##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139531_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20047748
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.429441651002396	31.0	31.0	34.0	30.0	34.0
2	31.589916533268475	31.0	31.0	34.0	30.0	34.0
3	31.636070046371294	31.0	31.0	34.0	30.0	34.0
4	35.42947571966687	37.0	35.0	37.0	33.0	37.0
5	35.24278587300678	37.0	35.0	37.0	32.0	37.0
6	35.23541671613191	37.0	35.0	37.0	32.0	37.0
7	35.14597140786087	37.0	35.0	37.0	32.0	37.0
8	35.079599464239074	37.0	35.0	37.0	32.0	37.0
9	36.62078568625264	39.0	35.0	39.0	32.0	39.0
10-11	36.553595421291206	39.0	35.0	39.0	32.0	39.0
12-13	36.50306146605594	38.5	35.0	39.0	32.0	39.0
14-15	37.5814605211518	39.0	36.0	41.0	32.0	41.0
16-17	37.548669331837175	39.0	36.0	41.0	32.0	41.0
18-19	37.55932344620453	39.0	36.0	41.0	32.0	41.0
20-21	37.52633807426427	39.0	36.0	41.0	32.0	41.0
22-23	37.44517195302116	39.0	36.0	41.0	32.0	41.0
24-25	37.31763773126293	39.0	36.0	41.0	31.0	41.0
26-27	37.07244046777939	39.0	36.0	40.5	31.0	41.0
28-29	36.90548819720845	39.0	36.0	40.0	30.5	41.0
30-31	36.72713270964857	38.5	35.5	40.0	30.0	41.0
32-33	36.49563901845609	38.0	35.0	40.0	30.0	41.0
34-35	36.33389617475599	38.0	35.0	40.0	30.0	41.0
36-37	36.16807852356222	38.0	35.0	40.0	30.0	41.0
38-39	35.989095233254375	38.0	34.0	40.0	29.5	41.0
40-41	35.816806635529275	38.0	34.0	40.0	29.0	41.0
42-43	35.59465719964294	38.0	34.0	40.0	28.0	41.0
44-45	36.0430602529336	38.0	34.5	40.0	29.5	41.0
46-47	36.29588321990935	38.0	35.0	40.0	30.0	41.0
48-49	36.343256668551724	38.0	35.0	40.0	30.0	41.0
50-51	36.261988074501694	38.0	35.0	40.0	29.0	41.0
52-53	36.12085772238906	38.0	34.0	40.0	29.0	41.0
54-55	35.98222455422213	38.0	34.0	40.0	28.5	41.0
56-57	35.850647739138125	38.0	34.0	40.0	28.0	41.0
58-59	35.66116986432881	38.0	34.0	40.0	28.0	41.0
60-61	35.46806684232378	37.5	33.5	40.0	28.0	41.0
62-63	35.23452908594416	37.0	33.0	40.0	28.0	41.0
64-65	34.96409205333376	36.5	33.0	39.5	27.5	41.0
66-67	34.653976312621765	36.0	32.5	39.0	26.5	41.0
68-69	34.37881868755342	36.0	32.0	39.0	26.0	40.0
70-71	34.086608211512925	35.0	32.0	38.5	26.0	40.0
72-73	33.77520457879508	35.0	31.5	38.0	26.0	40.0
74-75	33.444608325350444	35.0	31.0	37.5	26.0	39.5
76-77	31.76442499923597	33.5	29.5	35.5	25.0	38.5
78-79	32.78677468370326	34.0	31.0	36.0	26.0	39.0
80-81	32.86378589855877	34.0	31.0	36.0	26.0	38.5
82-83	32.64037451053264	34.0	31.0	36.0	26.0	37.5
84-85	32.40450427625176	34.0	31.0	35.0	26.0	37.0
86-87	32.19863158801378	34.0	31.0	35.0	26.0	36.5
88-89	31.991593463503673	34.0	31.0	35.0	25.5	36.0
90-91	31.842339951684785	34.0	31.0	35.0	25.0	36.0
92-93	31.806342590464048	34.0	31.0	35.0	25.0	35.5
94-95	31.803178414309045	34.0	31.0	35.0	25.0	35.0
96-97	31.919634461014667	34.0	31.0	35.0	26.0	35.0
98-99	32.14621436949558	34.0	31.0	35.0	26.5	35.0
100	30.182221229076283	32.0	27.0	34.0	23.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	9.0
19	35.0
20	314.0
21	1977.0
22	9140.0
23	30259.0
24	76446.0
25	153075.0
26	260881.0
27	391104.0
28	533905.0
29	677635.0
30	818281.0
31	968462.0
32	1137853.0
33	1356667.0
34	1650442.0
35	2051927.0
36	2647336.0
37	3298394.0
38	2890793.0
39	1065996.0
40	26817.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.5078689137553	21.87577876577459	15.225485675498316	23.390866644971794
