##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139533_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22855102
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.456174993224707	31.0	31.0	34.0	30.0	34.0
2	31.62555244776418	31.0	31.0	34.0	30.0	34.0
3	31.674045165057674	31.0	31.0	34.0	30.0	34.0
4	35.464453101106265	37.0	35.0	37.0	33.0	37.0
5	35.28376478039783	37.0	35.0	37.0	33.0	37.0
6	35.27281672162303	37.0	35.0	37.0	32.0	37.0
7	35.18277791978351	37.0	35.0	37.0	32.0	37.0
8	35.11626988144704	37.0	35.0	37.0	32.0	37.0
9	36.66393223709962	39.0	35.0	39.0	32.0	39.0
10-11	36.60227189097647	39.0	35.0	39.0	32.0	39.0
12-13	36.55211460005735	39.0	35.0	39.0	32.0	39.0
14-15	37.639227315633946	39.0	36.0	41.0	32.0	41.0
16-17	37.60903655122607	39.0	36.0	41.0	32.0	41.0
18-19	37.62484433453852	39.0	36.0	41.0	32.0	41.0
20-21	37.594653168353105	39.0	36.0	41.0	32.0	41.0
22-23	37.51400697179402	39.0	36.0	41.0	32.0	41.0
24-25	37.39177991346611	39.0	36.0	41.0	31.5	41.0
26-27	37.15307527471316	39.0	36.0	41.0	31.0	41.0
28-29	36.991639444490225	39.0	36.0	40.0	31.0	41.0
30-31	36.81699567570317	39.0	35.5	40.0	30.0	41.0
32-33	36.58744274795553	38.0	35.0	40.0	30.0	41.0
34-35	36.42913484080826	38.0	35.0	40.0	30.0	41.0
36-37	36.26524364676064	38.0	35.0	40.0	30.0	41.0
38-39	36.09326045322993	38.0	34.5	40.0	29.5	41.0
40-41	35.92839939717547	38.0	34.0	40.0	29.0	41.0
42-43	35.714353395534275	38.0	34.0	40.0	28.5	41.0
44-45	36.151706834536824	38.0	34.5	40.0	29.5	41.0
46-47	36.39593197658552	38.0	35.0	40.0	30.0	41.0
48-49	36.44383360652018	38.0	35.0	40.0	30.0	41.0
50-51	36.368397591825655	38.0	35.0	40.0	29.5	41.0
52-53	36.23315272730716	38.0	34.5	40.0	29.0	41.0
54-55	36.098970081632345	38.0	34.0	40.0	29.0	41.0
56-57	35.97088386394841	38.0	34.0	40.0	28.0	41.0
58-59	35.78735098336773	38.0	34.0	40.0	28.0	41.0
60-61	35.598250737510554	38.0	34.0	40.0	28.0	41.0
62-63	35.37024847727383	37.0	33.5	40.0	28.0	41.0
64-65	35.106182849814644	37.0	33.0	40.0	28.0	41.0
66-67	34.800149908962254	36.0	33.0	39.0	27.5	41.0
68-69	34.527978107275125	36.0	32.0	39.0	26.5	40.5
70-71	34.24129557207832	35.5	32.0	39.0	26.0	40.0
72-73	33.932767972633485	35.0	32.0	38.0	26.0	40.0
74-75	33.600729025537206	35.0	31.5	37.5	26.0	39.5
76-77	31.917591678259882	33.5	29.5	35.5	25.0	38.5
78-79	32.926680773501616	34.0	31.0	36.0	26.0	39.0
80-81	32.99826973393217	34.0	31.0	36.0	26.0	39.0
82-83	32.77117820097928	34.0	31.0	36.0	26.0	37.5
84-85	32.53387809348654	34.0	31.0	35.0	26.0	37.0
86-87	32.327992433769296	34.0	31.0	35.0	26.0	37.0
88-89	32.12055969764747	34.0	31.0	35.0	26.0	36.0
90-91	31.971004279017105	34.0	31.0	35.0	26.0	36.0
92-93	31.935676526538913	34.0	31.0	35.0	26.0	36.0
94-95	31.928027636471597	34.0	31.0	35.0	26.0	35.0
96-97	32.03786662895698	34.0	31.0	35.0	26.0	35.0
98-99	32.25117973917844	34.0	31.0	35.0	27.0	35.0
100	30.334838118785022	32.0	29.0	34.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	6.0
19	33.0
20	312.0
21	2143.0
22	9793.0
23	32226.0
24	81141.0
25	165594.0
26	283346.0
27	428026.0
28	585269.0
29	743741.0
30	904353.0
31	1072042.0
32	1267400.0
33	1511262.0
34	1845134.0
35	2295030.0
36	2983878.0
37	3794833.0
38	3461436.0
39	1351038.0
40	37064.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.63880362467864	21.75687074159634	15.274672587328642	23.32965304639638
