FastQCFastQC Report
Fri 27 May 2016
SRR2139531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20047748
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA242050.048.572021
GTATCAA438850.035.6338731
TCAACGC538200.028.3898474
ATCAACG542050.028.149493
CAACGCA546400.027.9329415
AACGCAG562900.027.1963886
TATCAAC570600.026.992992
ACGCAGA645100.023.6462387
CGCAGAG660050.023.1170258
GTACATG565150.019.8794881
GCAGAGT766600.019.6133889
TACATGG579650.018.8157672
AGAGTAC701850.018.40669810-11
ACATGGG580650.018.3491133
GAGTACT452800.018.2606112-13
CAGAGTA754050.017.55904210-11
GTGGTAT120450.016.0757541
AGTACTT502950.016.06779912-13
CATGGGG426050.016.0070784
GTACTTT516800.015.94744614-15
Produced by FastQC (version 0.11.5)