FastQCFastQC Report
Fri 27 May 2016
SRR2139532_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139532_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22058713
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA273050.050.9321821
GTATCAA506350.036.7808571
TCAACGC624850.029.045944
ATCAACG629550.028.8424343
CAACGCA639600.028.362915
TATCAAC662000.027.7456532
AACGCAG657000.027.6629416
ACGCAGA754200.024.0922187
CGCAGAG766250.023.7188268
GTACATG677300.020.2599331
GCAGAGT887300.020.2227339
TACATGG686250.019.7201182
ACATGGG692600.018.8683323
AGAGTAC817550.018.60426510-11
GAGTACT518050.018.2060612-13
CAGAGTA881100.017.84148610-11
GTGGTAT129450.016.7253071
AGTACTT571850.016.35002712-13
GTACTTT587300.016.06070514-15
CATGGGG458750.016.0099564
Produced by FastQC (version 0.11.5)