2	14.399740235146721	22.61119809447179	41.02768021835909	21.961381452022408
3	17.373933209249557	28.090664157456903	31.454643329978342	23.080759303315197
4	12.42762520074598	19.695008770072853	39.719376623501	28.157989405680166
5	12.456755741343118	38.76316182745314	36.33727339350036	12.442809037703388
6	28.1596865642964	39.79138205448313	19.482327890394473	12.566603490826003
7	24.955930212211367	33.461464100606214	24.72374453230358	16.85886115487884
8	23.806552565578528	37.386625511529495	21.790732312701895	17.016089610190086
9	25.293504287863154	16.91153041229369	21.910660489148206	35.88430481069494
10-11	23.549300166978867	27.251254897900868	29.764504953212573	19.434939981907693
12-13	24.571397417219902	24.88937093827316	29.758381475297075	20.78085016920986
14-15	21.722112629284023	26.06764700490793	27.302755469494027	24.90748489631402
16-17	21.192414107269407	29.123702167453146	27.906240183523146	21.777643541754298
18-19	21.295335742132988	28.400419309015035	29.5502843225082	20.753960626343776
20-21	22.08615729416962	27.567621914694335	29.37933670791914	20.966884083216904
22-23	21.854991227669498	27.4960503994205	29.224527181127613	21.424431191782386
24-25	21.657124853870478	27.695193375132327	29.196532093988292	21.451149677008903
26-27	21.64006945607309	27.77190165340473	29.101987750349075	21.48604114017311
28-29	21.6175211469318	27.774367818703034	29.149684658130546	21.45842637623462
30-31	21.834699189374547	27.71294732636132	29.007382689120913	21.444970795143213
32-33	21.679083472736615	27.84809666203916	28.962994338612663	21.50982552661156
34-35	21.771629327060666	27.864137582976333	28.987712205647025	21.376520884315976
36-37	21.801758135453476	27.841331077475196	28.872866136428925	21.4840446506424
38-39	21.721407125663735	27.882788504446516	28.89571992996625	21.5000844399235
40-41	21.871805760081955	27.818171511420807	28.76836364778464	21.541659080712595
42-43	21.775367437550752	27.959815798198168	28.5523565327866	21.71246023146448
44-45	21.84141094885554	28.03634397356155	28.3913547667101	21.730890310872812
46-47	21.908136290050393	28.067285643456973	28.157746328181858	21.866831738310776
48-49	21.88530414483319	28.149783398495032	28.098665865651107	21.866246591020673
50-51	21.917749162670404	28.25020907625949	27.947501567306855	21.88454019376325
52-53	22.047146553382987	28.165760517929545	27.854713146766596	21.932379781920876
54-55	22.017867040667873	28.122002326300198	27.92886200373821	21.93126862929372
56-57	21.908232450622158	28.200886135538294	27.96585058569589	21.925030828143658
58-59	21.931981702738167	28.250079776257405	27.947187141280143	21.870751379724286
60-61	22.03060796322096	28.221199064532726	27.924627576850703	21.823565395395615
62-63	21.984865985697127	28.303892410938925	27.901282177200976	21.809959426162973
64-65	21.920898838630333	28.217438557235297	27.908938905806085	21.95272369832829
66-67	21.97509452257879	28.226271588110823	27.843507315101373	21.955126574209014
68-69	21.892240858074928	28.203642041491012	27.911198475261028	21.99291862517304
70-71	21.904759175872744	28.039935499585972	28.004633419888826	22.050671904652457
72-73	21.95663065307363	28.11614987373702	27.879732258085898	22.04748721510346
74-75	21.8366107528489	28.165868556595147	27.958660569242262	22.03886012131369
