13
GTACTTT634450.015.98043214-15
GTGGTAT140850.015.3015391
Produced by FastQC (version 0.11.5)