2	14.28767430531949	22.504748604330867	41.26714141028292	21.940435680066724
3	17.202553600616337	28.146657501097895	31.519961342546683	23.13082755573909
4	12.271282218826041	19.666019939464654	39.96164615853186	28.10105168317744
5	12.374226113714128	38.814383764290355	36.407389474787735	12.404000647207788
6	28.04594790257335	39.917196606692016	19.527416679216746	12.509438811517883
7	24.87687431891575	33.51130526566891	24.78419479379265	16.82762562162269
8	23.779385872011872	37.37501079082093	21.845311979532948	17.000291357634254
9	25.299747076167062	16.89848069809533	21.90856116065463	35.89321106508297
10-11	23.435774660175397	27.307637127277374	29.89401839825765	19.362569814289582
12-13	24.50830781511495	24.93474261615357	29.836878909593672	20.720070659137807
14-15	21.662679541764636	26.122887163078172	27.42279394287212	24.791639352285067
16-17	21.111795305118427	29.17606023569464	28.000516386961806	21.711628072225132
18-19	21.17740599542811	28.431688885523727	29.693505805717706	20.697399313330457
20-21	22.004958996748634	27.615666659643708	29.5302390788118	20.84913526479586
22-23	21.735249809222	27.536272984115396	29.425107561942294	21.303369644720316
24-25	21.532487633928877	27.724895752070495	29.40072142958107	21.341895184419567
26-27	21.526770659925816	27.835190430910068	29.264370604417888	21.373668304746225
28-29	21.52716223542663	27.824835791116403	29.294054250689793	21.35394772276717
30-31	21.738887189597385	27.734588996597825	29.192127506328152	21.334396307476634
32-33	21.56071246723944	27.940049202084854	29.099955009818128	21.399283320857574
34-35	21.69324384721535	27.924139748731353	29.128667684117936	21.253948719935366
36-37	21.70809708666311	27.877434837729453	29.031413897788372	21.38305417781907
38-39	21.606920456912466	28.001158556249784	29.024727998956934	21.367192987880816
40-41	21.7994767304826	27.866194019563178	28.903902730057613	21.43042651989661
42-43	21.654490405293096	28.029566321314086	28.706281833922652	21.60966143947016
44-45	21.745472649557133	28.145014366398	28.499574966851238	21.609938017193628
46-47	21.848663391777944	28.152343765700948	28.226694266839313	21.7722985756818
48-49	21.781435246032817	28.236618778056943	28.19310193107862	21.788844044831617
50-51	21.81986758233988	28.342205605272635	28.062342338655483	21.775584473731996
52-53	21.957615673919353	28.259044515382897	27.973076134494097	21.810263676203654
54-55	21.903276326451543	28.19417382864458	28.065388463376234	21.837161381527643
56-57	21.831546446361944	28.28310502042779	28.047919334668624	21.837429198541642
58-59	21.85228813365604	28.2945013696049	28.069303038731597	21.78390745800746
60-61	21.915982298104584	28.31745029392007	28.014016311297453	21.752551096677895
62-63	21.881225147226473	28.399105196219924	27.988442736929414	21.731226919624184
64-65	21.861242394952694	28.290273091444142	28.00278070397266	21.8457038096305
66-67	21.908060307587853	28.31070723822559	27.92403297630155	21.857199477885008
68-69	21.81411150895037	28.276325486955763	28.01861847147553	21.890944532618338
70-71	21.831118685634753	28.112658588275067	28.074287421104305	21.98193530498587
72-73	21.92065602210927	28.17567122763094	27.96380994468529	21.9398628055745