76-77	21.89500671631457	28.100299590354812	27.98387236063079	22.020821332699832
78-79	21.837286844198893	28.102865320852633	27.96101025744807	22.0988375775004
80-81	21.895845410285897	28.101251927847375	27.992842399251778	22.010060262614957
82-83	21.89741124985515	28.07791761432118	28.074816586327245	21.949854549496425
84-85	21.914040901803606	28.085824740520138	28.03188973047978	21.968244627196476
86-87	21.794611705059253	28.111821349529563	28.13320380768985	21.96036313772133
88-89	21.884437835848146	28.028990383814968	28.09513518923839	21.9914365910985
90-91	21.855595237805485	28.08641612220763	28.141260921971373	21.916727718015515
92-93	21.88496825936643	27.964113599413515	28.22638472924372	21.924533411976334
94-95	21.613368057284454	27.983309202821477	28.38964267960652	22.01368006028755
96-97	21.663733943470774	28.15460421412894	28.478113185299176	21.70354865710111
98-99	22.709467509585277	30.061007629493382	28.82891170301236	18.40061315790898
100	22.385120580866737	30.39892951150735	22.59786007757569	24.618089830050224
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3698.0
1	3462.0
2	5725.0
3	8604.0
4	11097.5
5	17726.0
6	23803.0
7	28434.5
8	31842.5
9	31335.5
10	27746.5
11	23676.0
12	24311.0
13	31608.5
14	49453.0
15	77927.0
16	102748.0
17	116819.0
18	122145.0
19	119355.5
20	113377.0
21	109213.0
22	107946.0
23	115292.0
24	134939.5
25	165259.0
26	202644.5
27	242569.5
28	287671.0
29	338947.5
30	389865.0
31	437346.5
32	487140.5
33	534210.0
34	576158.5
35	615945.0
36	658346.0
37	696362.0
38	725742.0
39	749906.5
40	769262.5
41	783011.5
42	786785.0
43	791714.0
44	797474.0
45	804493.0
46	806459.0
47	799444.5
48	791177.5
49	777353.0
50	759739.5
51	735043.0
52	702359.5
53	664396.5
54	622895.5
55	578480.5
56	531906.5
57	484770.5
58	433956.0
59	376705.5
60	314750.0
61	255764.0
62	204300.5
63	159080.0
64	120298.0
65	90919.5
66	69008.5
67	53293.5
68	41729.5
69	31511.5
70	23199.0
71	17059.5
72	12222.5
73	8666.5
74	6129.5
75	4232.0
76	2919.5
77	2004.0
78	1384.0
79	985.5
80	708.5
81	504.5
82	379.0
83	283.0
84	212.5
85	154.0
86	112.0
87	85.0
88	50.5
89	26.0
90	19.0
91	16.5
92	15.5
93	10.0
94	3.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.11714033915430301
3	9.976182861037558E-6
4	1.1971419433245071E-4
5	0.0
6	0.0
7	0.0
8	1.3966656005452583E-4
9	0.0
10-11	7.4821371457781695E-6
12-13	4.7386868589928406E-5
14-15	3.7660090300416785E-4
16-17	7.008268459878886E-4
18-19	1.745832000681573E-5
20-21	7.482667523082906E-5
22-23	2.4946552012313616E-6
24-25	0.00648840100993459
26-27	1.9976432803400188E-5
28-29	0.0023865395371632687
30-31	0.0022194918274532523
32-33	0.023637348605678545
34-35	0.015919907100766396
36-37	0.00848651681292067
38-39	0.0043087246370464094
40-41	9.217966373114016E-4
42-43	3.1108992385907E-4
44-45	0.0010838798261467132
46-47	0.0018450875486190385
48-49	0.0012168179229414286
50-51	5.3223737850950005E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	4.1252895540738006E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	8.381617955737832E-5
70-71	4.8535007350568386E-4
72-73	1.183567397594494E-5
74-75	4.491500598672115E-5
76-77	2.9392312128658817E-4
78-79	2.053778276120435E-4
80-81	2.1742780791085608E-5
82-83	3.1514511140285147E-6
84-85	3.1989921895006195E-6
86-87	9.426736121991587E-5
88-89	5.262694512830052E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	863.0
20-21	2889.0
22-23	5537.0
24-25	11460.0
26-27	14249.0
28-29	21313.0
30-31	40861.0
32-33	50927.0
34-35	70115.0
36-37	95651.0
38-39	121253.0
40-41	129232.0
42-43	153348.0
44-45	183119.0
46-47	164678.0
48-49	156196.0
50-51	147568.0
52-53	141347.0
54-55	152869.0
56-57	162041.0
58-59	168923.0
60-61	173493.0
62-63	168361.0
64-65	174527.0
66-67	188551.0
68-69	196533.0
70-71	203473.0
72-73	200339.0
74-75	203034.0
76-77	182775.0
78-79	208476.0
80-81	229124.0
82-83	235076.0
84-85	242947.0
86-87	266605.0
88-89	303823.0
90-91	346542.0
92-93	404488.0
94-95	506353.0
96-97	982191.0
98-99	1636760.0
100-101	1.0999838E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.0159248989752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.26515779338419	72.39148552778394
2	7.691792120545521	12.616988897943815
3	2.114780095322994	5.2033693642757495
4	0.8415372647375876	2.7607782841762805
5	0.40552325853362203	1.6629682558340624
6	0.22611765351524074	1.112714909342309
7	0.13644075660826047	0.7833220392999711
8	0.08327207451821017	0.5463708967893913
9	0.05532992060037019	0.40841411513636006
>10	0.17557185617384083	2.1877718522705663
>50	0.0036119891255115186	0.1955725039134054
>100	8.533246289546245E-4	0.12036297202113425
>500	0.0	0.0
>1k	1.1892305823699898E-5	0.00988038121309275
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.988091430518779E-6
2	0.0	0.0	0.0	0.0	4.988091430518779E-6
3	0.0	0.0	0.0	4.988091430518779E-6	4.988091430518779E-6
4	0.0	1.4964274291556339E-5	0.0	9.976182861037558E-6	1.4964274291556339E-5
5	4.988091430518779E-6	1.4964274291556339E-5	0.0	2.9928548583112678E-5	1.4964274291556339E-5
6	2.49404571525939E-5	1.4964274291556339E-5	0.0	3.491664001363146E-5	1.4964274291556339E-5
7	2.49404571525939E-5	1.4964274291556339E-5	0.0	4.98809143051878E-5	1.4964274291556339E-5
8	2.49404571525939E-5	2.49404571525939E-5	0.0	4.98809143051878E-5	1.9952365722075116E-5
9	2.49404571525939E-5	1.0474992004089436E-4	0.0	4.98809143051878E-5	2.9928548583112678E-5
10-11	3.2422594298372066E-5	1.0474992004089436E-4	0.0	4.98809143051878E-5	2.9928548583112678E-5
12-13	3.491664001363146E-5	1.0973801147141315E-4	0.0	5.7363051450965964E-5	2.9928548583112678E-5
14-15	3.990473144415023E-5	1.6710106292237912E-4	0.0	5.9857097166225356E-5	4.4892822874669015E-5
16-17	6.484518859674413E-5	1.8455938292919483E-4	0.0	5.9857097166225356E-5	6.983328002726291E-5
18-19	6.484518859674413E-5	1.895474743597136E-4	0.0	5.9857097166225356E-5	7.23273257425223E-5
20-21	6.484518859674413E-5	1.945355657902324E-4	0.0	6.235114288148475E-5	7.48213714577817E-5
22-23	6.733923431200353E-5	2.394283886649014E-4	0.0	7.23273257425223E-5	8.729160003407865E-5
24-25	6.983328002726291E-5	2.668628915327547E-4	0.0	8.729160003407863E-5	1.0724396575615376E-4
26-27	6.983328002726291E-5	2.7434502867853286E-4	0.0	1.3717251433926643E-4	1.0973801147141315E-4
28-29	6.983328002726291E-5	2.7434502867853286E-4	0.0	3.39190217275277E-4	1.0973801147141315E-4
30-31	7.980946288830047E-5	3.1923785155320186E-4	0.0	0.0011248146175819847	1.1223205718667253E-4
32-33	8.729160003407863E-5	3.2422594298372066E-4	0.0	0.002920527532568745	1.1971419433245071E-4