74-75	21.762447149721666	28.261889418103074	28.00966906417977	21.965994367995485
76-77	21.829455117726166	28.226884922182705	28.023965095461516	21.919694864629616
78-79	21.775370491704553	28.153584038926194	28.027331467644807	22.04371400172445
80-81	21.78438722063363	28.209776607692323	28.04274632778136	21.963089843892693
82-83	21.860231193090677	28.15134056371034	28.121278311084964	21.867149932114017
84-85	21.860280160181578	28.163326260473227	28.094416485676632	21.88197709366856
86-87	21.726438815307816	28.15042037958822	28.227927002852997	21.895213802250968
88-89	21.79566808826685	28.118829801595048	28.18554965205353	21.899952458084574
90-91	21.79399765096036	28.128459539668242	28.235128617791506	21.84241419157989
92-93	21.82425934271049	28.037917842311355	28.286196788185837	21.85162602679232
94-95	21.556724006425597	28.017195845787562	28.479591122247726	21.94648902553911
96-97	21.60598077083365	28.195617734103518	28.55188224990513	21.646519245157698
98-99	22.575667981794485	30.161277599394527	28.90660558496253	18.356448833848457
100	22.26462889617067	30.588543684175328	22.684804319271812	24.462023100382186
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4346.0
1	4157.0
2	6840.5
3	10199.5
4	13131.0
5	21072.0
6	28450.0
7	34286.0
8	38502.0
9	37738.5
10	33194.5
11	28191.0
12	29151.0
13	38157.5
14	59950.5
15	94356.5
16	124239.5
17	140979.0
18	147391.5
19	143890.0
20	136435.0
21	131098.0
22	128817.5
23	136989.0
24	159419.0
25	194355.5
26	238073.5
27	284691.5
28	335904.0
29	392132.5
30	446759.5
31	499995.0
32	558056.5
33	613856.0
34	663726.0
35	711756.5
36	761127.0
37	806511.0
38	838929.5
39	862204.0
40	883192.0
41	896636.0
42	901669.5
43	906840.5
44	913036.0
45	919663.5
46	919361.0
47	910902.5
48	897812.5
49	878927.5
50	857981.5
51	828311.5
52	790450.5
53	746373.5
54	696947.0
55	645460.5
56	592911.5
57	535984.5
58	474623.5
59	412212.0
60	345152.0
61	280770.5
62	224011.0
63	173645.0
64	130344.0
65	97652.5
66	74038.0
67	56674.5
68	44094.0
69	33683.5
70	25139.0
71	18498.0
72	13409.0
73	9515.0
74	6638.5
75	4682.0
76	3253.5
77	2193.0
78	1551.0
79	1109.0
80	796.5
81	580.5
82	409.5
83	270.0
84	188.0
85	136.5
86	102.5
87	77.5
88	52.5
89	30.5
90	19.5
91	14.5
92	12.0
93	10.0
94	6.5
95	4.0
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.11963193163609595
3	4.3753906676942415E-6
4	1.0938476669235604E-4
5	0.0
6	0.0
7	0.0
8	1.26886329363133E-4
9	0.0
10-11	1.3126172003082725E-5
12-13	1.1157246202620317E-4
14-15	3.43468167413998E-4
16-17	6.497455141525949E-4
18-19	1.5313867336929847E-5
20-21	7.87620961350455E-5
22-23	4.376394811731434E-6
24-25	0.0066587449053870495
26-27	3.2852691880582045E-5
28-29	0.002334372900091843
30-31	0.0021113885180058644
32-33	0.024382344727685832
34-35	0.01636237566783716
36-37	0.008620444359216774
38-39	0.004267269150756007
40-41	9.20656996064673E-4
42-43	3.338196122721292E-4
44-45	0.0010488636927005026
46-47	0.0018305355003076311
48-49	0.0013598829750041694
50-51	6.306800181159332E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	4.586226981062986E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	1.1666330963766253E-4
70-71	4.6947186108804804E-4
72-73	7.787585607306167E-6
74-75	6.041454093945399E-5
76-77	3.534944601836598E-4
78-79	1.6131395594247007E-4
80-81	2.179200150190474E-5
82-83	8.289223382384997E-6
84-85	0.0
86-87	1.2246566909930004E-4
88-89	5.536525560631187E-4
90-91	2.959084414304131E-6
92-93	3.0303048668514347E-6
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	888.0
20-21	3037.0
22-23	6314.0
24-25	11907.0
26-27	16328.0
28-29	24518.0
30-31	46754.0
32-33	59087.0
34-35	81504.0
36-37	109077.0
38-39	138738.0
40-41	148214.0
42-43	177231.0
44-45	212749.0
46-47	190564.0
48-49	179722.0
50-51	167914.0
52-53	161270.0
54-55	173260.0
56-57	184870.0
58-59	191597.0
60-61	196519.0
62-63	191130.0
64-65	197798.0
66-67	214306.0
68-69	222092.0
70-71	230052.0
72-73	226023.0
74-75	229531.0
76-77	207197.0
78-79	236111.0
80-81	257484.0
82-83	262348.0
84-85	271399.0
86-87	296282.0
88-89	338820.0
90-91	385791.0
92-93	446943.0
94-95	556773.0
96-97	1091758.0
98-99	1830546.0
100-101	1.2680656E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.64094571617869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.03453093773538	70.99187830499173
2	7.635338461820987	12.314418288487152
3	2.1586842039238676	5.2223500712099105
4	0.8961103710983532	2.890527511657882
5	0.4422207689964455	1.783055051360458
6	0.24502525940235934	1.1855441185534956
7	0.15708772467037602	0.8867391874475272
8	0.1053276168071175	0.6794974903485785
9	0.07573721497481264	0.549676857733058
>10	0.2438985538340788	3.0254346352653028
>50	0.0047514144283902875	0.2525941352220694
>100	0.0012052486936047768	0.1611529720992474
>500	4.7035830224453895E-5	0.02441414900931229
>1k	3.5187783906406354E-5	0.032717226614189986
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.3753906676942415E-6	0.0
2	8.750781335388483E-6	1.7501562670776966E-5	0.0	1.7501562670776966E-5	0.0
3	8.750781335388483E-6	1.7501562670776966E-5	0.0	1.7501562670776966E-5	0.0
4	8.750781335388483E-6	7.438164135080212E-5	0.0	2.625234400616545E-5	0.0
5	8.750781335388483E-6	7.875703201849635E-5	0.0	2.625234400616545E-5	0.0
6	8.750781335388483E-6	8.313242268619059E-5	0.0	2.625234400616545E-5	0.0
7	8.750781335388483E-6	8.313242268619059E-5	0.0	3.0627734673859695E-5	0.0
8	8.750781335388483E-6	1.0063398535696757E-4	0.0	3.0627734673859695E-5	0.0
9	8.750781335388483E-6	1.6188945470468696E-4	0.0	7.875703201849635E-5	0.0
10-11	8.750781335388483E-6	1.6188945470468696E-4	0.0	8.094472735234348E-5	0.0
12-13	8.750781335388483E-6	1.6626484537238118E-4	0.0	8.313242268619059E-5	0.0
14-15	8.750781335388483E-6	2.2314492405240633E-4	0.0	8.969550868773195E-5	0.0
16-17	8.750781335388483E-6	2.3627109605548906E-4	0.0	1.0063398535696757E-4	0.0
18-19	8.750781335388483E-6	2.581480493939603E-4	0.0	1.050093760246618E-4	0.0
20-21	8.750781335388483E-6	3.215912140755268E-4	0.0	1.1157246202620317E-4	0.0
22-23	8.750781335388483E-6	3.762835974217048E-4	0.0	1.1376015736005029E-4	0.0
24-25	8.750781335388483E-6	4.2660059010018856E-4	0.0	1.3126172003082725E-4	0.0
26-27	8.750781335388483E-6	4.331636761017299E-4	0.0	1.7501562670776965E-4	0.0
28-29	8.750781335388483E-6	4.331636761017299E-4	0.0	4.178498087648001E-4	0.0
30-31	8.750781335388483E-6	4.856683641140608E-4	0.0	9.997767675681342E-4	0.0
32-33	8.750781335388483E-6	4.900437547817551E-4	0.0	0.002297080100539477	0.0
34-35	8.750781335388483E-6	5.578623101310158E-4	0.0	0.004007857851607925	2.1876953338471208E-6