34-35	9.97618286103756E-5	3.541544915668333E-4	0.0	0.0051427222648648616	1.1971419433245071E-4
36-37	1.0973801147141315E-4	3.84083040149946E-4	0.0	0.008098166437447239	1.247022857629695E-4
38-39	1.0973801147141315E-4	4.065294515872805E-4	0.0	0.01311868046226439	1.2969037719348826E-4
40-41	1.0973801147141315E-4	4.539163201772089E-4	0.0	0.01850831325293993	1.3467846862400706E-4
42-43	1.0973801147141315E-4	4.564103658924683E-4	0.0	0.023725856889262573	1.3717251433926646E-4
44-45	1.197141943324507E-4	4.86338914475581E-4	0.0	0.029716554697315628	1.3966656005452583E-4
46-47	1.3467846862400706E-4	5.137734173434343E-4	0.0	0.0360314784483524	1.47148697200304E-4
48-49	1.3467846862400706E-4	5.162674630586937E-4	0.0	0.04241623547941644	1.5463083434608216E-4
50-51	1.496427429155634E-4	5.611602859333627E-4	0.0	0.04886833174479248	1.5463083434608216E-4
52-53	1.496427429155634E-4	5.661483773638815E-4	0.0	0.055285511370154895	1.7458320006815726E-4
54-55	1.496427429155634E-4	5.761245602249191E-4	0.0	0.06128618536106899	1.970296115054918E-4
56-57	1.496427429155634E-4	5.836066973706972E-4	0.0	0.06676560379749386	2.0700579436652936E-4
58-59	1.496427429155634E-4	5.836066973706972E-4	0.0	0.07236723047396645	2.1448793151230752E-4
60-61	1.6211297149186033E-4	5.960769259469942E-4	0.0	0.07808857134477148	2.1448793151230752E-4
62-63	1.7957129149867606E-4	6.060531088080317E-4	0.0	0.08353307314118275	2.1448793151230752E-4
64-65	1.7957129149867606E-4	6.733923431200352E-4	0.0	0.08855109312028464	2.1698197722756692E-4
66-67	1.8954747435971363E-4	6.808744802658134E-4	0.0	0.09340949417360991	2.194760229428263E-4
68-69	1.8954747435971363E-4	6.983328002726291E-4	0.0	0.09830031782123358	2.194760229428263E-4
70-71	2.0201770293601056E-4	7.132970745641854E-4	0.0	0.1025676300400424	2.2945220580386386E-4
72-73	2.444164800954202E-4	7.23273257425223E-4	0.0	0.10626131174434156	2.5439266295645773E-4
74-75	2.444164800954202E-4	7.457196688625575E-4	0.0	0.10911200599688305	2.5439266295645773E-4
76-77	2.444164800954202E-4	7.781422631609296E-4	0.0	0.11162849812357976	2.5688670867171713E-4
78-79	2.444164800954202E-4	7.93106537452486E-4	0.0	0.11339428248998341	2.593807543869765E-4
80-81	2.444164800954202E-4	7.93106537452486E-4	0.0	0.11398287727878463	2.618748001022359E-4
82-83	2.444164800954202E-4	7.93106537452486E-4	0.0	0.11447420428469073	2.693569372480141E-4
84-85	2.469105258106796E-4	7.956005831677454E-4	0.0	0.11472859694764719	2.693569372480141E-4
86-87	2.49404571525939E-4	8.180469946050798E-4	0.0	0.11484332305054912	2.693569372480141E-4
88	2.5439266295645773E-4	8.579517260492301E-4	0.0	0.1148508051876949	2.693569372480141E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	24205	0.0	48.57202	1
GTATCAA	43885	0.0	35.633873	1
TCAACGC	53820	0.0	28.389847	4
ATCAACG	54205	0.0	28.14949	3
CAACGCA	54640	0.0	27.932941	5
AACGCAG	56290	0.0	27.196388	6
TATCAAC	57060	0.0	26.99299	2
ACGCAGA	64510	0.0	23.646238	7
CGCAGAG	66005	0.0	23.117025	8
GTACATG	56515	0.0	19.879488	1
GCAGAGT	76660	0.0	19.613388	9
TACATGG	57965	0.0	18.815767	2
AGAGTAC	70185	0.0	18.406698	10-11
ACATGGG	58065	0.0	18.349113	3
GAGTACT	45280	0.0	18.26061	12-13
CAGAGTA	75405	0.0	17.559042	10-11
GTGGTAT	12045	0.0	16.075754	1
AGTACTT	50295	0.0	16.067799	12-13
CATGGGG	42605	0.0	16.007078	4
GTACTTT	51680	0.0	15.947446	14-15
>>END_MODULE