36-37	8.750781335388483E-6	6.322439514818179E-4	0.0	0.00679279401159531	4.3753906676942415E-6
38-39	8.750781335388483E-6	6.606839908218305E-4	0.0	0.011662603824739	4.3753906676942415E-6
40-41	8.750781335388483E-6	6.913117254956902E-4	0.0	0.017258728488719937	4.3753906676942415E-6
42-43	8.750781335388483E-6	7.153763741680085E-4	0.0	0.02280016076935469	4.3753906676942415E-6
44-45	8.750781335388483E-6	8.050718828557404E-4	0.0	0.028820698328101968	4.3753906676942415E-6
46-47	8.750781335388483E-6	8.750781335388483E-4	0.0	0.03549316909633569	4.3753906676942415E-6
48-49	8.750781335388483E-6	8.816412195403897E-4	0.0	0.04226627384992637	4.3753906676942415E-6
50-51	8.750781335388483E-6	9.472720795558033E-4	0.0	0.04960599169498346	4.3753906676942415E-6
52-53	8.750781335388483E-6	9.735244235619688E-4	0.0	0.05665255836530504	4.3753906676942415E-6
54-55	8.750781335388483E-6	0.0010172783302389112	0.0	0.06290717932477397	4.3753906676942415E-6
56-57	8.750781335388483E-6	0.0010369675882435352	0.0	0.06810514343799473	4.3753906676942415E-6
58-59	8.750781335388483E-6	0.001050093760246618	0.0	0.07318059661252004	4.3753906676942415E-6
60-61	8.750781335388483E-6	0.0010741584089189364	0.0	0.07851857322710701	4.3753906676942415E-6
62-63	8.750781335388483E-6	0.0010960353622574076	0.0	0.08380404515368166	4.3753906676942415E-6
64-65	1.0938476669235603E-5	0.0012207339962866934	0.0	0.08870010731083153	4.3753906676942415E-6
66-67	1.3126172003082725E-5	0.0012382355589574704	0.0	0.09359616946798138	4.3753906676942415E-6
68-69	1.3126172003082725E-5	0.0012973033329713429	0.0	0.09828221287308189	4.3753906676942415E-6
70-71	1.3126172003082725E-5	0.001321367981643661	0.0	0.10260072346209612	4.3753906676942415E-6
72-73	1.3126172003082725E-5	0.0013279310676452025	0.0	0.10620385767694232	4.3753906676942415E-6
74-75	1.5313867336929844E-5	0.0013716849743221449	0.0	0.10899098153226355	4.3753906676942415E-6
76-77	1.7501562670776966E-5	0.0014591927876760297	0.0	0.11167747140222783	4.3753906676942415E-6
78-79	1.7501562670776966E-5	0.0015051343896868193	0.0	0.11334887063728702	4.3753906676942415E-6
80-81	1.7501562670776966E-5	0.0015051343896868193	0.0	0.1138629790407411	4.3753906676942415E-6
82-83	1.7501562670776966E-5	0.0015160728663560549	0.0	0.11435739818619055	4.3753906676942415E-6
84-85	1.7501562670776966E-5	0.0015248236476914433	0.0	0.11455866615690449	4.3753906676942415E-6
86-87	1.7501562670776966E-5	0.0015663898590345384	0.0	0.11467680170493223	4.3753906676942415E-6
88	1.7501562670776966E-5	0.0016582730630561177	0.0	0.11468774018160147	4.3753906676942415E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	28155	0.0	49.93591	1
GTATCAA	51525	0.0	36.33048	1
TCAACGC	64795	0.0	28.39256	4
ATCAACG	65230	0.0	28.216106	3
CAACGCA	66010	0.0	27.831743	5
TATCAAC	67740	0.0	27.32337	2
AACGCAG	67385	0.0	27.313805	6
ACGCAGA	77350	0.0	23.74605	7
CGCAGAG	79085	0.0	23.256994	8
GCAGAGT	91265	0.0	19.895302	9
GTACATG	64680	0.0	19.83659	1
TACATGG	65350	0.0	19.182362	2
AGAGTAC	83370	0.0	18.814583	10-11
ACATGGG	65475	0.0	18.485657	3
GAGTACT	55320	0.0	18.33808	12-13
CAGAGTA	89660	0.0	17.69395	10-11
CATGGGG	46700	0.0	16.6902	4
AGTACTT	61665	0.0	16.567091	12-13
GTACTTT	63445	0.0	15.980432	14-15
GTGGTAT	14085	0.0	15.301539	1
>>END_MODULE